Rw6G003830

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002705
Physical Location & Seq
Forward (+)
6231681 .. 6236265
4585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003830.1

Sequence Viewer

Length: 993 bp
ATGTCACAACCCCCACTATGGTGGAAGCGTTTGCTGCTAGATTGGCTTGTCATCTGTTTGGATGGAACACTGCCGGGTTACCATCTGGATCAGGTTCAAACAGCTGGCTCATTCAGTTGGAGGTGGGTTGGGTTCAACCATTTCTCAGGAGGTGGGTGGTGTAATACAATCAGAAATTGTATTAATCACAAGACCACTCGCTGCGGTTCATCCAAGTACATGGAAAAGCAATTGCCATTCACAGGAATTTTGAGATATAAACCTGCAGAAAACCTTGATTTCTTCAACTGGAACAGGGTCAAGCTACGATACTGTGATGGGGCATCTTTTACTGGAGACAGGCAAAATATTTGGTTAGTTGCTATTGATGAGTTTATGTCCAAGGGAATGCAGAATGCTAACCAGGCTCTTCTTTCTGGATGTTCCGCTGGGGTTCTGGCATCCATACTACACTGTGATGAGTTCTGGGACTTGTTTCCAACCAAATTGATGTATTTGGTGGGCACACTCTTAAGGAATTTGAAAGCAGGAGTAGTTAGCTTGCAGGAAGTGCAAAACAATATTCCGAAGACTTGTATCAACCACCTAGATCCAACTTCTTGCATCGTTCCCCAGAATTTAGTTGCAAATGTTAAGACGCCAATGTTTCTTATAAATGCAGCCTATGATGCTTGGCAGCTCCAAGCTAGTTTAGCCCCTCCATCCGGTGATCCTCATGGTTTATGGAATGAATGCAAATCAAACCATGCAAATTGTAATTCATCACATATCCAGCATCTCCAAGATTTCAGGAAGCAAATGCTCAGCGCTGTGAAAGATTTCTCAGTGACCAGTGAAAATGGTTTATTCATAAGCTCTTGCTTTGCTCGCTGCCAGTCTGAGAGACAATCTACATGGTTTGCTGATGATGATTATCCTCTTCTTGAAAACCAGCTCAATTCTTTTTTATGCAGGCAATTGCACTTTTCTGTTGGAGACTGGTACTTTGATTGA

Protein Analysis

330

Amino Acids

37.6

Weight (kDa)

