AT2G46930

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002705
Physical Location & Seq
Forward (+)
19283456 .. 19286400
2945 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46930.1

Sequence Viewer

Length: 1251 bp
ATGAAGAGTGTGTTGCGTATTGCGGCGGCGATATTCTGGCTTTGGCTGTTTATCGTGTTAGGTGTGATTGGGAGTGGGAATGTGAGAGATACAGACGATGAGATCTCGTTACTCGAAAGTCAATTGGTGGTGACATCTCCGTCGCAGCTTCTTATGGTGCCTCTCACTTTGATTCAGGCTGCTGCCTCCAAAGGAGCTGTGTGCCTGGATGGAACACTACCTGGTTATCATCTACACCCTGGTTCTGGATCAGGAGCTAACCGGTGGCTCATCCAACTCGAGGGTGGAGGATGGTGCAACACACGTAGGAGCTGTATCTTCCGGAAAACCACTCGCCGTGGTTCATCAAATCATATGGAGAAAGTTTTGGCCTTCACTGGAATATTGAGCAATAAATCTAATGAGAATCCTGACTTCTTCAACTGGAACAGAGTCAAATTGCGTTACTGCGATGGTGCCTCTTTCACCGGCGATAGTCAGGATGAGAGCTCACAACTTTACTATAGAGGACAACGAATCTGGCATTCAGCTATGGAAGAACTACTCTCTAAAGGCATGCAAAAAGCAGAACAGGCTCTACTTTCTGGATGTTCAGCTGGGGGATTAGCTTCCATCCTACACTGCGATCAGTTCAAGGAACTATTTCCGGGCACTACGACAGTGAAATGCTTAAGTGATGCTGGAATGTTTATGGATGCAGTGGATGTCTCTGGGGGCCACTCGCTCCGGAAAATGTTCCAAGGTGTTGTTACAGTACAGAACCTCCAAAAGGAACTGTCCACTGCTTGTACAAAGCATTTGGATCCAACTTCGTGCTTCTTTCCCCAGAACTTGGTTTCAGGCATTAAGACTCCAATGTTTCTTCTCAATGCAGCATATGACGCTTGGCAGGTACAAGAGAGTTTAGCTCCACCATCAGTTGACCTAAGCGGCTCTTGGAAGGCATGCAAATCTGATCACTCGCATTGTAATTCATCTCAGATCCAGTTCTTCCAAGACTTCAGGACTCATATGGTAGATGCTGTAAAGTCTTTCGCGACATCGACACATAACGGTGTGTTCATAAACTCATGCTTCGCTCACTGCCAATCTGAAAGACAGGACACTTGGTATGCACCAGATTCTCCTACTCTTCATGGCAAGACCGTTGCTGAATCTGTTGGTGATTGGTACTTTGACAGAACAACAGTGAAAGCCATTGACTGTCCTTACCCCTGTGACAAAACATGTCACAATCTCATCTTCAAGTGA

Protein Analysis

416

Amino Acids

45.94

Weight (kDa)

