MD09G1047200.v1.1

Mitochondrial outer membrane protein porin of 34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003226
Physical Location & Seq
Reverse (-)
3093747 .. 3096449
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1047200.v1.1.491

Sequence Viewer

Length: 828 bp
ATGGTGGGACCTGGGCTTTACACCGACATCGGCAAGAAAGCCAGAGATCTTCTGTACAGGGATTACCAGAGCGACCATAAGTTCACCATCACCACCTTCTCTCCCACTGGAGTGGCTATCACTTCTTCAGGAACGAAGAAAGGTGAGTTGTTTCTGGCTGATGTGAACACTCAGTTGAAGAACAAGAATGTCACAACTGATGTCAAAGTTGACACCGACTCCAATCTGTCCACCACTATTACGGTTGATCAACCTGCTCCTGGCCTGAAGGCAATCTTTAGCTTCAAAGTTCCTGATCAGAGGTCTGGCAAGGTGGAACTCCAATATCTGCATGACTATGCAGGGATAAGCTCCAGTGTTGGTTTGACAGCGAACCCTATTGTTAACTTCTCCGGTGTTATTGGAACCAATCAGCTTGCACTTGGTACTGATCTGTCATTCGACACCAAGACCGGGAATTTGACTAAATGCAATGCTGGACTGAGCTTCTCCAATGCTGATCTGGTCGCTGCATTGACCCTGAATGATAAAGGTGACATCCTCAGTGCATCCTACTATCACAGTGTCAATCCCCTTTCCAACACAGCTGTTGGCGCTGAGGTGACCCACAGGTTTCCTACCAACGAGAATACCATCACTCTTGGCACCCAGCATGCCCTGGATCCATTGACATCCATTAAGGCACGCGTAAACAACTTTGGCAAAGCAAGTGCCCTGATCCAGCACGAGTGGCGCCCAAAGTCATTCCTCACTGTTTCAGGAGAGGTAGACACCAAGGCCATTGAGAAGAGCGCCAAGATTGGGTTGGCTCTTGCTCTCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005253 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005773 GO:0005774 GO:0005886 GO:0006091 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008308 GO:0008509 GO:0009060 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010029 GO:0010033 GO:0015075 GO:0015103 GO:0015267 GO:0015318 GO:0015698 GO:0015980 GO:0016020 GO:0016021 GO:0019867 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0030312 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031966 GO:0031967 GO:0031968 GO:0031974 GO:0031975 GO:0031981 GO:0032592 GO:0034220 GO:0042170 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045333 GO:0048580 GO:0050789 GO:0050793 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0055085 GO:0055114 GO:0065007 GO:0070013 GO:0071944 GO:0098542 GO:0098573 GO:0098588 GO:0098656 GO:0098805 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Porin_3

Protein Analysis

276

Amino Acids

29.36

Weight (kDa)

8.48

Isoelectric Point (pI)

