AT3G01280

Mitochondrial outer membrane protein porin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003226
Physical Location & Seq
Forward (+)
85551 .. 88070
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G01280.1

Sequence Viewer

Length: 831 bp
ATGGTGAAAGGTCCCGGTCTCTACACCGAAATCGGCAAAAAGGCCAGAGATCTTCTGTACAAAGACCACAACAGTGACCAGAAATTCAGTATCACCACTTTCTCTCCTGCCGGTGTTGCCATCACCTCAACCGGAACTAAGAAAGGTGACTTATTGTTGGGAGATGTCGCTTTTCAGTCGAGGCGAAAGAACATTACTACTGATTTGAAAGTTTGCACTGATTCTACTTTTCTGATCACCGCTACCGTTGATGAGGCTGCACCCGGACTGAGGTCAATCTTCAGCTTCAAGGTTCCTGACCAAAATTCTGGCAAGGTTGAGCTGCAATACTTGCATGAGTACGCCGGTATCAGCACAAGCATGGGATTGACTCAGAACCCAACTGTCAACTTCTCTGGTGTGATTGGCTCCAATGTCTTGGCTGTTGGTACTGATGTTTCATTTGACACCAAGTCTGGAAATTTCACCAAGATCAATGCTGGTTTAAGCTTCACCAAGGAGGACTTAATTGCCTCCCTTACTGTGAACGACAAGGGCGATCTATTGAATGCATCTTACTATCACATTGTGAACCCGCTGTTCAACACTGCGGTTGGAGCCGAAGTGAGCCACAAGTTATCTAGCAAGGACAGCACCATAACTGTTGGAACACAGCACTCGCTTGACCCCTTGACCTCTGTGAAGGCTCGTGTCAATAGTGCGGGTATTGCAAGTGCTCTCATTCAACACGAGTGGAAACCCAAGTCATTCTTCACAATCTCTGGAGAAGTCGACACAAAGTCAATTGACAAGAGTGCTAAAGTTGGATTGGCTCTCGCACTCAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005253 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005773 GO:0005774 GO:0005886 GO:0006091 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008308 GO:0008509 GO:0009060 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010029 GO:0010033 GO:0015075 GO:0015103 GO:0015267 GO:0015318 GO:0015698 GO:0015980 GO:0016020 GO:0016021 GO:0019867 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0030312 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031966 GO:0031967 GO:0031968 GO:0031974 GO:0031975 GO:0031981 GO:0032592 GO:0034220 GO:0042170 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045333 GO:0048580 GO:0050789 GO:0050793 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0055085 GO:0055114 GO:0065007 GO:0070013 GO:0071944 GO:0098542 GO:0098573 GO:0098588 GO:0098656 GO:0098805 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Porin_3

Protein Analysis

276

Amino Acids

29.42

Weight (kDa)

8.77

Isoelectric Point (pI)

