Rroxscaffold_2G00082430

Mitochondrial outer membrane protein porin of 34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003226
Physical Location & Seq
Reverse (-)
5255977 .. 5258451
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082430.1

Sequence Viewer

Length: 831 bp
ATGGCCAAAGGTCCTGGTCTTTACACCGAGATCGGCAAGAAAGCCAGAGATCTCTTGTATAAGGATTACCAGAGCGACCACAAGTTCACCGTCACTACCTACAGTCCCACTGGAGTGGCTATCACTTGTTCAGGAACAAAAAAGGGTGAGCTGTTACTGGCTGATGTTACTACTCAGTTGAAGAACAAAAATATCACTACCGATGTCAAAGTTGACACTGACTCCAATCTTCTCACCACTGTGACCATTGATCAACCTGCTCCTGGCCTCAAGGCAATTCTCAGCTTCAAACTTCCTGATCAGAGGTCAGGCAAGGCGGAACTCCAGTACTTGCATGACTATGCGGGGATAAGCACCAGTGTTGGATTGACAGCCAACCCTGTTGTCAACTTCTCTGGTGTTCTAGGAACTAACCTTCTTGCTCTTGGTACTGATGTGTCATTTGATACCAAAACTGGGAATTTCACCAAATGCAATGCTGGTTTGAGCTTCTCCAATGCAGATCTCATTGCGTCATTGAACCTGAATGAGAAAGGTGATGTGGTGAGTGCATCTTACTATCACAGTGTCTATCCCGAACAAAACACTGCTCTAGGAGCCGAGGTTACCCACAGATTTGCCAACAGTGAGAATACCATCACTCTTGGTGCTCAGCATGCCCTGGATCCATTGACCACTGTCAAGGGACGTGTGAACAACTTTGGCAAGGCCAATGCTCTTATCCAGCATGAGTGGCGCCCAAAATCATTTTTTACTGTTTCTGGAGAGGTAGATACCAGAGCCATTGAGAAGAGCGCCAAGATTGGATTGGCTCTGGCTCTCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005253 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005773 GO:0005774 GO:0005886 GO:0006091 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008308 GO:0008509 GO:0009060 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010029 GO:0010033 GO:0015075 GO:0015103 GO:0015267 GO:0015318 GO:0015698 GO:0015980 GO:0016020 GO:0016021 GO:0019867 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0030312 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031966 GO:0031967 GO:0031968 GO:0031974 GO:0031975 GO:0031981 GO:0032592 GO:0034220 GO:0042170 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045333 GO:0048580 GO:0050789 GO:0050793 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0055085 GO:0055114 GO:0065007 GO:0070013 GO:0071944 GO:0098542 GO:0098573 GO:0098588 GO:0098656 GO:0098805 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Porin_3

Protein Analysis

276

Amino Acids

29.57

Weight (kDa)

8.36

Isoelectric Point (pI)

