Rorug01G0415500

MINDY deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003100
Physical Location & Seq
Forward (+)
51883821 .. 51885814
1994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0415500.1

Sequence Viewer

Length: 1422 bp
ATGGATGAAAGGCTGATTGAGGCTTCTCGAAGTGGAAATGTCCAACTTTTGAATCAGTTGCTGGCAGAAAATACAGACATACTCCATAGTGTTGCGCTGGCTTCGGGAGAGAACCCTTTACACATTGCTTCCTTGGCTGGCCATGTTGAGTTTGTTAAGGAGGTTCTAAGGTTGAAACCAGCTTTTGCTAAAGAAATCAACCAGGATTGTTATAGCCCGATGCATATTGCGTCTGCCAACGGGCATTTGGAGATTGTAAAGGAGCTGCTTCAAGTTGATCCAAGACTCTCCCAGCTTAAAGGAAGAGATCAATGTACAGCTCTTCATTATGCAGCTCGCAGAGGGAGGATAGAAATTATTAAAGAAATGGTTTCGGCTTGTTCAGAATGTGTCGAAGATGTGACCGAGCAAGGGGAGACTGTTTTTCACCTTGCTGTCAAGAACAACCAGTTTGAAGCTATTGATGCTATGGTGAAATTGGTCCGTGAAAGGAACAAACTTGACATCCTGAATATGAAAGATAATAATGGCAACTCAGTTCTTCACCTAGCAACCTGGAAGAAGCAACACCAGGTGGTGCAATGTTTACTTGCCATTAACGGAACTAGTACTCCGGGTACATTGGACGTAAACAGTGTGAATCAGAGTGGTCTAACAGCTCTTGATCTGCTACTAATCTTCCCTAGTGGAGCCGGTGACCTGGAGATCGATGGTATCCTTCGCGGTGCAGGAGCTTTGAGAGCACGAGACATAGTCCATAGTAATCACAACCATACCGTATTAAGTGTCCCCGAAGCATCAGAGAGCCATGATAGTTTGCTCATGGACCCAAAAAATTTGGTGGAGTACTTCAAGTTCAAAAGGGGCAGAGACTCCCCTGATGACGCACGAACCGCTCTGTTAGTCGTGGCAGTACTGGTGGCTACGGCAACCTATGACACTGCAATAAACCCTCCAGGTGGTCTTTGGCAGGACACAAACTTAAGCAAGAATGGGACTGATCCACCACACCTTGTCGGGACGGCGAATATCGCCTCTTATAACACAGCTTACTACGTCGTTGGGGCAGTTTTCAACTCCATAGGGTTTTCAGTTTCTCTTCACATGATCAATATATATCCTCACAACCAATTTCCCTTTGCGGTTGGAGCTTCAAGTTTGTGGTGTTGCAATGTACTTTACGTACGTCACTGCATTGATTACTATATCTCCAGCTTTGACCTCAGGGTGGTACACATTGTTTGTACTTGTGCCATTTCTGCCTATGGTGGTACTACTTGCCGTGAGGTTTATCATCAAGCTTACAGAACCTCTGCGAAATCGACTGCAGACCTGCGGATTTGTACTAAATTAATTTACAGTCCTAATTCGATGTGTATATGCATGTCATTTGAACATGATTCATCGAAGGAAAGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MINDY_DUB

Protein Analysis

473

Amino Acids

52.1

Weight (kDa)