18.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 4 - 269 1.8e-81 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 771
AciI CCGC 4 cut(s) 240, 575, 590, 701
AcsI RAATTY 3 cut(s) 83, 304, 460
AcuI CTGAAG 1 cut(s) 265
AdeI CACNNNGTG 1 cut(s) 568
AfaI GTAC 3 cut(s) 59, 341, 430
AfiI CCNNNNNNNGG 1 cut(s) 270
AgsI TTSAA 5 cut(s) 208, 289, 547, 583, 725
AhdI GACNNNNNGTC 2 cut(s) 451, 778
AjuI GAANNNNNNNTTGG 2 cut(s) 734, 766
AleI CACNNNNGTG 1 cut(s) 72
AluBI AGCT 3 cut(s) 285, 322, 489
AluI AGCT 3 cut(s) 285, 322, 489
Alw21I GWGCWC 1 cut(s) 718
Alw26I GTCTC 1 cut(s) 23
AoxI GGCC 1 cut(s) 42
ApeKI GCWGC 2 cut(s) 257, 322
ApoI RAATTY 3 cut(s) 83, 304, 460
AspS9I GGNCC 1 cut(s) 11
AsuC2I CCSGG 2 cut(s) 15, 264
AsuHPI GGTGA 7 cut(s) 16, 85, 115, 158, 229, 457, 484
AvaII GGWCC 1 cut(s) 11
BaeI ACNNNNGTAYC 4 cut(s) 331, 364, 420, 453
BauI CACGAG 2 cut(s) 687, 728
Bbv12I GWGCWC 1 cut(s) 718
BbvI GCAGC 2 cut(s) 244, 309
BccI CCATC 1 cut(s) 128
BclI TGATCA 1 cut(s) 234
BcnI CCSGG 2 cut(s) 15, 264
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 1 cut(s) 621
BglII AGATCT 1 cut(s) 49
BisI GCNGC 2 cut(s) 258, 323
BlsI GCNGC 2 cut(s) 259, 324
Bme1390I CCNGG 2 cut(s) 15, 264
Bme18I GGWCC 1 cut(s) 11
BmeRI GACNNNNNGTC 2 cut(s) 451, 778
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 4 cut(s) 13, 294, 409, 598
BmrFI CCNGG 2 cut(s) 15, 264
BmsI GCATC 1 cut(s) 560
BoxI GACNNNNGTC 1 cut(s) 271
BpmI CTGGAG 1 cut(s) 783
BpuEI CTTGAG 1 cut(s) 806
BpuMI CCSGG 2 cut(s) 15, 264
BsaI GGTCTC 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 495
BsaWI WCCGGW 1 cut(s) 131
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 588, 618
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse118I RCCGGY 2 cut(s) 110, 344
BseDI CCNNGG 1 cut(s) 495
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMII CTCAG 2 cut(s) 260, 386
BseXI GCAGC 2 cut(s) 244, 309
BsgI GTGCAG 1 cut(s) 243
BshFI GGCC 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 718
BsiSI CCGG 5 cut(s) 15, 111, 132, 264, 345
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 23
BsmI GAATGC 1 cut(s) 553
BsnI GGCC 1 cut(s) 44
Bso31I GGTCTC 1 cut(s) 23
Bsp1286I GDGCHC 1 cut(s) 718
Bsp1407I TGTACA 1 cut(s) 57
Bsp143I GATC 4 cut(s) 49, 234, 471, 538
BspACI CCGC 4 cut(s) 240, 575, 590, 701
BspANI GGCC 1 cut(s) 44
BspCNI CTCAG 2 cut(s) 261, 385
BspLI GGNNCC 4 cut(s) 13, 294, 409, 598
BspTNI GGTCTC 1 cut(s) 23
BsrFI RCCGGY 2 cut(s) 110, 344
BsrGI TGTACA 1 cut(s) 57
BssAI RCCGGY 2 cut(s) 110, 344
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 4 cut(s) 49, 234, 471, 538
BssSI CACGAG 2 cut(s) 687, 728
BssT1I CCWWGG 1 cut(s) 495
Bst2BI CACGAG 2 cut(s) 687, 728
Bst4CI ACNGT 5 cut(s) 74, 247, 385, 523, 643
BstAPI GCANNNNNTGC 1 cut(s) 331
BstAUI TGTACA 1 cut(s) 57
BstDEI CTNAG 3 cut(s) 138, 269, 372
BstKTI GATC 4 cut(s) 52, 237, 474, 541
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 4 cut(s) 49, 234, 471, 538
BstMWI GCNNNNNNNGC 5 cut(s) 116, 331, 596, 630, 707
BstPAI GACNNNNGTC 1 cut(s) 271
BstSCI CCNGG 2 cut(s) 13, 262
BstV1I GCAGC 2 cut(s) 244, 309
BstX2I RGATCY 1 cut(s) 49
BstXI CCANNNNNNTGG 2 cut(s) 308, 418
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 1 cut(s) 44
BtsI GCAGTG 1 cut(s) 585
BtsIMutI CAGTG 3 cut(s) 79, 216, 585
Cfr10I RCCGGY 2 cut(s) 110, 344
Cfr13I GGNCC 1 cut(s) 11
Csp6I GTAC 3 cut(s) 58, 340, 429
CspCI CAANNNNNGTGG 2 cut(s) 713, 748
CviAII CATG 2 cut(s) 335, 361
CviQI GTAC 3 cut(s) 58, 340, 429
DdeI CTNAG 3 cut(s) 138, 269, 372
DpnI GATC 4 cut(s) 51, 236, 473, 540
DpnII GATC 4 cut(s) 49, 234, 471, 538
DraIII CACNNNGTG 1 cut(s) 568
DriI GACNNNNNGTC 2 cut(s) 451, 778
Eam1105I GACNNNNNGTC 2 cut(s) 451, 778
Eco130I CCWWGG 1 cut(s) 495
Eco31I GGTCTC 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 11
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 1 cut(s) 553
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 2 cut(s) 338, 364
