FvH4_1g26120

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002705
Physical Location & Seq
Forward (+)
17990855 .. 17996496
5642 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g26120.t1

Sequence Viewer

Length: 1434 bp
ATGAACAAGTTTCAGCTGAAGCTGAAGCTGGATGGGTGTACAGTGTTGGTGGTAGTGGCATTGGCAGCTGTCTTTGTCAATCTGAATGTTTATGTGGGTGCTTTGAGTTTGAACGAACCAGTGAGTATTATTGAGAAAGATGCAGTCTTGTTTGAAGACGAACATGTCTTAGCCTCAGCCTCACCGACACAGCCTCAGCCCCTCTTGGTTGCTCTCACTCTTATTGACAACGCTGCTTCTAAAGGAGCGGTTTGTTTGGATGGAACGCCGCCGGGTTACCATCTGGATCGTGGGTCTGGATCGGGTGCAAACAGCTGGCTCATTCAGTTGGAGGGTGGGGGGTGGTGTAATACTATCAGAAACTGTGTTTACCGCAAGACCACTCGCCGCGGTTCATCCAAGTTCATGGAAAAGCAATTGCCCTTCACAGGAATTTTGAGCAATAAACCTGAAGAAAACCCCGATTTCTTCAACTGGAACAGGGTCAAGCTACGATACTGTGATGGGGCATCTTTTACTGGAGACAGCCAGAATGAGGCTGCTCAACTTAATTTTCGAGGGCAAAAGATTTGGTCAGTTGCTATGGAAGAGTTAATGTCCAAGGGAATGCAGAATGCTAACCAGGCTCTTCTTTCTGGATGTTCCGCTGGGGGTCTGGCATCGATACTACATTGTGATGAGTTCCGGGACTTGTTTCCAACCACTACCACAGTGAAATGTCTGAGTGATGCCGGGATGTTCCTTGATGCAATTGATGTATCTGGTGGGCACACTCTAAGGAATTTGTATGCAGGAGTAGTTAGCTTGCAGGAAGTGCAAAAGAATCTTCCGAAGACCTGTATCAACCACCTAGATCCAACTTCTTGCTTCTTTCCTCAGAATTTGGTTGCAAATGTTAAGACGCCAATGTTTCTTCTGAATGCAGCCTATGATGCTTGGCAGCTCCAAGCTAGTTTAGCCCCTCCATCTGCTGATCCTCATGGTTTATGGAATGAATGCAAATCAAACCATGCAGAATGTAATTCATCCCAGATCCAGTTTCTCCAAGATTTCAGGAATCAAATGCTCAATGCCGTGAAAGATTTTTCAATGTCCAGTGAAAATGGTTTATTCATAAACTCTTGCTTTGCTCACTGCCAGTCTGAGAGACAAGACACATGGTTTGCTGATGATTCTCCTCTTCTTGACAACCAGGCAATTGCGCTGTCTGTTGGAGACTGGTACTTCAATCGAAGGATGGTTAAAGCTATTGACTGTGCATTCCCCTGTGATAGCACCTGTCACAATCTTAACTTCAAAGAGGATATATCTTCTACGACATTGACATATTCTCAGTCCGCAAAGTTGTCTATTACCATGCTAAATCTGCCAAGTGCCTTGTTGATGCCTATAACATGTTCCAGCTTCATAATGTGGTTTCGATTTGTCCACTGA

Protein Analysis

478

Amino Acids

52.62

Weight (kDa)

