Rh2DG527200

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003801
Physical Location & Seq
Reverse (-)
75228214 .. 75229198
985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG527200.1

Sequence Viewer

Length: 234 bp
ATGCCCACGCGCTACACCCTTGACGTGGATCTCAAGGAGGTCGTCACTCCCGACGTGCTCTCCAGCAAGGACAAGAAGGTCACGGCTTTGAAGGAGACCAAGAAGAGGTTTGAGGAGAGGTTCAAGACCGGAAAGAACAGGTGGTTCTTCACCAAGCTCAGACTGACTATATTGGCCAGAGCTTTCTCATACATGAAACAGATGGACATACCTTCCTCACCAATGGTTAACTAG

Protein Analysis

77

Amino Acids

9.1

Weight (kDa)

10.06

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27e PF01777 1 - 54 9.2e-20 Ribosomal L27e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AccII CGCG 1 cut(s) 10
AclWI GGATC 1 cut(s) 36
AcoI YGGCCR 1 cut(s) 174
AfiI CCNNNNNNNGG 2 cut(s) 25, 105
AgsI TTSAA 2 cut(s) 91, 124
AjiI CACGTC 2 cut(s) 25, 55
AluBI AGCT 2 cut(s) 157, 182
AluI AGCT 2 cut(s) 157, 182
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 1 cut(s) 36
AoxI GGCC 1 cut(s) 174
AspLEI GCGC 1 cut(s) 12
AsuHPI GGTGA 2 cut(s) 142, 210
BalI TGGCCA 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 60
BccI CCATC 1 cut(s) 196
BceAI ACGGC 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 1 cut(s) 232
BmgBI CACGTC 2 cut(s) 25, 55
BpmI CTGGAG 1 cut(s) 46
BpuEI CTTGAG 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 89
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 105
BseLI CCNNNNNNNGG 2 cut(s) 25, 105
BseMII CTCAG 1 cut(s) 172
BseRI GAGGAG 1 cut(s) 128
Bsh1236I CGCG 1 cut(s) 10
BshFI GGCC 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 60
BsiSI CCGG 1 cut(s) 129
BslI CCNNNNNNNGG 2 cut(s) 25, 105
BsmAI GTCTC 1 cut(s) 89
BsnI GGCC 1 cut(s) 176
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 1 cut(s) 28
BspANI GGCC 1 cut(s) 176
BspCNI CTCAG 1 cut(s) 171
BspFNI CGCG 1 cut(s) 10
BspPI GGATC 1 cut(s) 36
BspTNI GGTCTC 1 cut(s) 89
BssMI GATC 1 cut(s) 28
Bst6I CTCTTC 1 cut(s) 98
BstDEI CTNAG 1 cut(s) 158
BstFNI CGCG 1 cut(s) 10
BstHHI GCGC 1 cut(s) 12
BstKTI GATC 1 cut(s) 31
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 1 cut(s) 28
BstUI CGCG 1 cut(s) 10
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
BsuRI GGCC 1 cut(s) 176
BtrI CACGTC 2 cut(s) 25, 55
CfoI GCGC 1 cut(s) 12
CviAII CATG 1 cut(s) 193
CviJI RGCY 4 cut(s) 86, 157, 176, 182
CviKI_1 RGCY 4 cut(s) 86, 157, 176, 182
DdeI CTNAG 1 cut(s) 158
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco31I GGTCTC 1 cut(s) 89
FaeI CATG 1 cut(s) 196
FaiI YATR 4 cut(s) 170, 190, 194, 209
FatI CATG 1 cut(s) 192
FspBI CTAG 1 cut(s) 232
GlaI GCGC 1 cut(s) 11
GsuI CTGGAG 1 cut(s) 46
HaeIII GGCC 1 cut(s) 176
HapII CCGG 1 cut(s) 129
HhaI GCGC 1 cut(s) 12
Hin1II CATG 1 cut(s) 196
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 1 cut(s) 129
HphI GGTGA 2 cut(s) 142, 210
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 1 cut(s) 161
Hpy188III TCNNGA 2 cut(s) 50, 124
Hpy8I GTNNAC 1 cut(s) 229
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 3 cut(s) 70, 85, 222
HpyCH4IV ACGT 2 cut(s) 24, 54
HpyF3I CTNAG 1 cut(s) 158
HpySE526I ACGT 2 cut(s) 24, 54
Hsp92II CATG 1 cut(s) 196
HspAI GCGC 1 cut(s) 10
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 1 cut(s) 28
LpnPI CCDG 4 cut(s) 76, 124, 142, 190
MaeI CTAG 1 cut(s) 232
MaeII ACGT 2 cut(s) 24, 54
MaeIII GTNAC 2 cut(s) 43, 79
MalI GATC 1 cut(s) 30
MboI GATC 1 cut(s) 28
MboII GAAGA 2 cut(s) 115, 139
MflI RGATCY 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 60
MlsI TGGCCA 1 cut(s) 176
MluNI TGGCCA 1 cut(s) 176
MnlI CCTC 5 cut(s) 31, 99, 106, 111, 226
Mox20I TGGCCA 1 cut(s) 176
MscI TGGCCA 1 cut(s) 176
MseI TTAA 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 176
MspI CCGG 1 cut(s) 129
MvnI CGCG 1 cut(s) 10
NdeII GATC 1 cut(s) 28
NlaIII CATG 1 cut(s) 196
NmuCI GTSAC 2 cut(s) 43, 79
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PsuI RGATCY 1 cut(s) 28
SaqAI TTAA 1 cut(s) 228
Sau3AI GATC 1 cut(s) 28
SduI GDGCHC 1 cut(s) 60
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
SspMI CTAG 1 cut(s) 232
TaiI ACGT 2 cut(s) 27, 57
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseFI GTSAC 2 cut(s) 43, 79
Tsp45I GTSAC 2 cut(s) 43, 79
TspDTI ATGAA 1 cut(s) 209
XspI CTAG 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.