Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003226

Physical Location & Seq

Genomic Coordinates Reverse (-)

5493594 .. 5496507

Gene Structure 2914 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1078300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 831 bp

ATGGTGAAGGGCCCAGGACTCTACTTCGATATCGGCAAGAAAGCCAGAGATCTTCTCTACAAGGATTACCAGAGCGACCACAAGTTCACCGTCACCACTTACACTTCCACCGGAGTTGCAATCAGTTCAACTGGAATCAGGAAGGGTGAACTGTATCTTGGGGATGTGAGCACTCAGCTGAAGAACAAGAACATCACGACTGACGTGAAAGTTGACACCAACTCTAATCTTCTCACGACCATTACTATTGATGAACCTGCACCTGGTCTCAAGGCAATCTTTAGCTTTATTGCCCCTGACCAGAGATCTGGCAAGGTGGAGCTCCAATATCAGCATGAGTATGCCGGGATAAGCACTAGCATTGGGTTGACTGCCAATCCGATTGTTAACTTTTCTGGTGTTGTGGGGAACAATCTTCTCTCTCTGGGAACTGATCTTTCTTTCGACACTGCCTCTGGCAACTTTACCAAAGTGAATGCAGGGTTGAATTTCACCCATTCTGACCTCATTGCTTCCCTATTACTGAACGATAAAGCTGATACTATTACTGCTTCCTACTACCACACTGTAAGCCCGCTCACCAACACTGCTGTTGGTGCGGAGCTGTCCCATAGCTTTTCAAGCAATGAAAACAGCCTCACAATTGGCACAGCACATGCACTTGACCCCCTAACCACAGTGAAGGCTCGGGTGAACAACTATGGCAGGGCGAGCGCTCTCATCCAGCACGAGTGGCGTCCCAAGTCATTCTTCACCATCTCAGGAGAGGTTGATACCAGGGCAATAGAGAAGAGCGCAAAGATCGGTCTAGCCTTGGCCCTCAAGCCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005253 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008308 GO:0008509 GO:0009526 GO:0009527 GO:0009536 GO:0015075 GO:0015103 GO:0015267 GO:0015318 GO:0015698 GO:0016020 GO:0016021 GO:0019867 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032592 GO:0034220 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044435 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098573 GO:0098588 GO:0098656 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166

Pfam Domains

Protein Families

Porin_3

Protein Analysis

277

Amino Acids

29.65

Weight (kDa)

7.06

Isoelectric Point (pI)

