RchiOBHm_Chr3g0485621

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002705
Physical Location & Seq
Forward (+)
32359626 .. 32365095
5470 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45021

Sequence Viewer

Length: 1416 bp
ATGAAGAAGCAGCAGCAGCTGAATGTGGGTACAGTGTTGGTGGTGGCATTGGCAGCTGCCTTTGTCAATCTGAAACTCAATGTGGGTGCTTTAAGTTTGAACGAATCAGTGAACGTTGAGAGAAAAGATGCAGTCTTTTTCGACAACGATCATGTCTCAGCCTCACCGACACAGCCTCAGCCTCTCATGGTGGCCCTCACTCTTATCGACAGCGCTGCTTCTAAAGGAGCTGTCTGTTTGGATGGAACACCGCCCGGTTACCATCTGGATCGTGGGTCTGGATCCGGTGCAAACAGCTGGCTCATTCAGTTGGAGGGAGGTGGGTGGTGTAATACTATCAGAAATTGTGTTTATCGCAAGACCACTCGCCGCGGTTCATCCAAATACATGGAAAAGCAATTACCATTCACAGGAATTTTGAGCAATAAACCTGAAGAAAACCCCGATTTCTTCAACTGGAACAGGGTCAAGCTACGATACTGTGATGGGGCATCTTTTGCCGGAGACAGCCAGAATGAGGCTGCACAACTTAATTTTCGAGGGCAAAAGATTTGGTTGGTTGCTATGGAAGAATTGATGTCTAAGGGAATGCAGAATGCTAACCAGGCTCTTCTTTCTGGATGTTCCGCTGGGGGTCTGGCATCCATACTACACTGTGATGAGTTCCGGGACTTGTTTCCAACCAGTACCACAGTGAAATGTCTGAGTGATGCTGGGATGTTCCTTGATGCAATTGATGTATCTGGTGGGCACACACTAAGGAATTTGTATGCAGGAGTAGTTAGCTTGCAGGAAGTGCAAAAGAATCTTCCAAAGACTTGTATCAACCACCTAGATCCAACTTCGTGCTTTTTTCCTCAGAATTTGGTCGCAAATGTTAAGACGCCAATGTTTCTTCTAAATGCAGCCTATGATGCTTGGCAGCTCCAAGCTAGTTTAGCCCCTCCATCTGCTGATCCTAATGGTTTATGGAATGAATGCAAATCAAACCATGCAAATTGTAATTTATCCCAGATCCAGTTTCTCCAAGATTTCAGGAATCAAATGCTCAGTGCTGTGAAAGATTTCTCAATGTCCCGTGAAAATGGTTTATTCATAAACTCTTGCTTTGCTCACTGCCAGTCTGAGAGACAAGATACATGGTTTGCTGATGATTCTCCTGTTCTTGAAAGCCAGGCAATTGCACTGTCTGTGGGAGACTGGTACTTTGATCGAAGGACAGTTAAAGCTATTGACTGTGAATACCCCTGTGATAGCACCTGTCACAATCTTAACTTCAAAGAGGATGTAACTTCTACGACATTGACATATTCTCAGTCTGCAAAGTCGTCTATTACCGTGCTAAATCTGCTAGGTGCCTTGTTGATGCCTATAATGTGTTCGAGATGCATAACGTGGTTTTCATTTGTCCACTGA

Protein Analysis

471

Amino Acids

51.89

Weight (kDa)

