AT2G32220

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003801
Physical Location & Seq
Forward (+)
13678910 .. 13679523
614 bp
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UTR
Exon/CDS
Intron
AT2G32220.1

Sequence Viewer

Length: 408 bp
ATGGTGAAATGTATGAAGCCAGGAAAAGCCGTGATCCTTTTACAAGGACGATACACCGGTAAAAAGGCGGTGATCGTGAAATCCTTCGATGACGGTACCGTTGAGAAGAAGTACGGTCACTGTCTCGTCGCCGGATTGAAGAAGTACCCAAGCAAAGTCATTCGCAAGGATTCGGCAAAGAAGACGGCTAAGAAATCGCGCGTGAAATGCTTCTTCAAGGTCATCAATTACCAGCACGTGATGCCTACGCGCTACACTCTTGATTTGGATCTGAAGAACGTTGTCTCCGCCGACGCGATCTCGTCCAAGGATAAGAAAGTGACGGCTCTTAAGGAAGCGAAGGCGAAGTTTGAAGAGAGGTTTAAGACTGGCAAGAACAGATGGTTCTTCACCAAGCTTAGGTTTTGA

Protein Analysis

135

Amino Acids

15.47

Weight (kDa)

10.19

Isoelectric Point (pI)

19.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27e PF01777 52 - 135 3.3e-33 Ribosomal L27e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 95
AccII CGCG 4 cut(s) 199, 201, 250, 296
AciI CCGC 2 cut(s) 68, 288
AclI AACGTT 1 cut(s) 279
AclWI GGATC 2 cut(s) 28, 276
AcuI CTGAAG 1 cut(s) 293
AcvI CACGTG 1 cut(s) 238
AfaI GTAC 3 cut(s) 97, 113, 146
AfiI CCNNNNNNNGG 1 cut(s) 399
AflII CTTAAG 1 cut(s) 329
AgeI ACCGGT 1 cut(s) 56
AgsI TTSAA 3 cut(s) 139, 217, 353
AjnI CCWGG 1 cut(s) 19
AloI GAACNNNNNNTCC 2 cut(s) 269, 301
AluBI AGCT 1 cut(s) 397
AluI AGCT 1 cut(s) 397
Alw26I GTCTC 2 cut(s) 128, 289
AlwI GGATC 2 cut(s) 28, 276
AsiGI ACCGGT 1 cut(s) 56
Asp700I GAANNNNTTC 2 cut(s) 83, 209
Asp718I GGTACC 1 cut(s) 95
AspLEI GCGC 2 cut(s) 201, 252
AsuHPI GGTGA 3 cut(s) 16, 82, 382
BaeI ACNNNNGTAYC 2 cut(s) 43, 76
BanI GGYRCC 1 cut(s) 95
BbrPI CACGTG 1 cut(s) 238
BbsI GAAGAC 1 cut(s) 188
BccI CCATC 1 cut(s) 375
BceAI ACGGC 3 cut(s) 14, 201, 339
BciT130I CCWGG 1 cut(s) 21
BcoDI GTCTC 2 cut(s) 128, 289
BfrI CTTAAG 1 cut(s) 329
Bme1390I CCNGG 1 cut(s) 21
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 1 cut(s) 21
BmsI GCATC 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 398
BsaAI YACGTR 1 cut(s) 238
BsaBI GATNNNNATC 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 306
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 373
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 1 cut(s) 21
BseDI CCNNGG 1 cut(s) 306
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 373
Bsh1236I CGCG 4 cut(s) 199, 201, 250, 296
BshNI GGYRCC 1 cut(s) 95
BshTI ACCGGT 1 cut(s) 56
BsiSI CCGG 2 cut(s) 57, 132
BslI CCNNNNNNNGG 1 cut(s) 399
BsmAI GTCTC 2 cut(s) 128, 289
Bsp143I GATC 4 cut(s) 33, 72, 268, 297
BspACI CCGC 2 cut(s) 68, 288
BspFNI CGCG 4 cut(s) 199, 201, 250, 296
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 2 cut(s) 28, 276
BspT107I GGYRCC 1 cut(s) 95
BspTI CTTAAG 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 373
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 4 cut(s) 33, 72, 268, 297
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 4 cut(s) 95, 100, 116, 122
Bst6I CTCTTC 1 cut(s) 348
BstAFI CTTAAG 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 241
BstBAI YACGTR 1 cut(s) 238
BstDEI CTNAG 2 cut(s) 189, 398
BstFNI CGCG 4 cut(s) 199, 201, 250, 296
BstHHI GCGC 2 cut(s) 201, 252
BstKTI GATC 4 cut(s) 36, 75, 271, 300
BstMAI GTCTC 2 cut(s) 128, 289
BstMBI GATC 4 cut(s) 33, 72, 268, 297
BstMWI GCNNNNNNNGC 2 cut(s) 207, 241
BstNI CCWGG 1 cut(s) 21
BstSCI CCNGG 1 cut(s) 19
