Rh5BG145700

Belongs to the eukaryotic ribosomal protein eL27 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003801
Physical Location & Seq
Forward (+)
15058830 .. 15059225
396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG145700.1

Sequence Viewer

Length: 396 bp
ATGGTGAAATTTCTGAAGCCAAACAAAGCAGTGCTCCTACTTCAGGGGCGGTTTGCCGGAAGGAAGGCCGTAATCTTGAAGGCCTTCGACGAAGGGACACGTGACCGGCCGTACGGGCACTGCTTGGTGGCCGGAATAGCCAAGTACCCGAGCAAGGTGATCCGGAAGGACTCGGCCAAGAAGTCGAGGGTCAAGGCCTTCATCAAGCTCGTCAACTACCAGCACGTGATGCCCACGCGCTACAGCCTCGACGTCGACCTCAAGGAGGTGGCCACCGTCGACGCGTTGCACTCACGCGACAAGAAGGTCACGGCCGCCAAGGAGATCAAGGCCAGATTGGAGGACCGGTTCAAGAGTGGCAAGAACCGGTGGTTCTTTACCAAGCTTAGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
KOW Ribosomal_L27e

Protein Analysis

131

Amino Acids

15.08

Weight (kDa)

10.45

Isoelectric Point (pI)

22.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27e PF01777 55 - 131 2.2e-29 Ribosomal L27e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 305
AatII GACGTC 1 cut(s) 255
AccI GTMKAC 2 cut(s) 255, 279
AccII CGCG 3 cut(s) 238, 284, 297
AccIII TCCGGA 1 cut(s) 162
AciI CCGC 2 cut(s) 49, 315
AclWI GGATC 1 cut(s) 154
AcoI YGGCCR 5 cut(s) 107, 129, 174, 270, 312
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 2 cut(s) 26, 35
AcvI CACGTG 2 cut(s) 101, 226
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 2 cut(s) 113, 146
AfiI CCNNNNNNNGG 4 cut(s) 43, 154, 265, 387
AflIII ACRYGT 2 cut(s) 98, 282
AgeI ACCGGT 2 cut(s) 345, 366
AgsI TTSAA 2 cut(s) 79, 352
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
AluBI AGCT 2 cut(s) 208, 385
AluI AGCT 2 cut(s) 208, 385
Alw21I GWGCWC 1 cut(s) 36
AlwI GGATC 1 cut(s) 154
Ama87I CYCGRG 1 cut(s) 148
Aor13HI TCCGGA 1 cut(s) 162
AoxI GGCC 9 cut(s) 66, 81, 107, 129, 174, 195, 270, 312, 330
ApoI RAATTY 1 cut(s) 8
AsiGI ACCGGT 2 cut(s) 345, 366
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 240
AspS9I GGNCC 1 cut(s) 343
AsuHPI GGTGA 2 cut(s) 16, 169
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 343
BaeGI GKGCMC 1 cut(s) 120
BalI TGGCCA 1 cut(s) 272
BbrPI CACGTG 2 cut(s) 101, 226
Bbv12I GWGCWC 1 cut(s) 36
BceAI ACGGC 3 cut(s) 53, 94, 327
BfmI CTRYAG 1 cut(s) 241
BglI GCCNNNNNGGC 1 cut(s) 115
BisI GCNGC 1 cut(s) 315
BlsI GCNGC 1 cut(s) 316
Bme18I GGWCC 1 cut(s) 343
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 343
BmsI GCATC 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 386
BpuEI CTTGAG 1 cut(s) 245
BsaAI YACGTR 2 cut(s) 101, 226
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 318
BsaWI WCCGGW 3 cut(s) 162, 345, 366
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 154, 265, 387
Bse118I RCCGGY 3 cut(s) 105, 345, 366
BseAI TCCGGA 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 318
BseLI CCNNNNNNNGG 4 cut(s) 43, 154, 265, 387
BseSI GKGCMC 1 cut(s) 120
BseX3I CGGCCG 2 cut(s) 107, 312
Bsh1236I CGCG 3 cut(s) 238, 284, 297