5.87

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 59 - 422 4.1e-174 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1355
AccII CGCG 1 cut(s) 372
AciI CCGC 4 cut(s) 251, 370, 372, 627
AclI AACGTT 1 cut(s) 114
AclWI GGATC 6 cut(s) 276, 276, 289, 830, 950, 1009
AcsI RAATTY 3 cut(s) 414, 763, 862
AcuI CTGAAG 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 884
AfaI GTAC 3 cut(s) 31, 688, 1205
AfeI AGCGCT 1 cut(s) 214
AfiI CCNNNNNNNGG 2 cut(s) 410, 517
AgsI TTSAA 4 cut(s) 100, 454, 1169, 1279
AjnI CCWGG 2 cut(s) 603, 1173
AluBI AGCT 9 cut(s) 19, 56, 230, 297, 472, 786, 925, 932, 1229
AluI AGCT 9 cut(s) 19, 56, 230, 297, 472, 786, 925, 932, 1229
Alw26I GTCTC 4 cut(s) 160, 498, 1123, 1191
AlwI GGATC 6 cut(s) 276, 276, 289, 830, 950, 1009
AlwNI CAGNNNCTG 1 cut(s) 19
Aor51HI AGCGCT 1 cut(s) 214
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 9 cut(s) 10, 13, 16, 53, 56, 215, 521, 905, 922
ApoI RAATTY 3 cut(s) 414, 763, 862
ArsI GACNNNNNNTTYG 2 cut(s) 1190, 1222
Asp700I GAANNNNTTC 1 cut(s) 1064
AspLEI GCGC 1 cut(s) 215
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 2 cut(s) 255, 668
AsuHPI GGTGA 1 cut(s) 156
BaeGI GKGCMC 1 cut(s) 753
BamHI GGATCC 1 cut(s) 281
BanI GGYRCC 1 cut(s) 1355
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 9 cut(s) 22, 25, 28, 43, 65, 202, 508, 917, 934
BccI CCATC 4 cut(s) 236, 270, 479, 955
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 2 cut(s) 605, 1175
BcnI CCSGG 2 cut(s) 255, 668
BcoDI GTCTC 4 cut(s) 160, 498, 1123, 1191
BfaI CTAG 3 cut(s) 833, 933, 1352
BfoI RGCGCY 1 cut(s) 216
Bme1390I CCNGG 4 cut(s) 255, 605, 668, 1175
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 2 cut(s) 283, 1357
BmrFI CCNGG 4 cut(s) 255, 605, 668, 1175
BmsI GCATC 8 cut(s) 118, 500, 650, 700, 718, 904, 1356, 1376
Bpu10I CCTNAGC 1 cut(s) 177
BpuMI CCSGG 2 cut(s) 255, 668
BsaHI GRCGYC 1 cut(s) 884
BsaJI CCNNGG 1 cut(s) 370
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 2 cut(s) 410, 517
Bse1I ACTGG 5 cut(s) 461, 684, 1018, 1120, 1205
BseBI CCWGG 2 cut(s) 605, 1175
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 6 cut(s) 247, 377, 626, 641, 723, 1291
BseLI CCNNNNNNNGG 2 cut(s) 410, 517
BseMII CTCAG 7 cut(s) 171, 191, 695, 872, 1063, 1116, 1328
BseNI ACTGG 5 cut(s) 461, 684, 1018, 1120, 1205
BseSI GKGCMC 1 cut(s) 753
BseXI GCAGC 9 cut(s) 22, 25, 28, 43, 65, 202, 508, 917, 934
BseYI CCCAGC 2 cut(s) 629, 713
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 1 cut(s) 372
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 1355
BsiSI CCGG 4 cut(s) 255, 285, 501, 667
BslFI GGGAC 2 cut(s) 683, 1060
BslI CCNNNNNNNGG 2 cut(s) 410, 517
BsmAI GTCTC 4 cut(s) 160, 498, 1123, 1191
BsmFI GGGAC 2 cut(s) 683, 1060
BsmI GAATGC 3 cut(s) 594, 601, 983
BsnI GGCC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 753
Bsp143I GATC 7 cut(s) 148, 268, 281, 835, 955, 1014, 1210
BspACI CCGC 4 cut(s) 251, 370, 372, 627
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 7 cut(s) 170, 190, 696, 871, 1062, 1117, 1327
BspFNI CGCG 1 cut(s) 372
BspLI GGNNCC 2 cut(s) 283, 1357
BspPI GGATC 6 cut(s) 276, 276, 289, 830, 950, 1009
BspQI GCTCTTC 1 cut(s) 615
BspT107I GGYRCC 1 cut(s) 1355
BsrI ACTGG 5 cut(s) 461, 684, 1018, 1120, 1205