6.77

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 17 - 330 6e-95 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 653
Acc36I ACCTGC 1 cut(s) 271
AciI CCGC 2 cut(s) 204, 426
AclWI GGATC 3 cut(s) 96, 584, 704
AcsI RAATTY 3 cut(s) 246, 517, 616
AcyI GRCGYC 1 cut(s) 638
AfaI GTAC 2 cut(s) 218, 983
AfeI AGCGCT 1 cut(s) 808
AfiI CCNNNNNNNGG 3 cut(s) 18, 242, 704
AflII CTTAAG 1 cut(s) 511
AgsI TTSAA 5 cut(s) 98, 136, 286, 523, 926
AjnI CCWGG 1 cut(s) 402
AleI CACNNNNGTG 1 cut(s) 19
AluBI AGCT 7 cut(s) 104, 304, 540, 679, 686, 855, 934
AluI AGCT 7 cut(s) 104, 304, 540, 679, 686, 855, 934
Alw26I GTCTC 3 cut(s) 330, 877, 969
AlwI GGATC 3 cut(s) 96, 584, 704
Aor51HI AGCGCT 1 cut(s) 808
ApeKI GCWGC 5 cut(s) 34, 201, 659, 676, 870
ApoI RAATTY 3 cut(s) 246, 517, 616
ArsI GACNNNNNNTTYG 1 cut(s) 968
AseI ATTAAT 1 cut(s) 183
Asp700I GAANNNNTTC 1 cut(s) 818
AspLEI GCGC 1 cut(s) 809
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 719
BaeGI GKGCMC 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 575
BbvI GCAGC 5 cut(s) 21, 188, 671, 688, 857
BccI CCATC 4 cut(s) 56, 90, 311, 709
BciT130I CCWGG 1 cut(s) 404
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 3 cut(s) 330, 877, 969
BfaI CTAG 3 cut(s) 38, 587, 687
BfmI CTRYAG 1 cut(s) 264
BfoI RGCGCY 1 cut(s) 810
BfrI CTTAAG 1 cut(s) 511
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 5 cut(s) 35, 202, 660, 677, 871
BlpI GCTNAGC 1 cut(s) 803
BlsI GCNGC 5 cut(s) 36, 203, 661, 678, 872
Bme1390I CCNGG 2 cut(s) 75, 404
BmrFI CCNGG 2 cut(s) 75, 404
BmsI GCATC 5 cut(s) 332, 449, 612, 658, 784
BpiI GAAGAC 1 cut(s) 575
BpmI CTGGAG 1 cut(s) 354
Bpu1102I GCTNAGC 1 cut(s) 803
BpuMI CCSGG 1 cut(s) 75
BsaBI GATNNNNATC 1 cut(s) 912
BsaHI GRCGYC 1 cut(s) 638
BsaJI CCNNGG 1 cut(s) 381
BsaWI WCCGGW 1 cut(s) 704
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 242, 704
Bse1I ACTGG 5 cut(s) 293, 337, 831, 874, 983
Bse8I GATNNNNATC 1 cut(s) 912
BseBI CCWGG 1 cut(s) 404
BseDI CCNNGG 1 cut(s) 381
BseGI GGATG 5 cut(s) 67, 209, 425, 440, 701
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 3 cut(s) 18, 242, 704
BseMII CTCAG 4 cut(s) 159, 817, 837, 870
BseNI ACTGG 5 cut(s) 293, 337, 831, 874, 983
BseSI GKGCMC 1 cut(s) 506
BseXI GCAGC 5 cut(s) 21, 188, 671, 688, 857
BseYI CCCAGC 1 cut(s) 428
BsiSI CCGG 2 cut(s) 74, 705
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 3 cut(s) 18, 242, 704
BsmAI GTCTC 3 cut(s) 330, 877, 969
BsmFI GGGAC 1 cut(s) 482
BsmI GAATGC 3 cut(s) 393, 400, 737
Bsp1286I GDGCHC 1 cut(s) 506
Bsp143I GATC 3 cut(s) 88, 589, 709
Bsp1720I GCTNAGC 1 cut(s) 803
BspACI CCGC 2 cut(s) 204, 426
BspCNI CTCAG 4 cut(s) 158, 816, 836, 871
BspMAI CTGCAG 1 cut(s) 268