5.59

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 65 - 428 6.2e-173 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AccBSI CCGCTC 1 cut(s) 248
AccII CGCG 1 cut(s) 390
AciI CCGC 7 cut(s) 248, 269, 373, 388, 390, 645, 1338
AclWI GGATC 5 cut(s) 294, 307, 848, 968, 1027
AcsI RAATTY 3 cut(s) 432, 781, 880
AcuI CTGAAG 3 cut(s) 38, 44, 471
AcyI GRCGYC 1 cut(s) 902
AfaI GTAC 2 cut(s) 40, 1223
AfiI CCNNNNNNNGG 2 cut(s) 428, 535
AflIII ACRYGT 2 cut(s) 163, 1394
AgsI TTSAA 6 cut(s) 112, 155, 472, 1089, 1228, 1297
AjnI CCWGG 2 cut(s) 621, 1191
Alw26I GTCTC 3 cut(s) 516, 1141, 1209
AlwI GGATC 5 cut(s) 294, 307, 848, 968, 1027
AlwNI CAGNNNCTG 1 cut(s) 363
ApeKI GCWGC 5 cut(s) 65, 233, 539, 923, 940
ApoI RAATTY 3 cut(s) 432, 781, 880
ArsI GACNNNNNNTTYG 2 cut(s) 1145, 1177
AspLEI GCGC 1 cut(s) 1204
AsuC2I CCSGG 3 cut(s) 273, 686, 733
AsuHPI GGTGA 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 771
BbsI GAAGAC 2 cut(s) 162, 839
BbvCI CCTCAGC 2 cut(s) 175, 195
BbvI GCAGC 5 cut(s) 77, 220, 526, 935, 952
BccI CCATC 6 cut(s) 26, 254, 288, 497, 973, 1231
BceAI ACGGC 1 cut(s) 1058
BciT130I CCWGG 2 cut(s) 623, 1193
BcnI CCSGG 3 cut(s) 273, 686, 733
BcoDI GTCTC 3 cut(s) 516, 1141, 1209
BfaI CTAG 2 cut(s) 851, 951
BisI GCNGC 7 cut(s) 66, 234, 269, 388, 540, 924, 941
BlsI GCNGC 7 cut(s) 67, 235, 270, 389, 541, 925, 942
Bme1390I CCNGG 5 cut(s) 273, 623, 686, 733, 1193
BmrFI CCNGG 5 cut(s) 273, 623, 686, 733, 1193
BmsI GCATC 7 cut(s) 130, 518, 668, 718, 736, 922, 1374
BpiI GAAGAC 2 cut(s) 162, 839
BpmI CTGGAG 1 cut(s) 540
Bpu10I CCTNAGC 2 cut(s) 175, 195
BpuMI CCSGG 3 cut(s) 273, 686, 733
Bsa29I ATCGAT 1 cut(s) 662
BsaHI GRCGYC 1 cut(s) 902
BsaJI CCNNGG 2 cut(s) 388, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 428, 535
Bse1I ACTGG 7 cut(s) 119, 479, 523, 1036, 1095, 1138, 1223
BseBI CCWGG 2 cut(s) 623, 1193
BseCI ATCGAT 1 cut(s) 662
BseDI CCNNGG 2 cut(s) 388, 600
BseGI GGATG 7 cut(s) 37, 265, 395, 644, 741, 1025, 1242
BseLI CCNNNNNNNGG 2 cut(s) 428, 535
BseMII CTCAG 6 cut(s) 189, 209, 713, 890, 1134, 1346
BseNI ACTGG 7 cut(s) 119, 479, 523, 1036, 1095, 1138, 1223
BseRI GAGGAG 1 cut(s) 1167
BseSI GKGCMC 1 cut(s) 771
BseXI GCAGC 5 cut(s) 77, 220, 526, 935, 952
BseYI CCCAGC 1 cut(s) 647
Bsh1236I CGCG 1 cut(s) 390
BshVI ATCGAT 1 cut(s) 662
BsiSI CCGG 3 cut(s) 272, 685, 732
BslFI GGGAC 1 cut(s) 701
BslI CCNNNNNNNGG 2 cut(s) 428, 535
