MD09G1137700.v1.1

Belongs to the eukaryotic ribosomal protein eL27 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003801
Physical Location & Seq
Forward (+)
10703573 .. 10703980
408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1137700.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGTGAAGTTTCTGAAGCCAAACAAGGCGGTGATCAACCTCCAGGGCCGCTACGCCGGGCGCAAGGCCGTCATCGTGCGCGCCTTCGACGAGGGCACCCGCGACCGTCCCTACGGGCACTGTTTGGTTGCGGGGATTAAGAAGTACCCAGCCAAGGTCATCCGCAAGGACTCCGCCAAGAAGACGGCCAAGAAATCTAGAGTGAAGGCCTTCATCAAGCTCGTGAACTACCAGCACCTGATGCCAACGCGCTACACCCTGGATGTGGATCTCAAAGACGTCGTCAACGTCGAGTGCTTGCAGACAAAGGACAAGAAGGTCACTGCTTTGAAGGAGGTCAAGAAGCGGTTCGAGGAGCGGTTCAAGACCGGGAAGAACAGGTGGTTCTTCACCAAGCTCAGGTTCTGA

Protein Analysis

136

Amino Acids

15.81

Weight (kDa)

10.4

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27e PF01777 52 - 135 1.3e-33 Ribosomal L27e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 317
AatII GACGTC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 358
AccII CGCG 3 cut(s) 81, 102, 250
AciI CCGC 8 cut(s) 29, 49, 100, 131, 163, 174, 346, 358
AclWI GGATC 1 cut(s) 276
AcoI YGGCCR 1 cut(s) 186
AcuI CTGAAG 1 cut(s) 35
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 3 cut(s) 154, 265, 399
AgsI TTSAA 2 cut(s) 331, 364
AjnI CCWGG 2 cut(s) 42, 258
AjuI GAANNNNNNNTTGG 1 cut(s) 386
AluBI AGCT 2 cut(s) 220, 397
AluI AGCT 2 cut(s) 220, 397
AlwI GGATC 1 cut(s) 276
AlwNI CAGNNNCTG 2 cut(s) 238, 405
AoxI GGCC 4 cut(s) 46, 66, 186, 207
ArsI GACNNNNNNTTYG 2 cut(s) 267, 299
Asp700I GAANNNNTTC 2 cut(s) 209, 347
AspLEI GCGC 4 cut(s) 63, 81, 83, 252
AspS9I GGNCC 1 cut(s) 46
AsuC2I CCSGG 2 cut(s) 58, 370
AsuHPI GGTGA 3 cut(s) 16, 43, 382
BaeGI GKGCMC 2 cut(s) 98, 120
BanI GGYRCC 1 cut(s) 95
BauI CACGAG 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 188
BceAI ACGGC 2 cut(s) 53, 201
BciT130I CCWGG 2 cut(s) 44, 260
BclI TGATCA 1 cut(s) 33
BcnI CCSGG 2 cut(s) 58, 370
BfaI CTAG 1 cut(s) 198
BisI GCNGC 1 cut(s) 49
BlsI GCNGC 1 cut(s) 50
Bme1390I CCNGG 4 cut(s) 44, 58, 260, 370
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 4 cut(s) 44, 58, 260, 370
BmsI GCATC 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 398
BpuMI CCSGG 2 cut(s) 58, 370
BsaBI GATNNNNATC 1 cut(s) 267
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 43, 153, 258
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 265, 399
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 2 cut(s) 44, 260
BseDI CCNNGG 3 cut(s) 43, 153, 258
BseGI GGATG 2 cut(s) 159, 268
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 3 cut(s) 154, 265, 399
BsePI GCGCGC 1 cut(s) 79
BseRI GAGGAG 1 cut(s) 368
BseSI GKGCMC 2 cut(s) 98, 120
BseYI CCCAGC 1 cut(s) 148
Bsh1236I CGCG 3 cut(s) 81, 102, 250