6.2

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 91 9.6e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 74 - 121 2.7e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 100 - 158 4.5e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 108 - 198 1.3e-11 Ankyrin repeats (3 copies)
PGG PF13962 295 - 374 1.5e-12 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1041
Acc36I ACCTGC 1 cut(s) 1341
AccBSI CCGCTC 1 cut(s) 896
AccII CGCG 1 cut(s) 723
AciI CCGC 4 cut(s) 723, 894, 1142, 1336
AclWI GGATC 2 cut(s) 272, 995
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 1 cut(s) 835
AdeI CACNNNGTG 1 cut(s) 574
AfiI CCNNNNNNNGG 3 cut(s) 411, 959, 1228
AflII CTTAAG 1 cut(s) 982
AgsI TTSAA 9 cut(s) 52, 175, 272, 455, 853, 859, 1075, 1155, 1394
AhlI ACTAGT 1 cut(s) 605
AjnI CCWGG 5 cut(s) 201, 554, 570, 699, 955
AloI GAACNNNNNNTCC 2 cut(s) 595, 627
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 3 cut(s) 410, 741, 864
AlwI GGATC 2 cut(s) 272, 995
AlwNI CAGNNNCTG 1 cut(s) 61
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 265, 332
ApoI RAATTY 1 cut(s) 835
AseI ATTAAT 1 cut(s) 1352
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 2 cut(s) 481, 826
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 4 cut(s) 419, 484, 536, 707
AvaII GGWCC 2 cut(s) 481, 826
AxyI CCTNAGG 1 cut(s) 1223
BalI TGGCCA 1 cut(s) 141
BauI CACGAG 1 cut(s) 744
Bbv12I GWGCWC 1 cut(s) 745
BbvI GCAGC 2 cut(s) 252, 344
BccI CCATC 1 cut(s) 704
BceAI ACGGC 3 cut(s) 942, 1038, 1266
BciT130I CCWGG 5 cut(s) 203, 556, 572, 701, 957
BciVI GTATCC 1 cut(s) 725
BclI TGATCA 1 cut(s) 1107
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 3 cut(s) 410, 741, 864
BcuI ACTAGT 1 cut(s) 605
BfaI CTAG 3 cut(s) 548, 606, 684
BfmI CTRYAG 1 cut(s) 1326
BfrI CTTAAG 1 cut(s) 982
BfuAI ACCTGC 1 cut(s) 1341
BfuI GTATCC 1 cut(s) 725
BisI GCNGC 2 cut(s) 266, 333
BlsI GCNGC 2 cut(s) 267, 334
BmcAI AGTACT 3 cut(s) 610, 848, 915
Bme1390I CCNGG 6 cut(s) 203, 556, 572, 615, 701, 957
Bme18I GGWCC 2 cut(s) 481, 826
BmgT120I GGNCC 2 cut(s) 481, 826
BmiI GGNNCC 2 cut(s) 691, 828
BmrFI CCNGG 6 cut(s) 203, 556, 572, 615, 701, 957
BmsI GCATC 3 cut(s) 210, 454, 806
BpmI CTGGAG 3 cut(s) 722, 939, 1195
BpuMI CCSGG 1 cut(s) 615
Bsa29I ATCGAT 1 cut(s) 708
BsaAI YACGTR 1 cut(s) 1183
BsaJI CCNNGG 1 cut(s) 132
BsaXI ACNNNNNCTCC 4 cut(s) 595, 625, 1193, 1223
Bsc4I CCNNNNNNNGG 3 cut(s) 411, 959, 1228
Bse118I RCCGGY 1 cut(s) 692
Bse1I ACTGG 2 cut(s) 448, 921
Bse21I CCTNAGG 1 cut(s) 1223
Bse3DI GCAATG 3 cut(s) 123, 587, 1177
BseBI CCWGG 5 cut(s) 203, 556, 572, 701, 957
BseCI ATCGAT 1 cut(s) 708
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 2 cut(s) 10, 504
BseLI CCNNNNNNNGG 3 cut(s) 411, 959, 1228
BseMI GCAATG 3 cut(s) 123, 587, 1177
BseMII CTCAG 2 cut(s) 549, 1237
BseNI ACTGG 2 cut(s) 448, 921
BseXI GCAGC 2 cut(s) 252, 344
BseYI CCCAGC 1 cut(s) 291
BsgI GTGCAG 1 cut(s) 747
Bsh1236I CGCG 1 cut(s) 723
BshFI GGCC 1 cut(s) 141
BshVI ATCGAT 1 cut(s) 708
BsiHKAI GWGCWC 1 cut(s) 745
BsiSI CCGG 2 cut(s) 614, 693
BsiWI CGTACG 1 cut(s) 1183
BslFI GGGAC 3 cut(s) 773, 1009, 1033
BslI CCNNNNNNNGG 3 cut(s) 411, 959, 1228
