pycom17g04220

Mitochondrial outer membrane protein porin of 34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003226
Physical Location & Seq
Reverse (-)
2897845 .. 2899965
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g04220.5

Sequence Viewer

Length: 828 bp
ATGGTGGGACCTGGGCTTTACACCGACATCGGCAAGAAAGCTAGAGATCTCCTGTACAAGGATTACCAGAGCGACAAGAAGTTCACCATCACTACCTTCTCTCCCACTGGAGTGGCTATCACTACTTCAGCAACCAACAAGGGTGAGCTTTTTCTGGGTGATGTGAACACTCAGTTGAAAAACAAGAATGTCACAACTGATATCAAAGTTGACACCGACTCCAATCTGTCCACCACTATTACGGTTGATCGATCTGCTCCTGGCCTGAAGGCAATCTTTAGCTTCAAAGTTCCTGATCAGAGGTCTGGCAAGGTGGAACTCCAATATCTGCATGACTTTGCGGGGATAAGCTCCAGTGTTGGTTTGACAGCCAACCCTATTGTTAACTTCTCCGGTGTTATTGGAACCAATCAGCTTGCACTTGGTACTGATCTGTCATTTGACACCAAGACTGGGATTTTGACTAAATGCAATGCTGGATTGAGCTTCTGCAATGCTGATCTAATTGCTGCATTGACCCTGAATGATAAAGGTGACATCCTCAGTGCATCGTACTATCACAGTGTCAATCCCCTCTCCAACACAGCTGTTGGTGCCGAGGTGACTCACAGGTTTCCCACCAACGAGAATACCATCACTCTTGGCACCCAGCACGCCCTTGATCCATTGACAACCATTAAGGCACGTGTGAACAACTTTGGCAAGGCAAGTGCCCTGGTCCAGCACGAGTGGCGCCCAAAGTCATTTGTCACTGTTTCAGGAGAGGTAGACACCAAGGCCATTGAGAAGAGCGCCAAGATTGGGTTGGCTCTCGCTCTCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005253 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005773 GO:0005774 GO:0005886 GO:0006091 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008308 GO:0008509 GO:0009060 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010029 GO:0010033 GO:0015075 GO:0015103 GO:0015267 GO:0015318 GO:0015698 GO:0015980 GO:0016020 GO:0016021 GO:0019867 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0030312 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031966 GO:0031967 GO:0031968 GO:0031974 GO:0031975 GO:0031981 GO:0032592 GO:0034220 GO:0042170 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045333 GO:0048580 GO:0050789 GO:0050793 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0055085 GO:0055114 GO:0065007 GO:0070013 GO:0071944 GO:0098542 GO:0098573 GO:0098588 GO:0098656 GO:0098805 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Porin_3

Protein Analysis

276

Amino Acids

29.36

Weight (kDa)

8.71

Isoelectric Point (pI)

