AT3G62060

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002705
Physical Location & Seq
Forward (+)
22979848 .. 22983250
3403 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G62060.2

Sequence Viewer

Length: 1260 bp
ATGAGGAGCTTGTTGTTGTGGATTGCGGTGGTCGTGTGCTTGCTATGTTCAGTCGCGGCGGTGGTTCAAAGCGGATCTAGCGACGGATTTGGGAAACCCAGAGATACTGAGACTGCGATATCTTTTCTGGAGTATAAATTGATGGCGCCTTCGGTTCCAATGATACCTCTCACCTTGATTCACGGAGCTGACTCTAAAGGAGCTGTGTGCTTAGATGGGACGCTACCTGGTTATCATCTAGATCGTGGTTTTGGATCAGGTGCTAACAGTTGGCTTATCCAGCTTGAGGGTGGAGGATGGTGTAACAACCATAGGAGCTGTGTGTATCGCAAAACAAGTCGACGTGGTTCCTCTAAATTCATGGAAAAAGCTTTGGCCTTCACTGGAATTTTGAGCAATAGATCTGAAGAGAATCCTGACTTCTTTAATTGGAACAGGATCAAGTTGCGTTACTGCGATGGTGCGTCTTTCTCTGGTGATAGTCAGGACGAGAGTTCGCAACTTTTCTATCGAGGACAACGAATCTGGCAAGTGGCTATGGAAGAATTCCTGTCTTTAGGCATGAAGCAAGCAAACCAGGCTCTGCTCTCTGGATGTTCAGCTGGAGGATTAGCTTCTATTTTGCACTGTGATGAGTTCAGGGAACTATTACCTAGTTCCACGAAAGTAAAATGCTTAAGTGATGCTGGAATGTTTCTGGACTCAGTGGATGTCTCTGGGGGCCACTCGCTTAGGAATATGTTCCAAGGTGTTGTTACAGTGCAGAACCTCCAAAAGGACTTGTCCAGTACTTGTACAAACCATTTGGATCCTACTTCGTGCTTTTTTCCCCAGAACTTGGTTTCAGACATTAAAACCCCAATGTTTCTTCTCAACACAGCATATGACTCTTGGCAGATCCAAGAGAGCTTAGCTCCTCCTACTGCTGATCCAGGCGGTATCTGGAAGGCATGCAAATCAGATCACTCGCGATGTAATTCATCACAGATCCAATTCTTCCAAGAATTCAGGAATCAAATGCTGTTTGCTGTAAACTCATTCTCAAACTCTGACCAGAACGGTCTTTATATAAACTCTTGCTTCGCGCATTGTCAGACTGAAAGACAGGACACTTGGTTTGCTCAAGATTCTCCTCAACTAAACGGAAAGAGAGTCGCAGAGTCTGTAGGTGACTGGTACTTCGACCGAGCAAAAAATGTCAAAGCTATCGATTGTCCTTACCCTTGTGACACAACATGTCACAATCTGATTTTCGAGTGA

Protein Analysis

419

Amino Acids

46.48

Weight (kDa)

