Rw3G023980

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002705
Physical Location & Seq
Forward (+)
30535230 .. 30540285
5056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G023980.1

Sequence Viewer

Length: 1431 bp
ATGAAGAAGCAGCAGCAGCAGCTGAATGTGGGTACAGTGTTGGTGGTGGCATTGGCAGCTGCCTTTGTCAATCTGAAACTCAATGTGGGTGCTTTGAGTTTGAACGAATCAGTGAACGTTGGGAGAAAAGATGCAGTCTTGTTCGACAACGATCATGTCTCAGCCTCAGCCTCAGCCTCACCGACACAGCCTCAGCCTCTCATGGTGGCCCTCACTCTTATCGACAGCGCTGCTTCTAAAGGAGCTGTCTGTTTGGATGGAACACCGCCCGGTTACCATCTGGATCGTGGGTCTGGATCCGGTGCAAACAGCTGGCTCATTCAGTTGGAGGGAGGTGGGTGGTGTAATACTATCAGAAATTGTGTTTATCGCAAGACCACTCGCCGCGGTTCATCCAACCACATGGAAAAGCAATTACCATTCACAGGAATTTTGAGCAATAAACCTGAAGAAAACCCCGACTTCTTCAACTGGAACAGGGTCAAGCTACGATACTGTGATGGGGCATCTTTTGCCGGAGACAGCCAGAATGAGGCTGCACAACTTAATTTTCGAGGGCAAAAGATTTGGTTGGTTGCTATGGAAGAGTTGATGTCTAAGGGAATGCAGAATGCTAACCAGGCTCTTCTTTCTGGATGTTCCGCTGGGGGTCTGGCATCCATACTACACTGTGATGAGTTCCGGGACTTGTTTCCAACCAGTACCACAGTGAAATGTCTGAGTGATGCTGGGATGTTCCTTGATGCAATTGATGTATCTGGTGGGCACACTCTAAGGAATTTGTATGCAGGAGTAGTTAGCTTGCAGGAAGTGCAAAAGAATCTTCCAAAGACCTGTATCAACCACCTAGATCCAACTTCGTGCTTTTTTCCTCAGAATTTGGTCGCAAATGTTAAAACGCCAATGTTTCTTCTAAATGCAGCCTATGATGCTTGGCAGCTCCAAGCTAGTTTAGCGCCTCCATCTGCTGATCCTAATGGTTTATGGAATGAATGCAAATCAAACCATGCAAATTGTAATTTATCCCAGATCCAGTTTCTCCAAGATTTCAGGAATCAAATGCTCAGTGCTGTGAAAGATTTCTCAATGTCCCGTGAAAATGGTTTATTCATAAACTCTTGCTTTGCTCACTGCCAGTCTGAGAGACAAGATACATGGTTTGCTGATGATTCTCCTGTTCTTGAAAACCAGCCAATTGCACTGTCTGTTGGAGACTGGTACTTTGATCGAAGGACAGTTAAAGCTATTGACTGTGAATACCCCTGTGATAGCAGCTGTCACAATCTTAACTTCAAAGAGGATGTAACTTCTACGACATTGACATATTCTCAGTCTGCAAAGTCGTCTATTACCATGCTAAATCTGCTAGGTGCCTTGTTGATGCCTATAACGTGTTCGAGATGCATAACGTGGTTTTCATTTGTCCACTGA

Protein Analysis

476

Amino Acids

52.23

Weight (kDa)

