Rh3BG304500

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002705
Physical Location & Seq
Forward (+)
30759086 .. 30763196
4111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG304500.1

Sequence Viewer

Length: 1293 bp
ATGAAGAAGCCGCAGCTGAAGCTGAATGTGGGTACAGTGTTGGTGGTGGCATTGGCAGCTGCCTTTGTCAATCTGAAACTCAATGTGGGTGCTTTGAGTTTGAACGAATCAGTGAACATTGAGAGGAAAGATGCAGTCTTGTTCGACAACGATCATGTCTCAGTCTCAGCCTCACCGACACAGCCTCAGCCTCTCATGGTGGCCCTCACTCTTATCGACAGCGCTGCTTCTAAAGGAGCTGTCTGTTTGGATGGAACACCGCCCGGTTACCATCTGGATCGTGGGTCTGGATCCGGTGCAAACAGCTGGCTCATTCAGTTGGAGGGAGGTGGGTGGTGTAATACTATCAGAAATTGTGTTTATCGCAAGACCACTCGCCGCGGTTCATCCAAATACATGGAAAAGCAATTACCATTCACAGGAATTTTGAGCAATAAACCTGAAGAAAACCCCGATTTCTTCAACTGGAACAGGGTCAAGCTACGATACTGTGATGGGGCATCTTTTGCCGGAGACAGCCAGAATGAGGCTGCACAACTTAATTTTCGAGGGCAAAAGATTTGGTTGGTTGCTATGGAAGAGTTGATGTCTAAGGGAATGCAGAATGCTAACCAGGCTCTTCTTTCTGGATGTTCCGCTGGGGGTCTGGCATCCATACTACACTGTGATGAGTTCCGGGACTTGTTTCCAACCAGTACCACAGTGAAATGTCTGAGTGATGCTGGGATGTTCCTTGATGCAATTGATGTATCTGGTGGGCACACACTAAGGAATTTGTATGCCGGAGTAGTTAGCTTGCAGGAAGTGCAAAAGAATCTTCCAAAGACTTGTATCAACCACCTAGATCCAACTTCGTGCTTTTTTCCTCAGAATTTGGTCGCAAATGTTAAGACGCCAATGTTTCTTCTAAATGCAGCCTATGATGCTTGGCAGCTCCAAGCTAGTTTAGCCCCTTCATCTGCTGATCCTAATGGTTTATGGAATGAATGCAAATCAAACCATGCAAATTGTAATTTATCCCAGATCCAGTTTCTCCAAGATTTTAGGAATCAAATGCTCAGTGCTGTGAAAGATTTCTCAATGTCCCGTGAAAATGGTTTATTCATAAACTCTTGCTTTGCTCACTGCCAGTCTGAGAGACAAGATACATGGTTTGCTGATGATTCTCCTGTTCTTGAAAACCAGGCAATTGCACTGTCTGTTGGAGACTGGTACTTTGATCGAAGGACGGTTAAAGCTATTGACTGTGAATACCCCTGTGATAGCACCTGTCACAATCTTAACTTCAAGTGA

Protein Analysis

430

Amino Acids

47.31

Weight (kDa)

