MD07G1050100.v1.1

hydroxyproline O-arabinosyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002578
Physical Location & Seq
Forward (+)
4282897 .. 4287341
4445 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1050100.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGATTGAGAGGAAGAAGATGGGACGAGCTTCACCGTTAGTTCTGGTCCTTTTGGCTCTTGGGTTTTTCTTTGCTACTTATAATTTGTCAACTATGGTAATTCATTATAGATTGGTTGGGAAATGGGTAGGTGACAACACGAATAGTGGATTACTGTCTGACCCGATAATTGAGATGCCGGAGAATGCGAAGAAGTTTAAGAATACCAAGGCACCATTCCATGTTGCCTTAACAGCCACTGATACTCCTTACAGCAAATGGCAGTGTCGCATTATGTACTATTGGTATAAGAAGAAGAAGGACTTGCCTGCATCAGAGATGGGAGGATTTACTCGAATTTTGCATTCAGGAAAACCTGACAACTTGATGGATGAGATTCCAACTACTGTGGTTGATCCTCTTCCTGGAGGACTTGATCGGGGATACATTGTCTTAAACAGACCATGGGCTTTTGTGCAATGGCTGGAAAAGGCTACTATTGAGGAAGAATATATTTTGATGGCGGAGCCTGATCACATTTTTATAAATCCTCTTCCTAATTTGGCACTTGGAGGATATCCAGCAGCCTTCCCATTTTTCTACATTAAACCTTCTCAGCATGAGAAGATCATAAGAAAGTTTTATCCTGAGGGGAACGGTCCGGTCACAAAGATTGGTCCAATTGGAAATTCTCCTGTAATTATCAAGAAGGATTTGCTGGAAAAGATTGCTCCTACATGGATGAATGTTTCTTTGAGGATGAAAGAAGACACGGAGACGGATAAAGCTTTCGGATGGGTGCTAGAAATGTATGCTTATGCTGTGGCATCTGCTTTGCATGGTGTGCCGCATATTCTTCGGAAGGAGTTCATGGTGCAGCCGCCATGGGATACGGAACTTCGTAAAAAGTTTATCATTCATTATACTTACGGATGTGACTACAACTTAAAGGGTGTGCTAACATATGGAAAAATTGGGGAATGGAGATTTGACAAGAGAGCATATCTACGTGGACCTCCACCAAGAAACCTCCCCTTACCTCCTCCTGGGGTTCCTGAGAGTGTGGTCACCCTTGTAAAGATGGTGAATGAAGCTACTGCTAACATTCCAAATTGGGACACACAATAG

Protein Analysis

369

Amino Acids

41.99

Weight (kDa)

