Rroxscaffold_1G00058200

Belongs to the eukaryotic ribosomal protein eL27 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003801
Physical Location & Seq
Reverse (-)
80063045 .. 80063452
408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058200.1

Sequence Viewer

Length: 408 bp
ATGGTGAAATTTCTGAAGCCAAACAAAGCAGTGCTCCTACTTCAGGGGCGGTTCGCCGGAAGGAAGGCCGTAATCGTGAAGGCCTTCGACGAAGGGACACGTGACCGGCCGTACGGGCACTGCTTGGTGGCCGGAATAGCCAAGTACCCGAGCAAGGTGATCCGGAAGGACTCGGCCAAGAAGACGGCCAAGAAGTCGAGGGTCAAGGCCTTCATCAAGCTCGTCAACTACCAGCACGTGATGCCCACGCGCTACAGCCTCGACGTCGACCTCAAGGAGGTGGCCACCGTCGACGCGTTGCACTCACGCGACAAGAAGGTCACGGCCGCCAAGGAGATCAAGGCCAGATTGGAGGATCGCTTCAAGAGTGGCAAGAACCGGTGGTTCTTTACCAAGCTTAGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
KOW Ribosomal_L27e

Protein Analysis

135

Amino Acids

15.5

Weight (kDa)

10.49

Isoelectric Point (pI)

