Rroxscaffold_6G00396580

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002705
Physical Location & Seq
Reverse (-)
18096827 .. 18102252
5426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00396580.1

Sequence Viewer

Length: 1410 bp
ATGAAGCTGAATGTGGGTACAGTGTTGGTGGTGGCATTGGCAGCTGTCTTTGTCAATCTGAAACTCAATGTGGGTGCTTTGAGTTTGAACGAATCAGTGAACGTTGAGAGAAAAGATGCAGTCTTGTTCGACAACGATCATGTCTTGGCCTCAGCCTCACCGACACAGCCTCAGCCTCTCATGGTGGCCCTCACTCTTATCGACAGCGCTGCTTCTAAAGGAGCTGTCTGTTTGGATGGAACGCCGCCCGGTTACCATCTGGATCGTGGGTCTGGATCCGGTGCAAACAGCTGGCTCATTCAGTTGGAGGGAGGTGGGTGGTGCAATACTATCAGAAATTGTGTTTATCGCAAGACCACTCGCCGCGGTTCATCCAAATACATGGAAAAGCAGTTACCATTCACAGGAATTTTGAGCAATAAACCTGAAGAAAACCCCGATTTCTTCAACTGGAACAGGGTCAAGCTACGATACTGTGATGGGGCATCTTTTGCTGGAGACAGCCAGAATGAGGCTGCACAACTTAATTTTCGAGGGCAAAAGATTTGGTTGGTTGCTATGGAAGAGTTGATGTCTAAGGGAATGCAGAATGCTAACCAGGCTCTTCTTTCTGGATGTTCTGCTGGGGGTCTGGCATCCATACTACACTGTGATGAGTTCCGGGACTTGTTTCCAACCAGTACCACAGTGAAATGTCTGAGTGATGCTGGGATGTTCCTTGATGCAATTGATGTGTCCGGTGGGCACACACTAAGGAATTTGTATGCAGGAGTAGTTAGCTTGCAGGAAGTGCAAAAGAACCTTCCAAAGACTTGTATCAACCACCTAGATCCAACTTCGTGCTTTTTTCCCCAGAATTTGGTCGCAAATGTTAAGACGCCAATGTTTCTTCTAAATGCAGCCTATGATGCTTGGCAGCTCCAAGCTAGTTTAGCCCCTCCATCTGCTGATCCTAATGGTTTATGGAATGAATGCAAATCAAACCATGCAAATTGTAATTTATCCCAGATCCAGTTTCTCCAAGATTTCAGGAATCAAATGCTCAGTGCTGTGAAAGATTTCTCAATGTCCCGTGAAAATGGTTTATTCATAAACTCTTGCTTTGCTCACTGCCAGTCTGAGAGACAAGATACATGGTTTGCTGATGATTCTCCTGTTCTTGAAAACCAGGCAATTGCACTGTCTGTTGGAGACTGGTACTTTGATCGAAGGACGGTTAAAGCTGTTGACTGTGAATACCCATGTGATAGCACCTGTCACAATCTTAACTTCAAAGAGGATGTAACTTCTACGACATTGACATATTCTCAATCTGCAAAGTTGTCTATTACTGTGCTAAATCTGCTAGGTGCCTTGTTGATGCCTATAACGTGTTCGAGATGCATAACGTGGTTTTCATTTGTCCACTGA

Protein Analysis

469

Amino Acids

51.57

Weight (kDa)