9.14

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HPAT PF23452 73 - 366 9.4e-129 Hydroxyproline O-arabinosyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 81, 524
AccB1I GGYRCC 1 cut(s) 211
AciI CCGC 3 cut(s) 503, 827, 860
AclWI GGATC 1 cut(s) 389
AcsI RAATTY 2 cut(s) 336, 667
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 2 cut(s) 404, 1025
AjnI CCWGG 2 cut(s) 403, 1024
AjuI GAANNNNNNNTTGG 4 cut(s) 200, 232, 652, 684
AluBI AGCT 3 cut(s) 29, 767, 1073
AluI AGCT 3 cut(s) 29, 767, 1073
Alw26I GTCTC 1 cut(s) 749
AlwI GGATC 1 cut(s) 389
AlwNI CAGNNNCTG 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 563, 856
ApoI RAATTY 2 cut(s) 336, 667
Asp700I GAANNNNTTC 1 cut(s) 845
AspS9I GGNCC 4 cut(s) 46, 638, 656, 992
AsuHPI GGTGA 4 cut(s) 24, 143, 1039, 1075
AvaII GGWCC 4 cut(s) 46, 638, 656, 992
AxyI CCTNAGG 1 cut(s) 627
BanI GGYRCC 1 cut(s) 211
BbsI GAAGAC 1 cut(s) 753
BbvI GCAGC 2 cut(s) 575, 868
BccI CCATC 6 cut(s) 13, 313, 361, 493, 768, 1054
BcgI CGANNNNNNTGC 2 cut(s) 818, 852
BciT130I CCWGG 2 cut(s) 405, 1026
BciVI GTATCC 2 cut(s) 416, 862
BclI TGATCA 1 cut(s) 511
BcoDI GTCTC 1 cut(s) 749
BfaI CTAG 1 cut(s) 782
BfuI GTATCC 2 cut(s) 416, 862
BisI GCNGC 4 cut(s) 564, 827, 857, 860
BlsI GCNGC 4 cut(s) 565, 828, 858, 861
Bme1390I CCNGG 2 cut(s) 405, 1026
Bme18I GGWCC 4 cut(s) 46, 638, 656, 992
BmgT120I GGNCC 4 cut(s) 46, 638, 656, 992
BmiI GGNNCC 3 cut(s) 213, 507, 1032
BmrFI CCNGG 2 cut(s) 405, 1026
BmsI GCATC 3 cut(s) 165, 320, 815
BpiI GAAGAC 1 cut(s) 753
BpmI CTGGAG 1 cut(s) 426
BsaAI YACGTR 1 cut(s) 989
BsaJI CCNNGG 4 cut(s) 207, 443, 863, 1025
BsaWI WCCGGW 1 cut(s) 640
BsaXI ACNNNNNCTCC 2 cut(s) 229, 259
Bsc4I CCNNNNNNNGG 2 cut(s) 404, 1025
Bse21I CCTNAGG 1 cut(s) 627
Bse3DI GCAATG 1 cut(s) 464
BseBI CCWGG 2 cut(s) 405, 1026
BseDI CCNNGG 4 cut(s) 207, 443, 863, 1025
BseGI GGATG 5 cut(s) 376, 726, 744, 779, 917
BseLI CCNNNNNNNGG 2 cut(s) 404, 1025
BseMI GCAATG 1 cut(s) 464
BseMII CTCAG 3 cut(s) 608, 618, 1026
BseRI GAGGAG 1 cut(s) 1011
BseXI GCAGC 2 cut(s) 575, 868
BsgI GTGCAG 1 cut(s) 875
BshNI GGYRCC 1 cut(s) 211
BsiSI CCGG 2 cut(s) 179, 641
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 404, 1025
BsmAI GTCTC 1 cut(s) 749
BsmBI CGTCTC 1 cut(s) 749
BsmFI GGGAC 1 cut(s) 36
BsmI GAATGC 2 cut(s) 190, 343
Bsp143I GATC 4 cut(s) 394, 415, 511, 606
Bsp19I CCATGG 2 cut(s) 443, 863
BspACI CCGC 3 cut(s) 503, 827, 860
BspCNI CTCAG 3 cut(s) 607, 619, 1027
BspLI GGNNCC 3 cut(s) 213, 507, 1032
BspPI GGATC 1 cut(s) 389
BspT107I GGYRCC 1 cut(s) 211
BsrDI GCAATG 1 cut(s) 464
BssECI CCNNGG 4 cut(s) 207, 443, 863, 1025
BssMI GATC 4 cut(s) 394, 415, 511, 606
BssT1I CCWWGG 3 cut(s) 207, 443, 863