6.9

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 49 - 411 4.5e-172 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 139
Acc36I ACCTGC 1 cut(s) 880
AccB1I GGYRCC 2 cut(s) 157, 455
AccB7I CCANNNNNTGG 1 cut(s) 832
AccII CGCG 1 cut(s) 1037
AccIII TCCGGA 2 cut(s) 321, 726
AciI CCGC 3 cut(s) 23, 26, 930
AclWI GGATC 4 cut(s) 256, 797, 810, 976
AcuI CTGAAG 1 cut(s) 985
AfaI GTAC 4 cut(s) 756, 790, 894, 1172
AfiI CCNNNNNNNGG 4 cut(s) 245, 280, 769, 832
AflII CTTAAG 1 cut(s) 670
AflIII ACRYGT 2 cut(s) 302, 1226
AgeI ACCGGT 1 cut(s) 261
AgsI TTSAA 3 cut(s) 421, 634, 1246
AjnI CCWGG 3 cut(s) 204, 220, 238
AluBI AGCT 9 cut(s) 148, 197, 257, 312, 489, 530, 596, 608, 908
AluI AGCT 9 cut(s) 148, 197, 257, 312, 489, 530, 596, 608, 908
Alw21I GWGCWC 1 cut(s) 491
Alw26I GTCTC 1 cut(s) 712
AlwI GGATC 4 cut(s) 256, 797, 810, 976
Ama87I CYCGRG 1 cut(s) 278
Aor13HI TCCGGA 2 cut(s) 321, 726
AoxI GGCC 2 cut(s) 369, 715
ApeKI GCWGC 4 cut(s) 145, 179, 182, 872
AsiGI ACCGGT 1 cut(s) 261
Asp700I GAANNNNTTC 2 cut(s) 642, 734
AspS9I GGNCC 1 cut(s) 715
AsuC2I CCSGG 1 cut(s) 648
AsuHPI GGTGA 3 cut(s) 142, 457, 1175
AvaI CYCGRG 1 cut(s) 278
BaeGI GKGCMC 1 cut(s) 653
BamHI GGATCC 1 cut(s) 802
BanI GGYRCC 2 cut(s) 157, 455
BanII GRGCYC 1 cut(s) 491
Bbv12I GWGCWC 1 cut(s) 491
BbvI GCAGC 4 cut(s) 157, 166, 169, 884
BccI CCATC 5 cut(s) 203, 285, 446, 620, 922
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 3 cut(s) 206, 222, 240
BclI TGATCA 1 cut(s) 955
BcnI CCSGG 1 cut(s) 648
BcoDI GTCTC 1 cut(s) 712
BfmI CTRYAG 1 cut(s) 502
BfrI CTTAAG 1 cut(s) 670
BfuAI ACCTGC 1 cut(s) 880
BglII AGATCT 1 cut(s) 102
BisI GCNGC 7 cut(s) 24, 27, 146, 180, 183, 873, 931
BlsI GCNGC 7 cut(s) 25, 28, 147, 181, 184, 874, 932
Bme1390I CCNGG 4 cut(s) 206, 222, 240, 648
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 715
BmiI GGNNCC 4 cut(s) 159, 457, 716, 804
BmrFI CCNGG 4 cut(s) 206, 222, 240, 648
BmsI GCATC 3 cut(s) 667, 685, 1009
BplI GAGNNNNNCTC 2 cut(s) 892, 924
Bpu10I CCTNAGC 1 cut(s) 926
BpuMI CCSGG 1 cut(s) 648
BsaAI YACGTR 1 cut(s) 305
BsaJI CCNNGG 3 cut(s) 238, 337, 739
BsaWI WCCGGW 3 cut(s) 261, 321, 726
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 280, 769, 832
Bse118I RCCGGY 2 cut(s) 261, 467
Bse1I ACTGG 3 cut(s) 382, 428, 985
BseAI TCCGGA 2 cut(s) 321, 726
BseBI CCWGG 3 cut(s) 206, 222, 240
BseDI CCNNGG 3 cut(s) 238, 337, 739
BseGI GGATG 8 cut(s) 214, 270, 296, 487, 593, 612, 700, 709
BseLI CCNNNNNNNGG 4 cut(s) 245, 280, 769, 832
BseMII CTCAG 1 cut(s) 992
BseNI ACTGG 3 cut(s) 382, 428, 985
BseSI GKGCMC 1 cut(s) 653
BseXI GCAGC 4 cut(s) 157, 166, 169, 884
BseYI CCCAGC 1 cut(s) 596
Bsh1236I CGCG 1 cut(s) 1037
BshFI GGCC 2 cut(s) 371, 717
BshNI GGYRCC 2 cut(s) 157, 455
BshTI ACCGGT 1 cut(s) 261
BsiHKAI GWGCWC 1 cut(s) 491