20.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27e PF01777 52 - 135 5e-32 Ribosomal L27e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 317
AatII GACGTC 1 cut(s) 267
AccI GTMKAC 2 cut(s) 267, 291
AccII CGCG 3 cut(s) 250, 296, 309
AccIII TCCGGA 1 cut(s) 162
AciI CCGC 2 cut(s) 49, 327
AclWI GGATC 2 cut(s) 154, 363
AcoI YGGCCR 6 cut(s) 107, 129, 174, 186, 282, 324
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 2 cut(s) 26, 35
AcvI CACGTG 2 cut(s) 101, 238
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 2 cut(s) 113, 146
AfiI CCNNNNNNNGG 4 cut(s) 43, 154, 277, 399
AflIII ACRYGT 2 cut(s) 98, 294
AgeI ACCGGT 1 cut(s) 378
AgsI TTSAA 1 cut(s) 364
AluBI AGCT 2 cut(s) 220, 397
AluI AGCT 2 cut(s) 220, 397
Alw21I GWGCWC 1 cut(s) 36
AlwI GGATC 2 cut(s) 154, 363
Ama87I CYCGRG 1 cut(s) 148
Aor13HI TCCGGA 1 cut(s) 162
ApoI RAATTY 1 cut(s) 8
AsiGI ACCGGT 1 cut(s) 378
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 252
AsuHPI GGTGA 2 cut(s) 16, 169
AvaI CYCGRG 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 120
BalI TGGCCA 1 cut(s) 284
BbrPI CACGTG 2 cut(s) 101, 238
BbsI GAAGAC 1 cut(s) 188
Bbv12I GWGCWC 1 cut(s) 36
BceAI ACGGC 4 cut(s) 53, 94, 201, 339
BfmI CTRYAG 1 cut(s) 253
BglI GCCNNNNNGGC 1 cut(s) 115
BisI GCNGC 1 cut(s) 327
BlsI GCNGC 1 cut(s) 328
BmeT110I CYCGRG 1 cut(s) 148
BmsI GCATC 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 398
BpuEI CTTGAG 1 cut(s) 257
BsaAI YACGTR 2 cut(s) 101, 238
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 330
BsaWI WCCGGW 2 cut(s) 162, 378
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 154, 277, 399
Bse118I RCCGGY 2 cut(s) 105, 378
BseAI TCCGGA 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 330
BseLI CCNNNNNNNGG 4 cut(s) 43, 154, 277, 399
BseSI GKGCMC 1 cut(s) 120
BseX3I CGGCCG 2 cut(s) 107, 324
Bsh1236I CGCG 3 cut(s) 250, 296, 309
Bsh1285I CGRYCG 2 cut(s) 110, 327
BshTI ACCGGT 1 cut(s) 378
BsiEI CGRYCG 2 cut(s) 110, 327
BsiHKAI GWGCWC 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 5 cut(s) 57, 106, 132, 163, 379
BsiWI CGTACG 1 cut(s) 111
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 4 cut(s) 43, 154, 277, 399
BsmFI GGGAC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 2 cut(s) 36, 120
Bsp13I TCCGGA 1 cut(s) 162
Bsp143I GATC 3 cut(s) 159, 336, 355
BspACI CCGC 2 cut(s) 49, 327
BspEI TCCGGA 1 cut(s) 162
BspFNI CGCG 3 cut(s) 250, 296, 309
BspPI GGATC 2 cut(s) 154, 363
BsrFI RCCGGY 2 cut(s) 105, 378
BssAI RCCGGY 2 cut(s) 105, 378
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 3 cut(s) 159, 336, 355
BssNI GRCGYC 1 cut(s) 264
BssT1I CCWWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 289
BstACI GRCGYC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 241
BstBAI YACGTR 2 cut(s) 101, 238
BstDEI CTNAG 1 cut(s) 398
BstENI CCTNNNNNAGG 2 cut(s) 41, 275
BstFNI CGCG 3 cut(s) 250, 296, 309
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 3 cut(s) 162, 339, 358
BstMBI GATC 3 cut(s) 159, 336, 355
BstMCI CGRYCG 2 cut(s) 110, 327
BstMWI GCNNNNNNNGC 3 cut(s) 115, 137, 241
BstSFI CTRYAG 1 cut(s) 253
BstSLI GKGCMC 1 cut(s) 120
BstUI CGCG 3 cut(s) 250, 296, 309
BstV2I GAAGAC 1 cut(s) 188
BstZI CGGCCG 2 cut(s) 107, 324
BtsI GCAGTG 2 cut(s) 36, 118
BtsIMutI CAGTG 2 cut(s) 36, 118
CfoI GCGC 1 cut(s) 252
Cfr10I RCCGGY 2 cut(s) 105, 378
CseI GACGC 1 cut(s) 302
Csp6I GTAC 2 cut(s) 112, 145
CspAI ACCGGT 1 cut(s) 378
CviQI GTAC 2 cut(s) 112, 145
DdeI CTNAG 1 cut(s) 398
DpnI GATC 3 cut(s) 161, 338, 357
DpnII GATC 3 cut(s) 159, 336, 355
DrdI GACNNNNNNGTC 1 cut(s) 317
DseDI GACNNNNNNGTC 1 cut(s) 317
