FvH4_5g11610

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003801
Physical Location & Seq
Forward (+)
6589450 .. 6590361
912 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g11610.t1

Sequence Viewer

Length: 528 bp
ATGAATGGATACCTCGACAAGAAAACCCAAGGAACCGATTCCCAAAATACGAGTGTATATATTGTGACTTCCGAGCTTGAGAGCTTCGAGAGATTTAAGCGAGAGAGAAAGAAGTGCAGCGCCGCCATCACCATGATGAAGTTCTTGAAACCCAACAAGGCCGTGATCCTTCTCCAAGGTAGCTATGTCGGCCGCAAGGGCGTCATCGTCAAGGCCTTCGATGACGGCAGTCGCGACCGCGCCTACGGTCACTGCTTGGTCGCCGACATCAAGAAGTACCCCAGCAAGGTGATCCGAAAGGACTCGGCGAAGAAGACGGCGAAGAAGTCAAGGGTGAAGCTCGTGAACTACCAGCATCTAATGCCCACACGCTACACTCTGGACGTGGATCTCAAGGAGGTCGTCACCCCTGACGTGCTCACCAGCAAGGACAAGAAGGTCACCGCCTTGAAGGAGACCAAGAAGAGGTTCGAGGAGAGGTTCAAGACCGGGAAGAACAGGTGGTTCTTCACCAAGCTCAAGTTCTGA

Protein Analysis

176

Amino Acids

20.27

Weight (kDa)

10.07

Isoelectric Point (pI)

