Rh3CG303000

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0002705
Physical Location & Seq
Forward (+)
31525875 .. 31530918
5044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG303000.1

Sequence Viewer

Length: 1425 bp
ATGAAGAAGAAGCAGCAGCAGCTGAATGTGGGTACAGTGTTGGTGGTGGCATTGGCAGCTGCCTTTGTCAATCCGAAACTCAATGTGGGTGCTTTGAGTTTGAACGAATCAGTGAACGTTGGGAGAAAAGATGCAGTCTTGTTCGACAACGATCATGTCTCAGCCTCAGCCTCACCGACACAGCCTCAGCCTCTCATGGTGGCCCTCACTCTTATCGACAGCGCTGCTTCTAAAGGAGCTGTCTGTTTGGATGGAACACCGCCCGGTTACCATCTGGATCGTGGGTCTGGATCCGGTGCAAACAGCTGGCTCATTCAGTTGGAGGGAGGTGGGTGGTGTAATACTATCAGAAATTGTGTTTATCGCAAGACCACTCGCCGCGGTTCATCCAACCACATGGAAAAGCAATTACCATTCACAGGAATTTTGAGCAATAAACCTGAAGAAAACCCCGACTTCTTCAACTGGAACAGGGTCAAGCTACGATACTGTGATGGGGCATCTTTTGCCGGAGACAGCCAGAATGAGGCTGCACAACTTAATTTTCGAGGGCAAAAGATTTGGTTGGTTGCTATGGAAGAGTTGATGTCTAAGGGAATGCAGAATGCTAACCAGGCTCTTCTTTCTGGATGTTCCGCTGGGGGTCTGGCATCCATACTACACTGTGATGAGTTCCGGGACTTGTTTCCAACCAGTACCACAGTGAAATGTCTGAGTGATGCTGGGATGTTCCTTGATGCAATTGATGTATCTGGTGGGCACACTCTAAGGAATTTGTATGCAGGAGTAGTTAGCTTGCAGGAAGTGCAAAAGAATCTTCCAAAGACCTGTATCAACCACCTAGATCCAACTTCGTGCTTTTTTCCTCAGAATTTGGTCGCAAATGTTAAGACGCCAATGTTTCTTCTAAATGCAGCCTATGATGCTTGGCAGCTCCAAGCTAGTTTAGCCCCTCCATCTGCTGATCCTAATGGTTTATGGAATGAATGCAAATCAAACCATGCAAATTGTAATTTATCCCAGATCCAGTTTCTCCAAGATTTCAGGAATCAAATGCTCAGTGCTGTGAAAGATTTCTCAATGTCCCGTGAAAATGGTTTATTCATAAACTCTTGCTTTGCTCACTGCCAGTCTGAGAGACAAGATACATGGTTTGCTGATGATTCTCCTGTTCTTGAAAACCAGCCAATTGCACTGTCTGTTGGAGACTGGTACTTTGATCGAAGGACAGTTAAAGCTATTGACTGTGAATACCCCTGTGATAGCAGCTGTCACAATCTTAACTTCAAAGAGGATGTAACTTCTACGACATTGACATATTCTCAGTCTGCAAAGTCGTCTATTACCATGCTAAATCTGCTAGGTGCCTTGTTGATGCCTATAACGTGTTCGAGATGCATAACGTGGTTTTCATTTGTCCACTGA

Protein Analysis

474

Amino Acids

52.06

Weight (kDa)