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 3 cut(s) 96, 584, 704
BspQI GCTCTTC 1 cut(s) 414
BspTI CTTAAG 1 cut(s) 511
BsrI ACTGG 5 cut(s) 293, 337, 831, 874, 983
BssECI CCNNGG 1 cut(s) 381
BssMI GATC 3 cut(s) 88, 589, 709
BssNI GRCGYC 1 cut(s) 638
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 404
Bst4CI ACNGT 2 cut(s) 314, 455
Bst6I CTCTTC 2 cut(s) 414, 924
BstACI GRCGYC 1 cut(s) 638
BstAFI CTTAAG 1 cut(s) 511
BstAPI GCANNNNNTGC 1 cut(s) 550
BstC8I GCNNGC 4 cut(s) 106, 542, 868, 953
BstDEI CTNAG 4 cut(s) 145, 803, 823, 879
BstEII GGTNACC 1 cut(s) 77
BstF5I GGATG 5 cut(s) 67, 209, 425, 440, 701
BstH2I RGCGCY 1 cut(s) 810
BstHHI GCGC 1 cut(s) 809
BstKTI GATC 3 cut(s) 91, 592, 712
BstMAI GTCTC 3 cut(s) 330, 877, 969
BstMBI GATC 3 cut(s) 88, 589, 709
BstMWI GCNNNNNNNGC 7 cut(s) 34, 43, 404, 550, 668, 692, 867
BstNI CCWGG 1 cut(s) 404
BstPI GGTNACC 1 cut(s) 77
BstSCI CCNGG 2 cut(s) 73, 402
BstSFI CTRYAG 1 cut(s) 264
BstSLI GKGCMC 1 cut(s) 506
BstV1I GCAGC 5 cut(s) 21, 188, 671, 688, 857
BstV2I GAAGAC 1 cut(s) 575
BstX2I RGATCY 1 cut(s) 589
BstXI CCANNNNNNTGG 2 cut(s) 21, 220
BstYI RGATCY 1 cut(s) 589
BtsCI GGATG 5 cut(s) 67, 209, 425, 440, 701
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 4 cut(s) 68, 451, 831, 838
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 4 cut(s) 106, 542, 868, 953
CfoI GCGC 1 cut(s) 809
CseI GACGC 1 cut(s) 646
Csp6I GTAC 2 cut(s) 217, 982
CviAII CATG 4 cut(s) 220, 716, 746, 894
CviQI GTAC 2 cut(s) 217, 982
DdeI CTNAG 4 cut(s) 145, 803, 823, 879
DpnI GATC 3 cut(s) 90, 591, 711
DpnII GATC 3 cut(s) 88, 589, 709
Eam1104I CTCTTC 2 cut(s) 414, 924
EarI CTCTTC 2 cut(s) 414, 924
Eco130I CCWWGG 1 cut(s) 381
Eco47III AGCGCT 1 cut(s) 808
Eco91I GGTNACC 1 cut(s) 77
EcoO65I GGTNACC 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 4 cut(s) 223, 719, 749, 897
FaqI GGGAC 1 cut(s) 482
FatI CATG 4 cut(s) 219, 715, 745, 893
Fnu4HI GCNGC 5 cut(s) 35, 202, 660, 677, 871
FokI GGATG 5 cut(s) 74, 196, 427, 432, 688
Fsp4HI GCNGC 5 cut(s) 35, 202, 660, 677, 871
FspBI CTAG 3 cut(s) 38, 587, 687
GlaI GCGC 1 cut(s) 808
GluI GCNGC 5 cut(s) 35, 202, 660, 677, 871
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 1 cut(s) 354
HaeII RGCGCY 1 cut(s) 810
HapII CCGG 2 cut(s) 74, 705
HgaI GACGC 1 cut(s) 646
HhaI GCGC 1 cut(s) 809
Hin1I GRCGYC 1 cut(s) 638
Hin1II CATG 4 cut(s) 223, 719, 749, 897
Hin6I GCGC 1 cut(s) 807
HinP1I GCGC 1 cut(s) 807
HpaII CCGG 2 cut(s) 74, 705
HphI GGTGA 1 cut(s) 719
Hpy188I TCNGA 3 cut(s) 173, 567, 880
Hpy188III TCNNGA 5 cut(s) 86, 147, 417, 790, 923
HpyCH4III ACNGT 2 cut(s) 314, 455
HpyF10VI GCNNNNNNNGC 7 cut(s) 34, 43, 404, 550, 668, 692, 867
HpyF3I CTNAG 4 cut(s) 145, 803, 823, 879
Hsp92I GRCGYC 1 cut(s) 638