7.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 593, 644, 732
AccI GTMKAC 1 cut(s) 768
AciI CCGC 1 cut(s) 341
AclWI GGATC 1 cut(s) 656
AcuI CTGAAG 2 cut(s) 111, 287
AcvI CACGTG 1 cut(s) 686
AcyI GRCGYC 1 cut(s) 733
AfaI GTAC 3 cut(s) 56, 427, 554
AfiI CCNNNNNNNGG 3 cut(s) 58, 453, 801
AflIII ACRYGT 1 cut(s) 685
AgsI TTSAA 2 cut(s) 178, 286
AjnI CCWGG 3 cut(s) 10, 259, 714
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 7 cut(s) 41, 148, 282, 351, 415, 486, 587
AluI AGCT 7 cut(s) 41, 148, 282, 351, 415, 486, 587
AlwI GGATC 1 cut(s) 656
AoxI GGCC 2 cut(s) 262, 777
ApeKI GCWGC 1 cut(s) 509
ArsI GACNNNNNNTTYG 2 cut(s) 442, 474
AspLEI GCGC 2 cut(s) 735, 794
AspS9I GGNCC 2 cut(s) 8, 718
AsuHPI GGTGA 5 cut(s) 76, 155, 170, 545, 613
AvaII GGWCC 2 cut(s) 8, 718
BaeGI GKGCMC 1 cut(s) 715
BanI GGYRCC 3 cut(s) 593, 644, 732
BauI CACGAG 1 cut(s) 725
BbrPI CACGTG 1 cut(s) 686
BbvI GCAGC 1 cut(s) 496
BccI CCATC 2 cut(s) 95, 641
BciT130I CCWGG 3 cut(s) 12, 261, 716
BclI TGATCA 1 cut(s) 295
BfaI CTAG 1 cut(s) 42
BfoI RGCGCY 2 cut(s) 736, 795
BglII AGATCT 1 cut(s) 46
BisI GCNGC 1 cut(s) 510
BlsI GCNGC 1 cut(s) 511
Bme1390I CCNGG 3 cut(s) 12, 261, 716
Bme18I GGWCC 2 cut(s) 8, 718
BmgT120I GGNCC 2 cut(s) 8, 718
BmiI GGNNCC 5 cut(s) 9, 406, 595, 646, 734
BmrFI CCNGG 3 cut(s) 12, 261, 716
BmrI ACTGGG 1 cut(s) 462
BmsI GCATC 1 cut(s) 557
BmuI ACTGGG 1 cut(s) 462
BpmI CTGGAG 2 cut(s) 129, 337
BpuEI CTTGAG 1 cut(s) 803
Bsa29I ATCGAT 1 cut(s) 250
BsaAI YACGTR 1 cut(s) 686
BsaHI GRCGYC 1 cut(s) 733
BsaJI CCNNGG 4 cut(s) 11, 597, 714, 774
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 203, 233
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 453, 801
Bse1I ACTGG 3 cut(s) 112, 354, 457
Bse3DI GCAATG 2 cut(s) 478, 499
BseBI CCWGG 3 cut(s) 12, 261, 716
BseCI ATCGAT 1 cut(s) 250
BseDI CCNNGG 4 cut(s) 11, 597, 714, 774
BseGI GGATG 1 cut(s) 537
BseLI CCNNNNNNNGG 3 cut(s) 58, 453, 801
BseMI GCAATG 2 cut(s) 478, 499
BseMII CTCAG 2 cut(s) 185, 556
BseNI ACTGG 3 cut(s) 112, 354, 457
BseSI GKGCMC 1 cut(s) 715
BseXI GCAGC 1 cut(s) 496
BseYI CCCAGC 1 cut(s) 648
BshFI GGCC 2 cut(s) 264, 779
BshNI GGYRCC 3 cut(s) 593, 644, 732
BshVI ATCGAT 1 cut(s) 250
BsiSI CCGG 1 cut(s) 393
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 3 cut(s) 58, 453, 801
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 2 cut(s) 264, 779
Bsp1286I GDGCHC 1 cut(s) 715
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 7 cut(s) 46, 247, 251, 295, 430, 499, 661
BspACI CCGC 1 cut(s) 341
BspANI GGCC 2 cut(s) 264, 779
BspCNI CTCAG 2 cut(s) 184, 555
BspDI ATCGAT 1 cut(s) 250
BspLI GGNNCC 5 cut(s) 9, 406, 595, 646, 734
BspPI GGATC 1 cut(s) 656
BspQI GCTCTTC 1 cut(s) 782
BspT107I GGYRCC 3 cut(s) 593, 644, 732
BsrDI GCAATG 2 cut(s) 478, 499
BsrGI TGTACA 1 cut(s) 54
BsrI ACTGG 3 cut(s) 112, 354, 457
BssECI CCNNGG 4 cut(s) 11, 597, 714, 774
BssMI GATC 7 cut(s) 46, 247, 251, 295, 430, 499, 661
BssNI GRCGYC 1 cut(s) 733
BssSI CACGAG 1 cut(s) 725
BssT1I CCWWGG 1 cut(s) 774
Bst2BI CACGAG 1 cut(s) 725
