MD07G1202500.v1.1

Belongs to the eukaryotic ribosomal protein eL27 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003801
Physical Location & Seq
Reverse (-)
28038488 .. 28038655
168 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1202500.v1.1.491

Sequence Viewer

Length: 168 bp
ATGCCCACCCGGTACACCCTGGATGTGGATCTCAAGGACGTCGTCAACGTCGAGTCTTTGCAGACCAAGGACAAGAAGGTCACTGCATTGAAGGAGGTGAAAAAGCGGTTCGAGGAGCGGTTCAAGACCGGAAAAAACAGGTGGTTCTTCACCAAGCTCAAGTTCTGA

Protein Analysis

56

Amino Acids

6.68

Weight (kDa)

9.99

Isoelectric Point (pI)

26.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27e PF01777 1 - 55 8.4e-20 Ribosomal L27e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AatII GACGTC 1 cut(s) 42
AccBSI CCGCTC 1 cut(s) 118
AciI CCGC 2 cut(s) 106, 118
AclWI GGATC 1 cut(s) 36
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 1 cut(s) 25
AgsI TTSAA 2 cut(s) 91, 124
AjnI CCWGG 1 cut(s) 18
AjuI GAANNNNNNNTTGG 1 cut(s) 146
AluBI AGCT 1 cut(s) 157
AluI AGCT 1 cut(s) 157
AlwI GGATC 1 cut(s) 36
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 2 cut(s) 109, 142
BciT130I CCWGG 1 cut(s) 20
BcnI CCSGG 1 cut(s) 10
Bme1390I CCNGG 2 cut(s) 10, 20
BmrFI CCNGG 2 cut(s) 10, 20
BpuEI CTTGAG 2 cut(s) 17, 143
BpuMI CCSGG 1 cut(s) 10
BsaBI GATNNNNATC 1 cut(s) 27
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 2 cut(s) 18, 66
BsaWI WCCGGW 1 cut(s) 128
Bsc4I CCNNNNNNNGG 1 cut(s) 25
Bse8I GATNNNNATC 1 cut(s) 27
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 2 cut(s) 18, 66
BseGI GGATG 1 cut(s) 28
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 1 cut(s) 25
BseRI GAGGAG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 10, 129
BslI CCNNNNNNNGG 1 cut(s) 25
Bsp143I GATC 1 cut(s) 28
BspACI CCGC 2 cut(s) 106, 118
BspPI GGATC 1 cut(s) 36
BsrBI CCGCTC 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 18, 66
BssMI GATC 1 cut(s) 28
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 20
BstACI GRCGYC 1 cut(s) 39
BstF5I GGATG 1 cut(s) 28
BstKTI GATC 1 cut(s) 31
BstMBI GATC 1 cut(s) 28
BstNI CCWGG 1 cut(s) 20
BstSCI CCNGG 2 cut(s) 8, 18
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
BtsCI GGATG 1 cut(s) 28
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 81
Csp6I GTAC 1 cut(s) 13
CviJI RGCY 1 cut(s) 157
CviKI_1 RGCY 1 cut(s) 157
CviQI GTAC 1 cut(s) 13
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
FokI GGATG 1 cut(s) 35
HapII CCGG 2 cut(s) 10, 129
Hin1I GRCGYC 1 cut(s) 39
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 1 cut(s) 53
HpaII CCGG 2 cut(s) 10, 129
HphI GGTGA 2 cut(s) 109, 142
Hpy166II GTNNAC 2 cut(s) 15, 46
Hpy188I TCNGA 1 cut(s) 167
Hpy188III TCNNGA 1 cut(s) 124
Hpy8I GTNNAC 2 cut(s) 15, 46
Hpy99I CGWCG 2 cut(s) 44, 53
HpyAV CCTTC 2 cut(s) 70, 85
HpyCH4IV ACGT 2 cut(s) 39, 48
HpyCH4V TGCA 2 cut(s) 61, 86
HpySE526I ACGT 2 cut(s) 39, 48
Hsp92I GRCGYC 1 cut(s) 39
Kzo9I GATC 1 cut(s) 28
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 5 cut(s) 5, 23, 32, 124, 142
MaeII ACGT 2 cut(s) 39, 48
MaeIII GTNAC 1 cut(s) 79
MalI GATC 1 cut(s) 30
MbiI CCGCTC 1 cut(s) 118
MboI GATC 1 cut(s) 28
MboII GAAGA 1 cut(s) 139
MflI RGATCY 1 cut(s) 28
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 2 cut(s) 88, 106
MspI CCGG 2 cut(s) 10, 129
MspR9I CCNGG 2 cut(s) 10, 20
MvaI CCWGG 1 cut(s) 20
NciI CCSGG 1 cut(s) 10
NdeII GATC 1 cut(s) 28
NmuCI GTSAC 1 cut(s) 79
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 48
PflFI GACNNNGTC 1 cut(s) 41
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 18
PsuI RGATCY 1 cut(s) 28
PsyI GACNNNGTC 1 cut(s) 41
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
Sau3AI GATC 1 cut(s) 28
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 10, 20
SetI ASST 6 cut(s) 42, 51, 81, 99, 143, 159
SmlI CTYRAG 2 cut(s) 32, 158
SmoI CTYRAG 2 cut(s) 32, 158
SsiI CCGC 2 cut(s) 106, 118
StyD4I CCNGG 2 cut(s) 8, 18
StyI CCWWGG 1 cut(s) 66
TaiI ACGT 2 cut(s) 42, 51
TaqI TCGA 2 cut(s) 51, 111
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 1 cut(s) 79
Tsp45I GTSAC 1 cut(s) 79
TspRI CASTG 1 cut(s) 88
Tth111I GACNNNGTC 1 cut(s) 41
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.