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 5 cut(s) 262, 322, 468, 647, 727
BslI CCNNNNNNNGG 4 cut(s) 245, 280, 769, 832
BsmAI GTCTC 1 cut(s) 712
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 2 cut(s) 371, 717
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 2 cut(s) 491, 653
Bsp13I TCCGGA 2 cut(s) 321, 726
Bsp1407I TGTACA 1 cut(s) 788
Bsp143I GATC 6 cut(s) 102, 248, 625, 802, 955, 981
Bsp68I TCGCGA 1 cut(s) 1037
BspACI CCGC 3 cut(s) 23, 26, 930
BspANI GGCC 2 cut(s) 371, 717
BspCNI CTCAG 1 cut(s) 991
BspEI TCCGGA 2 cut(s) 321, 726
BspFNI CGCG 1 cut(s) 1037
BspLI GGNNCC 4 cut(s) 159, 457, 716, 804
BspMI ACCTGC 1 cut(s) 880
BspPI GGATC 4 cut(s) 256, 797, 810, 976
BspT107I GGYRCC 2 cut(s) 157, 455
BspTI CTTAAG 1 cut(s) 670
BsrFI RCCGGY 2 cut(s) 261, 467
BsrGI TGTACA 1 cut(s) 788
BsrI ACTGG 3 cut(s) 382, 428, 985
BssAI RCCGGY 2 cut(s) 261, 467
BssECI CCNNGG 3 cut(s) 238, 337, 739
BssMI GATC 6 cut(s) 102, 248, 625, 802, 955, 981
BssT1I CCWWGG 1 cut(s) 739
Bst2UI CCWGG 3 cut(s) 206, 222, 240
Bst4CI ACNGT 7 cut(s) 661, 754, 777, 1055, 1147, 1189, 1205
Bst6I CTCTTC 1 cut(s) 1137
BstAFI CTTAAG 1 cut(s) 670
BstAUI TGTACA 1 cut(s) 788
BstBAI YACGTR 1 cut(s) 305
BstC8I GCNNGC 2 cut(s) 557, 946
BstDEI CTNAG 2 cut(s) 926, 978
BstDSI CCRYGG 1 cut(s) 337
BstENI CCTNNNNNAGG 1 cut(s) 767
BstF5I GGATG 8 cut(s) 214, 270, 296, 487, 593, 612, 700, 709
BstFNI CGCG 1 cut(s) 1037
BstKTI GATC 6 cut(s) 105, 251, 628, 805, 958, 984
BstMAI GTCTC 1 cut(s) 712
BstMBI GATC 6 cut(s) 102, 248, 625, 802, 955, 981
BstMWI GCNNNNNNNGC 2 cut(s) 572, 881
BstNI CCWGG 3 cut(s) 206, 222, 240
BstNSI RCATGY 3 cut(s) 559, 948, 1230
BstSCI CCNGG 4 cut(s) 204, 220, 238, 646
BstSFI CTRYAG 1 cut(s) 502
BstSLI GKGCMC 1 cut(s) 653
BstUI CGCG 1 cut(s) 1037
BstV1I GCAGC 4 cut(s) 157, 166, 169, 884
BstX2I RGATCY 3 cut(s) 102, 802, 981
BstYI RGATCY 3 cut(s) 102, 802, 981
BsuRI GGCC 2 cut(s) 371, 717
BtgI CCRYGG 1 cut(s) 337
BtgZI GCGATG 1 cut(s) 465
BtsCI GGATG 8 cut(s) 214, 270, 296, 487, 593, 612, 700, 709
BtsI GCAGTG 4 cut(s) 619, 705, 780, 1081
BtsIMutI CAGTG 7 cut(s) 375, 619, 666, 705, 780, 1081, 1194
BtuMI TCGCGA 1 cut(s) 1037
BveI ACCTGC 1 cut(s) 880
Cac8I GCNNGC 2 cut(s) 557, 946
Cfr10I RCCGGY 2 cut(s) 261, 467
Cfr13I GGNCC 1 cut(s) 715
CseI GACGC 1 cut(s) 890
CsiI ACCWGGT 1 cut(s) 220
Csp6I GTAC 4 cut(s) 755, 789, 893, 1171
CspAI ACCGGT 1 cut(s) 261
CviAII CATG 5 cut(s) 556, 945, 1071, 1136, 1227
CviQI GTAC 4 cut(s) 755, 789, 893, 1171
DdeI CTNAG 2 cut(s) 926, 978
DpnI GATC 6 cut(s) 104, 250, 627, 804, 957, 983
DpnII GATC 6 cut(s) 102, 248, 625, 802, 955, 981
DrdI GACNNNNNNGTC 1 cut(s) 139
DseDI GACNNNNNNGTC 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 1137
EarI CTCTTC 1 cut(s) 1137
Ecl136II GAGCTC 1 cut(s) 489
Eco130I CCWWGG 1 cut(s) 739
Eco24I GRGCYC 1 cut(s) 491
Eco53kI GAGCTC 1 cut(s) 489