5.93

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 62 - 425 1.8e-174 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 381
AciI CCGC 5 cut(s) 11, 260, 379, 381, 636
AclWI GGATC 6 cut(s) 285, 285, 298, 839, 959, 1018
AcsI RAATTY 3 cut(s) 423, 772, 871
AcuI CTGAAG 2 cut(s) 38, 462
AcyI GRCGYC 1 cut(s) 893
AfaI GTAC 3 cut(s) 34, 697, 1214
AfeI AGCGCT 1 cut(s) 223
AfiI CCNNNNNNNGG 2 cut(s) 419, 526
AgsI TTSAA 4 cut(s) 103, 463, 1178, 1288
AjnI CCWGG 2 cut(s) 612, 1182
Alw26I GTCTC 5 cut(s) 163, 169, 507, 1132, 1200
AlwI GGATC 6 cut(s) 285, 285, 298, 839, 959, 1018
Aor51HI AGCGCT 1 cut(s) 223
AoxI GGCC 1 cut(s) 201
ApeKI GCWGC 7 cut(s) 13, 56, 59, 224, 530, 914, 931
ApoI RAATTY 3 cut(s) 423, 772, 871
ArsI GACNNNNNNTTYG 2 cut(s) 1199, 1231
Asp700I GAANNNNTTC 1 cut(s) 1073
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 1 cut(s) 202
AsuC2I CCSGG 2 cut(s) 264, 677
AsuHPI GGTGA 1 cut(s) 165
BaeGI GKGCMC 1 cut(s) 762
BamHI GGATCC 1 cut(s) 290
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 7 cut(s) 25, 46, 68, 211, 517, 926, 943
BccI CCATC 3 cut(s) 245, 279, 488
BcgI CGANNNNNNTGC 2 cut(s) 206, 240
BciT130I CCWGG 2 cut(s) 614, 1184
BcnI CCSGG 2 cut(s) 264, 677
BcoDI GTCTC 5 cut(s) 163, 169, 507, 1132, 1200
BfaI CTAG 2 cut(s) 842, 942
BfoI RGCGCY 1 cut(s) 225
BisI GCNGC 9 cut(s) 11, 14, 57, 60, 225, 379, 531, 915, 932
BlsI GCNGC 9 cut(s) 12, 15, 58, 61, 226, 380, 532, 916, 933
Bme1390I CCNGG 4 cut(s) 264, 614, 677, 1184
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 1 cut(s) 292
BmrFI CCNGG 4 cut(s) 264, 614, 677, 1184
BmsI GCATC 6 cut(s) 121, 509, 659, 709, 727, 913
Bpu10I CCTNAGC 1 cut(s) 186
BpuMI CCSGG 2 cut(s) 264, 677
BsaHI GRCGYC 1 cut(s) 893
BsaJI CCNNGG 1 cut(s) 379
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 526
Bse1I ACTGG 5 cut(s) 470, 693, 1027, 1129, 1214
BseBI CCWGG 2 cut(s) 614, 1184
BseDI CCNNGG 1 cut(s) 379
BseGI GGATG 5 cut(s) 256, 386, 635, 650, 732
BseLI CCNNNNNNNGG 2 cut(s) 419, 526
BseMII CTCAG 7 cut(s) 174, 180, 200, 704, 881, 1072, 1125
BseNI ACTGG 5 cut(s) 470, 693, 1027, 1129, 1214
BseSI GKGCMC 1 cut(s) 762
BseXI GCAGC 7 cut(s) 25, 46, 68, 211, 517, 926, 943
BseYI CCCAGC 2 cut(s) 638, 722
BsgI GTGCAG 1 cut(s) 516
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 1 cut(s) 203
BsiSI CCGG 5 cut(s) 264, 294, 510, 676, 783
BslFI GGGAC 2 cut(s) 692, 1069
BslI CCNNNNNNNGG 2 cut(s) 419, 526
BsmAI GTCTC 5 cut(s) 163, 169, 507, 1132, 1200
BsmFI GGGAC 2 cut(s) 692, 1069
BsmI GAATGC 3 cut(s) 603, 610, 992
BsnI GGCC 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 762
Bsp143I GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
BspACI CCGC 5 cut(s) 11, 260, 379, 381, 636
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 7 cut(s) 173, 179, 199, 705, 880, 1071, 1126
BspFNI CGCG 1 cut(s) 381
BspLI GGNNCC 1 cut(s) 292
BspPI GGATC 6 cut(s) 285, 285, 298, 839, 959, 1018
BspQI GCTCTTC 1 cut(s) 624
BsrI ACTGG 5 cut(s) 470, 693, 1027, 1129, 1214
BssECI CCNNGG 1 cut(s) 379
BssMI GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
BssNI GRCGYC 1 cut(s) 893
Bst2UI CCWGG 2 cut(s) 614, 1184