Bst2UI CCWGG 2 cut(s) 405, 1026
Bst4CI ACNGT 4 cut(s) 36, 156, 388, 638
Bst6I CTCTTC 2 cut(s) 405, 537
BstAPI GCANNNNNTGC 1 cut(s) 823
BstBAI YACGTR 1 cut(s) 989
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 3 cut(s) 594, 627, 1035
BstDSI CCRYGG 2 cut(s) 443, 863
BstEII GGTNACC 1 cut(s) 1045
BstF5I GGATG 5 cut(s) 376, 726, 744, 779, 917
BstKTI GATC 4 cut(s) 397, 418, 514, 609
BstMAI GTCTC 1 cut(s) 749
BstMBI GATC 4 cut(s) 394, 415, 511, 606
BstMWI GCNNNNNNNGC 2 cut(s) 233, 823
BstNI CCWGG 2 cut(s) 405, 1026
BstPI GGTNACC 1 cut(s) 1045
BstSCI CCNGG 2 cut(s) 403, 1024
BstV1I GCAGC 2 cut(s) 575, 868
BstV2I GAAGAC 1 cut(s) 753
Bsu36I CCTNAGG 1 cut(s) 627
BsuI GTATCC 2 cut(s) 416, 862
BtgI CCRYGG 2 cut(s) 443, 863
BtsCI GGATG 5 cut(s) 376, 726, 744, 779, 917
BtsI GCAGTG 1 cut(s) 269
BtsIMutI CAGTG 2 cut(s) 237, 269
Cac8I GCNNGC 1 cut(s) 309
CaiI CAGNNNCTG 1 cut(s) 239
Cfr13I GGNCC 4 cut(s) 46, 638, 656, 992
CpoI CGGWCCG 1 cut(s) 638
Csp6I GTAC 1 cut(s) 277
CspCI CAANNNNNGTGG 2 cut(s) 369, 404
CspI CGGWCCG 1 cut(s) 638
CviAII CATG 7 cut(s) 221, 444, 599, 717, 818, 850, 864
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 3 cut(s) 594, 627, 1035
DpnI GATC 4 cut(s) 396, 417, 513, 608
DpnII GATC 4 cut(s) 394, 415, 511, 606
Eam1104I CTCTTC 2 cut(s) 405, 537
EarI CTCTTC 2 cut(s) 405, 537
EciI GGCGGA 1 cut(s) 518
Eco130I CCWWGG 3 cut(s) 207, 443, 863
Eco32I GATATC 1 cut(s) 557
Eco47I GGWCC 4 cut(s) 46, 638, 656, 992
Eco81I CCTNAGG 1 cut(s) 627
Eco91I GGTNACC 1 cut(s) 1045
EcoO65I GGTNACC 1 cut(s) 1045
EcoRII CCWGG 2 cut(s) 403, 1024
EcoRV GATATC 1 cut(s) 557
EcoT14I CCWWGG 3 cut(s) 207, 443, 863
ErhI CCWWGG 3 cut(s) 207, 443, 863
Esp3I CGTCTC 1 cut(s) 749
FaeI CATG 7 cut(s) 224, 447, 602, 720, 821, 853, 867
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 1 cut(s) 36
FatI CATG 7 cut(s) 220, 443, 598, 716, 817, 849, 863
FauNDI CATATG 1 cut(s) 943
FbaI TGATCA 1 cut(s) 511
Fnu4HI GCNGC 4 cut(s) 564, 827, 857, 860
FokI GGATG 5 cut(s) 383, 733, 751, 786, 924
Fsp4HI GCNGC 4 cut(s) 564, 827, 857, 860
FspBI CTAG 1 cut(s) 782
GluI GCNGC 4 cut(s) 564, 827, 857, 860
GsuI CTGGAG 1 cut(s) 426
HapII CCGG 2 cut(s) 179, 641
Hin1II CATG 7 cut(s) 224, 447, 602, 720, 821, 853, 867
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HindIII AAGCTT 1 cut(s) 765
HinfI GANTC 1 cut(s) 376
HpaII CCGG 2 cut(s) 179, 641
HphI GGTGA 4 cut(s) 24, 143, 1039, 1075
Hpy166II GTNNAC 2 cut(s) 90, 992
Hpy188I TCNGA 4 cut(s) 160, 316, 773, 840
Hpy188III TCNNGA 4 cut(s) 348, 626, 685, 1034
Hpy8I GTNNAC 2 cut(s) 90, 992
HpyAV CCTTC 5 cut(s) 292, 577, 600, 682, 835
HpyCH4III ACNGT 4 cut(s) 36, 156, 388, 638
HpyCH4IV ACGT 1 cut(s) 988
HpyCH4V TGCA 5 cut(s) 311, 343, 457, 817, 856