10.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Porin_3	PF01459	4 - 269	5.5e-87	Eukaryotic porin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003226)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01280 AT5G15090 AT5G15090
fragaria_vesca	FvH4_4g16150 FvH4_4g31911 FvH4_6g49250
malus_domestica	MD09G1047200.v1.1 MD13G1078700.v1.1 MD16G1078300.v1.1 MD17G1047400.v1.1
prunus_persica	Prupe.1G268200_v2.0.a1 Prupe.3G271800_v2.0.a1
pyrus_communis	pycom111g03800 pycom13g06830 pycom16g06780 pycom17g04220
rosa_chinensis	RchiOBHm_Chr2g0169111 RchiOBHm_Chr4g0440571
rosa_laevigata	RLG00000006167 RLG00000021835
rosa_multiflora	Rmu_sc0011312.1_g000001
rosa_roxburghii	Rroxscaffold_2G00082430 Rroxscaffold_5G00381400
rosa_rugosa	Rorug02G0537000 Rorug04G0326700
rosa_samantha	Rh2AG608900 Rh2BG621800 Rh2CG590500 Rh2DG632400 Rh4AG378500 Rh4AG378600 Rh4BG391200 Rh4CG405900 Rh4DG385100
rosa_wichuraiana	Rw2G050490 Rw4G032410 Rw4G032510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	265
AccBSI	CCGCTC	1 cut(s)	577
AciI	CCGC	2 cut(s)	575, 599
AcsI	RAATTY	1 cut(s)	487
AcuI	CTGAAG	1 cut(s)	200
AcyI	GRCGYC	1 cut(s)	736
AfeI	AGCGCT	1 cut(s)	715
AfiI	CCNNNNNNNGG	1 cut(s)	263
AgsI	TTSAA	3 cut(s)	129, 487, 621
AjiI	CACGTC	1 cut(s)	205
AjnI	CCWGG	3 cut(s)	13, 262, 776
AjuI	GAANNNNNNNTTGG	4 cut(s)	141, 173, 734, 766
AluBI	AGCT	6 cut(s)	178, 285, 322, 536, 604, 615
AluI	AGCT	6 cut(s)	178, 285, 322, 536, 604, 615
Alw21I	GWGCWC	2 cut(s)	173, 324
Alw26I	GTCTC	1 cut(s)	272
Ama87I	CYCGRG	1 cut(s)	687
Aor51HI	AGCGCT	1 cut(s)	715
AoxI	GGCC	2 cut(s)	10, 816
ApaI	GGGCCC	1 cut(s)	14
ApoI	RAATTY	1 cut(s)	487
AspLEI	GCGC	2 cut(s)	716, 797
AspS9I	GGNCC	3 cut(s)	10, 11, 817
AsuC2I	CCSGG	1 cut(s)	346
AsuHPI	GGTGA	8 cut(s)	16, 79, 85, 158, 484, 571, 703, 745
AvaI	CYCGRG	1 cut(s)	687
BaeGI	GKGCMC	1 cut(s)	14
BanII	GRGCYC	2 cut(s)	14, 324
BauI	CACGAG	1 cut(s)	728
Bbv12I	GWGCWC	2 cut(s)	173, 324
BccI	CCATC	1 cut(s)	764
BciT130I	CCWGG	3 cut(s)	15, 264, 778
BcnI	CCSGG	1 cut(s)	346
BcoDI	GTCTC	1 cut(s)	272
BfaI	CTAG	3 cut(s)	357, 809, 829
BfoI	RGCGCY	1 cut(s)	717
BfuAI	ACCTGC	1 cut(s)	265
BglII	AGATCT	2 cut(s)	49, 305
Bme1390I	CCNGG	4 cut(s)	15, 264, 346, 778
BmeT110I	CYCGRG	1 cut(s)	687
BmgBI	CACGTC	1 cut(s)	205
BmgT120I	GGNCC	3 cut(s)	10, 11, 817
BmiI	GGNNCC	1 cut(s)	12