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 4 cut(s) 48, 68, 188, 209
BshNI GGYRCC 1 cut(s) 95
BsiEI CGRYCG 1 cut(s) 106
BsiSI CCGG 2 cut(s) 57, 369
BslFI GGGAC 1 cut(s) 93
BslI CCNNNNNNNGG 3 cut(s) 154, 265, 399
BsmFI GGGAC 1 cut(s) 93
BsnI GGCC 4 cut(s) 48, 68, 188, 209
Bsp1286I GDGCHC 2 cut(s) 98, 120
Bsp143I GATC 2 cut(s) 33, 268
BspACI CCGC 8 cut(s) 29, 49, 100, 131, 163, 174, 346, 358
BspANI GGCC 4 cut(s) 48, 68, 188, 209
BspFNI CGCG 3 cut(s) 81, 102, 250
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 1 cut(s) 276
BspT107I GGYRCC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 358
BssECI CCNNGG 3 cut(s) 43, 153, 258
BssHII GCGCGC 1 cut(s) 79
BssMI GATC 2 cut(s) 33, 268
BssNI GRCGYC 1 cut(s) 279
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 153
Bst2BI CACGAG 1 cut(s) 221
Bst2UI CCWGG 2 cut(s) 44, 260
Bst4CI ACNGT 2 cut(s) 107, 122
BstACI GRCGYC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 241
BstC8I GCNNGC 2 cut(s) 81, 299
BstDEI CTNAG 1 cut(s) 398
BstF5I GGATG 2 cut(s) 159, 268
BstFNI CGCG 3 cut(s) 81, 102, 250
BstHHI GCGC 4 cut(s) 63, 81, 83, 252
BstKTI GATC 2 cut(s) 36, 271
BstMBI GATC 2 cut(s) 33, 268
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 1 cut(s) 241
BstNI CCWGG 2 cut(s) 44, 260
BstSCI CCNGG 4 cut(s) 42, 56, 258, 368
BstSLI GKGCMC 2 cut(s) 98, 120
BstUI CGCG 3 cut(s) 81, 102, 250
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BsuRI GGCC 4 cut(s) 48, 68, 188, 209
BtsCI GGATG 2 cut(s) 159, 268
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 2 cut(s) 118, 321
Cac8I GCNNGC 2 cut(s) 81, 299
CaiI CAGNNNCTG 2 cut(s) 238, 405
CfoI GCGC 4 cut(s) 63, 81, 83, 252
Cfr13I GGNCC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 145
CviJI RGCY 8 cut(s) 19, 48, 68, 152, 188, 209, 220, 397
CviKI_1 RGCY 8 cut(s) 19, 48, 68, 152, 188, 209, 220, 397
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 1 cut(s) 398
DpnI GATC 2 cut(s) 35, 270
DpnII GATC 2 cut(s) 33, 268
DrdI GACNNNNNNGTC 1 cut(s) 317
DseDI GACNNNNNNGTC 1 cut(s) 317
EaeI YGGCCR 1 cut(s) 186
EciI GGCGGA 1 cut(s) 163
Eco130I CCWWGG 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 35
EcoRII CCWGG 2 cut(s) 42, 258
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
FaqI GGGAC 1 cut(s) 93
FauI CCCGC 2 cut(s) 107, 124
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 1 cut(s) 49
FokI GGATG 2 cut(s) 146, 275
Fsp4HI GCNGC 1 cut(s) 49
FspBI CTAG 1 cut(s) 198
GlaI GCGC 4 cut(s) 62, 80, 82, 251
GluI GCNGC 1 cut(s) 49
GsaI CCCAGC 1 cut(s) 152
GsuI CTGGAG 1 cut(s) 26
HaeIII GGCC 4 cut(s) 48, 68, 188, 209
HapII CCGG 2 cut(s) 57, 369
HhaI GCGC 4 cut(s) 63, 81, 83, 252
Hin1I GRCGYC 1 cut(s) 279
Hin6I GCGC 4 cut(s) 61, 79, 81, 250
HinP1I GCGC 4 cut(s) 61, 79, 81, 250
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 1 cut(s) 170