Bst4CI ACNGT 7 cut(s) 37, 491, 665, 703, 1197, 1231, 1247
Bst6I CTCTTC 2 cut(s) 573, 624
BstACI GRCGYC 1 cut(s) 893
BstAPI GCANNNNNTGC 2 cut(s) 506, 805
BstC8I GCNNGC 2 cut(s) 308, 797
BstDEI CTNAG 9 cut(s) 160, 166, 186, 591, 713, 767, 867, 1058, 1134
BstDSI CCRYGG 1 cut(s) 379
BstEII GGTNACC 1 cut(s) 266
BstF5I GGATG 5 cut(s) 256, 386, 635, 650, 732
BstFNI CGCG 1 cut(s) 381
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 7 cut(s) 154, 280, 293, 847, 967, 1026, 1222
BstMAI GTCTC 5 cut(s) 163, 169, 507, 1132, 1200
BstMBI GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
BstMWI GCNNNNNNNGC 7 cut(s) 19, 56, 506, 614, 805, 923, 947
BstNI CCWGG 2 cut(s) 614, 1184
BstPI GGTNACC 1 cut(s) 266
BstSCI CCNGG 4 cut(s) 262, 612, 675, 1182
BstSLI GKGCMC 1 cut(s) 762
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 7 cut(s) 25, 46, 68, 211, 517, 926, 943
BstX2I RGATCY 3 cut(s) 290, 844, 1023
BstXI CCANNNNNNTGG 1 cut(s) 397
BstYI RGATCY 3 cut(s) 290, 844, 1023
BsuRI GGCC 1 cut(s) 203
BtgI CCRYGG 1 cut(s) 379
BtsCI GGATG 5 cut(s) 256, 386, 635, 650, 732
BtsI GCAGTG 1 cut(s) 1123
BtsIMutI CAGTG 7 cut(s) 42, 117, 661, 708, 1066, 1123, 1193
Cac8I GCNNGC 2 cut(s) 308, 797
CfoI GCGC 1 cut(s) 224
Cfr13I GGNCC 1 cut(s) 202
Cfr42I CCGCGG 1 cut(s) 382
CseI GACGC 1 cut(s) 901
Csp6I GTAC 3 cut(s) 33, 696, 1213
CviAII CATG 5 cut(s) 155, 196, 397, 1001, 1149
CviQI GTAC 3 cut(s) 33, 696, 1213
DdeI CTNAG 9 cut(s) 160, 166, 186, 591, 713, 767, 867, 1058, 1134
DpnI GATC 7 cut(s) 153, 279, 292, 846, 966, 1025, 1221
DpnII GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
Eam1104I CTCTTC 2 cut(s) 573, 624
EarI CTCTTC 2 cut(s) 573, 624
Eco47III AGCGCT 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 38, 462
Eco91I GGTNACC 1 cut(s) 266
EcoO65I GGTNACC 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 612, 1182
FaeI CATG 5 cut(s) 158, 199, 400, 1004, 1152
FaqI GGGAC 2 cut(s) 692, 1069
FatI CATG 5 cut(s) 154, 195, 396, 1000, 1148
Fnu4HI GCNGC 9 cut(s) 11, 14, 57, 60, 225, 379, 531, 915, 932
FokI GGATG 5 cut(s) 263, 373, 637, 642, 739
Fsp4HI GCNGC 9 cut(s) 11, 14, 57, 60, 225, 379, 531, 915, 932
FspBI CTAG 2 cut(s) 842, 942
GlaI GCGC 1 cut(s) 223
GluI GCNGC 9 cut(s) 11, 14, 57, 60, 225, 379, 531, 915, 932
GsaI CCCAGC 2 cut(s) 642, 726
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 1 cut(s) 203
HapII CCGG 5 cut(s) 264, 294, 510, 676, 783
HgaI GACGC 1 cut(s) 901
HhaI GCGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 893
Hin1II CATG 5 cut(s) 158, 199, 400, 1004, 1152
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HinfI GANTC 4 cut(s) 107, 814, 1048, 1163
HpaII CCGG 5 cut(s) 264, 294, 510, 676, 783
HphI GGTGA 1 cut(s) 165
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 5 cut(s) 75, 350, 714, 870, 1135
Hpy188III TCNNGA 4 cut(s) 275, 288, 627, 1175
Hpy8I GTNNAC 1 cut(s) 115
HpyAV CCTTC 2 cut(s) 963, 1218
HpyCH4III ACNGT 7 cut(s) 37, 491, 665, 703, 1197, 1231, 1247
HpyF10VI GCNNNNNNNGC 7 cut(s) 19, 56, 506, 614, 805, 923, 947
HpyF3I CTNAG 9 cut(s) 160, 166, 186, 591, 713, 767, 867, 1058, 1134
Hsp92I GRCGYC 1 cut(s) 893
Hsp92II CATG 5 cut(s) 158, 199, 400, 1004, 1152