BsmAI GTCTC 3 cut(s) 516, 1141, 1209
BsmFI GGGAC 1 cut(s) 701
BsmI GAATGC 5 cut(s) 612, 619, 925, 1001, 1259
Bsp1286I GDGCHC 1 cut(s) 771
Bsp1407I TGTACA 1 cut(s) 38
Bsp143I GATC 5 cut(s) 286, 299, 853, 973, 1032
BspACI CCGC 7 cut(s) 248, 269, 373, 388, 390, 645, 1338
BspCNI CTCAG 6 cut(s) 188, 208, 714, 889, 1135, 1345
BspDI ATCGAT 1 cut(s) 662
BspFNI CGCG 1 cut(s) 390
BspPI GGATC 5 cut(s) 294, 307, 848, 968, 1027
BspQI GCTCTTC 1 cut(s) 633
BsrBI CCGCTC 1 cut(s) 248
BsrGI TGTACA 1 cut(s) 38
BsrI ACTGG 7 cut(s) 119, 479, 523, 1036, 1095, 1138, 1223
BssECI CCNNGG 2 cut(s) 388, 600
BssMI GATC 5 cut(s) 286, 299, 853, 973, 1032
BssNI GRCGYC 1 cut(s) 902
BssT1I CCWWGG 1 cut(s) 600
Bst2UI CCWGG 2 cut(s) 623, 1193
Bst4CI ACNGT 5 cut(s) 43, 365, 500, 712, 1256
Bst6I CTCTTC 3 cut(s) 582, 633, 1185
BstACI GRCGYC 1 cut(s) 902
BstAPI GCANNNNNTGC 1 cut(s) 814
BstAUI TGTACA 1 cut(s) 38
BstC8I GCNNGC 2 cut(s) 317, 806
BstDEI CTNAG 8 cut(s) 169, 175, 195, 722, 776, 876, 1143, 1332
BstDSI CCRYGG 1 cut(s) 388
BstEII GGTNACC 1 cut(s) 275
BstF5I GGATG 7 cut(s) 37, 265, 395, 644, 741, 1025, 1242
BstFNI CGCG 1 cut(s) 390
BstHHI GCGC 1 cut(s) 1204
BstKTI GATC 5 cut(s) 289, 302, 856, 976, 1035
BstMAI GTCTC 3 cut(s) 516, 1141, 1209
BstMBI GATC 5 cut(s) 286, 299, 853, 973, 1032
BstMWI GCNNNNNNNGC 6 cut(s) 65, 623, 814, 932, 956, 1366
BstNI CCWGG 2 cut(s) 623, 1193
BstNSI RCATGY 2 cut(s) 167, 1398
BstPI GGTNACC 1 cut(s) 275
BstSCI CCNGG 5 cut(s) 271, 621, 684, 731, 1191
BstSLI GKGCMC 1 cut(s) 771
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 5 cut(s) 77, 220, 526, 935, 952
BstV2I GAAGAC 2 cut(s) 162, 839
BstX2I RGATCY 2 cut(s) 853, 1032
BstXI CCANNNNNNTGG 1 cut(s) 406
BstYI RGATCY 2 cut(s) 853, 1032
Bsu15I ATCGAT 1 cut(s) 662
BsuTUI ATCGAT 1 cut(s) 662
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 7 cut(s) 37, 265, 395, 644, 741, 1025, 1242
BtsI GCAGTG 1 cut(s) 1132
BtsIMutI CAGTG 6 cut(s) 48, 126, 717, 1102, 1132, 1429
Cac8I GCNNGC 2 cut(s) 317, 806
CaiI CAGNNNCTG 1 cut(s) 363
CfoI GCGC 1 cut(s) 1204
Cfr42I CCGCGG 1 cut(s) 391
ClaI ATCGAT 1 cut(s) 662
CseI GACGC 1 cut(s) 910
Csp6I GTAC 2 cut(s) 39, 1222
CviAII CATG 7 cut(s) 164, 406, 980, 1010, 1158, 1357, 1395
CviQI GTAC 2 cut(s) 39, 1222
DdeI CTNAG 8 cut(s) 169, 175, 195, 722, 776, 876, 1143, 1332
DpnI GATC 5 cut(s) 288, 301, 855, 975, 1034
DpnII GATC 5 cut(s) 286, 299, 853, 973, 1032
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