EaeI YGGCCR 6 cut(s) 107, 129, 174, 186, 282, 324
EagI CGGCCG 2 cut(s) 107, 324
EclXI CGGCCG 2 cut(s) 107, 324
Eco130I CCWWGG 1 cut(s) 330
Eco147I AGGCCT 2 cut(s) 83, 209
Eco52I CGGCCG 2 cut(s) 107, 324
Eco57I CTGAAG 2 cut(s) 26, 35
Eco72I CACGTG 2 cut(s) 101, 238
Eco88I CYCGRG 1 cut(s) 148
EcoNI CCTNNNNNAGG 2 cut(s) 41, 275
EcoT14I CCWWGG 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 330
FaqI GGGAC 1 cut(s) 109
FblI GTMKAC 2 cut(s) 267, 291
Fnu4HI GCNGC 1 cut(s) 327
Fsp4HI GCNGC 1 cut(s) 327
GlaI GCGC 1 cut(s) 251
GluI GCNGC 1 cut(s) 327
HapII CCGG 5 cut(s) 57, 106, 132, 163, 379
HgaI GACGC 1 cut(s) 302
HhaI GCGC 1 cut(s) 252
Hin1I GRCGYC 1 cut(s) 264
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HincII GTYRAC 3 cut(s) 226, 268, 292
HindII GTYRAC 3 cut(s) 226, 268, 292
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 1 cut(s) 170
HpaII CCGG 5 cut(s) 57, 106, 132, 163, 379
HphI GGTGA 2 cut(s) 16, 169
Hpy166II GTNNAC 3 cut(s) 226, 268, 292
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 3 cut(s) 76, 163, 364
Hpy8I GTNNAC 3 cut(s) 226, 268, 292
Hpy99I CGWCG 5 cut(s) 92, 266, 269, 293, 296
HpyAV CCTTC 8 cut(s) 54, 58, 73, 86, 94, 160, 220, 310
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 3 cut(s) 100, 237, 264
HpyCH4V TGCA 1 cut(s) 301
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 137, 241
HpyF3I CTNAG 1 cut(s) 398
HpySE526I ACGT 3 cut(s) 100, 237, 264
Hsp92I GRCGYC 1 cut(s) 264
HspAI GCGC 1 cut(s) 250
Kpn2I TCCGGA 1 cut(s) 162
Kzo9I GATC 3 cut(s) 159, 336, 355
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 8 cut(s) 29, 70, 119, 145, 176, 245, 358, 392
LweI GCATC 1 cut(s) 231
MaeII ACGT 3 cut(s) 100, 237, 264
MaeIII GTNAC 2 cut(s) 101, 319
MalI GATC 3 cut(s) 161, 338, 357
MboI GATC 3 cut(s) 159, 336, 355
MboII GAAGA 1 cut(s) 193
MhlI GDGCHC 2 cut(s) 36, 120
MlsI TGGCCA 1 cut(s) 284
MluCI AATT 1 cut(s) 8
MluI ACGCGT 1 cut(s) 294
MluNI TGGCCA 1 cut(s) 284
MlyI GAGTC 1 cut(s) 164
MnlI CCTC 5 cut(s) 192, 269, 271, 281, 346
Mox20I TGGCCA 1 cut(s) 284
MroI TCCGGA 1 cut(s) 162
MroXI GAANNNNTTC 1 cut(s) 83
MscI TGGCCA 1 cut(s) 284
Msp20I TGGCCA 1 cut(s) 284
MspI CCGG 5 cut(s) 57, 106, 132, 163, 379
MvnI CGCG 3 cut(s) 250, 296, 309
MwoI GCNNNNNNNGC 3 cut(s) 115, 137, 241
NdeII GATC 3 cut(s) 159, 336, 355
NmeAIII GCCGAG 1 cut(s) 152
NmuCI GTSAC 2 cut(s) 101, 319
PceI AGGCCT 2 cut(s) 83, 209
PdmI GAANNNNTTC 1 cut(s) 83
Pfl23II CGTACG 1 cut(s) 111
PinAI ACCGGT 1 cut(s) 378
PkrI GCNGC 1 cut(s) 328
PleI GAGTC 1 cut(s) 164
PmaCI CACGTG 2 cut(s) 101, 238
PmlI CACGTG 2 cut(s) 101, 238
PpsI GAGTC 1 cut(s) 164
Ppu21I YACGTR 2 cut(s) 101, 238
PspCI CACGTG 2 cut(s) 101, 238
PspLI CGTACG 1 cut(s) 111
RsaI GTAC 2 cut(s) 113, 146
RsaNI GTAC 2 cut(s) 112, 145
SalI GTCGAC 2 cut(s) 266, 290
SatI GCNGC 1 cut(s) 327
Sau3AI GATC 3 cut(s) 159, 336, 355
SchI GAGTC 1 cut(s) 164
SduI GDGCHC 2 cut(s) 36, 120
SfaNI GCATC 1 cut(s) 231
SfcI CTRYAG 1 cut(s) 253
SgrDI CGTCGACG 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 1 cut(s) 8
SseBI AGGCCT 2 cut(s) 83, 209
SsiI CCGC 2 cut(s) 49, 327
StuI AGGCCT 2 cut(s) 83, 209
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 3 cut(s) 103, 240, 267
TaqI TCGA 5 cut(s) 87, 197, 261, 267, 291
TasI AATT 1 cut(s) 8
TauI GCSGC 1 cut(s) 329
TscAI CASTG 2 cut(s) 36, 125
TseFI GTSAC 2 cut(s) 101, 319
Tsp45I GTSAC 2 cut(s) 101, 319
TspDTI ATGAA 1 cut(s) 202
TspRI CASTG 2 cut(s) 36, 125
XagI CCTNNNNNAGG 2 cut(s) 41, 275
XapI RAATTY 1 cut(s) 8
XmiI GTMKAC 2 cut(s) 267, 291
XmnI GAANNNNTTC 1 cut(s) 83
ZraI GACGTC 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.