Bst2UI CCWGG 3 cut(s) 12, 261, 716
Bst4CI ACNGT 3 cut(s) 244, 563, 754
Bst6I CTCTTC 1 cut(s) 782
BstACI GRCGYC 1 cut(s) 733
BstAUI TGTACA 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 686
BstC8I GCNNGC 2 cut(s) 417, 654
BstDEI CTNAG 2 cut(s) 171, 542
BstENI CCTNNNNNAGG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 537
BstH2I RGCGCY 2 cut(s) 736, 795
BstHHI GCGC 2 cut(s) 735, 794
BstKTI GATC 7 cut(s) 49, 250, 254, 298, 433, 502, 664
BstMBI GATC 7 cut(s) 46, 247, 251, 295, 430, 499, 661
BstMWI GCNNNNNNNGC 2 cut(s) 593, 730
BstNI CCWGG 3 cut(s) 12, 261, 716
BstSCI CCNGG 3 cut(s) 10, 259, 714
BstSLI GKGCMC 1 cut(s) 715
BstV1I GCAGC 1 cut(s) 496
BstX2I RGATCY 1 cut(s) 46
BstXI CCANNNNNNTGG 1 cut(s) 112
BstYI RGATCY 1 cut(s) 46
Bsu15I ATCGAT 1 cut(s) 250
BsuRI GGCC 2 cut(s) 264, 779
BsuTUI ATCGAT 1 cut(s) 250
BtsCI GGATG 1 cut(s) 537
BtsIMutI CAGTG 5 cut(s) 105, 361, 550, 568, 750
Cac8I GCNNGC 2 cut(s) 417, 654
CfoI GCGC 2 cut(s) 735, 794
Cfr13I GGNCC 2 cut(s) 8, 718
ClaI ATCGAT 1 cut(s) 250
Csp6I GTAC 3 cut(s) 55, 426, 553
CviAII CATG 2 cut(s) 332, 825
CviQI GTAC 3 cut(s) 55, 426, 553
DdeI CTNAG 2 cut(s) 171, 542
DinI GGCGCC 1 cut(s) 734
DpnI GATC 7 cut(s) 48, 249, 253, 297, 432, 501, 663
DpnII GATC 7 cut(s) 46, 247, 251, 295, 430, 499, 661
Eam1104I CTCTTC 1 cut(s) 782
EarI CTCTTC 1 cut(s) 782
Eco130I CCWWGG 1 cut(s) 774
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 8, 718
Eco57I CTGAAG 2 cut(s) 111, 287
Eco72I CACGTG 1 cut(s) 686
EcoNI CCTNNNNNAGG 1 cut(s) 56
EcoO109I RGGNCCY 1 cut(s) 8
EcoRII CCWGG 3 cut(s) 10, 259, 714
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 774
EgeI GGCGCC 1 cut(s) 734
EheI GGCGCC 1 cut(s) 734
ErhI CCWWGG 1 cut(s) 774
FaeI CATG 2 cut(s) 335, 828
FaiI YATR 2 cut(s) 333, 826
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FaqI GGGAC 1 cut(s) 21
FatI CATG 2 cut(s) 331, 824
FauI CCCGC 1 cut(s) 334
FbaI TGATCA 1 cut(s) 295
FblI GTMKAC 1 cut(s) 768
Fnu4HI GCNGC 1 cut(s) 510
FokI GGATG 1 cut(s) 524
Fsp4HI GCNGC 1 cut(s) 510
FspBI CTAG 1 cut(s) 42
GlaI GCGC 2 cut(s) 734, 793
GluI GCNGC 1 cut(s) 510
GsaI CCCAGC 1 cut(s) 652
GsuI CTGGAG 2 cut(s) 129, 337
HaeII RGCGCY 2 cut(s) 736, 795
HaeIII GGCC 2 cut(s) 264, 779
HapII CCGG 1 cut(s) 393
HhaI GCGC 2 cut(s) 735, 794
Hin1I GRCGYC 1 cut(s) 733
Hin1II CATG 2 cut(s) 335, 828
Hin6I GCGC 2 cut(s) 733, 792
HinP1I GCGC 2 cut(s) 733, 792
HincII GTYRAC 2 cut(s) 211, 385
HindII GTYRAC 2 cut(s) 211, 385
HinfI GANTC 2 cut(s) 218, 604
HpaI GTTAAC 1 cut(s) 385
HpaII CCGG 1 cut(s) 393
HphI GGTGA 5 cut(s) 76, 155, 170, 545, 613
Hpy166II GTNNAC 7 cut(s) 84, 166, 211, 231, 385, 691, 769
Hpy188I TCNGA 1 cut(s) 300
Hpy188III TCNNGA 2 cut(s) 293, 759
Hpy8I GTNNAC 7 cut(s) 84, 166, 211, 231, 385, 691, 769
HpyAV CCTTC 2 cut(s) 106, 262
HpyCH4III ACNGT 3 cut(s) 244, 563, 754
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 6 cut(s) 331, 419, 471, 492, 512, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 593, 730
HpyF3I CTNAG 2 cut(s) 171, 542
HpySE526I ACGT 1 cut(s) 685
Hsp92I GRCGYC 1 cut(s) 733