9.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 3 - 268 8.1e-89 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 262
AccB1I GGYRCC 2 cut(s) 644, 732
AccI GTMKAC 1 cut(s) 768
AccII CGCG 1 cut(s) 687
AclWI GGATC 3 cut(s) 656, 669, 712
AcsI RAATTY 1 cut(s) 457
AcuI CTGAAG 2 cut(s) 111, 287
AcyI GRCGYC 1 cut(s) 733
AfaI GTAC 2 cut(s) 56, 427
AfiI CCNNNNNNNGG 3 cut(s) 260, 453, 801
AflIII ACRYGT 1 cut(s) 685
AgsI TTSAA 2 cut(s) 178, 286
AjnI CCWGG 3 cut(s) 10, 259, 657
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 5 cut(s) 282, 351, 415, 486, 587
AluI AGCT 5 cut(s) 282, 351, 415, 486, 587
AlwI GGATC 3 cut(s) 656, 669, 712
AoxI GGCC 2 cut(s) 262, 777
ApeKI GCWGC 1 cut(s) 509
ApoI RAATTY 1 cut(s) 457
ArsI GACNNNNNNTTYG 2 cut(s) 442, 474
AspLEI GCGC 3 cut(s) 596, 735, 794
AspS9I GGNCC 1 cut(s) 8
AsuC2I CCSGG 1 cut(s) 454
AsuHPI GGTGA 5 cut(s) 76, 82, 155, 545, 613
AvaII GGWCC 1 cut(s) 8
BaeGI GKGCMC 1 cut(s) 715
BamHI GGATCC 1 cut(s) 661
BanI GGYRCC 2 cut(s) 644, 732
BauI CACGAG 1 cut(s) 725
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 1 cut(s) 496
BccI CCATC 2 cut(s) 95, 641
BciT130I CCWGG 3 cut(s) 12, 261, 659
BclI TGATCA 2 cut(s) 247, 295
BcnI CCSGG 1 cut(s) 454
BfoI RGCGCY 3 cut(s) 597, 736, 795
BfuAI ACCTGC 1 cut(s) 262
BglII AGATCT 1 cut(s) 46
BisI GCNGC 1 cut(s) 510
BlsI GCNGC 1 cut(s) 511
Bme1390I CCNGG 4 cut(s) 12, 261, 454, 659
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 5 cut(s) 9, 406, 646, 663, 734
BmrFI CCNGG 4 cut(s) 12, 261, 454, 659
BmsI GCATC 1 cut(s) 557
BpmI CTGGAG 2 cut(s) 129, 337
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 1 cut(s) 803
BpuMI CCSGG 1 cut(s) 454
BsaHI GRCGYC 1 cut(s) 733
BsaJI CCNNGG 3 cut(s) 11, 657, 774
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 203, 233
Bsc4I CCNNNNNNNGG 3 cut(s) 260, 453, 801
Bse1I ACTGG 2 cut(s) 112, 354
Bse3DI GCAATG 1 cut(s) 478
BseBI CCWGG 3 cut(s) 12, 261, 659
BseDI CCNNGG 3 cut(s) 11, 657, 774
BseGI GGATG 3 cut(s) 537, 548, 671
BseLI CCNNNNNNNGG 3 cut(s) 260, 453, 801
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 4 cut(s) 185, 473, 556, 588
BseNI ACTGG 2 cut(s) 112, 354
BseSI GKGCMC 1 cut(s) 715
BseXI GCAGC 1 cut(s) 496
BseYI CCCAGC 1 cut(s) 648
Bsh1236I CGCG 1 cut(s) 687
BshFI GGCC 2 cut(s) 264, 779
BshNI GGYRCC 2 cut(s) 644, 732
BsiSI CCGG 2 cut(s) 393, 453
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 3 cut(s) 260, 453, 801
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 2 cut(s) 264, 779
Bsp1286I GDGCHC 1 cut(s) 715
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 7 cut(s) 46, 247, 295, 430, 499, 661, 717
BspANI GGCC 2 cut(s) 264, 779
BspCNI CTCAG 4 cut(s) 184, 474, 555, 589
BspFNI CGCG 1 cut(s) 687
BspLI GGNNCC 5 cut(s) 9, 406, 646, 663, 734
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 3 cut(s) 656, 669, 712
BspQI GCTCTTC 1 cut(s) 782
BspT107I GGYRCC 2 cut(s) 644, 732
BsrDI GCAATG 1 cut(s) 478
BsrGI TGTACA 1 cut(s) 54
BsrI ACTGG 2 cut(s) 112, 354
BssECI CCNNGG 3 cut(s) 11, 657, 774
BssMI GATC 7 cut(s) 46, 247, 295, 430, 499, 661, 717