BstUI CGCG 4 cut(s) 199, 201, 250, 296
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 118
CfoI GCGC 2 cut(s) 201, 252
Cfr10I RCCGGY 1 cut(s) 56
CseI GACGC 1 cut(s) 302
Csp6I GTAC 3 cut(s) 96, 112, 145
CspAI ACCGGT 1 cut(s) 56
CviJI RGCY 5 cut(s) 19, 29, 188, 326, 397
CviKI_1 RGCY 5 cut(s) 19, 29, 188, 326, 397
CviQI GTAC 3 cut(s) 96, 112, 145
DdeI CTNAG 2 cut(s) 189, 398
DpnI GATC 4 cut(s) 35, 74, 270, 299
DpnII GATC 4 cut(s) 33, 72, 268, 297
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
EciI GGCGGA 1 cut(s) 277
Eco130I CCWWGG 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 293
Eco72I CACGTG 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 19
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaiI YATR 1 cut(s) 14
GlaI GCGC 2 cut(s) 200, 251
HapII CCGG 2 cut(s) 57, 132
HgaI GACGC 1 cut(s) 302
HhaI GCGC 2 cut(s) 201, 252
Hin6I GCGC 2 cut(s) 199, 250
HinP1I GCGC 2 cut(s) 199, 250
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 1 cut(s) 170
HpaII CCGG 2 cut(s) 57, 132
HphI GGTGA 3 cut(s) 16, 82, 382
Hpy188I TCNGA 1 cut(s) 273
Hpy188III TCNNGA 2 cut(s) 76, 260
Hpy99I CGWCG 2 cut(s) 131, 296
HpyAV CCTTC 2 cut(s) 94, 334
HpyCH4III ACNGT 4 cut(s) 95, 100, 116, 122
HpyCH4IV ACGT 2 cut(s) 237, 279
HpyF10VI GCNNNNNNNGC 2 cut(s) 207, 241
HpyF3I CTNAG 2 cut(s) 189, 398
HpySE526I ACGT 2 cut(s) 237, 279
HspAI GCGC 2 cut(s) 199, 250
KpnI GGTACC 1 cut(s) 99
Kzo9I GATC 4 cut(s) 33, 72, 268, 297
LpnPI CCDG 6 cut(s) 6, 33, 70, 145, 245, 354
LweI GCATC 1 cut(s) 231
MaeII ACGT 2 cut(s) 237, 279
MaeIII GTNAC 2 cut(s) 116, 319
MalI GATC 4 cut(s) 35, 74, 270, 299
MboI GATC 4 cut(s) 33, 72, 268, 297
MboII GAAGA 7 cut(s) 118, 151, 193, 205, 286, 365, 379
MflI RGATCY 1 cut(s) 268
MluCI AATT 1 cut(s) 226
MnlI CCTC 1 cut(s) 351
MroXI GAANNNNTTC 2 cut(s) 83, 209
MseI TTAA 2 cut(s) 330, 363
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 2 cut(s) 57, 132
MspR9I CCNGG 1 cut(s) 21
MvaI CCWGG 1 cut(s) 21
MvnI CGCG 4 cut(s) 199, 201, 250, 296
MwoI GCNNNNNNNGC 2 cut(s) 207, 241
NdeII GATC 4 cut(s) 33, 72, 268, 297
NlaIV GGNNCC 1 cut(s) 97
NmuCI GTSAC 2 cut(s) 116, 319
PdmI GAANNNNTTC 2 cut(s) 83, 209
PfeI GAWTC 1 cut(s) 170
PinAI ACCGGT 1 cut(s) 56
PmaCI CACGTG 1 cut(s) 238
PmlI CACGTG 1 cut(s) 238
Ppu21I YACGTR 1 cut(s) 238
Psp1406I AACGTT 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 19
PspCI CACGTG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 19
PspN4I GGNNCC 1 cut(s) 97
PsuI RGATCY 1 cut(s) 268
RsaI GTAC 3 cut(s) 97, 113, 146
RsaNI GTAC 3 cut(s) 96, 112, 145
SaqAI TTAA 2 cut(s) 330, 363
Sau3AI GATC 4 cut(s) 33, 72, 268, 297
ScrFI CCNGG 1 cut(s) 21
SetI ASST 6 cut(s) 222, 240, 282, 362, 399, 404
SfaNI GCATC 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 1 cut(s) 226
SsiI CCGC 2 cut(s) 68, 288
StyD4I CCNGG 1 cut(s) 19
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 4 cut(s) 95, 100, 116, 122
TaiI ACGT 2 cut(s) 240, 282
TaqI TCGA 1 cut(s) 87
TasI AATT 1 cut(s) 226
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 2 cut(s) 330, 363
Tru9I TTAA 2 cut(s) 330, 363
TscAI CASTG 1 cut(s) 125
TseFI GTSAC 2 cut(s) 116, 319
Tsp45I GTSAC 2 cut(s) 116, 319
TspDTI ATGAA 1 cut(s) 29
TspRI CASTG 1 cut(s) 125
Vha464I CTTAAG 1 cut(s) 329
XmnI GAANNNNTTC 2 cut(s) 83, 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.