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 7 cut(s) 148, 268, 281, 835, 955, 1014, 1210
BssNI GRCGYC 1 cut(s) 884
Bst2UI CCWGG 2 cut(s) 605, 1175
Bst4CI ACNGT 8 cut(s) 34, 482, 656, 694, 1188, 1222, 1238, 1339
Bst6I CTCTTC 1 cut(s) 615
BstACI GRCGYC 1 cut(s) 884
BstAPI GCANNNNNTGC 2 cut(s) 497, 796
BstC8I GCNNGC 2 cut(s) 299, 788
BstDEI CTNAG 9 cut(s) 157, 177, 582, 704, 758, 858, 1049, 1125, 1314
BstDSI CCRYGG 1 cut(s) 370
BstEII GGTNACC 1 cut(s) 257
BstF5I GGATG 6 cut(s) 247, 377, 626, 641, 723, 1291
BstFNI CGCG 1 cut(s) 372
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 7 cut(s) 151, 271, 284, 838, 958, 1017, 1213
BstMAI GTCTC 4 cut(s) 160, 498, 1123, 1191
BstMBI GATC 7 cut(s) 148, 268, 281, 835, 955, 1014, 1210
BstMWI GCNNNNNNNGC 8 cut(s) 16, 53, 497, 605, 796, 914, 938, 1348
BstNI CCWGG 2 cut(s) 605, 1175
BstPI GGTNACC 1 cut(s) 257
BstSCI CCNGG 4 cut(s) 253, 603, 666, 1173
BstSLI GKGCMC 1 cut(s) 753
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 9 cut(s) 22, 25, 28, 43, 65, 202, 508, 917, 934
BstX2I RGATCY 3 cut(s) 281, 835, 1014
BstXI CCANNNNNNTGG 1 cut(s) 388
BstYI RGATCY 3 cut(s) 281, 835, 1014
BsuRI GGCC 1 cut(s) 194
BtgI CCRYGG 1 cut(s) 370
BtsCI GGATG 6 cut(s) 247, 377, 626, 641, 723, 1291
BtsI GCAGTG 1 cut(s) 1114
BtsIMutI CAGTG 8 cut(s) 39, 114, 652, 699, 1057, 1114, 1184, 1411
Cac8I GCNNGC 2 cut(s) 299, 788
CaiI CAGNNNCTG 1 cut(s) 19
CfoI GCGC 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 193
Cfr42I CCGCGG 1 cut(s) 373
CseI GACGC 1 cut(s) 892
Csp6I GTAC 3 cut(s) 30, 687, 1204
CviAII CATG 5 cut(s) 152, 187, 388, 992, 1140
CviQI GTAC 3 cut(s) 30, 687, 1204
DdeI CTNAG 9 cut(s) 157, 177, 582, 704, 758, 858, 1049, 1125, 1314
DpnI GATC 7 cut(s) 150, 270, 283, 837, 957, 1016, 1212
DpnII GATC 7 cut(s) 148, 268, 281, 835, 955, 1014, 1210
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
Eco47III AGCGCT 1 cut(s) 214
Eco57I CTGAAG 1 cut(s) 453
Eco91I GGTNACC 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 603, 1173
EcoT22I ATGCAT 1 cut(s) 1391
FaeI CATG 5 cut(s) 155, 190, 391, 995, 1143
FaqI GGGAC 2 cut(s) 683, 1060
FatI CATG 5 cut(s) 151, 186, 387, 991, 1139
FokI GGATG 6 cut(s) 254, 364, 628, 633, 730, 1298
FspBI CTAG 3 cut(s) 833, 933, 1352
GlaI GCGC 1 cut(s) 214
GsaI CCCAGC 2 cut(s) 633, 717
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 1 cut(s) 194
HapII CCGG 4 cut(s) 255, 285, 501, 667
HgaI GACGC 1 cut(s) 892
HhaI GCGC 1 cut(s) 215
Hin1I GRCGYC 1 cut(s) 884
Hin1II CATG 5 cut(s) 155, 190, 391, 995, 1143
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HinfI GANTC 4 cut(s) 104, 805, 1039, 1154
HpaII CCGG 4 cut(s) 255, 285, 501, 667
HphI GGTGA 1 cut(s) 156
Hpy166II GTNNAC 2 cut(s) 112, 1411
Hpy188I TCNGA 5 cut(s) 72, 341, 705, 861, 1126
Hpy188III TCNNGA 6 cut(s) 266, 279, 618, 1036, 1166, 1383
Hpy8I GTNNAC 2 cut(s) 112, 1411
HpyAV CCTTC 1 cut(s) 1209
HpyCH4III ACNGT 8 cut(s) 34, 482, 656, 694, 1188, 1222, 1238, 1339
HpyCH4IV ACGT 2 cut(s) 114, 1394
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 53, 497, 605, 796, 914, 938, 1348
HpyF3I CTNAG 9 cut(s) 157, 177, 582, 704, 758, 858, 1049, 1125, 1314
HpySE526I ACGT 2 cut(s) 114, 1394
Hsp92I GRCGYC 1 cut(s) 884
Hsp92II CATG 5 cut(s) 155, 190, 391, 995, 1143
HspAI GCGC 1 cut(s) 213