Eco57I CTGAAG 1 cut(s) 985
Eco88I CYCGRG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 489
EcoNI CCTNNNNNAGG 1 cut(s) 767
EcoRII CCWGG 3 cut(s) 204, 220, 238
EcoT14I CCWWGG 1 cut(s) 739
EcoT38I GRGCYC 1 cut(s) 491
ErhI CCWWGG 1 cut(s) 739
FaeI CATG 5 cut(s) 559, 948, 1074, 1139, 1230
FalI AAGNNNNNCTT 2 cut(s) 919, 951
FatI CATG 5 cut(s) 555, 944, 1070, 1135, 1226
FauNDI CATATG 3 cut(s) 354, 877, 1011
FbaI TGATCA 1 cut(s) 955
Fnu4HI GCNGC 7 cut(s) 24, 27, 146, 180, 183, 873, 931
FokI GGATG 8 cut(s) 221, 257, 303, 494, 599, 600, 707, 716
FriOI GRGCYC 1 cut(s) 491
Fsp4HI GCNGC 7 cut(s) 24, 27, 146, 180, 183, 873, 931
GluI GCNGC 7 cut(s) 24, 27, 146, 180, 183, 873, 931
GsaI CCCAGC 1 cut(s) 600
HaeIII GGCC 2 cut(s) 371, 717
HapII CCGG 5 cut(s) 262, 322, 468, 647, 727
HgaI GACGC 1 cut(s) 890
Hin1II CATG 5 cut(s) 559, 948, 1074, 1139, 1230
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HinfI GANTC 8 cut(s) 172, 406, 432, 516, 850, 1006, 1121, 1154
HpaII CCGG 5 cut(s) 262, 322, 468, 647, 727
HphI GGTGA 3 cut(s) 142, 457, 1175
Hpy166II GTNNAC 2 cut(s) 780, 922
Hpy188I TCNGA 3 cut(s) 955, 981, 1093
Hpy188III TCNNGA 9 cut(s) 246, 252, 322, 410, 479, 585, 727, 1003, 1036
Hpy8I GTNNAC 2 cut(s) 780, 922
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 2 cut(s) 382, 934
HpyCH4III ACNGT 7 cut(s) 661, 754, 777, 1055, 1147, 1189, 1205
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 6 cut(s) 297, 559, 698, 872, 948, 1115
HpyF10VI GCNNNNNNNGC 2 cut(s) 572, 881
HpyF3I CTNAG 2 cut(s) 926, 978
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 5 cut(s) 559, 948, 1074, 1139, 1230
Kpn2I TCCGGA 2 cut(s) 321, 726
Ksp22I TGATCA 1 cut(s) 955
Kzo9I GATC 6 cut(s) 102, 248, 625, 802, 955, 981
LmnI GCTCC 5 cut(s) 194, 254, 309, 729, 913
Lsp1109I GCAGC 4 cut(s) 157, 166, 169, 884
LweI GCATC 3 cut(s) 667, 685, 1009
MabI ACCWGGT 1 cut(s) 220
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 6 cut(s) 108, 130, 443, 748, 1217, 1229
MalI GATC 6 cut(s) 104, 250, 627, 804, 957, 983
MboI GATC 6 cut(s) 102, 248, 625, 802, 955, 981
MboII GAAGA 8 cut(s) 16, 310, 409, 548, 854, 982, 1124, 1234
MfeI CAATTG 1 cut(s) 122
MflI RGATCY 3 cut(s) 102, 802, 981
MhlI GDGCHC 2 cut(s) 491, 653
MluCI AATT 3 cut(s) 122, 437, 970
MlyI GAGTC 3 cut(s) 441, 844, 1000
MmeI TCCRAC 2 cut(s) 298, 830
MnlI CCTC 7 cut(s) 171, 196, 274, 281, 469, 500, 773
MroI TCCGGA 2 cut(s) 321, 726
MroXI GAANNNNTTC 2 cut(s) 642, 734
MseI TTAA 2 cut(s) 671, 846
MslI CAYNNNNRTG 1 cut(s) 1053
MspA1I CMGCKG 1 cut(s) 596
MspCI CTTAAG 1 cut(s) 670
MspI CCGG 5 cut(s) 262, 322, 468, 647, 727
MspR9I CCNGG 4 cut(s) 206, 222, 240, 648
MunI CAATTG 1 cut(s) 122
Mva1269I GAATGC 1 cut(s) 523
MvaI CCWGG 3 cut(s) 206, 222, 240
MvnI CGCG 1 cut(s) 1037
MwoI GCNNNNNNNGC 2 cut(s) 572, 881
NciI CCSGG 1 cut(s) 648