5.87

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 54 - 414 1.9e-169 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 145
AccB7I CCANNNNNTGG 1 cut(s) 838
AccI GTMKAC 1 cut(s) 340
AccII CGCG 3 cut(s) 56, 970, 1085
AciI CCGC 5 cut(s) 26, 56, 59, 72, 936
AclWI GGATC 8 cut(s) 82, 262, 446, 803, 816, 892, 923, 982
AcsI RAATTY 4 cut(s) 356, 387, 545, 1004
AcuI CTGAAG 1 cut(s) 426
AcyI GRCGYC 1 cut(s) 146
AfaI GTAC 3 cut(s) 790, 796, 1178
AfiI CCNNNNNNNGG 3 cut(s) 286, 775, 838
AflII CTTAAG 1 cut(s) 676
AflIII ACRYGT 1 cut(s) 1235
AgsI TTSAA 1 cut(s) 68
AjiI CACGTC 1 cut(s) 344
AjnI CCWGG 3 cut(s) 226, 576, 931
Alw26I GTCTC 2 cut(s) 104, 718
AlwI GGATC 8 cut(s) 82, 262, 446, 803, 816, 892, 923, 982
AlwNI CAGNNNCTG 2 cut(s) 583, 1163
AoxI GGCC 2 cut(s) 375, 721
ApoI RAATTY 4 cut(s) 356, 387, 545, 1004
ArsI GACNNNNNNTTYG 4 cut(s) 1100, 1132, 1163, 1195
Asp700I GAANNNNTTC 1 cut(s) 740
AspLEI GCGC 2 cut(s) 148, 1087
AspS9I GGNCC 1 cut(s) 721
AsuHPI GGTGA 3 cut(s) 163, 488, 1181
BamHI GGATCC 1 cut(s) 808
BanI GGYRCC 1 cut(s) 145
BccI CCATC 4 cut(s) 136, 209, 291, 452
BciT130I CCWGG 3 cut(s) 228, 578, 933
BcoDI GTCTC 2 cut(s) 104, 718
BfaI CTAG 3 cut(s) 78, 239, 654
BfmI CTRYAG 1 cut(s) 1164
BfoI RGCGCY 1 cut(s) 149
BfrI CTTAAG 1 cut(s) 676
BglII AGATCT 1 cut(s) 401
BisI GCNGC 1 cut(s) 57
BlpI GCTNAGC 1 cut(s) 910
BlsI GCNGC 1 cut(s) 58
BmcAI AGTACT 1 cut(s) 790
Bme1390I CCNGG 3 cut(s) 228, 578, 933
BmgBI CACGTC 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 721
BmiI GGNNCC 5 cut(s) 147, 156, 349, 722, 810
BmrFI CCNGG 3 cut(s) 228, 578, 933
BmsI GCATC 1 cut(s) 673
BplI GAGNNNNNCTC 2 cut(s) 898, 930
BpmI CTGGAG 2 cut(s) 149, 624
Bpu10I CCTNAGC 1 cut(s) 731
Bpu1102I GCTNAGC 1 cut(s) 910
BpuEI CTTGAG 2 cut(s) 305, 1107
Bsa29I ATCGAT 1 cut(s) 1209
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 745
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 775, 838
Bse1I ACTGG 3 cut(s) 388, 786, 1178
BseBI CCWGG 3 cut(s) 228, 578, 933
BseCI ATCGAT 1 cut(s) 1209
BseDI CCNNGG 1 cut(s) 745
BseGI GGATG 3 cut(s) 302, 599, 715
BseLI CCNNNNNNNGG 3 cut(s) 286, 775, 838
BseMII CTCAG 2 cut(s) 99, 717
BseNI ACTGG 3 cut(s) 388, 786, 1178
BseRI GAGGAG 3 cut(s) 19, 906, 1122
BsgI GTGCAG 1 cut(s) 782
Bsh1236I CGCG 3 cut(s) 56, 970, 1085
Bsh1285I CGRYCG 1 cut(s) 1186
BshFI GGCC 2 cut(s) 377, 723
BshNI GGYRCC 1 cut(s) 145
BshVI ATCGAT 1 cut(s) 1209
BsiEI CGRYCG 1 cut(s) 1186
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 3 cut(s) 286, 775, 838