HpaII CCGG 2 cut(s) 57, 369
HphI GGTGA 3 cut(s) 16, 43, 382
Hpy166II GTNNAC 2 cut(s) 226, 286
Hpy188I TCNGA 2 cut(s) 15, 407
Hpy188III TCNNGA 4 cut(s) 198, 223, 340, 364
Hpy8I GTNNAC 2 cut(s) 226, 286
Hpy99I CGWCG 3 cut(s) 92, 284, 293
HpyAV CCTTC 5 cut(s) 94, 199, 220, 310, 325
HpyCH4III ACNGT 2 cut(s) 107, 122
HpyCH4IV ACGT 2 cut(s) 279, 288
HpyCH4V TGCA 1 cut(s) 301
HpyF10VI GCNNNNNNNGC 1 cut(s) 241
HpyF3I CTNAG 1 cut(s) 398
HpySE526I ACGT 2 cut(s) 279, 288
Hsp92I GRCGYC 1 cut(s) 279
HspAI GCGC 4 cut(s) 61, 79, 81, 250
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 2 cut(s) 33, 268
LmnI GCTCC 1 cut(s) 355
LweI GCATC 1 cut(s) 231
MaeI CTAG 1 cut(s) 198
MaeII ACGT 2 cut(s) 279, 288
MaeIII GTNAC 1 cut(s) 319
MalI GATC 2 cut(s) 35, 270
MbiI CCGCTC 1 cut(s) 358
MboI GATC 2 cut(s) 33, 268
MboII GAAGA 3 cut(s) 193, 379, 385
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 2 cut(s) 98, 120
MlyI GAGTC 1 cut(s) 164
MnlI CCTC 4 cut(s) 50, 85, 328, 346
MroXI GAANNNNTTC 2 cut(s) 209, 347
MseI TTAA 1 cut(s) 138
MspI CCGG 2 cut(s) 57, 369
MspR9I CCNGG 4 cut(s) 44, 58, 260, 370
MvaI CCWGG 2 cut(s) 44, 260
MvnI CGCG 3 cut(s) 81, 102, 250
MwoI GCNNNNNNNGC 1 cut(s) 241
NciI CCSGG 2 cut(s) 58, 370
NdeII GATC 2 cut(s) 33, 268
NlaIV GGNNCC 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 319
PauI GCGCGC 1 cut(s) 79
PceI AGGCCT 1 cut(s) 209
PcsI WCGNNNNNNNCGW 2 cut(s) 285, 288
PdmI GAANNNNTTC 2 cut(s) 209, 347
PflFI GACNNNGTC 1 cut(s) 281
PkrI GCNGC 1 cut(s) 50
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
Psp6I CCWGG 2 cut(s) 42, 258
PspFI CCCAGC 1 cut(s) 148
PspGI CCWGG 2 cut(s) 42, 258
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 1 cut(s) 46
PstNI CAGNNNCTG 2 cut(s) 238, 405
PsuI RGATCY 1 cut(s) 268
PsyI GACNNNGTC 1 cut(s) 281
PteI GCGCGC 1 cut(s) 79
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 1 cut(s) 49
Sau3AI GATC 2 cut(s) 33, 268
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 4 cut(s) 44, 58, 260, 370
SduI GDGCHC 2 cut(s) 98, 120
SfaNI GCATC 1 cut(s) 231
SseBI AGGCCT 1 cut(s) 209
SsiI CCGC 8 cut(s) 29, 49, 100, 131, 163, 174, 346, 358
SspMI CTAG 1 cut(s) 198
StuI AGGCCT 1 cut(s) 209
StyD4I CCNGG 4 cut(s) 42, 56, 258, 368
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 2 cut(s) 107, 122
TaiI ACGT 2 cut(s) 282, 291
TaqI TCGA 3 cut(s) 87, 291, 351
TauI GCSGC 1 cut(s) 51
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 2 cut(s) 125, 328
TseFI GTSAC 1 cut(s) 319
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 1 cut(s) 202
TspRI CASTG 2 cut(s) 125, 328
Tth111I GACNNNGTC 1 cut(s) 281
XbaI TCTAGA 1 cut(s) 197
XmnI GAANNNNTTC 2 cut(s) 209, 347
XspI CTAG 1 cut(s) 198
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.