5.74

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 57 - 420 5.4e-174 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1349
AccB7I CCANNNNNTGG 1 cut(s) 859
AccII CGCG 1 cut(s) 366
AciI CCGC 3 cut(s) 245, 364, 366
AclI AACGTT 1 cut(s) 102
AclWI GGATC 6 cut(s) 270, 270, 283, 824, 944, 1003
AcsI RAATTY 3 cut(s) 408, 757, 856
AcuI CTGAAG 1 cut(s) 447
AcyI GRCGYC 1 cut(s) 878
AfaI GTAC 3 cut(s) 19, 682, 1199
AfeI AGCGCT 1 cut(s) 208
AfiI CCNNNNNNNGG 3 cut(s) 404, 511, 859
AflIII ACRYGT 1 cut(s) 1370
AgsI TTSAA 4 cut(s) 88, 448, 1163, 1273
AjnI CCWGG 2 cut(s) 597, 1167
AluBI AGCT 9 cut(s) 7, 44, 224, 291, 466, 780, 919, 926, 1223
AluI AGCT 9 cut(s) 7, 44, 224, 291, 466, 780, 919, 926, 1223
Alw26I GTCTC 3 cut(s) 492, 1117, 1185
AlwI GGATC 6 cut(s) 270, 270, 283, 824, 944, 1003
Aor51HI AGCGCT 1 cut(s) 208
AoxI GGCC 2 cut(s) 147, 186
ApeKI GCWGC 5 cut(s) 41, 209, 515, 899, 916
ApoI RAATTY 3 cut(s) 408, 757, 856
ArsI GACNNNNNNTTYG 2 cut(s) 1184, 1216
Asp700I GAANNNNTTC 1 cut(s) 1058
AspLEI GCGC 1 cut(s) 209
AspS9I GGNCC 1 cut(s) 187
AsuC2I CCSGG 2 cut(s) 249, 662
AsuHPI GGTGA 1 cut(s) 150
BaeGI GKGCMC 1 cut(s) 747
BamHI GGATCC 1 cut(s) 275
BanI GGYRCC 1 cut(s) 1349
BbvCI CCTCAGC 2 cut(s) 151, 171
BbvI GCAGC 5 cut(s) 53, 196, 502, 911, 928
BccI CCATC 4 cut(s) 230, 264, 473, 949
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 2 cut(s) 599, 1169
BcnI CCSGG 2 cut(s) 249, 662
BcoDI GTCTC 3 cut(s) 492, 1117, 1185
BfaI CTAG 3 cut(s) 827, 927, 1346
BfoI RGCGCY 1 cut(s) 210
BisI GCNGC 7 cut(s) 42, 210, 245, 364, 516, 900, 917
BlsI GCNGC 7 cut(s) 43, 211, 246, 365, 517, 901, 918
Bme1390I CCNGG 4 cut(s) 249, 599, 662, 1169
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 2 cut(s) 277, 1351
BmrFI CCNGG 4 cut(s) 249, 599, 662, 1169
BmsI GCATC 8 cut(s) 106, 494, 644, 694, 712, 898, 1350, 1370
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 2 cut(s) 151, 171
BpuMI CCSGG 2 cut(s) 249, 662
BsaHI GRCGYC 1 cut(s) 878
BsaJI CCNNGG 1 cut(s) 364
BsaWI WCCGGW 2 cut(s) 278, 737
Bsc4I CCNNNNNNNGG 3 cut(s) 404, 511, 859
Bse1I ACTGG 5 cut(s) 455, 678, 1012, 1114, 1199
BseBI CCWGG 2 cut(s) 599, 1169
BseDI CCNNGG 1 cut(s) 364
BseGI GGATG 6 cut(s) 241, 371, 620, 635, 717, 1285
BseLI CCNNNNNNNGG 3 cut(s) 404, 511, 859
BseMII CTCAG 5 cut(s) 165, 185, 689, 1057, 1110
BseNI ACTGG 5 cut(s) 455, 678, 1012, 1114, 1199
BseSI GKGCMC 1 cut(s) 747
BseXI GCAGC 5 cut(s) 53, 196, 502, 911, 928
BseYI CCCAGC 2 cut(s) 623, 707
BsgI GTGCAG 1 cut(s) 501
Bsh1236I CGCG 1 cut(s) 366
BshFI GGCC 2 cut(s) 149, 188
BshNI GGYRCC 1 cut(s) 1349