BssNI GRCGYC 1 cut(s) 733
BssSI CACGAG 1 cut(s) 725
BssT1I CCWWGG 1 cut(s) 774
Bst2BI CACGAG 1 cut(s) 725
Bst2UI CCWGG 3 cut(s) 12, 261, 659
Bst4CI ACNGT 3 cut(s) 244, 563, 754
Bst6I CTCTTC 1 cut(s) 782
BstACI GRCGYC 1 cut(s) 733
BstAUI TGTACA 1 cut(s) 54
BstC8I GCNNGC 3 cut(s) 417, 654, 685
BstDEI CTNAG 4 cut(s) 171, 482, 542, 597
BstEII GGTNACC 1 cut(s) 601
BstF5I GGATG 3 cut(s) 537, 548, 671
BstFNI CGCG 1 cut(s) 687
BstH2I RGCGCY 3 cut(s) 597, 736, 795
BstHHI GCGC 3 cut(s) 596, 735, 794
BstKTI GATC 7 cut(s) 49, 250, 298, 433, 502, 664, 720
BstMBI GATC 7 cut(s) 46, 247, 295, 430, 499, 661, 717
BstMWI GCNNNNNNNGC 2 cut(s) 593, 730
BstNI CCWGG 3 cut(s) 12, 261, 659
BstNSI RCATGY 1 cut(s) 656
BstPI GGTNACC 1 cut(s) 601
BstSCI CCNGG 4 cut(s) 10, 259, 452, 657
BstSLI GKGCMC 1 cut(s) 715
BstUI CGCG 1 cut(s) 687
BstV1I GCAGC 1 cut(s) 496
BstX2I RGATCY 2 cut(s) 46, 661
BstXI CCANNNNNNTGG 1 cut(s) 112
BstYI RGATCY 2 cut(s) 46, 661
BsuRI GGCC 2 cut(s) 264, 779
BtsCI GGATG 3 cut(s) 537, 548, 671
BtsIMutI CAGTG 5 cut(s) 105, 361, 550, 568, 750
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 3 cut(s) 417, 654, 685
CfoI GCGC 3 cut(s) 596, 735, 794
Cfr13I GGNCC 1 cut(s) 8
Csp6I GTAC 2 cut(s) 55, 426
CviAII CATG 3 cut(s) 332, 653, 825
CviQI GTAC 2 cut(s) 55, 426
DdeI CTNAG 4 cut(s) 171, 482, 542, 597
DinI GGCGCC 1 cut(s) 734
DpnI GATC 7 cut(s) 48, 249, 297, 432, 501, 663, 719
DpnII GATC 7 cut(s) 46, 247, 295, 430, 499, 661, 717
Eam1104I CTCTTC 1 cut(s) 782
EarI CTCTTC 1 cut(s) 782
Eco130I CCWWGG 1 cut(s) 774
Eco47I GGWCC 1 cut(s) 8
Eco57I CTGAAG 2 cut(s) 111, 287
Eco91I GGTNACC 1 cut(s) 601
EcoO109I RGGNCCY 1 cut(s) 8
EcoO65I GGTNACC 1 cut(s) 601
EcoRII CCWGG 3 cut(s) 10, 259, 657
EcoT14I CCWWGG 1 cut(s) 774
EgeI GGCGCC 1 cut(s) 734
EheI GGCGCC 1 cut(s) 734
ErhI CCWWGG 1 cut(s) 774
FaeI CATG 3 cut(s) 335, 656, 828
FaiI YATR 5 cut(s) 78, 333, 339, 654, 826
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FaqI GGGAC 1 cut(s) 21
FatI CATG 3 cut(s) 331, 652, 824
FbaI TGATCA 2 cut(s) 247, 295
FblI GTMKAC 1 cut(s) 768
Fnu4HI GCNGC 1 cut(s) 510
FokI GGATG 3 cut(s) 524, 535, 658
Fsp4HI GCNGC 1 cut(s) 510
GlaI GCGC 3 cut(s) 595, 734, 793
GluI GCNGC 1 cut(s) 510
GsaI CCCAGC 1 cut(s) 652
GsuI CTGGAG 2 cut(s) 129, 337
HaeII RGCGCY 3 cut(s) 597, 736, 795
HaeIII GGCC 2 cut(s) 264, 779
HapII CCGG 2 cut(s) 393, 453
HhaI GCGC 3 cut(s) 596, 735, 794
Hin1I GRCGYC 1 cut(s) 733
Hin1II CATG 3 cut(s) 335, 656, 828
Hin6I GCGC 3 cut(s) 594, 733, 792
HinP1I GCGC 3 cut(s) 594, 733, 792
HincII GTYRAC 2 cut(s) 211, 385
HindII GTYRAC 2 cut(s) 211, 385
HinfI GANTC 1 cut(s) 218
HpaI GTTAAC 1 cut(s) 385
HpaII CCGG 2 cut(s) 393, 453
HphI GGTGA 5 cut(s) 76, 82, 155, 545, 613
Hpy166II GTNNAC 7 cut(s) 84, 166, 211, 231, 385, 691, 769
Hpy188I TCNGA 1 cut(s) 300
Hpy188III TCNNGA 3 cut(s) 129, 293, 759
Hpy8I GTNNAC 7 cut(s) 84, 166, 211, 231, 385, 691, 769
HpyAV CCTTC 2 cut(s) 106, 262
HpyCH4III ACNGT 3 cut(s) 244, 563, 754
HpyCH4V TGCA 6 cut(s) 331, 341, 419, 471, 512, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 593, 730