BsmAI GTCTC 3 cut(s) 410, 741, 864
BsmFI GGGAC 3 cut(s) 773, 1009, 1033
BsnI GGCC 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 745
Bsp1407I TGTACA 1 cut(s) 314
Bsp143I GATC 6 cut(s) 277, 307, 664, 705, 1000, 1107
BspACI CCGC 4 cut(s) 723, 894, 1142, 1336
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 2 cut(s) 548, 1236
BspDI ATCGAT 1 cut(s) 708
BspFNI CGCG 1 cut(s) 723
BspLI GGNNCC 2 cut(s) 691, 828
BspMAI CTGCAG 1 cut(s) 1330
BspMI ACCTGC 1 cut(s) 1341
BspPI GGATC 2 cut(s) 272, 995
BspQI GCTCTTC 1 cut(s) 327
BspTI CTTAAG 1 cut(s) 982
BsrBI CCGCTC 1 cut(s) 896
BsrDI GCAATG 3 cut(s) 123, 587, 1177
BsrFI RCCGGY 1 cut(s) 692
BsrGI TGTACA 1 cut(s) 314
BsrI ACTGG 2 cut(s) 448, 921
BssAI RCCGGY 1 cut(s) 692
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 6 cut(s) 277, 307, 664, 705, 1000, 1107
BssSI CACGAG 1 cut(s) 744
BssT1I CCWWGG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 744
Bst2UI CCWGG 5 cut(s) 203, 556, 572, 701, 957
Bst4CI ACNGT 4 cut(s) 421, 635, 778, 1361
Bst6I CTCTTC 3 cut(s) 298, 327, 1104
BstAFI CTTAAG 1 cut(s) 982
BstAUI TGTACA 1 cut(s) 314
BstBAI YACGTR 1 cut(s) 1183
BstC8I GCNNGC 4 cut(s) 63, 99, 139, 337
BstDEI CTNAG 3 cut(s) 167, 535, 1223
BstEII GGTNACC 1 cut(s) 695
BstF5I GGATG 2 cut(s) 10, 504
BstFNI CGCG 1 cut(s) 723
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 6 cut(s) 280, 310, 667, 708, 1003, 1110
BstMAI GTCTC 3 cut(s) 410, 741, 864
BstMBI GATC 6 cut(s) 277, 307, 664, 705, 1000, 1107
BstMWI GCNNNNNNNGC 7 cut(s) 134, 464, 740, 893, 1031, 1148, 1259
BstNI CCWGG 5 cut(s) 203, 556, 572, 701, 957
BstNSI RCATGY 1 cut(s) 1387
BstPI GGTNACC 1 cut(s) 695
BstSCI CCNGG 6 cut(s) 201, 554, 570, 613, 699, 955
BstSFI CTRYAG 1 cut(s) 1326
BstSNI TACGTA 1 cut(s) 1183
BstUI CGCG 1 cut(s) 723
BstV1I GCAGC 2 cut(s) 252, 344
Bsu15I ATCGAT 1 cut(s) 708
Bsu36I CCTNAGG 1 cut(s) 1223
BsuI GTATCC 1 cut(s) 725
BsuRI GGCC 1 cut(s) 141
BsuTUI ATCGAT 1 cut(s) 708
BtsCI GGATG 2 cut(s) 10, 504
BtsI GCAGTG 2 cut(s) 939, 1189
BtsIMutI CAGTG 3 cut(s) 640, 939, 1189
BveI ACCTGC 1 cut(s) 1341
Cac8I GCNNGC 4 cut(s) 63, 99, 139, 337
CaiI CAGNNNCTG 1 cut(s) 61
CfoI GCGC 1 cut(s) 97
Cfr10I RCCGGY 1 cut(s) 692
Cfr13I GGNCC 2 cut(s) 481, 826
ClaI ATCGAT 1 cut(s) 708
CseI GACGC 2 cut(s) 219, 893
CsiI ACCWGGT 1 cut(s) 570
CviAII CATG 6 cut(s) 143, 809, 823, 1105, 1384, 1397
DdeI CTNAG 3 cut(s) 167, 535, 1223
DpnI GATC 6 cut(s) 279, 309, 666, 707, 1002, 1109
DpnII GATC 6 cut(s) 277, 307, 664, 705, 1000, 1107
DraIII CACNNNGTG 1 cut(s) 574
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 3 cut(s) 298, 327, 1104
EarI CTCTTC 3 cut(s) 298, 327, 1104
Eco105I TACGTA 1 cut(s) 1183
Eco130I CCWWGG 1 cut(s) 132
Eco47I GGWCC 2 cut(s) 481, 826
Eco81I CCTNAGG 1 cut(s) 1223
Eco91I GGTNACC 1 cut(s) 695
EcoO65I GGTNACC 1 cut(s) 695
EcoRII CCWGG 5 cut(s) 201, 554, 570, 699, 955
EcoT14I CCWWGG 1 cut(s) 132
EcoT22I ATGCAT 2 cut(s) 225, 1385
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 6 cut(s) 146, 812, 826, 1108, 1387, 1400
FaqI GGGAC 3 cut(s) 773, 1009, 1033
FatI CATG 6 cut(s) 142, 808, 822, 1104, 1383, 1396
FbaI TGATCA 1 cut(s) 1107