BmrFI	CCNGG	4 cut(s)	15, 264, 346, 778
BplI	GAGNNNNNCTC	2 cut(s)	39, 71
BpuEI	CTTGAG	2 cut(s)	254, 806
BpuMI	CCSGG	1 cut(s)	346
BsaHI	GRCGYC	1 cut(s)	736
BsaI	GGTCTC	1 cut(s)	272
BsaJI	CCNNGG	3 cut(s)	13, 777, 813
BsaWI	WCCGGW	1 cut(s)	110
Bsc4I	CCNNNNNNNGG	1 cut(s)	263
Bse1I	ACTGG	1 cut(s)	136
Bse3DI	GCAATG	2 cut(s)	507, 631
BseBI	CCWGG	3 cut(s)	15, 264, 778
BseDI	CCNNGG	3 cut(s)	13, 777, 813
BseGI	GGATG	2 cut(s)	169, 720
BseLI	CCNNNNNNNGG	1 cut(s)	263
BseMI	GCAATG	2 cut(s)	507, 631
BseMII	CTCAG	2 cut(s)	188, 774
BseNI	ACTGG	1 cut(s)	136
BseSI	GKGCMC	1 cut(s)	14
BsgI	GTGCAG	1 cut(s)	243
BshFI	GGCC	2 cut(s)	12, 818
BsiHKAI	GWGCWC	2 cut(s)	173, 324
BsiHKCI	CYCGRG	1 cut(s)	687
BsiSI	CCGG	2 cut(s)	111, 345
BslFI	GGGAC	2 cut(s)	592, 723
BslI	CCNNNNNNNGG	1 cut(s)	263
BsmAI	GTCTC	1 cut(s)	272
BsmFI	GGGAC	2 cut(s)	592, 723
BsmI	GAATGC	1 cut(s)	481
BsnI	GGCC	2 cut(s)	12, 818
Bso31I	GGTCTC	1 cut(s)	272
BsoBI	CYCGRG	1 cut(s)	687
Bsp120I	GGGCCC	1 cut(s)	10
Bsp1286I	GDGCHC	3 cut(s)	14, 173, 324
Bsp143I	GATC	4 cut(s)	49, 305, 433, 801
BspACI	CCGC	2 cut(s)	575, 599
BspANI	GGCC	2 cut(s)	12, 818
BspCNI	CTCAG	2 cut(s)	187, 773
BspLI	GGNNCC	1 cut(s)	12
BspMI	ACCTGC	1 cut(s)	265
BspQI	GCTCTTC	1 cut(s)	785
BspTNI	GGTCTC	1 cut(s)	272
BsrBI	CCGCTC	1 cut(s)	577
BsrDI	GCAATG	2 cut(s)	507, 631
BsrI	ACTGG	1 cut(s)	136
BssECI	CCNNGG	3 cut(s)	13, 777, 813
BssMI	GATC	4 cut(s)	49, 305, 433, 801
BssNI	GRCGYC	1 cut(s)	736
BssSI	CACGAG	1 cut(s)	728
BssT1I	CCWWGG	1 cut(s)	813
Bst2BI	CACGAG	1 cut(s)	728
Bst2UI	CCWGG	3 cut(s)	15, 264, 778
Bst4CI	ACNGT	4 cut(s)	91, 153, 568, 679
Bst6I	CTCTTC	1 cut(s)	785
BstACI	GRCGYC	1 cut(s)	736
BstC8I	GCNNGC	2 cut(s)	575, 712
BstDEI	CTNAG	2 cut(s)	174, 760
BstF5I	GGATG	2 cut(s)	169, 720
BstH2I	RGCGCY	1 cut(s)	717
BstHHI	GCGC	2 cut(s)	716, 797
BstKTI	GATC	4 cut(s)	52, 308, 436, 804
BstMAI	GTCTC	1 cut(s)	272
BstMBI	GATC	4 cut(s)	49, 305, 433, 801
BstMWI	GCNNNNNNNGC	4 cut(s)	596, 621, 711, 733
BstNI	CCWGG	3 cut(s)	15, 264, 778
BstNSI	RCATGY	1 cut(s)	659
BstSCI	CCNGG	4 cut(s)	13, 262, 344, 776
BstSLI	GKGCMC	1 cut(s)	14
BstX2I	RGATCY	2 cut(s)	49, 305
BstXI	CCANNNNNNTGG	1 cut(s)	308
BstYI	RGATCY	2 cut(s)	49, 305
BsuRI	GGCC	2 cut(s)	12, 818
BtrI	CACGTC	1 cut(s)	205
BtsCI	GGATG	2 cut(s)	169, 720
BtsI	GCAGTG	2 cut(s)	447, 585
BtsIMutI	CAGTG	4 cut(s)	447, 564, 585, 684