Hsp92II CATG 4 cut(s) 223, 719, 749, 897
HspAI GCGC 1 cut(s) 807
Kzo9I GATC 3 cut(s) 88, 589, 709
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 1 cut(s) 684
Lsp1109I GCAGC 5 cut(s) 21, 188, 671, 688, 857
LweI GCATC 5 cut(s) 332, 449, 612, 658, 784
MaeI CTAG 3 cut(s) 38, 587, 687
MaeIII GTNAC 3 cut(s) 3, 77, 826
MalI GATC 3 cut(s) 90, 591, 711
MboI GATC 3 cut(s) 88, 589, 709
MboII GAAGA 4 cut(s) 274, 401, 580, 911
MfeI CAATTG 2 cut(s) 230, 956
MflI RGATCY 1 cut(s) 589
MhlI GDGCHC 1 cut(s) 506
MmeI TCCRAC 4 cut(s) 98, 503, 617, 952
MnlI CCTC 5 cut(s) 114, 143, 708, 723, 927
MroXI GAANNNNTTC 1 cut(s) 818
MseI TTAA 3 cut(s) 183, 512, 633
MslI CAYNNNNRTG 2 cut(s) 19, 456
MspA1I CMGCKG 2 cut(s) 104, 428
MspCI CTTAAG 1 cut(s) 511
MspI CCGG 2 cut(s) 74, 705
MspR9I CCNGG 2 cut(s) 75, 404
MunI CAATTG 2 cut(s) 230, 956
Mva1269I GAATGC 3 cut(s) 393, 400, 737
MvaI CCWGG 1 cut(s) 404
MwoI GCNNNNNNNGC 7 cut(s) 34, 43, 404, 550, 668, 692, 867
NciI CCSGG 1 cut(s) 75
NdeII GATC 3 cut(s) 88, 589, 709
NlaIII CATG 4 cut(s) 223, 719, 749, 897
NmuCI GTSAC 2 cut(s) 3, 826
OliI CACNNNNGTG 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 414
PctI GAATGC 3 cut(s) 393, 400, 737
PdmI GAANNNNTTC 1 cut(s) 818
PkrI GCNGC 5 cut(s) 36, 203, 661, 678, 872
PshBI ATTAAT 1 cut(s) 183
PsiI TTATAA 1 cut(s) 653
Psp6I CCWGG 1 cut(s) 402
PspEI GGTNACC 1 cut(s) 77
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 1 cut(s) 402
PstI CTGCAG 1 cut(s) 268
PsuI RGATCY 1 cut(s) 589
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 2 cut(s) 218, 983
RsaNI GTAC 2 cut(s) 217, 982
RseI CAYNNNNRTG 2 cut(s) 19, 456
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 3 cut(s) 183, 512, 633
SatI GCNGC 5 cut(s) 35, 202, 660, 677, 871
Sau3AI GATC 3 cut(s) 88, 589, 709
ScrFI CCNGG 2 cut(s) 75, 404
SduI GDGCHC 1 cut(s) 506
SfaNI GCATC 5 cut(s) 332, 449, 612, 658, 784
SfcI CTRYAG 1 cut(s) 264
SmiMI CAYNNNNRTG 2 cut(s) 19, 456
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
SsiI CCGC 2 cut(s) 204, 426
SspI AATATT 2 cut(s) 349, 562
SspMI CTAG 3 cut(s) 38, 587, 687
StyD4I CCNGG 2 cut(s) 73, 402
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 2 cut(s) 314, 455
TatI WGTACW 1 cut(s) 216
Tru1I TTAA 3 cut(s) 183, 512, 633
Tru9I TTAA 3 cut(s) 183, 512, 633
TscAI CASTG 4 cut(s) 75, 458, 831, 838
TseFI GTSAC 2 cut(s) 3, 826
TseI GCWGC 5 cut(s) 34, 201, 659, 676, 870
Tsp45I GTSAC 2 cut(s) 3, 826
TspDTI ATGAA 4 cut(s) 198, 744, 750, 838
TspRI CASTG 4 cut(s) 75, 458, 831, 838
Vha464I CTTAAG 1 cut(s) 511
VspI ATTAAT 1 cut(s) 183
XapI RAATTY 3 cut(s) 246, 517, 616
XmnI GAANNNNTTC 1 cut(s) 818
XspI CTAG 3 cut(s) 38, 587, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.