17.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27e PF01777 96 - 175 4.9e-27 Ribosomal L27e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 437
AccII CGCG 2 cut(s) 234, 240
AciI CCGC 4 cut(s) 123, 193, 238, 444
AclWI GGATC 3 cut(s) 160, 286, 396
AcoI YGGCCR 1 cut(s) 190
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 2 cut(s) 286, 465
AgsI TTSAA 3 cut(s) 148, 451, 484
AjiI CACGTC 2 cut(s) 385, 415
AjuI GAANNNNNNNTTGG 3 cut(s) 452, 484, 506
AluBI AGCT 5 cut(s) 76, 84, 183, 340, 517
AluI AGCT 5 cut(s) 76, 84, 183, 340, 517
Alw21I GWGCWC 1 cut(s) 420
Alw26I GTCTC 1 cut(s) 449
AlwI GGATC 3 cut(s) 160, 286, 396
AoxI GGCC 3 cut(s) 159, 190, 213
ApeKI GCWGC 1 cut(s) 117
Asp700I GAANNNNTTC 2 cut(s) 37, 467
AspLEI GCGC 2 cut(s) 122, 242
AsuC2I CCSGG 1 cut(s) 490
AsuHPI GGTGA 7 cut(s) 121, 301, 346, 397, 412, 433, 502
BauI CACGAG 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 420
BbvI GCAGC 1 cut(s) 129
BccI CCATC 1 cut(s) 134
BceAI ACGGC 3 cut(s) 146, 241, 333
BcnI CCSGG 1 cut(s) 490
BcoDI GTCTC 1 cut(s) 449
BfoI RGCGCY 1 cut(s) 123
BglI GCCNNNNNGGC 1 cut(s) 198
BisI GCNGC 3 cut(s) 118, 123, 193
BlsI GCNGC 3 cut(s) 119, 124, 194
Bme1390I CCNGG 1 cut(s) 490
BmgBI CACGTC 2 cut(s) 385, 415
BmiI GGNNCC 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 490
BmsI GCATC 1 cut(s) 364
BoxI GACNNNNGTC 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 320
BpuEI CTTGAG 3 cut(s) 98, 377, 503
BpuMI CCSGG 1 cut(s) 490
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 2 cut(s) 28, 175
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 465
BseDI CCNNGG 2 cut(s) 28, 175
BseLI CCNNNNNNNGG 2 cut(s) 286, 465
BseRI GAGGAG 1 cut(s) 488
BseX3I CGGCCG 1 cut(s) 190
BseXI GCAGC 1 cut(s) 129
BseYI CCCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 136
Bsh1236I CGCG 2 cut(s) 234, 240
Bsh1285I CGRYCG 2 cut(s) 193, 238
BshFI GGCC 3 cut(s) 161, 192, 215
BsiEI CGRYCG 2 cut(s) 193, 238
BsiHKAI GWGCWC 1 cut(s) 420
BsiSI CCGG 1 cut(s) 489
BslI CCNNNNNNNGG 2 cut(s) 286, 465
BsmAI GTCTC 1 cut(s) 449
BsnI GGCC 3 cut(s) 161, 192, 215
Bso31I GGTCTC 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 420
Bsp143I GATC 3 cut(s) 165, 291, 388
Bsp68I TCGCGA 1 cut(s) 234
BspACI CCGC 4 cut(s) 123, 193, 238, 444
BspANI GGCC 3 cut(s) 161, 192, 215
BspFNI CGCG 2 cut(s) 234, 240
BspLI GGNNCC 1 cut(s) 34
BspPI GGATC 3 cut(s) 160, 286, 396
BspTNI GGTCTC 1 cut(s) 449
BssECI CCNNGG 2 cut(s) 28, 175
BssMI GATC 3 cut(s) 165, 291, 388
BssNI GRCGYC 1 cut(s) 201
BssSI CACGAG 1 cut(s) 341
BssT1I CCWWGG 2 cut(s) 28, 175
Bst2BI CACGAG 1 cut(s) 341
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 1 cut(s) 458
BstACI GRCGYC 1 cut(s) 201
BstAPI GCANNNNNTGC 1 cut(s) 361
BstEII GGTNACC 1 cut(s) 439
BstFNI CGCG 2 cut(s) 234, 240
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 2 cut(s) 122, 242
BstKTI GATC 3 cut(s) 168, 294, 391
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 3 cut(s) 165, 291, 388
BstMCI CGRYCG 2 cut(s) 193, 238
BstMWI GCNNNNNNNGC 3 cut(s) 189, 198, 361
BstPAI GACNNNNGTC 1 cut(s) 228
BstPI GGTNACC 1 cut(s) 439
BstSCI CCNGG 1 cut(s) 488
BstUI CGCG 2 cut(s) 234, 240
BstV1I GCAGC 1 cut(s) 129
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BstZI CGGCCG 1 cut(s) 190
BsuRI GGCC 3 cut(s) 161, 192, 215
BtrI CACGTC 2 cut(s) 385, 415
BtsI GCAGTG 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 250
BtuMI TCGCGA 1 cut(s) 234
CfoI GCGC 2 cut(s) 122, 242
CseI GACGC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 277
CviAII CATG 1 cut(s) 133
CviJI RGCY 8 cut(s) 76, 84, 161, 183, 192, 215, 340, 517
CviKI_1 RGCY 8 cut(s) 76, 84, 161, 183, 192, 215, 340, 517
CviQI GTAC 1 cut(s) 277
DpnI GATC 3 cut(s) 167, 293, 390
DpnII GATC 3 cut(s) 165, 291, 388
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