14.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 4 - 269 1.2e-86 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 265
AccB1I GGYRCC 1 cut(s) 735
AciI CCGC 2 cut(s) 317, 344
AclWI GGATC 2 cut(s) 659, 672
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 460
AcyI GRCGYC 1 cut(s) 736
AfaI GTAC 2 cut(s) 329, 430
AfiI CCNNNNNNNGG 2 cut(s) 263, 456
AflIII ACRYGT 1 cut(s) 688
AgsI TTSAA 3 cut(s) 181, 289, 520
AjiI CACGTC 1 cut(s) 689
AjnI CCWGG 3 cut(s) 13, 262, 660
AleI CACNNNNGTG 2 cut(s) 113, 239
AluBI AGCT 3 cut(s) 151, 285, 489
AluI AGCT 3 cut(s) 151, 285, 489
Alw21I GWGCWC 1 cut(s) 652
AlwI GGATC 2 cut(s) 659, 672
AoxI GGCC 3 cut(s) 3, 265, 708
ApoI RAATTY 1 cut(s) 460
AspLEI GCGC 2 cut(s) 738, 797
AspS9I GGNCC 1 cut(s) 11
AsuHPI GGTGA 6 cut(s) 79, 158, 226, 457, 548, 556
AvaII GGWCC 1 cut(s) 11
BaeI ACNNNNGTAYC 2 cut(s) 420, 453
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 664
BanI GGYRCC 1 cut(s) 735
Bbv12I GWGCWC 1 cut(s) 652
BccI CCATC 1 cut(s) 644
BciT130I CCWGG 3 cut(s) 15, 264, 662
BclI TGATCA 2 cut(s) 250, 298
BfaI CTAG 2 cut(s) 404, 593
BfmI CTRYAG 1 cut(s) 100
BfoI RGCGCY 2 cut(s) 739, 798
BfuAI ACCTGC 1 cut(s) 265
BglII AGATCT 2 cut(s) 49, 502
BlpI GCTNAGC 1 cut(s) 651
BmcAI AGTACT 1 cut(s) 329
Bme1390I CCNGG 3 cut(s) 15, 264, 662
Bme18I GGWCC 1 cut(s) 11
BmgBI CACGTC 1 cut(s) 689
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 3 cut(s) 598, 666, 737
BmrFI CCNGG 3 cut(s) 15, 264, 662
BmrI ACTGGG 1 cut(s) 465
BmsI GCATC 1 cut(s) 560
BmuI ACTGGG 1 cut(s) 465
BoxI GACNNNNGTC 1 cut(s) 677
BpmI CTGGAG 3 cut(s) 132, 308, 783
Bpu1102I GCTNAGC 1 cut(s) 651
BpuEI CTTGAG 2 cut(s) 254, 806
BsaHI GRCGYC 1 cut(s) 736
BsaJI CCNNGG 2 cut(s) 600, 660
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 588, 618
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 456
Bse1I ACTGG 5 cut(s) 115, 162, 325, 357, 460
Bse3DI GCAATG 2 cut(s) 481, 507
BseBI CCWGG 3 cut(s) 15, 264, 662
BseDI CCNNGG 2 cut(s) 600, 660
BseLI CCNNNNNNNGG 2 cut(s) 263, 456
BseMI GCAATG 2 cut(s) 481, 507
BseMII CTCAG 3 cut(s) 188, 295, 665
BseNI ACTGG 5 cut(s) 115, 162, 325, 357, 460
BshFI GGCC 3 cut(s) 5, 267, 710
BshNI GGYRCC 1 cut(s) 735
BsiHKAI GWGCWC 1 cut(s) 652
BslFI GGGAC 2 cut(s) 90, 699
BslI CCNNNNNNNGG 2 cut(s) 263, 456
BsmFI GGGAC 2 cut(s) 90, 699
BsnI GGCC 3 cut(s) 5, 267, 710
Bsp1286I GDGCHC 1 cut(s) 652
Bsp143I GATC 6 cut(s) 30, 49, 250, 298, 502, 664
Bsp1720I GCTNAGC 1 cut(s) 651
BspACI CCGC 2 cut(s) 317, 344
BspANI GGCC 3 cut(s) 5, 267, 710
BspCNI CTCAG 3 cut(s) 187, 294, 664
BspLI GGNNCC 3 cut(s) 598, 666, 737
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 2 cut(s) 659, 672
BspQI GCTCTTC 1 cut(s) 785
BspT107I GGYRCC 1 cut(s) 735
BsrDI GCAATG 2 cut(s) 481, 507
BsrI ACTGG 5 cut(s) 115, 162, 325, 357, 460
BssECI CCNNGG 2 cut(s) 600, 660