6.07

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 62 - 425 1.3e-173 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1364
AccII CGCG 1 cut(s) 381
AciI CCGC 4 cut(s) 260, 379, 381, 636
AclI AACGTT 1 cut(s) 117
AclWI GGATC 6 cut(s) 285, 285, 298, 839, 959, 1018
AcsI RAATTY 3 cut(s) 423, 772, 871
AcuI CTGAAG 1 cut(s) 462
AcyI GRCGYC 1 cut(s) 893
AfaI GTAC 3 cut(s) 34, 697, 1214
AfeI AGCGCT 1 cut(s) 223
AfiI CCNNNNNNNGG 2 cut(s) 419, 526
AflIII ACRYGT 1 cut(s) 1385
AgsI TTSAA 4 cut(s) 103, 463, 1178, 1288
AjnI CCWGG 1 cut(s) 612
Alw26I GTCTC 4 cut(s) 163, 507, 1132, 1200
AlwI GGATC 6 cut(s) 285, 285, 298, 839, 959, 1018
AlwNI CAGNNNCTG 1 cut(s) 22
Aor51HI AGCGCT 1 cut(s) 223
AoxI GGCC 1 cut(s) 201
ApoI RAATTY 3 cut(s) 423, 772, 871
ArsI GACNNNNNNTTYG 2 cut(s) 1199, 1231
Asp700I GAANNNNTTC 1 cut(s) 1073
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 1 cut(s) 202
AsuC2I CCSGG 2 cut(s) 264, 677
AsuHPI GGTGA 1 cut(s) 165
BaeGI GKGCMC 1 cut(s) 762
BamHI GGATCC 1 cut(s) 290
BanI GGYRCC 1 cut(s) 1364
BbvCI CCTCAGC 2 cut(s) 166, 186
BccI CCATC 4 cut(s) 245, 279, 488, 964
BcgI CGANNNNNNTGC 2 cut(s) 206, 240
BciT130I CCWGG 1 cut(s) 614
BcnI CCSGG 2 cut(s) 264, 677
BcoDI GTCTC 4 cut(s) 163, 507, 1132, 1200
BfaI CTAG 3 cut(s) 842, 942, 1361
BfoI RGCGCY 1 cut(s) 225
Bme1390I CCNGG 3 cut(s) 264, 614, 677
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 2 cut(s) 292, 1366
BmrFI CCNGG 3 cut(s) 264, 614, 677
BmsI GCATC 8 cut(s) 121, 509, 659, 709, 727, 913, 1365, 1385
Bpu10I CCTNAGC 2 cut(s) 166, 186
BpuMI CCSGG 2 cut(s) 264, 677
BsaHI GRCGYC 1 cut(s) 893
BsaJI CCNNGG 1 cut(s) 379
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 526
Bse1I ACTGG 5 cut(s) 470, 693, 1027, 1129, 1214
BseBI CCWGG 1 cut(s) 614
BseDI CCNNGG 1 cut(s) 379
BseGI GGATG 6 cut(s) 256, 386, 635, 650, 732, 1300
BseLI CCNNNNNNNGG 2 cut(s) 419, 526
BseMII CTCAG 8 cut(s) 174, 180, 200, 704, 881, 1072, 1125, 1337
BseNI ACTGG 5 cut(s) 470, 693, 1027, 1129, 1214
BseSI GKGCMC 1 cut(s) 762
BseYI CCCAGC 2 cut(s) 638, 722
BsgI GTGCAG 1 cut(s) 516
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 1 cut(s) 203
BshNI GGYRCC 1 cut(s) 1364
BsiSI CCGG 4 cut(s) 264, 294, 510, 676
BslFI GGGAC 2 cut(s) 692, 1069
BslI CCNNNNNNNGG 2 cut(s) 419, 526
BsmAI GTCTC 4 cut(s) 163, 507, 1132, 1200
BsmFI GGGAC 2 cut(s) 692, 1069
BsmI GAATGC 3 cut(s) 603, 610, 992
BsnI GGCC 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 762
Bsp143I GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
BspACI CCGC 4 cut(s) 260, 379, 381, 636
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 8 cut(s) 173, 179, 199, 705, 880, 1071, 1126, 1336
BspFNI CGCG 1 cut(s) 381
BspLI GGNNCC 2 cut(s) 292, 1366
BspPI GGATC 6 cut(s) 285, 285, 298, 839, 959, 1018
BspQI GCTCTTC 1 cut(s) 624
BspT107I GGYRCC 1 cut(s) 1364
BsrI ACTGG 5 cut(s) 470, 693, 1027, 1129, 1214
BssECI CCNNGG 1 cut(s) 379
BssMI GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
BssNI GRCGYC 1 cut(s) 893
Bst2UI CCWGG 1 cut(s) 614