BsiSI CCGG 4 cut(s) 249, 279, 661, 738
BslFI GGGAC 2 cut(s) 677, 1054
BslI CCNNNNNNNGG 3 cut(s) 404, 511, 859
BsmAI GTCTC 3 cut(s) 492, 1117, 1185
BsmFI GGGAC 2 cut(s) 677, 1054
BsmI GAATGC 3 cut(s) 588, 595, 977
BsnI GGCC 2 cut(s) 149, 188
Bsp1286I GDGCHC 1 cut(s) 747
Bsp143I GATC 7 cut(s) 136, 262, 275, 829, 949, 1008, 1204
BspACI CCGC 3 cut(s) 245, 364, 366
BspANI GGCC 2 cut(s) 149, 188
BspCNI CTCAG 5 cut(s) 164, 184, 690, 1056, 1111
BspFNI CGCG 1 cut(s) 366
BspLI GGNNCC 2 cut(s) 277, 1351
BspPI GGATC 6 cut(s) 270, 270, 283, 824, 944, 1003
BspQI GCTCTTC 1 cut(s) 609
BspT107I GGYRCC 1 cut(s) 1349
BsrI ACTGG 5 cut(s) 455, 678, 1012, 1114, 1199
BssECI CCNNGG 1 cut(s) 364
BssMI GATC 7 cut(s) 136, 262, 275, 829, 949, 1008, 1204
BssNI GRCGYC 1 cut(s) 878
Bst2UI CCWGG 2 cut(s) 599, 1169
Bst4CI ACNGT 8 cut(s) 22, 476, 650, 688, 1182, 1216, 1232, 1333
Bst6I CTCTTC 2 cut(s) 558, 609
BstACI GRCGYC 1 cut(s) 878
BstAPI GCANNNNNTGC 2 cut(s) 491, 790
BstC8I GCNNGC 2 cut(s) 293, 782
BstDEI CTNAG 7 cut(s) 151, 171, 576, 698, 752, 1043, 1119
BstDSI CCRYGG 1 cut(s) 364
BstEII GGTNACC 1 cut(s) 251
BstF5I GGATG 6 cut(s) 241, 371, 620, 635, 717, 1285
BstFNI CGCG 1 cut(s) 366
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 1 cut(s) 209
BstKTI GATC 7 cut(s) 139, 265, 278, 832, 952, 1011, 1207
BstMAI GTCTC 3 cut(s) 492, 1117, 1185
BstMBI GATC 7 cut(s) 136, 262, 275, 829, 949, 1008, 1204
BstMWI GCNNNNNNNGC 7 cut(s) 41, 491, 599, 790, 908, 932, 1342
BstNI CCWGG 2 cut(s) 599, 1169
BstPI GGTNACC 1 cut(s) 251
BstSCI CCNGG 4 cut(s) 247, 597, 660, 1167
BstSLI GKGCMC 1 cut(s) 747
BstUI CGCG 1 cut(s) 366
BstV1I GCAGC 5 cut(s) 53, 196, 502, 911, 928
BstX2I RGATCY 3 cut(s) 275, 829, 1008
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 3 cut(s) 275, 829, 1008
BsuRI GGCC 2 cut(s) 149, 188
BtgI CCRYGG 1 cut(s) 364
BtsCI GGATG 6 cut(s) 241, 371, 620, 635, 717, 1285
BtsI GCAGTG 1 cut(s) 1108
BtsIMutI CAGTG 8 cut(s) 27, 102, 646, 693, 1051, 1108, 1178, 1405
Cac8I GCNNGC 2 cut(s) 293, 782
CfoI GCGC 1 cut(s) 209
Cfr13I GGNCC 1 cut(s) 187
Cfr42I CCGCGG 1 cut(s) 367
CseI GACGC 1 cut(s) 886
Csp6I GTAC 3 cut(s) 18, 681, 1198
CviAII CATG 6 cut(s) 140, 181, 382, 986, 1134, 1242
CviQI GTAC 3 cut(s) 18, 681, 1198
DdeI CTNAG 7 cut(s) 151, 171, 576, 698, 752, 1043, 1119
DpnI GATC 7 cut(s) 138, 264, 277, 831, 951, 1010, 1206
DpnII GATC 7 cut(s) 136, 262, 275, 829, 949, 1008, 1204
Eam1104I CTCTTC 2 cut(s) 558, 609
EarI CTCTTC 2 cut(s) 558, 609
Eco47III AGCGCT 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 447
Eco91I GGTNACC 1 cut(s) 251