HspAI GCGC 1 cut(s) 222
KspI CCGCGG 1 cut(s) 382
Kzo9I GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
LguI GCTCTTC 1 cut(s) 624
LmnI GCTCC 2 cut(s) 236, 939
Lsp1109I GCAGC 7 cut(s) 25, 46, 68, 211, 517, 926, 943
LweI GCATC 6 cut(s) 121, 509, 659, 709, 727, 913
MaeI CTAG 2 cut(s) 842, 942
MaeIII GTNAC 2 cut(s) 266, 1271
MalI GATC 7 cut(s) 153, 279, 292, 846, 966, 1025, 1221
MboI GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
MboII GAAGA 7 cut(s) 16, 451, 455, 590, 611, 809, 896
MfeI CAATTG 2 cut(s) 741, 1188
MflI RGATCY 3 cut(s) 290, 844, 1023
MhlI GDGCHC 1 cut(s) 762
MmeI TCCRAC 4 cut(s) 300, 713, 872, 1183
MroXI GAANNNNTTC 1 cut(s) 1073
MseI TTAA 4 cut(s) 540, 888, 1233, 1281
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 5 cut(s) 16, 59, 306, 381, 638
MspI CCGG 5 cut(s) 264, 294, 510, 676, 783
MspR9I CCNGG 4 cut(s) 264, 614, 677, 1184
MunI CAATTG 2 cut(s) 741, 1188
Mva1269I GAATGC 3 cut(s) 603, 610, 992
MvaI CCWGG 2 cut(s) 614, 1184
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 7 cut(s) 19, 56, 506, 614, 805, 923, 947
NciI CCSGG 2 cut(s) 264, 677
NdeII GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
NlaIII CATG 5 cut(s) 158, 199, 400, 1004, 1152
NlaIV GGNNCC 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 1271
PciSI GCTCTTC 1 cut(s) 624
PctI GAATGC 3 cut(s) 603, 610, 992
PdmI GAANNNNTTC 1 cut(s) 1073
PfeI GAWTC 4 cut(s) 107, 814, 1048, 1163
PfoI TCCNGGA 1 cut(s) 675
PkrI GCNGC 9 cut(s) 12, 15, 58, 61, 226, 380, 532, 916, 933
Psp6I CCWGG 2 cut(s) 612, 1182
PspEI GGTNACC 1 cut(s) 266
PspFI CCCAGC 2 cut(s) 638, 722
PspGI CCWGG 2 cut(s) 612, 1182
PspN4I GGNNCC 1 cut(s) 292
PspPI GGNCC 1 cut(s) 202
PsuI RGATCY 3 cut(s) 290, 844, 1023
PvuII CAGCTG 3 cut(s) 16, 59, 306
RsaI GTAC 3 cut(s) 34, 697, 1214
RsaNI GTAC 3 cut(s) 33, 696, 1213
RseI CAYNNNNRTG 1 cut(s) 666
SacII CCGCGG 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 624
SaqAI TTAA 4 cut(s) 540, 888, 1233, 1281
SatI GCNGC 9 cut(s) 11, 14, 57, 60, 225, 379, 531, 915, 932
Sau3AI GATC 7 cut(s) 151, 277, 290, 844, 964, 1023, 1219
Sau96I GGNCC 1 cut(s) 202
ScrFI CCNGG 4 cut(s) 264, 614, 677, 1184
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 6 cut(s) 121, 509, 659, 709, 727, 913
Sfr303I CCGCGG 1 cut(s) 382
SgrBI CCGCGG 1 cut(s) 382
SmiMI CAYNNNNRTG 1 cut(s) 666
SsiI CCGC 5 cut(s) 11, 260, 379, 381, 636
SspMI CTAG 2 cut(s) 842, 942
StyD4I CCNGG 4 cut(s) 262, 612, 675, 1182
TaaI ACNGT 7 cut(s) 37, 491, 665, 703, 1197, 1231, 1247
TaqI TCGA 4 cut(s) 144, 216, 547, 1222
TauI GCSGC 2 cut(s) 13, 381
TfiI GAWTC 4 cut(s) 107, 814, 1048, 1163
Tru1I TTAA 4 cut(s) 540, 888, 1233, 1281
Tru9I TTAA 4 cut(s) 540, 888, 1233, 1281
TscAI CASTG 7 cut(s) 42, 117, 668, 708, 1066, 1130, 1200
TseFI GTSAC 1 cut(s) 1271
TseI GCWGC 7 cut(s) 13, 56, 59, 224, 530, 914, 931
Tsp45I GTSAC 1 cut(s) 1271
TspDTI ATGAA 5 cut(s) 17, 375, 945, 999, 1093
TspRI CASTG 7 cut(s) 42, 117, 668, 708, 1066, 1130, 1200
XapI RAATTY 3 cut(s) 423, 772, 871
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 1073
XspI CTAG 2 cut(s) 842, 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.