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 823
HpyF3I CTNAG 3 cut(s) 594, 627, 1035
HpySE526I ACGT 1 cut(s) 988
Hsp92II CATG 7 cut(s) 224, 447, 602, 720, 821, 853, 867
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 4 cut(s) 394, 415, 511, 606
LmnI GCTCC 2 cut(s) 505, 715
Lsp1109I GCAGC 2 cut(s) 575, 868
LweI GCATC 3 cut(s) 165, 320, 815
MaeI CTAG 1 cut(s) 782
MaeII ACGT 1 cut(s) 988
MaeIII GTNAC 4 cut(s) 131, 643, 914, 1045
MalI GATC 4 cut(s) 396, 417, 513, 608
MboI GATC 4 cut(s) 394, 415, 511, 606
MfeI CAATTG 1 cut(s) 660
MmeI TCCRAC 1 cut(s) 404
MroXI GAANNNNTTC 1 cut(s) 845
MseI TTAA 5 cut(s) 198, 230, 434, 585, 926
MspI CCGG 2 cut(s) 179, 641
MspR9I CCNGG 2 cut(s) 405, 1026
MunI CAATTG 1 cut(s) 660
Mva1269I GAATGC 2 cut(s) 190, 343
MvaI CCWGG 2 cut(s) 405, 1026
MwoI GCNNNNNNNGC 2 cut(s) 233, 823
NcoI CCATGG 2 cut(s) 443, 863
NdeI CATATG 1 cut(s) 943
NdeII GATC 4 cut(s) 394, 415, 511, 606
NlaIII CATG 7 cut(s) 224, 447, 602, 720, 821, 853, 867
NlaIV GGNNCC 3 cut(s) 213, 507, 1032
NmuCI GTSAC 4 cut(s) 131, 643, 914, 1045
PctI GAATGC 2 cut(s) 190, 343
PdmI GAANNNNTTC 1 cut(s) 845
PfeI GAWTC 1 cut(s) 376
PfoI TCCNGGA 1 cut(s) 403
PkrI GCNGC 4 cut(s) 565, 828, 858, 861
Ppu21I YACGTR 1 cut(s) 989
PsiI TTATAA 2 cut(s) 81, 524
Psp6I CCWGG 2 cut(s) 403, 1024
PspEI GGTNACC 1 cut(s) 1045
PspGI CCWGG 2 cut(s) 403, 1024
PspN4I GGNNCC 3 cut(s) 213, 507, 1032
PspPI GGNCC 4 cut(s) 46, 638, 656, 992
PstNI CAGNNNCTG 1 cut(s) 239
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
Rsr2I CGGWCCG 1 cut(s) 638
RsrII CGGWCCG 1 cut(s) 638
SaqAI TTAA 5 cut(s) 198, 230, 434, 585, 926
SatI GCNGC 4 cut(s) 564, 827, 857, 860
Sau3AI GATC 4 cut(s) 394, 415, 511, 606
Sau96I GGNCC 4 cut(s) 46, 638, 656, 992
ScrFI CCNGG 2 cut(s) 405, 1026
SfaNI GCATC 3 cut(s) 165, 320, 815
SinI GGWCC 4 cut(s) 46, 638, 656, 992
SsiI CCGC 3 cut(s) 503, 827, 860
SspMI CTAG 1 cut(s) 782
StyD4I CCNGG 2 cut(s) 403, 1024
StyI CCWWGG 3 cut(s) 207, 443, 863
TaaI ACNGT 4 cut(s) 36, 156, 388, 638
TaiI ACGT 1 cut(s) 991
TaqI TCGA 1 cut(s) 334
TatI WGTACW 1 cut(s) 276
TauI GCSGC 2 cut(s) 829, 862
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 5 cut(s) 198, 230, 434, 585, 926
Tru9I TTAA 5 cut(s) 198, 230, 434, 585, 926
TscAI CASTG 2 cut(s) 244, 269
TseFI GTSAC 4 cut(s) 131, 643, 914, 1045
TseI GCWGC 2 cut(s) 563, 856
Tsp45I GTSAC 4 cut(s) 131, 643, 914, 1045
TspDTI ATGAA 6 cut(s) 92, 737, 755, 838, 887, 1083
TspGWI ACGGA 4 cut(s) 767, 773, 887, 924
TspRI CASTG 2 cut(s) 244, 269
VpaK11BI GGWCC 4 cut(s) 46, 638, 656, 992
XapI RAATTY 2 cut(s) 336, 667
XmnI GAANNNNTTC 1 cut(s) 845
XspI CTAG 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.