EcoO65I GGTNACC 1 cut(s) 251
EcoRII CCWGG 2 cut(s) 597, 1167
EcoT22I ATGCAT 1 cut(s) 1385
FaeI CATG 6 cut(s) 143, 184, 385, 989, 1137, 1245
FaqI GGGAC 2 cut(s) 677, 1054
FatI CATG 6 cut(s) 139, 180, 381, 985, 1133, 1241
Fnu4HI GCNGC 7 cut(s) 42, 210, 245, 364, 516, 900, 917
FokI GGATG 6 cut(s) 248, 358, 622, 627, 724, 1292
Fsp4HI GCNGC 7 cut(s) 42, 210, 245, 364, 516, 900, 917
FspBI CTAG 3 cut(s) 827, 927, 1346
GlaI GCGC 1 cut(s) 208
GluI GCNGC 7 cut(s) 42, 210, 245, 364, 516, 900, 917
GsaI CCCAGC 2 cut(s) 627, 711
GsuI CTGGAG 1 cut(s) 516
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 2 cut(s) 149, 188
HapII CCGG 4 cut(s) 249, 279, 661, 738
HgaI GACGC 1 cut(s) 886
HhaI GCGC 1 cut(s) 209
Hin1I GRCGYC 1 cut(s) 878
Hin1II CATG 6 cut(s) 143, 184, 385, 989, 1137, 1245
Hin6I GCGC 1 cut(s) 207
HinP1I GCGC 1 cut(s) 207
HincII GTYRAC 1 cut(s) 1228
HindII GTYRAC 1 cut(s) 1228
HinfI GANTC 3 cut(s) 92, 1033, 1148
HpaII CCGG 4 cut(s) 249, 279, 661, 738
HphI GGTGA 1 cut(s) 150
Hpy166II GTNNAC 3 cut(s) 100, 1228, 1405
Hpy188I TCNGA 4 cut(s) 60, 335, 699, 1120
Hpy188III TCNNGA 6 cut(s) 260, 273, 612, 1030, 1160, 1377
Hpy8I GTNNAC 3 cut(s) 100, 1228, 1405
HpyAV CCTTC 2 cut(s) 812, 1203
HpyCH4III ACNGT 8 cut(s) 22, 476, 650, 688, 1182, 1216, 1232, 1333
HpyCH4IV ACGT 3 cut(s) 102, 1370, 1388
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 491, 599, 790, 908, 932, 1342
HpyF3I CTNAG 7 cut(s) 151, 171, 576, 698, 752, 1043, 1119
HpySE526I ACGT 3 cut(s) 102, 1370, 1388
Hsp92I GRCGYC 1 cut(s) 878
Hsp92II CATG 6 cut(s) 143, 184, 385, 989, 1137, 1245
HspAI GCGC 1 cut(s) 207
KspI CCGCGG 1 cut(s) 367
Kzo9I GATC 7 cut(s) 136, 262, 275, 829, 949, 1008, 1204
LguI GCTCTTC 1 cut(s) 609
LmnI GCTCC 2 cut(s) 221, 924
Lsp1109I GCAGC 5 cut(s) 53, 196, 502, 911, 928
LweI GCATC 8 cut(s) 106, 494, 644, 694, 712, 898, 1350, 1370
MaeI CTAG 3 cut(s) 827, 927, 1346
MaeII ACGT 3 cut(s) 102, 1370, 1388
MaeIII GTNAC 4 cut(s) 251, 393, 1256, 1282
MalI GATC 7 cut(s) 138, 264, 277, 831, 951, 1010, 1206
MboI GATC 7 cut(s) 136, 262, 275, 829, 949, 1008, 1204
MboII GAAGA 5 cut(s) 436, 440, 575, 596, 881
MfeI CAATTG 2 cut(s) 726, 1173
MflI RGATCY 3 cut(s) 275, 829, 1008
MhlI GDGCHC 1 cut(s) 747
MluCI AATT 9 cut(s) 337, 408, 526, 726, 757, 856, 991, 997, 1173
MmeI TCCRAC 4 cut(s) 285, 698, 857, 1168
Mph1103I ATGCAT 1 cut(s) 1385
MroXI GAANNNNTTC 1 cut(s) 1058
MseI TTAA 4 cut(s) 525, 873, 1218, 1266
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 3 cut(s) 44, 291, 366
MspI CCGG 4 cut(s) 249, 279, 661, 738
MspR9I CCNGG 4 cut(s) 249, 599, 662, 1169