Eam1104I CTCTTC 3 cut(s) 582, 633, 1185
EarI CTCTTC 3 cut(s) 582, 633, 1185
Eco130I CCWWGG 1 cut(s) 600
Eco57I CTGAAG 3 cut(s) 38, 44, 471
Eco91I GGTNACC 1 cut(s) 275
EcoO65I GGTNACC 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 621, 1191
EcoT14I CCWWGG 1 cut(s) 600
ErhI CCWWGG 1 cut(s) 600
FaeI CATG 7 cut(s) 167, 409, 983, 1013, 1161, 1360, 1398
FaqI GGGAC 1 cut(s) 701
FatI CATG 7 cut(s) 163, 405, 979, 1009, 1157, 1356, 1394
Fnu4HI GCNGC 7 cut(s) 66, 234, 269, 388, 540, 924, 941
FokI GGATG 7 cut(s) 44, 272, 382, 651, 748, 1012, 1249
Fsp4HI GCNGC 7 cut(s) 66, 234, 269, 388, 540, 924, 941
FspBI CTAG 2 cut(s) 851, 951
GlaI GCGC 1 cut(s) 1203
GluI GCNGC 7 cut(s) 66, 234, 269, 388, 540, 924, 941
GsaI CCCAGC 1 cut(s) 651
GsuI CTGGAG 1 cut(s) 540
HapII CCGG 3 cut(s) 272, 685, 732
HgaI GACGC 1 cut(s) 910
HhaI GCGC 1 cut(s) 1204
Hin1I GRCGYC 1 cut(s) 902
Hin1II CATG 7 cut(s) 167, 409, 983, 1013, 1161, 1360, 1398
Hin6I GCGC 1 cut(s) 1202
HinP1I GCGC 1 cut(s) 1202
HinfI GANTC 3 cut(s) 823, 1057, 1172
HpaII CCGG 3 cut(s) 272, 685, 732
HphI GGTGA 1 cut(s) 174
Hpy166II GTNNAC 3 cut(s) 39, 370, 1429
Hpy188I TCNGA 7 cut(s) 84, 359, 723, 831, 879, 918, 1144
Hpy188III TCNNGA 5 cut(s) 284, 297, 636, 1054, 1184
Hpy8I GTNNAC 3 cut(s) 39, 370, 1429
HpyAV CCTTC 2 cut(s) 433, 1227
HpyCH4III ACNGT 5 cut(s) 43, 365, 500, 712, 1256
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 623, 814, 932, 956, 1366
HpyF3I CTNAG 8 cut(s) 169, 175, 195, 722, 776, 876, 1143, 1332
Hsp92I GRCGYC 1 cut(s) 902
Hsp92II CATG 7 cut(s) 167, 409, 983, 1013, 1161, 1360, 1398
HspAI GCGC 1 cut(s) 1202
KspI CCGCGG 1 cut(s) 391
Kzo9I GATC 5 cut(s) 286, 299, 853, 973, 1032
LguI GCTCTTC 1 cut(s) 633
LmnI GCTCC 2 cut(s) 245, 948
Lsp1109I GCAGC 5 cut(s) 77, 220, 526, 935, 952
LweI GCATC 7 cut(s) 130, 518, 668, 718, 736, 922, 1374
MaeI CTAG 2 cut(s) 851, 951
MaeIII GTNAC 2 cut(s) 275, 1280
MalI GATC 5 cut(s) 288, 301, 855, 975, 1034
MbiI CCGCTC 1 cut(s) 248
MboI GATC 5 cut(s) 286, 299, 853, 973, 1032
MfeI CAATTG 3 cut(s) 416, 750, 1197
MflI RGATCY 2 cut(s) 853, 1032
MhlI GDGCHC 1 cut(s) 771
MluCI AATT 8 cut(s) 416, 432, 550, 750, 781, 880, 1021, 1197
MmeI TCCRAC 4 cut(s) 309, 722, 881, 1192
MseI TTAA 5 cut(s) 549, 593, 897, 1242, 1290
MslI CAYNNNNRTG 1 cut(s) 675
MspA1I CMGCKG 5 cut(s) 16, 68, 315, 390, 647
MspI CCGG 3 cut(s) 272, 685, 732