Bsh1285I CGRYCG 2 cut(s) 110, 315
BshFI GGCC 9 cut(s) 68, 83, 109, 131, 176, 197, 272, 314, 332
BshTI ACCGGT 2 cut(s) 345, 366
BsiEI CGRYCG 2 cut(s) 110, 315
BsiHKAI GWGCWC 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 6 cut(s) 57, 106, 132, 163, 346, 367
BsiWI CGTACG 1 cut(s) 111
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 4 cut(s) 43, 154, 265, 387
BsmFI GGGAC 1 cut(s) 109
BsnI GGCC 9 cut(s) 68, 83, 109, 131, 176, 197, 272, 314, 332
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 2 cut(s) 36, 120
Bsp13I TCCGGA 1 cut(s) 162
Bsp143I GATC 2 cut(s) 159, 324
BspACI CCGC 2 cut(s) 49, 315
BspANI GGCC 9 cut(s) 68, 83, 109, 131, 176, 197, 272, 314, 332
BspEI TCCGGA 1 cut(s) 162
BspFNI CGCG 3 cut(s) 238, 284, 297
BspPI GGATC 1 cut(s) 154
BsrFI RCCGGY 3 cut(s) 105, 345, 366
BssAI RCCGGY 3 cut(s) 105, 345, 366
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 2 cut(s) 159, 324
BssNI GRCGYC 1 cut(s) 252
BssT1I CCWWGG 1 cut(s) 318
Bst4CI ACNGT 1 cut(s) 277
BstACI GRCGYC 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 229
BstBAI YACGTR 2 cut(s) 101, 226
BstDEI CTNAG 1 cut(s) 386
BstENI CCTNNNNNAGG 2 cut(s) 41, 263
BstFNI CGCG 3 cut(s) 238, 284, 297
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 2 cut(s) 162, 327
BstMBI GATC 2 cut(s) 159, 324
BstMCI CGRYCG 2 cut(s) 110, 315
BstMWI GCNNNNNNNGC 3 cut(s) 115, 137, 229
BstSFI CTRYAG 1 cut(s) 241
BstSLI GKGCMC 1 cut(s) 120
BstUI CGCG 3 cut(s) 238, 284, 297
BstZI CGGCCG 2 cut(s) 107, 312
BsuRI GGCC 9 cut(s) 68, 83, 109, 131, 176, 197, 272, 314, 332
BtsI GCAGTG 2 cut(s) 36, 118
BtsIMutI CAGTG 2 cut(s) 36, 118
CfoI GCGC 1 cut(s) 240
Cfr10I RCCGGY 3 cut(s) 105, 345, 366
Cfr13I GGNCC 1 cut(s) 343
CseI GACGC 1 cut(s) 290
Csp6I GTAC 2 cut(s) 112, 145
CspAI ACCGGT 2 cut(s) 345, 366
CviQI GTAC 2 cut(s) 112, 145
DdeI CTNAG 1 cut(s) 386
DpnI GATC 2 cut(s) 161, 326
DpnII GATC 2 cut(s) 159, 324
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
EaeI YGGCCR 5 cut(s) 107, 129, 174, 270, 312
EagI CGGCCG 2 cut(s) 107, 312
EclXI CGGCCG 2 cut(s) 107, 312
Eco130I CCWWGG 1 cut(s) 318
Eco147I AGGCCT 2 cut(s) 83, 197
Eco47I GGWCC 1 cut(s) 343
Eco52I CGGCCG 2 cut(s) 107, 312
Eco57I CTGAAG 2 cut(s) 26, 35
Eco72I CACGTG 2 cut(s) 101, 226
Eco88I CYCGRG 1 cut(s) 148
EcoNI CCTNNNNNAGG 2 cut(s) 41, 263
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
FaqI GGGAC 1 cut(s) 109
FblI GTMKAC 2 cut(s) 255, 279
Fnu4HI GCNGC 1 cut(s) 315
Fsp4HI GCNGC 1 cut(s) 315
GlaI GCGC 1 cut(s) 239
GluI GCNGC 1 cut(s) 315
HaeIII GGCC 9 cut(s) 68, 83, 109, 131, 176, 197, 272, 314, 332
HapII CCGG 6 cut(s) 57, 106, 132, 163, 346, 367
HgaI GACGC 1 cut(s) 290
HhaI GCGC 1 cut(s) 240
Hin1I GRCGYC 1 cut(s) 252
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HincII GTYRAC 3 cut(s) 214, 256, 280
HindII GTYRAC 3 cut(s) 214, 256, 280
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 1 cut(s) 170