MunI CAATTG 2 cut(s) 726, 1173
Mva1269I GAATGC 3 cut(s) 588, 595, 977
MvaI CCWGG 2 cut(s) 599, 1169
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 7 cut(s) 41, 491, 599, 790, 908, 932, 1342
NciI CCSGG 2 cut(s) 249, 662
NdeII GATC 7 cut(s) 136, 262, 275, 829, 949, 1008, 1204
NlaIII CATG 6 cut(s) 143, 184, 385, 989, 1137, 1245
NlaIV GGNNCC 2 cut(s) 277, 1351
NmuCI GTSAC 1 cut(s) 1256
NsiI ATGCAT 1 cut(s) 1385
PciSI GCTCTTC 1 cut(s) 609
PctI GAATGC 3 cut(s) 588, 595, 977
PdmI GAANNNNTTC 1 cut(s) 1058
PfeI GAWTC 3 cut(s) 92, 1033, 1148
PflMI CCANNNNNTGG 1 cut(s) 859
PfoI TCCNGGA 1 cut(s) 660
PkrI GCNGC 7 cut(s) 43, 211, 246, 365, 517, 901, 918
Psp1406I AACGTT 1 cut(s) 102
Psp6I CCWGG 2 cut(s) 597, 1167
PspEI GGTNACC 1 cut(s) 251
PspFI CCCAGC 2 cut(s) 623, 707
PspGI CCWGG 2 cut(s) 597, 1167
PspN4I GGNNCC 2 cut(s) 277, 1351
PspPI GGNCC 1 cut(s) 187
PsuI RGATCY 3 cut(s) 275, 829, 1008
PvuII CAGCTG 2 cut(s) 44, 291
RsaI GTAC 3 cut(s) 19, 682, 1199
RsaNI GTAC 3 cut(s) 18, 681, 1198
RseI CAYNNNNRTG 1 cut(s) 651
SacII CCGCGG 1 cut(s) 367
SapI GCTCTTC 1 cut(s) 609
SaqAI TTAA 4 cut(s) 525, 873, 1218, 1266
SatI GCNGC 7 cut(s) 42, 210, 245, 364, 516, 900, 917
Sau3AI GATC 7 cut(s) 136, 262, 275, 829, 949, 1008, 1204
Sau96I GGNCC 1 cut(s) 187
ScrFI CCNGG 4 cut(s) 249, 599, 662, 1169
SduI GDGCHC 1 cut(s) 747
SfaNI GCATC 8 cut(s) 106, 494, 644, 694, 712, 898, 1350, 1370
Sfr303I CCGCGG 1 cut(s) 367
SgrBI CCGCGG 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 651
Sse9I AATT 9 cut(s) 337, 408, 526, 726, 757, 856, 991, 997, 1173
SsiI CCGC 3 cut(s) 245, 364, 366
SspMI CTAG 3 cut(s) 827, 927, 1346
StyD4I CCNGG 4 cut(s) 247, 597, 660, 1167
TaaI ACNGT 8 cut(s) 22, 476, 650, 688, 1182, 1216, 1232, 1333
TaiI ACGT 3 cut(s) 105, 1373, 1391
TaqI TCGA 5 cut(s) 129, 201, 532, 1207, 1376
TasI AATT 9 cut(s) 337, 408, 526, 726, 757, 856, 991, 997, 1173
TauI GCSGC 2 cut(s) 247, 366
TfiI GAWTC 3 cut(s) 92, 1033, 1148
Tru1I TTAA 4 cut(s) 525, 873, 1218, 1266
Tru9I TTAA 4 cut(s) 525, 873, 1218, 1266
TscAI CASTG 7 cut(s) 27, 102, 653, 693, 1051, 1115, 1185
TseFI GTSAC 1 cut(s) 1256
TseI GCWGC 5 cut(s) 41, 209, 515, 899, 916
Tsp45I GTSAC 1 cut(s) 1256
TspDTI ATGAA 5 cut(s) 17, 360, 984, 1078, 1386
TspRI CASTG 7 cut(s) 27, 102, 653, 693, 1051, 1115, 1185
Van91I CCANNNNNTGG 1 cut(s) 859
XapI RAATTY 3 cut(s) 408, 757, 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XmnI GAANNNNTTC 1 cut(s) 1058
XspI CTAG 3 cut(s) 827, 927, 1346
Zsp2I ATGCAT 1 cut(s) 1385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.