5.94

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 64 - 427 1.3e-173 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1370
AccII CGCG 1 cut(s) 387
AciI CCGC 4 cut(s) 266, 385, 387, 642
AclI AACGTT 1 cut(s) 117
AclWI GGATC 6 cut(s) 291, 291, 304, 845, 965, 1024
AcsI RAATTY 3 cut(s) 429, 778, 877
AcuI CTGAAG 1 cut(s) 468
AfaI GTAC 3 cut(s) 34, 703, 1220
AfeI AGCGCT 1 cut(s) 229
AfiI CCNNNNNNNGG 2 cut(s) 425, 532
AflIII ACRYGT 1 cut(s) 1391
AgsI TTSAA 4 cut(s) 103, 469, 1184, 1294
AjnI CCWGG 1 cut(s) 618
Alw26I GTCTC 4 cut(s) 163, 513, 1138, 1206
AlwI GGATC 6 cut(s) 291, 291, 304, 845, 965, 1024
AlwNI CAGNNNCTG 1 cut(s) 22
Aor51HI AGCGCT 1 cut(s) 229
AoxI GGCC 1 cut(s) 207
ApoI RAATTY 3 cut(s) 429, 778, 877
ArsI GACNNNNNNTTYG 2 cut(s) 1205, 1237
Asp700I GAANNNNTTC 1 cut(s) 1079
AspLEI GCGC 2 cut(s) 230, 958
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 2 cut(s) 270, 683
AsuHPI GGTGA 1 cut(s) 171
BaeGI GKGCMC 1 cut(s) 768
BamHI GGATCC 1 cut(s) 296
BanI GGYRCC 1 cut(s) 1370
BbvCI CCTCAGC 3 cut(s) 166, 172, 192
BccI CCATC 4 cut(s) 251, 285, 494, 970
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BciT130I CCWGG 1 cut(s) 620
BcnI CCSGG 2 cut(s) 270, 683
BcoDI GTCTC 4 cut(s) 163, 513, 1138, 1206
BfaI CTAG 3 cut(s) 848, 948, 1367
BfoI RGCGCY 2 cut(s) 231, 959
Bme1390I CCNGG 3 cut(s) 270, 620, 683
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 2 cut(s) 298, 1372
BmrFI CCNGG 3 cut(s) 270, 620, 683
BmsI GCATC 8 cut(s) 121, 515, 665, 715, 733, 919, 1371, 1391
Bpu10I CCTNAGC 3 cut(s) 166, 172, 192
BpuMI CCSGG 2 cut(s) 270, 683
BsaJI CCNNGG 1 cut(s) 385
BsaWI WCCGGW 1 cut(s) 299
Bsc4I CCNNNNNNNGG 2 cut(s) 425, 532
Bse1I ACTGG 5 cut(s) 476, 699, 1033, 1135, 1220
BseBI CCWGG 1 cut(s) 620
BseDI CCNNGG 1 cut(s) 385
BseGI GGATG 6 cut(s) 262, 392, 641, 656, 738, 1306
BseLI CCNNNNNNNGG 2 cut(s) 425, 532
BseMII CTCAG 9 cut(s) 174, 180, 186, 206, 710, 887, 1078, 1131, 1343
BseNI ACTGG 5 cut(s) 476, 699, 1033, 1135, 1220
BseSI GKGCMC 1 cut(s) 768
BseYI CCCAGC 2 cut(s) 644, 728
BsgI GTGCAG 1 cut(s) 522
Bsh1236I CGCG 1 cut(s) 387
BshFI GGCC 1 cut(s) 209
BshNI GGYRCC 1 cut(s) 1370
BsiSI CCGG 4 cut(s) 270, 300, 516, 682
BslFI GGGAC 2 cut(s) 698, 1075
BslI CCNNNNNNNGG 2 cut(s) 425, 532
BsmAI GTCTC 4 cut(s) 163, 513, 1138, 1206
BsmFI GGGAC 2 cut(s) 698, 1075
BsmI GAATGC 3 cut(s) 609, 616, 998
BsnI GGCC 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 768
Bsp143I GATC 7 cut(s) 151, 283, 296, 850, 970, 1029, 1225
BspACI CCGC 4 cut(s) 266, 385, 387, 642
BspANI GGCC 1 cut(s) 209
BspCNI CTCAG 9 cut(s) 173, 179, 185, 205, 711, 886, 1077, 1132, 1342
BspFNI CGCG 1 cut(s) 387
BspLI GGNNCC 2 cut(s) 298, 1372
BspPI GGATC 6 cut(s) 291, 291, 304, 845, 965, 1024
BspQI GCTCTTC 1 cut(s) 630
BspT107I GGYRCC 1 cut(s) 1370
BsrI ACTGG 5 cut(s) 476, 699, 1033, 1135, 1220
BssECI CCNNGG 1 cut(s) 385
BssMI GATC 7 cut(s) 151, 283, 296, 850, 970, 1029, 1225
Bst2UI CCWGG 1 cut(s) 620