BssMI GATC 6 cut(s) 30, 49, 250, 298, 502, 664
BssNI GRCGYC 1 cut(s) 736
Bst2UI CCWGG 3 cut(s) 15, 264, 662
Bst4CI ACNGT 7 cut(s) 91, 104, 241, 566, 626, 679, 757
Bst6I CTCTTC 1 cut(s) 785
BstACI GRCGYC 1 cut(s) 736
BstC8I GCNNGC 1 cut(s) 657
BstDEI CTNAG 3 cut(s) 174, 281, 651
BstEII GGTNACC 1 cut(s) 604
BstH2I RGCGCY 2 cut(s) 739, 798
BstHHI GCGC 2 cut(s) 738, 797
BstKTI GATC 6 cut(s) 33, 52, 253, 301, 505, 667
BstMBI GATC 6 cut(s) 30, 49, 250, 298, 502, 664
BstMWI GCNNNNNNNGC 3 cut(s) 596, 656, 733
BstNI CCWGG 3 cut(s) 15, 264, 662
BstNSI RCATGY 1 cut(s) 659
BstPAI GACNNNNGTC 1 cut(s) 677
BstPI GGTNACC 1 cut(s) 604
BstSCI CCNGG 3 cut(s) 13, 262, 660
BstSFI CTRYAG 1 cut(s) 100
BstX2I RGATCY 3 cut(s) 49, 502, 664
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 3 cut(s) 49, 502, 664
BsuRI GGCC 3 cut(s) 5, 267, 710
BtrI CACGTC 1 cut(s) 689
BtsI GCAGTG 1 cut(s) 585
BtsIMutI CAGTG 8 cut(s) 108, 216, 237, 364, 571, 585, 631, 675
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 1 cut(s) 657
CfoI GCGC 2 cut(s) 738, 797
Cfr13I GGNCC 1 cut(s) 11
CseI GACGC 1 cut(s) 501
Csp6I GTAC 2 cut(s) 328, 429
CviAII CATG 4 cut(s) 335, 656, 728, 828
CviQI GTAC 2 cut(s) 328, 429
DdeI CTNAG 3 cut(s) 174, 281, 651
DinI GGCGCC 1 cut(s) 737
DpnI GATC 6 cut(s) 32, 51, 252, 300, 504, 666
DpnII GATC 6 cut(s) 30, 49, 250, 298, 502, 664
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 785
EarI CTCTTC 1 cut(s) 785
EciI GGCGGA 1 cut(s) 332
Eco47I GGWCC 1 cut(s) 11
Eco91I GGTNACC 1 cut(s) 604
EcoO109I RGGNCCY 1 cut(s) 11
EcoO65I GGTNACC 1 cut(s) 604
EcoRII CCWGG 3 cut(s) 13, 262, 660
EgeI GGCGCC 1 cut(s) 737
EheI GGCGCC 1 cut(s) 737
FaeI CATG 4 cut(s) 338, 659, 731, 831
FaiI YATR 6 cut(s) 60, 336, 342, 657, 729, 829
FaqI GGGAC 2 cut(s) 90, 699
FatI CATG 4 cut(s) 334, 655, 727, 827
FauI CCCGC 1 cut(s) 337
FbaI TGATCA 2 cut(s) 250, 298
FspBI CTAG 2 cut(s) 404, 593
GlaI GCGC 2 cut(s) 737, 796
GsuI CTGGAG 3 cut(s) 132, 308, 783
HaeII RGCGCY 2 cut(s) 739, 798
HaeIII GGCC 3 cut(s) 5, 267, 710
HgaI GACGC 1 cut(s) 501
HhaI GCGC 2 cut(s) 738, 797
Hin1I GRCGYC 1 cut(s) 736
Hin1II CATG 4 cut(s) 338, 659, 731, 831
Hin6I GCGC 2 cut(s) 736, 795
HinP1I GCGC 2 cut(s) 736, 795
HincII GTYRAC 2 cut(s) 214, 388
HindII GTYRAC 2 cut(s) 214, 388
HinfI GANTC 1 cut(s) 221
HphI GGTGA 6 cut(s) 79, 158, 226, 457, 548, 556
Hpy166II GTNNAC 4 cut(s) 87, 214, 388, 694
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 4 cut(s) 132, 296, 575, 762
Hpy8I GTNNAC 4 cut(s) 87, 214, 388, 694
HpyAV CCTTC 1 cut(s) 425
HpyCH4III ACNGT 7 cut(s) 91, 104, 241, 566, 626, 679, 757
HpyCH4IV ACGT 1 cut(s) 688
HpyCH4V TGCA 4 cut(s) 334, 474, 500, 551
HpyF10VI GCNNNNNNNGC 3 cut(s) 596, 656, 733
HpyF3I CTNAG 3 cut(s) 174, 281, 651
HpySE526I ACGT 1 cut(s) 688
Hsp92I GRCGYC 1 cut(s) 736
Hsp92II CATG 4 cut(s) 338, 659, 731, 831
HspAI GCGC 2 cut(s) 736, 795
