Rw2G041450

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003801
Physical Location & Seq
Reverse (-)
67455829 .. 67456604
776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G041450.1

Sequence Viewer

Length: 408 bp
ATGGTGAAGTTTTTGAAGCCAAACAAGGCCGTGATCCTCCTCCAGGGCCGCTACGCCGGGCGCAAGGGCGTCATCGTCAAGGCCTTCGACGACGGCACTCGCGACCGTGCCTACGGCCACTGCCTGGTCGCCGGAATCAAGAAGTACCCGAGCAAGGTGATCCGCAAGGACTCGGCCAAGAAGACCGCCAAGAAGTCGAGGGTGAAGCCCTTCGTCAAGCTCGTCAACTACCAGCATCTGATGCCCACGCGCTACACCCTCGACGTGGATCTCAAGGAGGTCGTCACCCCCGACGTGCTCTCCAGCAAGGACAAGAAGGTCACGGCTTTGAAGGAGACCAAGAAGAGGTTCGAGGAGAGGTTCAAGACCGGAAAGAACAGGTGGTTCTTCACCAAGCTCAGGTTCTGA

Protein Analysis

135

Amino Acids

15.68

Weight (kDa)

10.42

Isoelectric Point (pI)

23.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27e PF01777 52 - 135 1e-34 Ribosomal L27e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 317
AccB7I CCANNNNNTGG 1 cut(s) 124
AccII CGCG 2 cut(s) 102, 250
AciI CCGC 3 cut(s) 49, 163, 186
AclWI GGATC 3 cut(s) 28, 154, 276
AcoI YGGCCR 2 cut(s) 115, 174
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 6 cut(s) 43, 124, 154, 265, 345, 399
AgsI TTSAA 3 cut(s) 16, 331, 364
AjiI CACGTC 2 cut(s) 265, 295
AjnI CCWGG 2 cut(s) 42, 123
AjuI GAANNNNNNNTTGG 3 cut(s) 332, 364, 386
AluBI AGCT 2 cut(s) 220, 397
AluI AGCT 2 cut(s) 220, 397
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 1 cut(s) 329
AlwI GGATC 3 cut(s) 28, 154, 276
AlwNI CAGNNNCTG 2 cut(s) 238, 405
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 5 cut(s) 27, 46, 81, 115, 174
Asp700I GAANNNNTTC 2 cut(s) 209, 347
AspLEI GCGC 2 cut(s) 63, 252
AspS9I GGNCC 1 cut(s) 46
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 5 cut(s) 16, 169, 214, 277, 382
AvaI CYCGRG 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 188
Bbv12I GWGCWC 1 cut(s) 300
BceAI ACGGC 4 cut(s) 14, 109, 130, 339
BciT130I CCWGG 2 cut(s) 44, 125
BcnI CCSGG 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 329
BisI GCNGC 1 cut(s) 49
BlsI GCNGC 1 cut(s) 50
Bme1390I CCNGG 3 cut(s) 44, 58, 125
BmeT110I CYCGRG 1 cut(s) 148
BmgBI CACGTC 2 cut(s) 265, 295
BmgT120I GGNCC 1 cut(s) 46
BmrFI CCNGG 3 cut(s) 44, 58, 125
BmsI GCATC 2 cut(s) 231, 244
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 2 cut(s) 26, 286
Bpu10I CCTNAGC 1 cut(s) 398
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 58
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 43
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 6 cut(s) 43, 124, 154, 265, 345, 399
BseBI CCWGG 2 cut(s) 44, 125
BseDI CCNNGG 1 cut(s) 43
BseLI CCNNNNNNNGG 6 cut(s) 43, 124, 154, 265, 345, 399
BseRI GAGGAG 2 cut(s) 29, 368
Bsh1236I CGCG 2 cut(s) 102, 250
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 5 cut(s) 29, 48, 83, 117, 176
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 3 cut(s) 57, 132, 369
BslI CCNNNNNNNGG 6 cut(s) 43, 124, 154, 265, 345, 399
BsmAI GTCTC 1 cut(s) 329
BsnI GGCC 5 cut(s) 29, 48, 83, 117, 176
Bso31I GGTCTC 1 cut(s) 329
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 3 cut(s) 33, 159, 268
Bsp68I TCGCGA 1 cut(s) 102
BspACI CCGC 3 cut(s) 49, 163, 186
BspANI GGCC 5 cut(s) 29, 48, 83, 117, 176
BspFNI CGCG 2 cut(s) 102, 250
BspPI GGATC 3 cut(s) 28, 154, 276
BspTNI GGTCTC 1 cut(s) 329
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 3 cut(s) 33, 159, 268
BssNI GRCGYC 1 cut(s) 69
Bst2UI CCWGG 2 cut(s) 44, 125
Bst4CI ACNGT 1 cut(s) 107
Bst6I CTCTTC 1 cut(s) 338
BstACI GRCGYC 1 cut(s) 69
BstAPI GCANNNNNTGC 1 cut(s) 241
BstDEI CTNAG 1 cut(s) 398
BstENI CCTNNNNNAGG 1 cut(s) 41
BstFNI CGCG 2 cut(s) 102, 250
BstHHI GCGC 2 cut(s) 63, 252
BstKTI GATC 3 cut(s) 36, 162, 271
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 3 cut(s) 33, 159, 268