FaiI YATR 3 cut(s) 336, 362, 638
FalI AAGNNNNNCTT 4 cut(s) 488, 520, 734, 766
FatI CATG 2 cut(s) 334, 360
FauI CCCGC 2 cut(s) 582, 694
FbaI TGATCA 1 cut(s) 234
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 2 cut(s) 258, 323
Fsp4HI GCNGC 2 cut(s) 258, 323
FspBI CTAG 1 cut(s) 621
GluI GCNGC 2 cut(s) 258, 323
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 1 cut(s) 44
HapII CCGG 5 cut(s) 15, 111, 132, 264, 345
Hin1II CATG 2 cut(s) 338, 364
HincII GTYRAC 2 cut(s) 388, 772
HindII GTYRAC 2 cut(s) 388, 772
HindIII AAGCTT 1 cut(s) 487
HinfI GANTC 2 cut(s) 221, 370
HpaII CCGG 5 cut(s) 15, 111, 132, 264, 345
HphI GGTGA 7 cut(s) 16, 85, 115, 158, 229, 457, 484
Hpy166II GTNNAC 4 cut(s) 388, 526, 571, 772
Hpy188I TCNGA 2 cut(s) 234, 375
Hpy188III TCNNGA 3 cut(s) 296, 456, 762
Hpy8I GTNNAC 4 cut(s) 388, 526, 571, 772
HpyAV CCTTC 1 cut(s) 676
HpyCH4III ACNGT 5 cut(s) 74, 247, 385, 523, 643
HpyCH4V TGCA 6 cut(s) 216, 260, 325, 334, 551, 710
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 331, 596, 630, 707
HpyF3I CTNAG 3 cut(s) 138, 269, 372
Hsp92II CATG 2 cut(s) 338, 364
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 4 cut(s) 49, 234, 471, 538
LmnI GCTCC 2 cut(s) 413, 596
Lsp1109I GCAGC 2 cut(s) 244, 309
LweI GCATC 1 cut(s) 560
MaeI CTAG 1 cut(s) 621
MaeIII GTNAC 2 cut(s) 74, 146
MalI GATC 4 cut(s) 51, 236, 473, 540
MboI GATC 4 cut(s) 49, 234, 471, 538
MboII GAAGA 3 cut(s) 44, 271, 742
MfeI CAATTG 1 cut(s) 783
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 718
MluCI AATT 5 cut(s) 83, 304, 460, 507, 783
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 3 cut(s) 574, 625, 784
MnlI CCTC 7 cut(s) 136, 174, 247, 264, 493, 523, 685
Mph1103I ATGCAT 1 cut(s) 553
MseI TTAA 2 cut(s) 485, 506
MslI CAYNNNNRTG 2 cut(s) 72, 359
MspA1I CMGCKG 1 cut(s) 577
MspI CCGG 5 cut(s) 15, 111, 132, 264, 345
MspR9I CCNGG 2 cut(s) 15, 264
MunI CAATTG 1 cut(s) 783
Mva1269I GAATGC 1 cut(s) 553
MwoI GCNNNNNNNGC 5 cut(s) 116, 331, 596, 630, 707
NciI CCSGG 2 cut(s) 15, 264
NdeII GATC 4 cut(s) 49, 234, 471, 538
NlaIII CATG 2 cut(s) 338, 364
NlaIV GGNNCC 4 cut(s) 13, 294, 409, 598
NmuCI GTSAC 2 cut(s) 74, 146
NsiI ATGCAT 1 cut(s) 553
OliI CACNNNNGTG 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 534
PctI GAATGC 1 cut(s) 553
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 2 cut(s) 259, 324
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PpuMI RGGWCCY 1 cut(s) 11
PshAI GACNNNNGTC 1 cut(s) 271
Psp5II RGGWCCY 1 cut(s) 11
PspN4I GGNNCC 4 cut(s) 13, 294, 409, 598
PspPI GGNCC 1 cut(s) 11
PspPPI RGGWCCY 1 cut(s) 11
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 3 cut(s) 59, 341, 430
RsaNI GTAC 3 cut(s) 58, 340, 429
RseI CAYNNNNRTG 2 cut(s) 72, 359
SalI GTCGAC 1 cut(s) 770
SaqAI TTAA 2 cut(s) 485, 506
SatI GCNGC 2 cut(s) 258, 323
Sau3AI GATC 4 cut(s) 49, 234, 471, 538
Sau96I GGNCC 1 cut(s) 11
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 2 cut(s) 15, 264
SduI GDGCHC 1 cut(s) 718
SfaNI GCATC 1 cut(s) 560
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 72, 359
SmlI CTYRAG 1 cut(s) 821
SmoI CTYRAG 1 cut(s) 821
Sse9I AATT 5 cut(s) 83, 304, 460, 507, 783
SsiI CCGC 4 cut(s) 240, 575, 590, 701
SspMI CTAG 1 cut(s) 621
StyD4I CCNGG 2 cut(s) 13, 262
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 5 cut(s) 74, 247, 385, 523, 643
TaqI TCGA 2 cut(s) 179, 771
TasI AATT 5 cut(s) 83, 304, 460, 507, 783
TatI WGTACW 1 cut(s) 57
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 2 cut(s) 485, 506
Tru9I TTAA 2 cut(s) 485, 506
TscAI CASTG 3 cut(s) 79, 223, 592
TseFI GTSAC 2 cut(s) 74, 146
TseI GCWGC 2 cut(s) 257, 322
Tsp45I GTSAC 2 cut(s) 74, 146
TspDTI ATGAA 1 cut(s) 429
TspRI CASTG 3 cut(s) 79, 223, 592
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 3 cut(s) 83, 304, 460
XmiI GTMKAC 1 cut(s) 771
XspI CTAG 1 cut(s) 621
Zsp2I ATGCAT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.