BsmAI GTCTC 2 cut(s) 104, 718
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 2 cut(s) 377, 723
Bsp1407I TGTACA 1 cut(s) 794
Bsp1720I GCTNAGC 1 cut(s) 910
Bsp68I TCGCGA 1 cut(s) 970
BspACI CCGC 5 cut(s) 26, 56, 59, 72, 936
BspANI GGCC 2 cut(s) 377, 723
BspCNI CTCAG 2 cut(s) 100, 716
BspDI ATCGAT 1 cut(s) 1209
BspFNI CGCG 3 cut(s) 56, 970, 1085
BspLI GGNNCC 5 cut(s) 147, 156, 349, 722, 810
BspPI GGATC 8 cut(s) 82, 262, 446, 803, 816, 892, 923, 982
BspT107I GGYRCC 1 cut(s) 145
BspTI CTTAAG 1 cut(s) 676
BsrGI TGTACA 1 cut(s) 794
BsrI ACTGG 3 cut(s) 388, 786, 1178
BssECI CCNNGG 1 cut(s) 745
BssNI GRCGYC 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 745
Bst2UI CCWGG 3 cut(s) 228, 578, 933
Bst4CI ACNGT 4 cut(s) 269, 629, 760, 1061
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 1 cut(s) 146
BstAFI CTTAAG 1 cut(s) 676
BstAUI TGTACA 1 cut(s) 794
BstC8I GCNNGC 3 cut(s) 41, 570, 952
BstDEI CTNAG 5 cut(s) 108, 211, 703, 731, 910
BstENI CCTNNNNNAGG 1 cut(s) 773
BstF5I GGATG 3 cut(s) 302, 599, 715
BstFNI CGCG 3 cut(s) 56, 970, 1085
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 2 cut(s) 148, 1087
BstMAI GTCTC 2 cut(s) 104, 718
BstMCI CGRYCG 1 cut(s) 1186
BstMWI GCNNNNNNNGC 3 cut(s) 78, 280, 578
BstNI CCWGG 3 cut(s) 228, 578, 933
BstNSI RCATGY 2 cut(s) 954, 1239
BstSCI CCNGG 3 cut(s) 226, 576, 931
BstSFI CTRYAG 1 cut(s) 1164
BstUI CGCG 3 cut(s) 56, 970, 1085
BstX2I RGATCY 5 cut(s) 74, 401, 808, 897, 987
BstYI RGATCY 5 cut(s) 74, 401, 808, 897, 987
Bsu15I ATCGAT 1 cut(s) 1209
BsuRI GGCC 2 cut(s) 377, 723
BsuTUI ATCGAT 1 cut(s) 1209
BtgZI GCGATG 2 cut(s) 471, 985
BtrI CACGTC 1 cut(s) 344
BtsCI GGATG 3 cut(s) 302, 599, 715
BtsIMutI CAGTG 4 cut(s) 381, 625, 711, 765
BtuMI TCGCGA 1 cut(s) 970
Cac8I GCNNGC 3 cut(s) 41, 570, 952
CaiI CAGNNNCTG 2 cut(s) 583, 1163
CfoI GCGC 2 cut(s) 148, 1087
Cfr13I GGNCC 1 cut(s) 721
ClaI ATCGAT 1 cut(s) 1209
CseI GACGC 2 cut(s) 229, 453
CsiI ACCWGGT 1 cut(s) 226
Csp6I GTAC 3 cut(s) 789, 795, 1177
CviAII CATG 4 cut(s) 361, 562, 951, 1236
CviQI GTAC 3 cut(s) 789, 795, 1177
DdeI CTNAG 5 cut(s) 108, 211, 703, 731, 910
DinI GGCGCC 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Eco130I CCWWGG 1 cut(s) 745
Eco32I GATATC 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 426
EcoNI CCTNNNNNAGG 1 cut(s) 773
EcoRI GAATTC 2 cut(s) 545, 1004
EcoRII CCWGG 3 cut(s) 226, 576, 931
EcoRV GATATC 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 745