BveI	ACCTGC	1 cut(s)	265
Cac8I	GCNNGC	2 cut(s)	575, 712
CfoI	GCGC	2 cut(s)	716, 797
Cfr13I	GGNCC	3 cut(s)	10, 11, 817
CseI	GACGC	1 cut(s)	725
CsiI	ACCWGGT	1 cut(s)	262
CspCI	CAANNNNNGTGG	2 cut(s)	97, 132
CviAII	CATG	2 cut(s)	335, 656
DdeI	CTNAG	2 cut(s)	174, 760
DpnI	GATC	4 cut(s)	51, 307, 435, 803
DpnII	GATC	4 cut(s)	49, 305, 433, 801
Eam1104I	CTCTTC	1 cut(s)	785
EarI	CTCTTC	1 cut(s)	785
Ecl136II	GAGCTC	1 cut(s)	322
Eco130I	CCWWGG	1 cut(s)	813
Eco24I	GRGCYC	2 cut(s)	14, 324
Eco31I	GGTCTC	1 cut(s)	272
Eco32I	GATATC	1 cut(s)	31
Eco47III	AGCGCT	1 cut(s)	715
Eco53kI	GAGCTC	1 cut(s)	322
Eco57I	CTGAAG	1 cut(s)	200
Eco88I	CYCGRG	1 cut(s)	687
EcoICRI	GAGCTC	1 cut(s)	322
EcoO109I	RGGNCCY	1 cut(s)	10
EcoRII	CCWGG	3 cut(s)	13, 262, 776
EcoRV	GATATC	1 cut(s)	31
EcoT14I	CCWWGG	1 cut(s)	813
EcoT38I	GRGCYC	2 cut(s)	14, 324
ErhI	CCWWGG	1 cut(s)	813
FaeI	CATG	2 cut(s)	338, 659
FaiI	YATR	5 cut(s)	336, 342, 612, 657, 702
FalI	AAGNNNNNCTT	4 cut(s)	263, 295, 734, 766
FaqI	GGGAC	2 cut(s)	592, 723
FatI	CATG	2 cut(s)	334, 655
FauI	CCCGC	1 cut(s)	582
FokI	GGATG	2 cut(s)	176, 707
FriOI	GRGCYC	2 cut(s)	14, 324
FspBI	CTAG	3 cut(s)	357, 809, 829
GlaI	GCGC	2 cut(s)	715, 796
HaeII	RGCGCY	1 cut(s)	717
HaeIII	GGCC	2 cut(s)	12, 818
HapII	CCGG	2 cut(s)	111, 345
HgaI	GACGC	1 cut(s)	725
HhaI	GCGC	2 cut(s)	716, 797
Hin1I	GRCGYC	1 cut(s)	736
Hin1II	CATG	2 cut(s)	338, 659
Hin6I	GCGC	2 cut(s)	714, 795
HinP1I	GCGC	2 cut(s)	714, 795
HincII	GTYRAC	3 cut(s)	214, 369, 388
HindII	GTYRAC	3 cut(s)	214, 369, 388
HinfI	GANTC	2 cut(s)	18, 135
HpaI	GTTAAC	1 cut(s)	388
HpaII	CCGG	2 cut(s)	111, 345
HphI	GGTGA	8 cut(s)	16, 79, 85, 158, 484, 571, 703, 745
Hpy166II	GTNNAC	6 cut(s)	87, 149, 214, 369, 388, 694
Hpy188I	TCNGA	2 cut(s)	381, 502
Hpy188III	TCNNGA	4 cut(s)	139, 196, 235, 762
Hpy8I	GTNNAC	6 cut(s)	87, 149, 214, 369, 388, 694
HpyAV	CCTTC	2 cut(s)	136, 676
HpyCH4III	ACNGT	4 cut(s)	91, 153, 568, 679
HpyCH4IV	ACGT	1 cut(s)	204
HpyCH4V	TGCA	4 cut(s)	119, 260, 479, 659
HpyF10VI	GCNNNNNNNGC	4 cut(s)	596, 621, 711, 733
HpyF3I	CTNAG	2 cut(s)	174, 760
HpySE526I	ACGT	1 cut(s)	204
Hsp92I	GRCGYC	1 cut(s)	736
Hsp92II	CATG	2 cut(s)	338, 659
HspAI	GCGC	2 cut(s)	714, 795
KspAI	GTTAAC	1 cut(s)	388
Kzo9I	GATC	4 cut(s)	49, 305, 433, 801
LguI	GCTCTTC	1 cut(s)	785
LmnI	GCTCC	3 cut(s)	319, 327, 601
MabI	ACCWGGT	1 cut(s)	262