HpaII CCGG 6 cut(s) 57, 106, 132, 163, 346, 367
HphI GGTGA 2 cut(s) 16, 169
Hpy166II GTNNAC 3 cut(s) 214, 256, 280
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 3 cut(s) 76, 163, 352
Hpy8I GTNNAC 3 cut(s) 214, 256, 280
Hpy99I CGWCG 5 cut(s) 92, 254, 257, 281, 284
HpyAV CCTTC 8 cut(s) 54, 58, 73, 86, 94, 160, 208, 298
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 3 cut(s) 100, 225, 252
HpyCH4V TGCA 1 cut(s) 289
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 137, 229
HpyF3I CTNAG 1 cut(s) 386
HpySE526I ACGT 3 cut(s) 100, 225, 252
Hsp92I GRCGYC 1 cut(s) 252
HspAI GCGC 1 cut(s) 238
Kpn2I TCCGGA 1 cut(s) 162
Kzo9I GATC 2 cut(s) 159, 324
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 9 cut(s) 29, 70, 119, 145, 176, 233, 346, 359, 380
LweI GCATC 1 cut(s) 219
MaeII ACGT 3 cut(s) 100, 225, 252
MaeIII GTNAC 2 cut(s) 101, 307
MalI GATC 2 cut(s) 161, 326
MboI GATC 2 cut(s) 159, 324
MhlI GDGCHC 2 cut(s) 36, 120
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 1 cut(s) 8
MluI ACGCGT 1 cut(s) 282
MluNI TGGCCA 1 cut(s) 272
MlyI GAGTC 1 cut(s) 164
MnlI CCTC 5 cut(s) 180, 257, 259, 269, 334
Mox20I TGGCCA 1 cut(s) 272
MroI TCCGGA 1 cut(s) 162
MroXI GAANNNNTTC 1 cut(s) 83
MscI TGGCCA 1 cut(s) 272
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 6 cut(s) 57, 106, 132, 163, 346, 367
MvnI CGCG 3 cut(s) 238, 284, 297
MwoI GCNNNNNNNGC 3 cut(s) 115, 137, 229
NdeII GATC 2 cut(s) 159, 324
NmeAIII GCCGAG 1 cut(s) 152
NmuCI GTSAC 2 cut(s) 101, 307
PceI AGGCCT 2 cut(s) 83, 197
PdmI GAANNNNTTC 1 cut(s) 83
Pfl23II CGTACG 1 cut(s) 111
PinAI ACCGGT 2 cut(s) 345, 366
PkrI GCNGC 1 cut(s) 316
PleI GAGTC 1 cut(s) 164
PmaCI CACGTG 2 cut(s) 101, 226
PmlI CACGTG 2 cut(s) 101, 226
PpsI GAGTC 1 cut(s) 164
Ppu21I YACGTR 2 cut(s) 101, 226
PspCI CACGTG 2 cut(s) 101, 226
PspLI CGTACG 1 cut(s) 111
PspPI GGNCC 1 cut(s) 343
RsaI GTAC 2 cut(s) 113, 146
RsaNI GTAC 2 cut(s) 112, 145
SalI GTCGAC 2 cut(s) 254, 278
SatI GCNGC 1 cut(s) 315
Sau3AI GATC 2 cut(s) 159, 324
Sau96I GGNCC 1 cut(s) 343
SchI GAGTC 1 cut(s) 164
SduI GDGCHC 2 cut(s) 36, 120
SfaNI GCATC 1 cut(s) 219
SfcI CTRYAG 1 cut(s) 241
SgrDI CGTCGACG 1 cut(s) 278
SinI GGWCC 1 cut(s) 343
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 1 cut(s) 8
SseBI AGGCCT 2 cut(s) 83, 197
SsiI CCGC 2 cut(s) 49, 315
StuI AGGCCT 2 cut(s) 83, 197
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 3 cut(s) 103, 228, 255
TaqI TCGA 5 cut(s) 87, 185, 249, 255, 279
TasI AATT 1 cut(s) 8
TauI GCSGC 1 cut(s) 317
TscAI CASTG 2 cut(s) 36, 125
TseFI GTSAC 2 cut(s) 101, 307
Tsp45I GTSAC 2 cut(s) 101, 307
TspDTI ATGAA 1 cut(s) 190
TspRI CASTG 2 cut(s) 36, 125
VpaK11BI GGWCC 1 cut(s) 343
XagI CCTNNNNNAGG 2 cut(s) 41, 263
XapI RAATTY 1 cut(s) 8
XmiI GTMKAC 2 cut(s) 255, 279
XmnI GAANNNNTTC 1 cut(s) 83
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.