EaeI YGGCCR 1 cut(s) 190
EagI CGGCCG 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 458
EarI CTCTTC 1 cut(s) 458
EclXI CGGCCG 1 cut(s) 190
Eco130I CCWWGG 2 cut(s) 28, 175
Eco147I AGGCCT 1 cut(s) 215
Eco31I GGTCTC 1 cut(s) 449
Eco52I CGGCCG 1 cut(s) 190
Eco91I GGTNACC 1 cut(s) 439
EcoO65I GGTNACC 1 cut(s) 439
EcoT14I CCWWGG 2 cut(s) 28, 175
ErhI CCWWGG 2 cut(s) 28, 175
FaeI CATG 1 cut(s) 136
FaiI YATR 4 cut(s) 58, 60, 134, 186
FatI CATG 1 cut(s) 132
Fnu4HI GCNGC 3 cut(s) 118, 123, 193
Fsp4HI GCNGC 3 cut(s) 118, 123, 193
GlaI GCGC 2 cut(s) 121, 241
GluI GCNGC 3 cut(s) 118, 123, 193
GsaI CCCAGC 1 cut(s) 285
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 3 cut(s) 161, 192, 215
HapII CCGG 1 cut(s) 489
HgaI GACGC 1 cut(s) 190
HhaI GCGC 2 cut(s) 122, 242
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 1 cut(s) 136
Hin6I GCGC 2 cut(s) 120, 240
HinP1I GCGC 2 cut(s) 120, 240
HinfI GANTC 2 cut(s) 38, 302
HpaII CCGG 1 cut(s) 489
HphI GGTGA 7 cut(s) 121, 301, 346, 397, 412, 433, 502
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 3 cut(s) 73, 296, 527
Hpy188III TCNNGA 7 cut(s) 88, 145, 233, 271, 343, 380, 484
Hpy8I GTNNAC 1 cut(s) 346
HpyAV CCTTC 4 cut(s) 179, 226, 430, 445
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4IV ACGT 2 cut(s) 384, 414
HpyCH4V TGCA 1 cut(s) 117
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 198, 361
HpySE526I ACGT 2 cut(s) 384, 414
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 1 cut(s) 136
HspAI GCGC 2 cut(s) 120, 240
Kzo9I GATC 3 cut(s) 165, 291, 388
LpnPI CCDG 7 cut(s) 295, 365, 365, 423, 436, 484, 502
Lsp1109I GCAGC 1 cut(s) 129
LweI GCATC 1 cut(s) 364
MaeII ACGT 2 cut(s) 384, 414
MaeIII GTNAC 4 cut(s) 64, 248, 403, 439
MalI GATC 3 cut(s) 167, 293, 390
MboI GATC 3 cut(s) 165, 291, 388
MboII GAAGA 6 cut(s) 322, 325, 334, 475, 499, 505
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 420
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 5 cut(s) 23, 391, 459, 466, 471
MroXI GAANNNNTTC 2 cut(s) 37, 467
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 2 cut(s) 131, 134
MspI CCGG 1 cut(s) 489
MspR9I CCNGG 1 cut(s) 490
MvnI CGCG 2 cut(s) 234, 240
MwoI GCNNNNNNNGC 3 cut(s) 189, 198, 361
NciI CCSGG 1 cut(s) 490
NdeII GATC 3 cut(s) 165, 291, 388
NlaIII CATG 1 cut(s) 136
NlaIV GGNNCC 1 cut(s) 34
NmeAIII GCCGAG 1 cut(s) 284
NmuCI GTSAC 4 cut(s) 64, 248, 403, 439
NruI TCGCGA 1 cut(s) 234
PceI AGGCCT 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PdmI GAANNNNTTC 2 cut(s) 37, 467
PfeI GAWTC 1 cut(s) 38
PkrI GCNGC 3 cut(s) 119, 124, 194
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
PshAI GACNNNNGTC 1 cut(s) 228
PspEI GGTNACC 1 cut(s) 439
PspFI CCCAGC 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 34
PsuI RGATCY 1 cut(s) 388
RruI TCGCGA 1 cut(s) 234
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
RseI CAYNNNNRTG 2 cut(s) 131, 134
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 3 cut(s) 118, 123, 193
Sau3AI GATC 3 cut(s) 165, 291, 388
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 490
SduI GDGCHC 1 cut(s) 420
SfaNI GCATC 1 cut(s) 364
SmiMI CAYNNNNRTG 2 cut(s) 131, 134
SmlI CTYRAG 3 cut(s) 77, 392, 518
SmoI CTYRAG 3 cut(s) 77, 392, 518
SseBI AGGCCT 1 cut(s) 215
SsiI CCGC 4 cut(s) 123, 193, 238, 444
StuI AGGCCT 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 488
StyI CCWWGG 2 cut(s) 28, 175
TaaI ACNGT 1 cut(s) 248
TaiI ACGT 2 cut(s) 387, 417
TaqI TCGA 4 cut(s) 15, 87, 219, 471
TauI GCSGC 2 cut(s) 125, 195
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 1 cut(s) 257
TseFI GTSAC 4 cut(s) 64, 248, 403, 439
TseI GCWGC 1 cut(s) 117
Tsp45I GTSAC 4 cut(s) 64, 248, 403, 439
TspDTI ATGAA 2 cut(s) 17, 152
TspRI CASTG 1 cut(s) 257
XmnI GAANNNNTTC 2 cut(s) 37, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.