AT3G07920

eukaryotic translation initiation factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003100
Physical Location & Seq
Reverse (-)
2524420 .. 2526229
1810 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G07920.1

Sequence Viewer

Length: 495 bp
ATGGCTGATGAAAGTAATGAGAACAAGAAAGAAGTGAAGGATGATGAGCAAGAAATTCTTCGTAGGGTCTTTAATATCCTGCGTGAAAATAGTCCGGAGCTTGTTGGAATTTGGCTTCTTACAATTATATGGCCACCACAAGTTCTTCGTGAAGAGACAGCGAAAGGCACAAAGAAGACAGTCTTTGTCAACTTTATGGACTATTGCAAGACGATGCATCGAAACCCTGATCATGTTATGGCGTTCTTGCTTGCTGAGCTGGGTACTAGGGGTAAACTTGATGATCAGCAAAGGTTGGTTGTTAGGGGGAGATTTACACAGAGGGACTTTGAATCGTTATTACGAGGATATATCCTTGACTACGTCATGTGCATTCCTTGCAAGAGCACAGAGGCAATTCTTTCAAAGGAGAATCGTCTCTTCTTTTTGAGATGTGGAAAGTGTGGATCAGGACGACCTGTGGCGCCAATCAAAATCAATTTTGGTCGCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

164

Amino Acids

19.11

Weight (kDa)