Hsp92II CATG 2 cut(s) 335, 828
HspAI GCGC 2 cut(s) 733, 792
KasI GGCGCC 1 cut(s) 732
Ksp22I TGATCA 1 cut(s) 295
KspAI GTTAAC 1 cut(s) 385
Kzo9I GATC 7 cut(s) 46, 247, 251, 295, 430, 499, 661
LguI GCTCTTC 1 cut(s) 782
LmnI GCTCC 2 cut(s) 262, 356
Lsp1109I GCAGC 1 cut(s) 496
LweI GCATC 1 cut(s) 557
MaeI CTAG 1 cut(s) 42
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 4 cut(s) 190, 533, 601, 748
MalI GATC 7 cut(s) 48, 249, 253, 297, 432, 501, 663
MboI GATC 7 cut(s) 46, 247, 251, 295, 430, 499, 661
MboII GAAGA 1 cut(s) 799
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 715
MluCI AATT 1 cut(s) 504
Mly113I GGCGCC 1 cut(s) 733
MlyI GAGTC 2 cut(s) 212, 598
MmeI TCCRAC 1 cut(s) 603
MnlI CCTC 5 cut(s) 294, 551, 584, 592, 757
MseI TTAA 2 cut(s) 384, 678
MslI CAYNNNNRTG 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 1 cut(s) 393
MspR9I CCNGG 3 cut(s) 12, 261, 716
MvaI CCWGG 3 cut(s) 12, 261, 716
MwoI GCNNNNNNNGC 2 cut(s) 593, 730
NarI GGCGCC 1 cut(s) 733
NdeII GATC 7 cut(s) 46, 247, 251, 295, 430, 499, 661
NlaIII CATG 2 cut(s) 335, 828
NlaIV GGNNCC 5 cut(s) 9, 406, 595, 646, 734
NmeAIII GCCGAG 1 cut(s) 622
NmuCI GTSAC 4 cut(s) 190, 533, 601, 748
OliI CACNNNNGTG 1 cut(s) 110
PciSI GCTCTTC 1 cut(s) 782
PkrI GCNGC 1 cut(s) 511
PleI GAGTC 2 cut(s) 212, 598
PluTI GGCGCC 1 cut(s) 736
PmaCI CACGTG 1 cut(s) 686
PmlI CACGTG 1 cut(s) 686
PpsI GAGTC 2 cut(s) 212, 598
Ppu21I YACGTR 1 cut(s) 686
PpuMI RGGWCCY 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 8
Psp6I CCWGG 3 cut(s) 10, 259, 714
PspCI CACGTG 1 cut(s) 686
PspFI CCCAGC 1 cut(s) 648
PspGI CCWGG 3 cut(s) 10, 259, 714
PspN4I GGNNCC 5 cut(s) 9, 406, 595, 646, 734
PspPI GGNCC 2 cut(s) 8, 718
PspPPI RGGWCCY 1 cut(s) 8
PsuI RGATCY 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 3 cut(s) 56, 427, 554
RsaNI GTAC 3 cut(s) 55, 426, 553
RseI CAYNNNNRTG 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 782
SaqAI TTAA 2 cut(s) 384, 678
SatI GCNGC 1 cut(s) 510
Sau3AI GATC 7 cut(s) 46, 247, 251, 295, 430, 499, 661
Sau96I GGNCC 2 cut(s) 8, 718
SchI GAGTC 2 cut(s) 212, 598
ScrFI CCNGG 3 cut(s) 12, 261, 716
SduI GDGCHC 1 cut(s) 715
SfaNI GCATC 1 cut(s) 557
SfoI GGCGCC 1 cut(s) 734
SinI GGWCC 2 cut(s) 8, 718
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 818
SmoI CTYRAG 1 cut(s) 818
Sse9I AATT 1 cut(s) 504
SsiI CCGC 1 cut(s) 341
SspDI GGCGCC 1 cut(s) 732
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 3 cut(s) 10, 259, 714
StyI CCWWGG 1 cut(s) 774
TaaI ACNGT 3 cut(s) 244, 563, 754
TaiI ACGT 1 cut(s) 688
TaqI TCGA 1 cut(s) 250
TasI AATT 1 cut(s) 504
TatI WGTACW 1 cut(s) 54
Tru1I TTAA 2 cut(s) 384, 678
Tru9I TTAA 2 cut(s) 384, 678
TscAI CASTG 5 cut(s) 112, 361, 550, 568, 757
TseFI GTSAC 4 cut(s) 190, 533, 601, 748
TseI GCWGC 1 cut(s) 509
Tsp45I GTSAC 4 cut(s) 190, 533, 601, 748
TspRI CASTG 5 cut(s) 112, 361, 550, 568, 757
VpaK11BI GGWCC 2 cut(s) 8, 718
XagI CCTNNNNNAGG 1 cut(s) 56
XcmI CCANNNNNNNNNTGG 1 cut(s) 802
XmiI GTMKAC 1 cut(s) 768
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.