Bst4CI ACNGT 7 cut(s) 37, 491, 665, 703, 1197, 1231, 1247
Bst6I CTCTTC 2 cut(s) 573, 624
BstACI GRCGYC 1 cut(s) 893
BstAPI GCANNNNNTGC 2 cut(s) 506, 805
BstC8I GCNNGC 2 cut(s) 308, 797
BstDSI CCRYGG 1 cut(s) 379
BstEII GGTNACC 1 cut(s) 266
BstF5I GGATG 6 cut(s) 256, 386, 635, 650, 732, 1300
BstFNI CGCG 1 cut(s) 381
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 7 cut(s) 154, 280, 293, 847, 967, 1026, 1222
BstMAI GTCTC 4 cut(s) 163, 507, 1132, 1200
BstMBI GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
BstMWI GCNNNNNNNGC 8 cut(s) 19, 56, 506, 614, 805, 923, 947, 1357
BstNI CCWGG 1 cut(s) 614
BstPI GGTNACC 1 cut(s) 266
BstSCI CCNGG 3 cut(s) 262, 612, 675
BstSLI GKGCMC 1 cut(s) 762
BstUI CGCG 1 cut(s) 381
BstX2I RGATCY 3 cut(s) 290, 844, 1023
BstXI CCANNNNNNTGG 1 cut(s) 397
BstYI RGATCY 3 cut(s) 290, 844, 1023
BsuRI GGCC 1 cut(s) 203
BtgI CCRYGG 1 cut(s) 379
BtsCI GGATG 6 cut(s) 256, 386, 635, 650, 732, 1300
BtsI GCAGTG 1 cut(s) 1123
BtsIMutI CAGTG 8 cut(s) 42, 117, 661, 708, 1066, 1123, 1193, 1420
Cac8I GCNNGC 2 cut(s) 308, 797
CaiI CAGNNNCTG 1 cut(s) 22
CfoI GCGC 1 cut(s) 224
Cfr13I GGNCC 1 cut(s) 202
Cfr42I CCGCGG 1 cut(s) 382
CseI GACGC 1 cut(s) 901
Csp6I GTAC 3 cut(s) 33, 696, 1213
CviAII CATG 6 cut(s) 155, 196, 397, 1001, 1149, 1348
CviQI GTAC 3 cut(s) 33, 696, 1213
DpnI GATC 7 cut(s) 153, 279, 292, 846, 966, 1025, 1221
DpnII GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
Eam1104I CTCTTC 2 cut(s) 573, 624
EarI CTCTTC 2 cut(s) 573, 624
Eco47III AGCGCT 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 462
Eco91I GGTNACC 1 cut(s) 266
EcoO65I GGTNACC 1 cut(s) 266
EcoRII CCWGG 1 cut(s) 612
EcoT22I ATGCAT 1 cut(s) 1400
FaeI CATG 6 cut(s) 158, 199, 400, 1004, 1152, 1351
FaqI GGGAC 2 cut(s) 692, 1069
FatI CATG 6 cut(s) 154, 195, 396, 1000, 1148, 1347
FokI GGATG 6 cut(s) 263, 373, 637, 642, 739, 1307
FspBI CTAG 3 cut(s) 842, 942, 1361
GlaI GCGC 1 cut(s) 223
GsaI CCCAGC 2 cut(s) 642, 726
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 1 cut(s) 203
HapII CCGG 4 cut(s) 264, 294, 510, 676
HgaI GACGC 1 cut(s) 901
HhaI GCGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 893
Hin1II CATG 6 cut(s) 158, 199, 400, 1004, 1152, 1351
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HinfI GANTC 4 cut(s) 107, 814, 1048, 1163
HpaII CCGG 4 cut(s) 264, 294, 510, 676
HphI GGTGA 1 cut(s) 165
Hpy166II GTNNAC 2 cut(s) 115, 1420
Hpy188I TCNGA 5 cut(s) 75, 350, 714, 870, 1135
Hpy188III TCNNGA 6 cut(s) 275, 288, 627, 1045, 1175, 1392
Hpy8I GTNNAC 2 cut(s) 115, 1420
HpyAV CCTTC 1 cut(s) 1218
HpyCH4III ACNGT 7 cut(s) 37, 491, 665, 703, 1197, 1231, 1247
HpyCH4IV ACGT 3 cut(s) 117, 1385, 1403
HpyF10VI GCNNNNNNNGC 8 cut(s) 19, 56, 506, 614, 805, 923, 947, 1357
HpySE526I ACGT 3 cut(s) 117, 1385, 1403
Hsp92I GRCGYC 1 cut(s) 893
Hsp92II CATG 6 cut(s) 158, 199, 400, 1004, 1152, 1351
HspAI GCGC 1 cut(s) 222
KspI CCGCGG 1 cut(s) 382
Kzo9I GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
LguI GCTCTTC 1 cut(s) 624
LmnI GCTCC 2 cut(s) 236, 939