NdeI CATATG 3 cut(s) 354, 877, 1011
NdeII GATC 6 cut(s) 102, 248, 625, 802, 955, 981
NlaIII CATG 5 cut(s) 559, 948, 1074, 1139, 1230
NlaIV GGNNCC 4 cut(s) 159, 457, 716, 804
NmuCI GTSAC 3 cut(s) 130, 1217, 1229
NruI TCGCGA 1 cut(s) 1037
NspI RCATGY 3 cut(s) 559, 948, 1230
PaeI GCATGC 2 cut(s) 559, 948
PaeR7I CTCGAG 1 cut(s) 278
PciI ACATGT 1 cut(s) 1226
PctI GAATGC 1 cut(s) 523
PdmI GAANNNNTTC 2 cut(s) 642, 734
PfeI GAWTC 5 cut(s) 172, 406, 516, 1121, 1154
PflMI CCANNNNNTGG 1 cut(s) 832
PinAI ACCGGT 1 cut(s) 261
PkrI GCNGC 7 cut(s) 25, 28, 147, 181, 184, 874, 932
PleI GAGTC 3 cut(s) 440, 844, 1000
PpsI GAGTC 3 cut(s) 440, 844, 1000
Ppu21I YACGTR 1 cut(s) 305
PscI ACATGT 1 cut(s) 1226
Psp124BI GAGCTC 1 cut(s) 491
Psp6I CCWGG 3 cut(s) 204, 220, 238
PspFI CCCAGC 1 cut(s) 596
PspGI CCWGG 3 cut(s) 204, 220, 238
PspN4I GGNNCC 4 cut(s) 159, 457, 716, 804
PspPI GGNCC 1 cut(s) 715
PspXI VCTCGAGB 1 cut(s) 278
PsrI GAACNNNNNNTAC 2 cut(s) 561, 593
PsuI RGATCY 3 cut(s) 102, 802, 981
PvuII CAGCTG 1 cut(s) 596
RruI TCGCGA 1 cut(s) 1037
RsaI GTAC 4 cut(s) 756, 790, 894, 1172
RsaNI GTAC 4 cut(s) 755, 789, 893, 1171
RseI CAYNNNNRTG 1 cut(s) 1053
SacI GAGCTC 1 cut(s) 491
SaqAI TTAA 2 cut(s) 671, 846
SatI GCNGC 7 cut(s) 24, 27, 146, 180, 183, 873, 931
Sau3AI GATC 6 cut(s) 102, 248, 625, 802, 955, 981
Sau96I GGNCC 1 cut(s) 715
SchI GAGTC 3 cut(s) 441, 844, 1000
ScrFI CCNGG 4 cut(s) 206, 222, 240, 648
SduI GDGCHC 2 cut(s) 491, 653
SexAI ACCWGGT 1 cut(s) 220
SfaNI GCATC 3 cut(s) 667, 685, 1009
SfcI CTRYAG 1 cut(s) 502
Sfr274I CTCGAG 1 cut(s) 278
SgrAI CRCCGGYG 1 cut(s) 467
SlaI CTCGAG 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 1053
SmlI CTYRAG 2 cut(s) 278, 670
SmoI CTYRAG 2 cut(s) 278, 670
SphI GCATGC 2 cut(s) 559, 948
Sse9I AATT 3 cut(s) 122, 437, 970
SsiI CCGC 3 cut(s) 23, 26, 930
SspI AATATT 1 cut(s) 384
SstI GAGCTC 1 cut(s) 491
StyD4I CCNGG 4 cut(s) 204, 220, 238, 646
StyI CCWWGG 1 cut(s) 739
TaaI ACNGT 7 cut(s) 661, 754, 777, 1055, 1147, 1189, 1205
TaiI ACGT 1 cut(s) 307
TaqI TCGA 3 cut(s) 114, 279, 1043
TasI AATT 3 cut(s) 122, 437, 970
TatI WGTACW 2 cut(s) 754, 788
TauI GCSGC 3 cut(s) 26, 29, 933
TfiI GAWTC 5 cut(s) 172, 406, 516, 1121, 1154
Tru1I TTAA 2 cut(s) 671, 846
Tru9I TTAA 2 cut(s) 671, 846
TscAI CASTG 7 cut(s) 382, 626, 666, 705, 787, 1088, 1194
TseFI GTSAC 3 cut(s) 130, 1217, 1229
TseI GCWGC 4 cut(s) 145, 179, 182, 872
Tsp45I GTSAC 3 cut(s) 130, 1217, 1229
TspDTI ATGAA 5 cut(s) 17, 333, 963, 1051, 1124
TspGWI ACGGA 1 cut(s) 129
TspRI CASTG 7 cut(s) 382, 626, 666, 705, 787, 1088, 1194
Van91I CCANNNNNTGG 1 cut(s) 832
Vha464I CTTAAG 1 cut(s) 670
XagI CCTNNNNNAGG 1 cut(s) 767
XceI RCATGY 3 cut(s) 559, 948, 1230
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 278
XmnI GAANNNNTTC 2 cut(s) 642, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.