MaeI	CTAG	3 cut(s)	357, 809, 829
MaeII	ACGT	1 cut(s)	204
MaeIII	GTNAC	1 cut(s)	91
MalI	GATC	4 cut(s)	51, 307, 435, 803
MbiI	CCGCTC	1 cut(s)	577
MboI	GATC	4 cut(s)	49, 305, 433, 801
MboII	GAAGA	6 cut(s)	44, 193, 221, 407, 742, 802
MfeI	CAATTG	1 cut(s)	642
MflI	RGATCY	2 cut(s)	49, 305
MhlI	GDGCHC	3 cut(s)	14, 173, 324
MluCI	AATT	2 cut(s)	487, 642
MlyI	GAGTC	1 cut(s)	12
MnlI	CCTC	5 cut(s)	463, 515, 647, 760, 830
MseI	TTAA	1 cut(s)	387
MslI	CAYNNNNRTG	1 cut(s)	339
MspA1I	CMGCKG	1 cut(s)	178
MspI	CCGG	2 cut(s)	111, 345
MspR9I	CCNGG	4 cut(s)	15, 264, 346, 778
MunI	CAATTG	1 cut(s)	642
Mva1269I	GAATGC	1 cut(s)	481
MvaI	CCWGG	3 cut(s)	15, 264, 778
MwoI	GCNNNNNNNGC	4 cut(s)	596, 621, 711, 733
NciI	CCSGG	1 cut(s)	346
NdeII	GATC	4 cut(s)	49, 305, 433, 801
NlaIII	CATG	2 cut(s)	338, 659
NlaIV	GGNNCC	1 cut(s)	12
NmuCI	GTSAC	1 cut(s)	91
NspI	RCATGY	1 cut(s)	659
PciSI	GCTCTTC	1 cut(s)	785
PctI	GAATGC	1 cut(s)	481
PfeI	GAWTC	1 cut(s)	135
PleI	GAGTC	1 cut(s)	12
PpsI	GAGTC	1 cut(s)	12
Psp124BI	GAGCTC	1 cut(s)	324
Psp6I	CCWGG	3 cut(s)	13, 262, 776
PspGI	CCWGG	3 cut(s)	13, 262, 776
PspN4I	GGNNCC	1 cut(s)	12
PspOMI	GGGCCC	1 cut(s)	10
PspPI	GGNCC	3 cut(s)	10, 11, 817
PsuI	RGATCY	2 cut(s)	49, 305
PvuII	CAGCTG	1 cut(s)	178
RseI	CAYNNNNRTG	1 cut(s)	339
SacI	GAGCTC	1 cut(s)	324
SapI	GCTCTTC	1 cut(s)	785
SaqAI	TTAA	1 cut(s)	387
Sau3AI	GATC	4 cut(s)	49, 305, 433, 801
Sau96I	GGNCC	3 cut(s)	10, 11, 817
SchI	GAGTC	1 cut(s)	12
ScrFI	CCNGG	4 cut(s)	15, 264, 346, 778
SduI	GDGCHC	3 cut(s)	14, 173, 324
SexAI	ACCWGGT	1 cut(s)	262
SmiMI	CAYNNNNRTG	1 cut(s)	339
SmlI	CTYRAG	2 cut(s)	269, 821
SmoI	CTYRAG	2 cut(s)	269, 821
Sse9I	AATT	2 cut(s)	487, 642
SsiI	CCGC	2 cut(s)	575, 599
SspMI	CTAG	3 cut(s)	357, 809, 829
SstI	GAGCTC	1 cut(s)	324
StyD4I	CCNGG	4 cut(s)	13, 262, 344, 776
StyI	CCWWGG	1 cut(s)	813
TaaI	ACNGT	4 cut(s)	91, 153, 568, 679
TaiI	ACGT	1 cut(s)	207
TaqI	TCGA	2 cut(s)	27, 444
TaqII	GACCGA	1 cut(s)	794
TasI	AATT	2 cut(s)	487, 642
TfiI	GAWTC	1 cut(s)	135
Tru1I	TTAA	1 cut(s)	387
Tru9I	TTAA	1 cut(s)	387
TscAI	CASTG	4 cut(s)	454, 571, 592, 684
TseFI	GTSAC	1 cut(s)	91
Tsp45I	GTSAC	1 cut(s)	91
TspDTI	ATGAA	2 cut(s)	267, 642
TspRI	CASTG	4 cut(s)	454, 571, 592, 684
XapI	RAATTY	1 cut(s)	487
XceI	RCATGY	1 cut(s)	659
XspI	CTAG	3 cut(s)	357, 809, 829

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.