EgeI GGCGCC 1 cut(s) 147
EheI GGCGCC 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 745
FaeI CATG 4 cut(s) 364, 565, 954, 1239
FaqI GGGAC 1 cut(s) 232
FatI CATG 4 cut(s) 360, 561, 950, 1235
FauNDI CATATG 1 cut(s) 883
FblI GTMKAC 1 cut(s) 340
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 3 cut(s) 309, 606, 722
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 3 cut(s) 78, 239, 654
GlaI GCGC 2 cut(s) 147, 1086
GluI GCNGC 1 cut(s) 57
GsuI CTGGAG 2 cut(s) 149, 624
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 2 cut(s) 377, 723
HgaI GACGC 2 cut(s) 229, 453
HhaI GCGC 2 cut(s) 148, 1087
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 4 cut(s) 364, 565, 954, 1239
Hin6I GCGC 2 cut(s) 146, 1085
HinP1I GCGC 2 cut(s) 146, 1085
HincII GTYRAC 1 cut(s) 341
HindII GTYRAC 1 cut(s) 341
HindIII AAGCTT 1 cut(s) 369
HphI GGTGA 3 cut(s) 163, 488, 1181
Hpy166II GTNNAC 2 cut(s) 341, 1033
Hpy188I TCNGA 6 cut(s) 406, 847, 961, 1051, 1095, 1248
Hpy8I GTNNAC 2 cut(s) 341, 1033
Hpy99I CGWCG 2 cut(s) 86, 345
HpyAV CCTTC 3 cut(s) 159, 388, 940
HpyCH4III ACNGT 4 cut(s) 269, 629, 760, 1061
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 3 cut(s) 625, 763, 954
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 280, 578
HpyF3I CTNAG 5 cut(s) 108, 211, 703, 731, 910
HpySE526I ACGT 1 cut(s) 343
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 4 cut(s) 364, 565, 954, 1239
HspAI GCGC 2 cut(s) 146, 1085
KasI GGCGCC 1 cut(s) 145
LmnI GCTCC 5 cut(s) 6, 185, 200, 315, 919
LweI GCATC 1 cut(s) 673
MabI ACCWGGT 1 cut(s) 226
MaeI CTAG 3 cut(s) 78, 239, 654
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 6 cut(s) 302, 449, 754, 1169, 1226, 1238
MboII GAAGA 4 cut(s) 419, 554, 860, 988
MflI RGATCY 5 cut(s) 74, 401, 808, 897, 987
MluCI AATT 8 cut(s) 137, 356, 387, 427, 545, 976, 992, 1004
Mly113I GGCGCC 1 cut(s) 146
MlyI GAGTC 5 cut(s) 185, 695, 881, 1161, 1169
MnlI CCTC 9 cut(s) 177, 280, 287, 361, 506, 599, 779, 927, 1143
MroXI GAANNNNTTC 1 cut(s) 740
MseI TTAA 3 cut(s) 426, 677, 852
MslI CAYNNNNRTG 1 cut(s) 630
MspA1I CMGCKG 1 cut(s) 602
MspCI CTTAAG 1 cut(s) 676
MspR9I CCNGG 3 cut(s) 228, 578, 933
MvaI CCWGG 3 cut(s) 228, 578, 933
MvnI CGCG 3 cut(s) 56, 970, 1085
MwoI GCNNNNNNNGC 3 cut(s) 78, 280, 578
NarI GGCGCC 1 cut(s) 146
NdeI CATATG 1 cut(s) 883
NlaIII CATG 4 cut(s) 364, 565, 954, 1239
NlaIV GGNNCC 5 cut(s) 147, 156, 349, 722, 810
NmuCI GTSAC 3 cut(s) 1169, 1226, 1238
NruI TCGCGA 1 cut(s) 970