9.18

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-5_eIF-2B PF01873 54 - 150 3.7e-33 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 480
AccB1I GGYRCC 1 cut(s) 463
AccIII TCCGGA 1 cut(s) 94
AclWI GGATC 1 cut(s) 454
AcoI YGGCCR 1 cut(s) 131
AcsI RAATTY 2 cut(s) 54, 108
AcyI GRCGYC 1 cut(s) 464
AfaI GTAC 1 cut(s) 265
AgsI TTSAA 2 cut(s) 332, 405
AluBI AGCT 2 cut(s) 100, 259
AluI AGCT 2 cut(s) 100, 259
Alw21I GWGCWC 1 cut(s) 389
Alw26I GTCTC 2 cut(s) 149, 422
AlwI GGATC 1 cut(s) 454
Aor13HI TCCGGA 1 cut(s) 94
AoxI GGCC 1 cut(s) 131
ApoI RAATTY 2 cut(s) 54, 108
Asp700I GAANNNNTTC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 466
BalI TGGCCA 1 cut(s) 133
BanI GGYRCC 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 389
BclI TGATCA 2 cut(s) 229, 283
BcoDI GTCTC 2 cut(s) 149, 422
BfaI CTAG 1 cut(s) 267
BfoI RGCGCY 1 cut(s) 467
BfuAI ACCTGC 1 cut(s) 480
BlpI GCTNAGC 1 cut(s) 255
BmiI GGNNCC 1 cut(s) 465
BmsI GCATC 2 cut(s) 204, 226
BpiI GAAGAC 1 cut(s) 182
Bpu1102I GCTNAGC 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 464
BsaWI WCCGGW 1 cut(s) 94
BseAI TCCGGA 1 cut(s) 94
BseGI GGATG 1 cut(s) 46
BseMII CTCAG 1 cut(s) 246
BseYI CCCAGC 1 cut(s) 259
BshFI GGCC 1 cut(s) 133
BshNI GGYRCC 1 cut(s) 463
BsiHKAI GWGCWC 1 cut(s) 389
BsiSI CCGG 1 cut(s) 95
BslFI GGGAC 1 cut(s) 338
BsmAI GTCTC 2 cut(s) 149, 422
BsmBI CGTCTC 1 cut(s) 422
BsmFI GGGAC 1 cut(s) 338
BsmI GAATGC 1 cut(s) 372
BsnI GGCC 1 cut(s) 133
Bsp1286I GDGCHC 1 cut(s) 389
Bsp13I TCCGGA 1 cut(s) 94
Bsp143I GATC 3 cut(s) 229, 283, 446
Bsp1720I GCTNAGC 1 cut(s) 255
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 1 cut(s) 247
BspEI TCCGGA 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 465
BspMI ACCTGC 1 cut(s) 480
BspPI GGATC 1 cut(s) 454
BspT107I GGYRCC 1 cut(s) 463
BssMI GATC 3 cut(s) 229, 283, 446
BssNI GRCGYC 1 cut(s) 464
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 2 cut(s) 147, 425
BstACI GRCGYC 1 cut(s) 464
BstAPI GCANNNNNTGC 1 cut(s) 378
BstC8I GCNNGC 1 cut(s) 252
BstDEI CTNAG 1 cut(s) 255
BstF5I GGATG 1 cut(s) 46
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 3 cut(s) 232, 286, 449
BstMAI GTCTC 2 cut(s) 149, 422
BstMBI GATC 3 cut(s) 229, 283, 446
BstMWI GCNNNNNNNGC 2 cut(s) 256, 378
BstV2I GAAGAC 1 cut(s) 182
BsuRI GGCC 1 cut(s) 133
BtsCI GGATG 1 cut(s) 46
BveI ACCTGC 1 cut(s) 480
Cac8I GCNNGC 1 cut(s) 252
CfoI GCGC 1 cut(s) 466
Csp6I GTAC 1 cut(s) 264
CviAII CATG 2 cut(s) 233, 367
CviJI RGCY 5 cut(s) 5, 100, 115, 133, 259
CviKI_1 RGCY 5 cut(s) 5, 100, 115, 133, 259
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 1 cut(s) 255
DinI GGCGCC 1 cut(s) 465
DpnI GATC 3 cut(s) 231, 285, 448
DpnII GATC 3 cut(s) 229, 283, 446
EaeI YGGCCR 1 cut(s) 131
Eam1104I CTCTTC 2 cut(s) 147, 425
EarI CTCTTC 2 cut(s) 147, 425
EcoT22I ATGCAT 1 cut(s) 219
EgeI GGCGCC 1 cut(s) 465
EheI GGCGCC 1 cut(s) 465
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 2 cut(s) 236, 370
FaiI YATR 7 cut(s) 128, 130, 197, 234, 239, 351, 368
FalI AAGNNNNNCTT 4 cut(s) 42, 74, 167, 199
FaqI GGGAC 1 cut(s) 338
FatI CATG 2 cut(s) 232, 366
FbaI TGATCA 2 cut(s) 229, 283
FokI GGATG 1 cut(s) 53
FspBI CTAG 1 cut(s) 267
GlaI GCGC 1 cut(s) 465
GsaI CCCAGC 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 1 cut(s) 133
HapII CCGG 1 cut(s) 95
HhaI GCGC 1 cut(s) 466
Hin1I GRCGYC 1 cut(s) 464
Hin1II CATG 2 cut(s) 236, 370
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 2 cut(s) 332, 412
HpaII CCGG 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 190, 275
Hpy188III TCNNGA 3 cut(s) 95, 149, 450
Hpy8I GTNNAC 2 cut(s) 190, 275
HpyAV CCTTC 1 cut(s) 31
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 4 cut(s) 207, 217, 372, 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 378
HpyF3I CTNAG 1 cut(s) 255
HpySE526I ACGT 1 cut(s) 363
Hsp92I GRCGYC 1 cut(s) 464
Hsp92II CATG 2 cut(s) 236, 370
HspAI GCGC 1 cut(s) 464
KasI GGCGCC 1 cut(s) 463
Kpn2I TCCGGA 1 cut(s) 94
Ksp22I TGATCA 2 cut(s) 229, 283
Kzo9I GATC 3 cut(s) 229, 283, 446
LmnI GCTCC 1 cut(s) 97
LpnPI CCDG 7 cut(s) 92, 108, 240, 245, 435, 471, 475
LweI GCATC 2 cut(s) 204, 226
MaeI CTAG 1 cut(s) 267
MaeII ACGT 1 cut(s) 363
MalI GATC 3 cut(s) 231, 285, 448
MboI GATC 3 cut(s) 229, 283, 446
MboII GAAGA 5 cut(s) 50, 137, 164, 187, 412
MhlI GDGCHC 1 cut(s) 389
MlsI TGGCCA 1 cut(s) 133
MluCI AATT 5 cut(s) 54, 108, 123, 396, 478
MluNI TGGCCA 1 cut(s) 133
Mly113I GGCGCC 1 cut(s) 464
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 3 cut(s) 315, 338, 385
Mox20I TGGCCA 1 cut(s) 133
Mph1103I ATGCAT 1 cut(s) 219
MroI TCCGGA 1 cut(s) 94
MroXI GAANNNNTTC 1 cut(s) 57
MscI TGGCCA 1 cut(s) 133
MseI TTAA 1 cut(s) 72
Msp20I TGGCCA 1 cut(s) 133
MspI CCGG 1 cut(s) 95
Mva1269I GAATGC 1 cut(s) 372
MwoI GCNNNNNNNGC 2 cut(s) 256, 378
NarI GGCGCC 1 cut(s) 464
NdeII GATC 3 cut(s) 229, 283, 446
NlaIII CATG 2 cut(s) 236, 370
NlaIV GGNNCC 1 cut(s) 465
NsiI ATGCAT 1 cut(s) 219
PctI GAATGC 1 cut(s) 372
PdmI GAANNNNTTC 1 cut(s) 57
PfeI GAWTC 2 cut(s) 332, 412
PflFI GACNNNGTC 1 cut(s) 362
PluTI GGCGCC 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 259
PspN4I GGNNCC 1 cut(s) 465
PsyI GACNNNGTC 1 cut(s) 362
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SaqAI TTAA 1 cut(s) 72
Sau3AI GATC 3 cut(s) 229, 283, 446
SduI GDGCHC 1 cut(s) 389
SetI ASST 6 cut(s) 102, 261, 296, 366, 460, 494
SfaNI GCATC 2 cut(s) 204, 226
SfoI GGCGCC 1 cut(s) 465
Sse9I AATT 5 cut(s) 54, 108, 123, 396, 478
SspDI GGCGCC 1 cut(s) 463
SspMI CTAG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 1 cut(s) 366
TaqI TCGA 1 cut(s) 220
TasI AATT 5 cut(s) 54, 108, 123, 396, 478
TfiI GAWTC 2 cut(s) 332, 412
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TspDTI ATGAA 1 cut(s) 24
Tth111I GACNNNGTC 1 cut(s) 362
XapI RAATTY 2 cut(s) 54, 108
XmnI GAANNNNTTC 1 cut(s) 57
XspI CTAG 1 cut(s) 267
Zsp2I ATGCAT 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.