KasI GGCGCC 1 cut(s) 735
Ksp22I TGATCA 2 cut(s) 250, 298
Kzo9I GATC 6 cut(s) 30, 49, 250, 298, 502, 664
LguI GCTCTTC 1 cut(s) 785
LmnI GCTCC 2 cut(s) 265, 596
LweI GCATC 1 cut(s) 560
MaeI CTAG 2 cut(s) 404, 593
MaeII ACGT 1 cut(s) 688
MaeIII GTNAC 5 cut(s) 91, 153, 166, 241, 604
MalI GATC 6 cut(s) 32, 51, 252, 300, 504, 666
MboI GATC 6 cut(s) 30, 49, 250, 298, 502, 664
MboII GAAGA 3 cut(s) 193, 221, 802
MflI RGATCY 3 cut(s) 49, 502, 664
MhlI GDGCHC 1 cut(s) 652
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 276, 460
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 736
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 4 cut(s) 278, 297, 595, 760
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 3 cut(s) 113, 239, 339
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 3 cut(s) 15, 264, 662
MvaI CCWGG 3 cut(s) 15, 264, 662
MwoI GCNNNNNNNGC 3 cut(s) 596, 656, 733
NarI GGCGCC 1 cut(s) 736
NdeII GATC 6 cut(s) 30, 49, 250, 298, 502, 664
NlaIII CATG 4 cut(s) 338, 659, 731, 831
NlaIV GGNNCC 3 cut(s) 598, 666, 737
NmeAIII GCCGAG 1 cut(s) 625
NmuCI GTSAC 2 cut(s) 91, 241
NspI RCATGY 1 cut(s) 659
OliI CACNNNNGTG 2 cut(s) 113, 239
PaeI GCATGC 1 cut(s) 659
PciSI GCTCTTC 1 cut(s) 785
PleI GAGTC 1 cut(s) 215
PluTI GGCGCC 1 cut(s) 739
PpsI GAGTC 1 cut(s) 215
PpuMI RGGWCCY 1 cut(s) 11
PshAI GACNNNNGTC 1 cut(s) 677
Psp5II RGGWCCY 1 cut(s) 11
Psp6I CCWGG 3 cut(s) 13, 262, 660
PspEI GGTNACC 1 cut(s) 604
PspGI CCWGG 3 cut(s) 13, 262, 660
PspN4I GGNNCC 3 cut(s) 598, 666, 737
PspPI GGNCC 1 cut(s) 11
PspPPI RGGWCCY 1 cut(s) 11
PsuI RGATCY 3 cut(s) 49, 502, 664
RsaI GTAC 2 cut(s) 329, 430
RsaNI GTAC 2 cut(s) 328, 429
RseI CAYNNNNRTG 3 cut(s) 113, 239, 339
SapI GCTCTTC 1 cut(s) 785
Sau3AI GATC 6 cut(s) 30, 49, 250, 298, 502, 664
Sau96I GGNCC 1 cut(s) 11
ScaI AGTACT 1 cut(s) 329
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 3 cut(s) 15, 264, 662
SduI GDGCHC 1 cut(s) 652
SfaNI GCATC 1 cut(s) 560
SfcI CTRYAG 1 cut(s) 100
SfoI GGCGCC 1 cut(s) 737
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 3 cut(s) 113, 239, 339
SmlI CTYRAG 2 cut(s) 269, 821
SmoI CTYRAG 2 cut(s) 269, 821
SphI GCATGC 1 cut(s) 659
Sse9I AATT 2 cut(s) 276, 460
SsiI CCGC 2 cut(s) 317, 344
SspDI GGCGCC 1 cut(s) 735
SspMI CTAG 2 cut(s) 404, 593
StyD4I CCNGG 3 cut(s) 13, 262, 660
TaaI ACNGT 7 cut(s) 91, 104, 241, 566, 626, 679, 757
TaiI ACGT 1 cut(s) 691
TasI AATT 2 cut(s) 276, 460
TatI WGTACW 1 cut(s) 327
TscAI CASTG 8 cut(s) 115, 223, 244, 364, 571, 592, 631, 682
TseFI GTSAC 2 cut(s) 91, 241
Tsp45I GTSAC 2 cut(s) 91, 241
TspRI CASTG 8 cut(s) 115, 223, 244, 364, 571, 592, 631, 682
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 1 cut(s) 460
XceI RCATGY 1 cut(s) 659
XcmI CCANNNNNNNNNTGG 1 cut(s) 805
XspI CTAG 2 cut(s) 404, 593
ZrmI AGTACT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.