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 1 cut(s) 241
BstNI CCWGG 2 cut(s) 44, 125
BstSCI CCNGG 3 cut(s) 42, 56, 123
BstUI CGCG 2 cut(s) 102, 250
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BsuRI GGCC 5 cut(s) 29, 48, 83, 117, 176
BtrI CACGTC 2 cut(s) 265, 295
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 118
BtuMI TCGCGA 1 cut(s) 102
CaiI CAGNNNCTG 2 cut(s) 238, 405
CfoI GCGC 2 cut(s) 63, 252
Cfr13I GGNCC 1 cut(s) 46
CseI GACGC 1 cut(s) 58
Csp6I GTAC 1 cut(s) 145
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 1 cut(s) 398
DpnI GATC 3 cut(s) 35, 161, 270
DpnII GATC 3 cut(s) 33, 159, 268
DrdI GACNNNNNNGTC 1 cut(s) 317
DseDI GACNNNNNNGTC 1 cut(s) 317
EaeI YGGCCR 2 cut(s) 115, 174
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco147I AGGCCT 1 cut(s) 83
Eco31I GGTCTC 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoRII CCWGG 2 cut(s) 42, 123
Fnu4HI GCNGC 1 cut(s) 49
Fsp4HI GCNGC 1 cut(s) 49
GlaI GCGC 2 cut(s) 62, 251
GluI GCNGC 1 cut(s) 49
GsuI CTGGAG 2 cut(s) 26, 286
HaeIII GGCC 5 cut(s) 29, 48, 83, 117, 176
HapII CCGG 3 cut(s) 57, 132, 369
HgaI GACGC 1 cut(s) 58
HhaI GCGC 2 cut(s) 63, 252
Hin1I GRCGYC 1 cut(s) 69
Hin6I GCGC 2 cut(s) 61, 250
HinP1I GCGC 2 cut(s) 61, 250
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 2 cut(s) 135, 170
HpaII CCGG 3 cut(s) 57, 132, 369
HphI GGTGA 5 cut(s) 16, 169, 214, 277, 382
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 2 cut(s) 240, 407
Hpy188III TCNNGA 3 cut(s) 101, 139, 364
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 4 cut(s) 92, 95, 266, 296
HpyAV CCTTC 4 cut(s) 94, 220, 310, 325
HpyCH4III ACNGT 1 cut(s) 107
HpyCH4IV ACGT 2 cut(s) 264, 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 241
HpyF3I CTNAG 1 cut(s) 398
HpySE526I ACGT 2 cut(s) 264, 294
Hsp92I GRCGYC 1 cut(s) 69
HspAI GCGC 2 cut(s) 61, 250
Kzo9I GATC 3 cut(s) 33, 159, 268
LweI GCATC 2 cut(s) 231, 244
MaeII ACGT 2 cut(s) 264, 294
MaeIII GTNAC 2 cut(s) 283, 319
MalI GATC 3 cut(s) 35, 161, 270
MboI GATC 3 cut(s) 33, 159, 268
MboII GAAGA 3 cut(s) 193, 355, 379
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 300
MlyI GAGTC 1 cut(s) 164
MnlI CCTC 8 cut(s) 47, 50, 192, 269, 271, 339, 346, 351
MroXI GAANNNNTTC 2 cut(s) 209, 347
MspI CCGG 3 cut(s) 57, 132, 369
MspR9I CCNGG 3 cut(s) 44, 58, 125
MvaI CCWGG 2 cut(s) 44, 125
MvnI CGCG 2 cut(s) 102, 250
MwoI GCNNNNNNNGC 1 cut(s) 241
NciI CCSGG 1 cut(s) 58
NdeII GATC 3 cut(s) 33, 159, 268
NmeAIII GCCGAG 1 cut(s) 152
NmuCI GTSAC 2 cut(s) 283, 319
NruI TCGCGA 1 cut(s) 102
PceI AGGCCT 1 cut(s) 83
PcsI WCGNNNNNNNCGW 3 cut(s) 99, 219, 288
PdmI GAANNNNTTC 2 cut(s) 209, 347
PfeI GAWTC 1 cut(s) 135
PflMI CCANNNNNTGG 1 cut(s) 124
PkrI GCNGC 1 cut(s) 50
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
Psp6I CCWGG 2 cut(s) 42, 123
PspGI CCWGG 2 cut(s) 42, 123
PspPI GGNCC 1 cut(s) 46
PstNI CAGNNNCTG 2 cut(s) 238, 405
PsuI RGATCY 1 cut(s) 268
RruI TCGCGA 1 cut(s) 102
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
SatI GCNGC 1 cut(s) 49
Sau3AI GATC 3 cut(s) 33, 159, 268
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 3 cut(s) 44, 58, 125
SduI GDGCHC 1 cut(s) 300
SfaNI GCATC 2 cut(s) 231, 244
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 3 cut(s) 49, 163, 186
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 3 cut(s) 42, 56, 123
TaaI ACNGT 1 cut(s) 107
TaiI ACGT 2 cut(s) 267, 297
TaqI TCGA 4 cut(s) 87, 197, 261, 351
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 1 cut(s) 135
TscAI CASTG 1 cut(s) 125
TseFI GTSAC 2 cut(s) 283, 319
Tsp45I GTSAC 2 cut(s) 283, 319
TspRI CASTG 1 cut(s) 125
Van91I CCANNNNNTGG 1 cut(s) 124
XagI CCTNNNNNAGG 1 cut(s) 41
XmnI GAANNNNTTC 2 cut(s) 209, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.