NspI RCATGY 2 cut(s) 954, 1239
PaeI GCATGC 1 cut(s) 954
PciI ACATGT 1 cut(s) 1235
PcsI WCGNNNNNNNCGW 1 cut(s) 517
PdmI GAANNNNTTC 1 cut(s) 740
PfeI GAWTC 5 cut(s) 178, 412, 522, 1012, 1127
PflMI CCANNNNNTGG 1 cut(s) 838
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 5 cut(s) 185, 695, 881, 1160, 1168
PluTI GGCGCC 1 cut(s) 149
PpsI GAGTC 5 cut(s) 185, 695, 881, 1160, 1168
PscI ACATGT 1 cut(s) 1235
Psp6I CCWGG 3 cut(s) 226, 576, 931
PspGI CCWGG 3 cut(s) 226, 576, 931
PspN4I GGNNCC 5 cut(s) 147, 156, 349, 722, 810
PspPI GGNCC 1 cut(s) 721
PstNI CAGNNNCTG 2 cut(s) 583, 1163
PsuI RGATCY 5 cut(s) 74, 401, 808, 897, 987
PvuII CAGCTG 1 cut(s) 602
RruI TCGCGA 1 cut(s) 970
RsaI GTAC 3 cut(s) 790, 796, 1178
RsaNI GTAC 3 cut(s) 789, 795, 1177
RseI CAYNNNNRTG 1 cut(s) 630
SalI GTCGAC 1 cut(s) 339
SaqAI TTAA 3 cut(s) 426, 677, 852
SatI GCNGC 1 cut(s) 57
Sau96I GGNCC 1 cut(s) 721
ScaI AGTACT 1 cut(s) 790
SchI GAGTC 5 cut(s) 185, 695, 881, 1161, 1169
ScrFI CCNGG 3 cut(s) 228, 578, 933
SexAI ACCWGGT 1 cut(s) 226
SfaNI GCATC 1 cut(s) 673
SfcI CTRYAG 1 cut(s) 1164
SfoI GGCGCC 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 630
SmlI CTYRAG 3 cut(s) 284, 676, 1122
SmoI CTYRAG 3 cut(s) 284, 676, 1122
SphI GCATGC 1 cut(s) 954
Sse9I AATT 8 cut(s) 137, 356, 387, 427, 545, 976, 992, 1004
SsiI CCGC 5 cut(s) 26, 56, 59, 72, 936
SspDI GGCGCC 1 cut(s) 145
SspMI CTAG 3 cut(s) 78, 239, 654
StyD4I CCNGG 3 cut(s) 226, 576, 931
StyI CCWWGG 1 cut(s) 745
TaaI ACNGT 4 cut(s) 269, 629, 760, 1061
TaiI ACGT 1 cut(s) 346
TaqI TCGA 5 cut(s) 340, 511, 1182, 1209, 1254
TaqII GACCGA 1 cut(s) 1200
TasI AATT 8 cut(s) 137, 356, 387, 427, 545, 976, 992, 1004
TatI WGTACW 2 cut(s) 788, 794
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 5 cut(s) 178, 412, 522, 1012, 1127
Tru1I TTAA 3 cut(s) 426, 677, 852
Tru9I TTAA 3 cut(s) 426, 677, 852
TscAI CASTG 4 cut(s) 388, 632, 711, 765
TseFI GTSAC 3 cut(s) 1169, 1226, 1238
Tsp45I GTSAC 3 cut(s) 1169, 1226, 1238
TspDTI ATGAA 3 cut(s) 349, 578, 969
TspGWI ACGGA 3 cut(s) 99, 198, 1158
TspRI CASTG 4 cut(s) 388, 632, 711, 765
Van91I CCANNNNNTGG 1 cut(s) 838
Vha464I CTTAAG 1 cut(s) 676
XagI CCTNNNNNAGG 1 cut(s) 773
XapI RAATTY 4 cut(s) 356, 387, 545, 1004
XbaI TCTAGA 1 cut(s) 238
XceI RCATGY 2 cut(s) 954, 1239
XcmI CCANNNNNNNNNTGG 2 cut(s) 287, 939
XmiI GTMKAC 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 740
XspI CTAG 3 cut(s) 78, 239, 654
ZrmI AGTACT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.