Bst4CI ACNGT 7 cut(s) 37, 497, 671, 709, 1203, 1237, 1253
Bst6I CTCTTC 2 cut(s) 579, 630
BstAPI GCANNNNNTGC 2 cut(s) 512, 811
BstC8I GCNNGC 2 cut(s) 314, 803
BstDSI CCRYGG 1 cut(s) 385
BstEII GGTNACC 1 cut(s) 272
BstF5I GGATG 6 cut(s) 262, 392, 641, 656, 738, 1306
BstFNI CGCG 1 cut(s) 387
BstH2I RGCGCY 2 cut(s) 231, 959
BstHHI GCGC 2 cut(s) 230, 958
BstKTI GATC 7 cut(s) 154, 286, 299, 853, 973, 1032, 1228
BstMAI GTCTC 4 cut(s) 163, 513, 1138, 1206
BstMBI GATC 7 cut(s) 151, 283, 296, 850, 970, 1029, 1225
BstMWI GCNNNNNNNGC 9 cut(s) 16, 19, 56, 512, 620, 811, 929, 953, 1363
BstNI CCWGG 1 cut(s) 620
BstPI GGTNACC 1 cut(s) 272
BstSCI CCNGG 3 cut(s) 268, 618, 681
BstSLI GKGCMC 1 cut(s) 768
BstUI CGCG 1 cut(s) 387
BstX2I RGATCY 3 cut(s) 296, 850, 1029
BstXI CCANNNNNNTGG 1 cut(s) 403
BstYI RGATCY 3 cut(s) 296, 850, 1029
BsuRI GGCC 1 cut(s) 209
BtgI CCRYGG 1 cut(s) 385
BtsCI GGATG 6 cut(s) 262, 392, 641, 656, 738, 1306
BtsI GCAGTG 1 cut(s) 1129
BtsIMutI CAGTG 8 cut(s) 42, 117, 667, 714, 1072, 1129, 1199, 1426
Cac8I GCNNGC 2 cut(s) 314, 803
CaiI CAGNNNCTG 1 cut(s) 22
CfoI GCGC 2 cut(s) 230, 958
Cfr13I GGNCC 1 cut(s) 208
Cfr42I CCGCGG 1 cut(s) 388
Csp6I GTAC 3 cut(s) 33, 702, 1219
CviAII CATG 6 cut(s) 155, 202, 403, 1007, 1155, 1354
CviQI GTAC 3 cut(s) 33, 702, 1219
DpnI GATC 7 cut(s) 153, 285, 298, 852, 972, 1031, 1227
DpnII GATC 7 cut(s) 151, 283, 296, 850, 970, 1029, 1225
Eam1104I CTCTTC 2 cut(s) 579, 630
EarI CTCTTC 2 cut(s) 579, 630
Eco47III AGCGCT 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 468
Eco91I GGTNACC 1 cut(s) 272
EcoO65I GGTNACC 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 618
EcoT22I ATGCAT 1 cut(s) 1406
FaeI CATG 6 cut(s) 158, 205, 406, 1010, 1158, 1357
FaqI GGGAC 2 cut(s) 698, 1075
FatI CATG 6 cut(s) 154, 201, 402, 1006, 1154, 1353
FokI GGATG 6 cut(s) 269, 379, 643, 648, 745, 1313
FspBI CTAG 3 cut(s) 848, 948, 1367
GlaI GCGC 2 cut(s) 229, 957
GsaI CCCAGC 2 cut(s) 648, 732
HaeII RGCGCY 2 cut(s) 231, 959
HaeIII GGCC 1 cut(s) 209
HapII CCGG 4 cut(s) 270, 300, 516, 682
HhaI GCGC 2 cut(s) 230, 958
Hin1II CATG 6 cut(s) 158, 205, 406, 1010, 1158, 1357
Hin6I GCGC 2 cut(s) 228, 956
HinP1I GCGC 2 cut(s) 228, 956
HinfI GANTC 4 cut(s) 107, 820, 1054, 1169
HpaII CCGG 4 cut(s) 270, 300, 516, 682
HphI GGTGA 1 cut(s) 171
Hpy166II GTNNAC 2 cut(s) 115, 1426
Hpy188I TCNGA 5 cut(s) 75, 356, 720, 876, 1141
Hpy188III TCNNGA 6 cut(s) 281, 294, 633, 1051, 1181, 1398
Hpy8I GTNNAC 2 cut(s) 115, 1426
HpyAV CCTTC 1 cut(s) 1224
HpyCH4III ACNGT 7 cut(s) 37, 497, 671, 709, 1203, 1237, 1253
HpyCH4IV ACGT 3 cut(s) 117, 1391, 1409
HpyF10VI GCNNNNNNNGC 9 cut(s) 16, 19, 56, 512, 620, 811, 929, 953, 1363
HpySE526I ACGT 3 cut(s) 117, 1391, 1409
Hsp92II CATG 6 cut(s) 158, 205, 406, 1010, 1158, 1357
HspAI GCGC 2 cut(s) 228, 956
KspI CCGCGG 1 cut(s) 388
Kzo9I GATC 7 cut(s) 151, 283, 296, 850, 970, 1029, 1225
LguI GCTCTTC 1 cut(s) 630
LmnI GCTCC 2 cut(s) 242, 945