HpyF3I CTNAG 4 cut(s) 171, 482, 542, 597
Hsp92I GRCGYC 1 cut(s) 733
Hsp92II CATG 3 cut(s) 335, 656, 828
HspAI GCGC 3 cut(s) 594, 733, 792
KasI GGCGCC 1 cut(s) 732
Ksp22I TGATCA 2 cut(s) 247, 295
KspAI GTTAAC 1 cut(s) 385
Kzo9I GATC 7 cut(s) 46, 247, 295, 430, 499, 661, 717
LguI GCTCTTC 1 cut(s) 782
LmnI GCTCC 2 cut(s) 262, 356
Lsp1109I GCAGC 1 cut(s) 496
LweI GCATC 1 cut(s) 557
MaeIII GTNAC 3 cut(s) 190, 533, 601
MalI GATC 7 cut(s) 48, 249, 297, 432, 501, 663, 719
MboI GATC 7 cut(s) 46, 247, 295, 430, 499, 661, 717
MboII GAAGA 5 cut(s) 41, 117, 148, 190, 799
MflI RGATCY 2 cut(s) 46, 661
MhlI GDGCHC 1 cut(s) 715
MluCI AATT 1 cut(s) 457
MluI ACGCGT 1 cut(s) 685
Mly113I GGCGCC 1 cut(s) 733
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 1 cut(s) 603
MnlI CCTC 5 cut(s) 294, 551, 592, 757, 758
MseI TTAA 2 cut(s) 384, 678
MslI CAYNNNNRTG 2 cut(s) 110, 336
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 2 cut(s) 393, 453
MspR9I CCNGG 4 cut(s) 12, 261, 454, 659
MvaI CCWGG 3 cut(s) 12, 261, 659
MvnI CGCG 1 cut(s) 687
MwoI GCNNNNNNNGC 2 cut(s) 593, 730
NarI GGCGCC 1 cut(s) 733
NciI CCSGG 1 cut(s) 454
NdeII GATC 7 cut(s) 46, 247, 295, 430, 499, 661, 717
NlaIII CATG 3 cut(s) 335, 656, 828
NlaIV GGNNCC 5 cut(s) 9, 406, 646, 663, 734
NmuCI GTSAC 3 cut(s) 190, 533, 601
NspI RCATGY 1 cut(s) 656
OliI CACNNNNGTG 1 cut(s) 110
PaeI GCATGC 1 cut(s) 656
PciSI GCTCTTC 1 cut(s) 782
PkrI GCNGC 1 cut(s) 511
PleI GAGTC 1 cut(s) 212
PluTI GGCGCC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 212
PpuMI RGGWCCY 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 8
Psp6I CCWGG 3 cut(s) 10, 259, 657
PspEI GGTNACC 1 cut(s) 601
PspFI CCCAGC 1 cut(s) 648
PspGI CCWGG 3 cut(s) 10, 259, 657
PspN4I GGNNCC 5 cut(s) 9, 406, 646, 663, 734
PspPI GGNCC 1 cut(s) 8
PspPPI RGGWCCY 1 cut(s) 8
PsuI RGATCY 2 cut(s) 46, 661
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 2 cut(s) 56, 427
RsaNI GTAC 2 cut(s) 55, 426
RseI CAYNNNNRTG 2 cut(s) 110, 336
SapI GCTCTTC 1 cut(s) 782
SaqAI TTAA 2 cut(s) 384, 678
SatI GCNGC 1 cut(s) 510
Sau3AI GATC 7 cut(s) 46, 247, 295, 430, 499, 661, 717
Sau96I GGNCC 1 cut(s) 8
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 4 cut(s) 12, 261, 454, 659
SduI GDGCHC 1 cut(s) 715
SfaNI GCATC 1 cut(s) 557
SfoI GGCGCC 1 cut(s) 734
SinI GGWCC 1 cut(s) 8
SmiMI CAYNNNNRTG 2 cut(s) 110, 336
SmlI CTYRAG 1 cut(s) 818
SmoI CTYRAG 1 cut(s) 818
SphI GCATGC 1 cut(s) 656
Sse9I AATT 1 cut(s) 457
SspDI GGCGCC 1 cut(s) 732
StyD4I CCNGG 4 cut(s) 10, 259, 452, 657
StyI CCWWGG 1 cut(s) 774
TaaI ACNGT 3 cut(s) 244, 563, 754
TaqI TCGA 1 cut(s) 441
TasI AATT 1 cut(s) 457
TatI WGTACW 1 cut(s) 54
Tru1I TTAA 2 cut(s) 384, 678
Tru9I TTAA 2 cut(s) 384, 678
TscAI CASTG 5 cut(s) 112, 361, 550, 568, 757
TseFI GTSAC 3 cut(s) 190, 533, 601
TseI GCWGC 1 cut(s) 509
Tsp45I GTSAC 3 cut(s) 190, 533, 601
TspRI CASTG 5 cut(s) 112, 361, 550, 568, 757
VpaK11BI GGWCC 1 cut(s) 8
XapI RAATTY 1 cut(s) 457
XceI RCATGY 1 cut(s) 656
XcmI CCANNNNNNNNNTGG 2 cut(s) 499, 802
XmiI GTMKAC 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.