MspR9I CCNGG 5 cut(s) 273, 623, 686, 733, 1193
MunI CAATTG 3 cut(s) 416, 750, 1197
Mva1269I GAATGC 5 cut(s) 612, 619, 925, 1001, 1259
MvaI CCWGG 2 cut(s) 623, 1193
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 6 cut(s) 65, 623, 814, 932, 956, 1366
NciI CCSGG 3 cut(s) 273, 686, 733
NdeII GATC 5 cut(s) 286, 299, 853, 973, 1032
NlaIII CATG 7 cut(s) 167, 409, 983, 1013, 1161, 1360, 1398
NmuCI GTSAC 1 cut(s) 1280
NspI RCATGY 2 cut(s) 167, 1398
PciI ACATGT 2 cut(s) 163, 1394
PciSI GCTCTTC 1 cut(s) 633
PctI GAATGC 5 cut(s) 612, 619, 925, 1001, 1259
PfeI GAWTC 3 cut(s) 823, 1057, 1172
PfoI TCCNGGA 1 cut(s) 684
PkrI GCNGC 7 cut(s) 67, 235, 270, 389, 541, 925, 942
PscI ACATGT 2 cut(s) 163, 1394
Psp6I CCWGG 2 cut(s) 621, 1191
PspEI GGTNACC 1 cut(s) 275
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 2 cut(s) 621, 1191
PstNI CAGNNNCTG 1 cut(s) 363
PsuI RGATCY 2 cut(s) 853, 1032
PvuII CAGCTG 3 cut(s) 16, 68, 315
RsaI GTAC 2 cut(s) 40, 1223
RsaNI GTAC 2 cut(s) 39, 1222
RseI CAYNNNNRTG 1 cut(s) 675
SacII CCGCGG 1 cut(s) 391
SapI GCTCTTC 1 cut(s) 633
SaqAI TTAA 5 cut(s) 549, 593, 897, 1242, 1290
SatI GCNGC 7 cut(s) 66, 234, 269, 388, 540, 924, 941
Sau3AI GATC 5 cut(s) 286, 299, 853, 973, 1032
ScrFI CCNGG 5 cut(s) 273, 623, 686, 733, 1193
SduI GDGCHC 1 cut(s) 771
SfaNI GCATC 7 cut(s) 130, 518, 668, 718, 736, 922, 1374
Sfr303I CCGCGG 1 cut(s) 391
SgrBI CCGCGG 1 cut(s) 391
SmiMI CAYNNNNRTG 1 cut(s) 675
Sse9I AATT 8 cut(s) 416, 432, 550, 750, 781, 880, 1021, 1197
SsiI CCGC 7 cut(s) 248, 269, 373, 388, 390, 645, 1338
SspMI CTAG 2 cut(s) 851, 951
StyD4I CCNGG 5 cut(s) 271, 621, 684, 731, 1191
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 5 cut(s) 43, 365, 500, 712, 1256
TaqI TCGA 4 cut(s) 556, 662, 1231, 1420
TasI AATT 8 cut(s) 416, 432, 550, 750, 781, 880, 1021, 1197
TatI WGTACW 1 cut(s) 38
TauI GCSGC 2 cut(s) 271, 390
TfiI GAWTC 3 cut(s) 823, 1057, 1172
Tru1I TTAA 5 cut(s) 549, 593, 897, 1242, 1290
Tru9I TTAA 5 cut(s) 549, 593, 897, 1242, 1290
TscAI CASTG 5 cut(s) 48, 126, 717, 1102, 1139
TseFI GTSAC 1 cut(s) 1280
TseI GCWGC 5 cut(s) 65, 233, 539, 923, 940
Tsp45I GTSAC 1 cut(s) 1280
TspDTI ATGAA 7 cut(s) 17, 384, 394, 1008, 1014, 1102, 1396
TspRI CASTG 5 cut(s) 48, 126, 717, 1102, 1139
XapI RAATTY 3 cut(s) 432, 781, 880
XceI RCATGY 2 cut(s) 167, 1398
XcmI CCANNNNNNNNNTGG 1 cut(s) 287
XspI CTAG 2 cut(s) 851, 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.