LweI GCATC 8 cut(s) 121, 515, 665, 715, 733, 919, 1371, 1391
MaeI CTAG 3 cut(s) 848, 948, 1367
MaeII ACGT 3 cut(s) 117, 1391, 1409
MaeIII GTNAC 3 cut(s) 272, 1277, 1303
MalI GATC 7 cut(s) 153, 285, 298, 852, 972, 1031, 1227
MboI GATC 7 cut(s) 151, 283, 296, 850, 970, 1029, 1225
MboII GAAGA 7 cut(s) 16, 457, 461, 596, 617, 815, 902
MfeI CAATTG 2 cut(s) 747, 1194
MflI RGATCY 3 cut(s) 296, 850, 1029
MhlI GDGCHC 1 cut(s) 768
MmeI TCCRAC 5 cut(s) 306, 420, 719, 878, 1189
Mph1103I ATGCAT 1 cut(s) 1406
MroXI GAANNNNTTC 1 cut(s) 1079
MseI TTAA 4 cut(s) 546, 894, 1239, 1287
MslI CAYNNNNRTG 1 cut(s) 672
MspA1I CMGCKG 6 cut(s) 22, 59, 312, 387, 644, 1275
MspI CCGG 4 cut(s) 270, 300, 516, 682
MspR9I CCNGG 3 cut(s) 270, 620, 683
MunI CAATTG 2 cut(s) 747, 1194
Mva1269I GAATGC 3 cut(s) 609, 616, 998
MvaI CCWGG 1 cut(s) 620
MvnI CGCG 1 cut(s) 387
MwoI GCNNNNNNNGC 9 cut(s) 16, 19, 56, 512, 620, 811, 929, 953, 1363
NciI CCSGG 2 cut(s) 270, 683
NdeII GATC 7 cut(s) 151, 283, 296, 850, 970, 1029, 1225
NlaIII CATG 6 cut(s) 158, 205, 406, 1010, 1158, 1357
NlaIV GGNNCC 2 cut(s) 298, 1372
NmuCI GTSAC 1 cut(s) 1277
NsiI ATGCAT 1 cut(s) 1406
PciSI GCTCTTC 1 cut(s) 630
PctI GAATGC 3 cut(s) 609, 616, 998
PdmI GAANNNNTTC 1 cut(s) 1079
PfeI GAWTC 4 cut(s) 107, 820, 1054, 1169
PfoI TCCNGGA 1 cut(s) 681
Psp1406I AACGTT 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 618
PspEI GGTNACC 1 cut(s) 272
PspFI CCCAGC 2 cut(s) 644, 728
PspGI CCWGG 1 cut(s) 618
PspN4I GGNNCC 2 cut(s) 298, 1372
PspPI GGNCC 1 cut(s) 208
PstNI CAGNNNCTG 1 cut(s) 22
PsuI RGATCY 3 cut(s) 296, 850, 1029
PvuII CAGCTG 4 cut(s) 22, 59, 312, 1275
RsaI GTAC 3 cut(s) 34, 703, 1220
RsaNI GTAC 3 cut(s) 33, 702, 1219
RseI CAYNNNNRTG 1 cut(s) 672
SacII CCGCGG 1 cut(s) 388
SapI GCTCTTC 1 cut(s) 630
SaqAI TTAA 4 cut(s) 546, 894, 1239, 1287
Sau3AI GATC 7 cut(s) 151, 283, 296, 850, 970, 1029, 1225
Sau96I GGNCC 1 cut(s) 208
ScrFI CCNGG 3 cut(s) 270, 620, 683
SduI GDGCHC 1 cut(s) 768
SfaNI GCATC 8 cut(s) 121, 515, 665, 715, 733, 919, 1371, 1391
Sfr303I CCGCGG 1 cut(s) 388
SgrBI CCGCGG 1 cut(s) 388
SmiMI CAYNNNNRTG 1 cut(s) 672
SsiI CCGC 4 cut(s) 266, 385, 387, 642
SspMI CTAG 3 cut(s) 848, 948, 1367
StyD4I CCNGG 3 cut(s) 268, 618, 681
TaaI ACNGT 7 cut(s) 37, 497, 671, 709, 1203, 1237, 1253
TaiI ACGT 3 cut(s) 120, 1394, 1412
TaqI TCGA 5 cut(s) 144, 222, 553, 1228, 1397
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 4 cut(s) 107, 820, 1054, 1169
Tru1I TTAA 4 cut(s) 546, 894, 1239, 1287
Tru9I TTAA 4 cut(s) 546, 894, 1239, 1287
TscAI CASTG 7 cut(s) 42, 117, 674, 714, 1072, 1136, 1206
TseFI GTSAC 1 cut(s) 1277
Tsp45I GTSAC 1 cut(s) 1277
TspDTI ATGAA 5 cut(s) 17, 381, 1005, 1099, 1407
TspRI CASTG 7 cut(s) 42, 117, 674, 714, 1072, 1136, 1206
XapI RAATTY 3 cut(s) 429, 778, 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
XmnI GAANNNNTTC 1 cut(s) 1079
XspI CTAG 3 cut(s) 848, 948, 1367
Zsp2I ATGCAT 1 cut(s) 1406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.