KspI CCGCGG 1 cut(s) 373
Kzo9I GATC 7 cut(s) 148, 268, 281, 835, 955, 1014, 1210
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 2 cut(s) 227, 930
Lsp1109I GCAGC 9 cut(s) 22, 25, 28, 43, 65, 202, 508, 917, 934
LweI GCATC 8 cut(s) 118, 500, 650, 700, 718, 904, 1356, 1376
MaeI CTAG 3 cut(s) 833, 933, 1352
MaeII ACGT 2 cut(s) 114, 1394
MaeIII GTNAC 3 cut(s) 257, 1262, 1288
MalI GATC 7 cut(s) 150, 270, 283, 837, 957, 1016, 1212
MboI GATC 7 cut(s) 148, 268, 281, 835, 955, 1014, 1210
MboII GAAGA 7 cut(s) 16, 442, 446, 581, 602, 800, 887
MfeI CAATTG 2 cut(s) 732, 1179
MflI RGATCY 3 cut(s) 281, 835, 1014
MhlI GDGCHC 1 cut(s) 753
MmeI TCCRAC 3 cut(s) 291, 704, 863
Mph1103I ATGCAT 1 cut(s) 1391
MroXI GAANNNNTTC 1 cut(s) 1064
MseI TTAA 5 cut(s) 92, 531, 879, 1224, 1272
MslI CAYNNNNRTG 1 cut(s) 657
MspA1I CMGCKG 5 cut(s) 19, 56, 297, 372, 629
MspI CCGG 4 cut(s) 255, 285, 501, 667
MspR9I CCNGG 4 cut(s) 255, 605, 668, 1175
MunI CAATTG 2 cut(s) 732, 1179
Mva1269I GAATGC 3 cut(s) 594, 601, 983
MvaI CCWGG 2 cut(s) 605, 1175
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 8 cut(s) 16, 53, 497, 605, 796, 914, 938, 1348
NciI CCSGG 2 cut(s) 255, 668
NdeII GATC 7 cut(s) 148, 268, 281, 835, 955, 1014, 1210
NlaIII CATG 5 cut(s) 155, 190, 391, 995, 1143
NlaIV GGNNCC 2 cut(s) 283, 1357
NmuCI GTSAC 1 cut(s) 1262
NsiI ATGCAT 1 cut(s) 1391
PciSI GCTCTTC 1 cut(s) 615
PctI GAATGC 3 cut(s) 594, 601, 983
PdmI GAANNNNTTC 1 cut(s) 1064
PfeI GAWTC 4 cut(s) 104, 805, 1039, 1154
PfoI TCCNGGA 1 cut(s) 666
Psp1406I AACGTT 1 cut(s) 114
Psp6I CCWGG 2 cut(s) 603, 1173
PspEI GGTNACC 1 cut(s) 257
PspFI CCCAGC 2 cut(s) 629, 713
PspGI CCWGG 2 cut(s) 603, 1173
PspN4I GGNNCC 2 cut(s) 283, 1357
PspPI GGNCC 1 cut(s) 193
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 3 cut(s) 281, 835, 1014
PvuII CAGCTG 3 cut(s) 19, 56, 297
RsaI GTAC 3 cut(s) 31, 688, 1205
RsaNI GTAC 3 cut(s) 30, 687, 1204
RseI CAYNNNNRTG 1 cut(s) 657
SacII CCGCGG 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 5 cut(s) 92, 531, 879, 1224, 1272
Sau3AI GATC 7 cut(s) 148, 268, 281, 835, 955, 1014, 1210
Sau96I GGNCC 1 cut(s) 193
ScrFI CCNGG 4 cut(s) 255, 605, 668, 1175
SduI GDGCHC 1 cut(s) 753
SfaNI GCATC 8 cut(s) 118, 500, 650, 700, 718, 904, 1356, 1376
Sfr303I CCGCGG 1 cut(s) 373
SgrBI CCGCGG 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 657
SsiI CCGC 4 cut(s) 251, 370, 372, 627
SspMI CTAG 3 cut(s) 833, 933, 1352
StyD4I CCNGG 4 cut(s) 253, 603, 666, 1173
TaaI ACNGT 8 cut(s) 34, 482, 656, 694, 1188, 1222, 1238, 1339
TaiI ACGT 2 cut(s) 117, 1397
TaqI TCGA 5 cut(s) 141, 207, 538, 1213, 1382
TauI GCSGC 1 cut(s) 372
TfiI GAWTC 4 cut(s) 104, 805, 1039, 1154
Tru1I TTAA 5 cut(s) 92, 531, 879, 1224, 1272
Tru9I TTAA 5 cut(s) 92, 531, 879, 1224, 1272
TscAI CASTG 7 cut(s) 39, 114, 659, 699, 1057, 1121, 1191
TseFI GTSAC 1 cut(s) 1262
TseI GCWGC 9 cut(s) 10, 13, 16, 53, 56, 215, 521, 905, 922
Tsp45I GTSAC 1 cut(s) 1262
TspDTI ATGAA 5 cut(s) 17, 366, 990, 1084, 1392
TspRI CASTG 7 cut(s) 39, 114, 659, 699, 1057, 1121, 1191
XapI RAATTY 3 cut(s) 414, 763, 862
XcmI CCANNNNNNNNNTGG 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 1064
XspI CTAG 3 cut(s) 833, 933, 1352
Zsp2I ATGCAT 1 cut(s) 1391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.