LweI GCATC 8 cut(s) 121, 509, 659, 709, 727, 913, 1365, 1385
MaeI CTAG 3 cut(s) 842, 942, 1361
MaeII ACGT 3 cut(s) 117, 1385, 1403
MaeIII GTNAC 3 cut(s) 266, 1271, 1297
MalI GATC 7 cut(s) 153, 279, 292, 846, 966, 1025, 1221
MboI GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
MboII GAAGA 8 cut(s) 16, 19, 451, 455, 590, 611, 809, 896
MfeI CAATTG 2 cut(s) 741, 1188
MflI RGATCY 3 cut(s) 290, 844, 1023
MhlI GDGCHC 1 cut(s) 762
MmeI TCCRAC 5 cut(s) 300, 414, 713, 872, 1183
Mph1103I ATGCAT 1 cut(s) 1400
MroXI GAANNNNTTC 1 cut(s) 1073
MseI TTAA 4 cut(s) 540, 888, 1233, 1281
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 6 cut(s) 22, 59, 306, 381, 638, 1269
MspI CCGG 4 cut(s) 264, 294, 510, 676
MspR9I CCNGG 3 cut(s) 264, 614, 677
MunI CAATTG 2 cut(s) 741, 1188
Mva1269I GAATGC 3 cut(s) 603, 610, 992
MvaI CCWGG 1 cut(s) 614
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 8 cut(s) 19, 56, 506, 614, 805, 923, 947, 1357
NciI CCSGG 2 cut(s) 264, 677
NdeII GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
NlaIII CATG 6 cut(s) 158, 199, 400, 1004, 1152, 1351
NlaIV GGNNCC 2 cut(s) 292, 1366
NmuCI GTSAC 1 cut(s) 1271
NsiI ATGCAT 1 cut(s) 1400
PciSI GCTCTTC 1 cut(s) 624
PctI GAATGC 3 cut(s) 603, 610, 992
PdmI GAANNNNTTC 1 cut(s) 1073
PfeI GAWTC 4 cut(s) 107, 814, 1048, 1163
PfoI TCCNGGA 1 cut(s) 675
Psp1406I AACGTT 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 612
PspEI GGTNACC 1 cut(s) 266
PspFI CCCAGC 2 cut(s) 638, 722
PspGI CCWGG 1 cut(s) 612
PspN4I GGNNCC 2 cut(s) 292, 1366
PspPI GGNCC 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 22
PsuI RGATCY 3 cut(s) 290, 844, 1023
PvuII CAGCTG 4 cut(s) 22, 59, 306, 1269
RsaI GTAC 3 cut(s) 34, 697, 1214
RsaNI GTAC 3 cut(s) 33, 696, 1213
RseI CAYNNNNRTG 1 cut(s) 666
SacII CCGCGG 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 624
SaqAI TTAA 4 cut(s) 540, 888, 1233, 1281
Sau3AI GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
Sau96I GGNCC 1 cut(s) 202
ScrFI CCNGG 3 cut(s) 264, 614, 677
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 8 cut(s) 121, 509, 659, 709, 727, 913, 1365, 1385
Sfr303I CCGCGG 1 cut(s) 382
SgrBI CCGCGG 1 cut(s) 382
SmiMI CAYNNNNRTG 1 cut(s) 666
SsiI CCGC 4 cut(s) 260, 379, 381, 636
SspMI CTAG 3 cut(s) 842, 942, 1361
StyD4I CCNGG 3 cut(s) 262, 612, 675
TaaI ACNGT 7 cut(s) 37, 491, 665, 703, 1197, 1231, 1247
TaiI ACGT 3 cut(s) 120, 1388, 1406
TaqI TCGA 5 cut(s) 144, 216, 547, 1222, 1391
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 4 cut(s) 107, 814, 1048, 1163
Tru1I TTAA 4 cut(s) 540, 888, 1233, 1281
Tru9I TTAA 4 cut(s) 540, 888, 1233, 1281
TscAI CASTG 7 cut(s) 42, 117, 668, 708, 1066, 1130, 1200
TseFI GTSAC 1 cut(s) 1271
Tsp45I GTSAC 1 cut(s) 1271
TspDTI ATGAA 5 cut(s) 17, 375, 999, 1093, 1401
TspRI CASTG 7 cut(s) 42, 117, 668, 708, 1066, 1130, 1200
XapI RAATTY 3 cut(s) 423, 772, 871
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 1073
XspI CTAG 3 cut(s) 842, 942, 1361
Zsp2I ATGCAT 1 cut(s) 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.