Fnu4HI GCNGC 2 cut(s) 266, 333
FokI GGATG 2 cut(s) 17, 491
Fsp4HI GCNGC 2 cut(s) 266, 333
FspBI CTAG 3 cut(s) 548, 606, 684
GlaI GCGC 1 cut(s) 96
GluI GCNGC 2 cut(s) 266, 333
GsaI CCCAGC 1 cut(s) 295
GsuI CTGGAG 3 cut(s) 722, 939, 1195
HaeIII GGCC 1 cut(s) 141
HapII CCGG 2 cut(s) 614, 693
HgaI GACGC 2 cut(s) 219, 893
HhaI GCGC 1 cut(s) 97
Hin1II CATG 6 cut(s) 146, 812, 826, 1108, 1387, 1400
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HindIII AAGCTT 1 cut(s) 1299
HinfI GANTC 5 cut(s) 52, 285, 640, 872, 1400
HpaII CCGG 2 cut(s) 614, 693
HphI GGTGA 4 cut(s) 419, 484, 536, 707
Hpy166II GTNNAC 3 cut(s) 587, 631, 1234
Hpy188I TCNGA 3 cut(s) 385, 645, 802
Hpy188III TCNNGA 6 cut(s) 27, 105, 439, 508, 662, 1018
Hpy8I GTNNAC 3 cut(s) 587, 631, 1234
Hpy99I CGWCG 1 cut(s) 1061
HpyAV CCTTC 2 cut(s) 728, 1402
HpyCH4III ACNGT 4 cut(s) 421, 635, 778, 1361
HpyCH4IV ACGT 4 cut(s) 627, 1056, 1182, 1186
HpyCH4V TGCA 9 cut(s) 223, 332, 580, 728, 944, 1170, 1194, 1328, 1383
HpyF10VI GCNNNNNNNGC 7 cut(s) 134, 464, 740, 893, 1031, 1148, 1259
HpyF3I CTNAG 3 cut(s) 167, 535, 1223
HpySE526I ACGT 4 cut(s) 627, 1056, 1182, 1186
Hsp92II CATG 6 cut(s) 146, 812, 826, 1108, 1387, 1400
HspAI GCGC 1 cut(s) 95
Ksp22I TGATCA 1 cut(s) 1107
Kzo9I GATC 6 cut(s) 277, 307, 664, 705, 1000, 1107
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 4 cut(s) 262, 689, 731, 1148
Lsp1109I GCAGC 2 cut(s) 252, 344
LweI GCATC 3 cut(s) 210, 454, 806
MabI ACCWGGT 1 cut(s) 570
MaeI CTAG 3 cut(s) 548, 606, 684
MaeII ACGT 4 cut(s) 627, 1056, 1182, 1186
MaeIII GTNAC 3 cut(s) 400, 695, 1187
MalI GATC 6 cut(s) 279, 309, 666, 707, 1002, 1109
MbiI CCGCTC 1 cut(s) 896
MboI GATC 6 cut(s) 277, 307, 664, 705, 1000, 1107
MboII GAAGA 7 cut(s) 314, 315, 407, 533, 571, 670, 1091
MhlI GDGCHC 1 cut(s) 745
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 7 cut(s) 354, 476, 835, 1130, 1349, 1353, 1366
MluNI TGGCCA 1 cut(s) 141
MlyI GAGTC 2 cut(s) 279, 866
MmeI TCCRAC 2 cut(s) 67, 1126
Mox20I TGGCCA 1 cut(s) 141
Mph1103I ATGCAT 2 cut(s) 225, 1385
MscI TGGCCA 1 cut(s) 141
MseI TTAA 7 cut(s) 156, 297, 360, 597, 782, 983, 1352
Msp20I TGGCCA 1 cut(s) 141
MspCI CTTAAG 1 cut(s) 982
MspI CCGG 2 cut(s) 614, 693
MspR9I CCNGG 6 cut(s) 203, 556, 572, 615, 701, 957
MvaI CCWGG 5 cut(s) 203, 556, 572, 701, 957
MvnI CGCG 1 cut(s) 723
MwoI GCNNNNNNNGC 7 cut(s) 134, 464, 740, 893, 1031, 1148, 1259
NciI CCSGG 1 cut(s) 615
NdeII GATC 6 cut(s) 277, 307, 664, 705, 1000, 1107
NlaIII CATG 6 cut(s) 146, 812, 826, 1108, 1387, 1400
NlaIV GGNNCC 2 cut(s) 691, 828
NmuCI GTSAC 3 cut(s) 400, 695, 1187
NsiI ATGCAT 2 cut(s) 225, 1385
NspI RCATGY 1 cut(s) 1387
PciSI GCTCTTC 1 cut(s) 327
PfeI GAWTC 3 cut(s) 52, 640, 1400
Pfl23II CGTACG 1 cut(s) 1183
PflFI GACNNNGTC 1 cut(s) 752
PkrI GCNGC 2 cut(s) 267, 334
PleI GAGTC 2 cut(s) 279, 866
PpsI GAGTC 2 cut(s) 279, 866
Ppu21I YACGTR 1 cut(s) 1183
PshBI ATTAAT 1 cut(s) 1352
PsiI TTATAA 1 cut(s) 1041
Psp6I CCWGG 5 cut(s) 201, 554, 570, 699, 955
PspEI GGTNACC 1 cut(s) 695
PspFI CCCAGC 1 cut(s) 291
PspGI CCWGG 5 cut(s) 201, 554, 570, 699, 955
PspLI CGTACG 1 cut(s) 1183
PspN4I GGNNCC 2 cut(s) 691, 828
PspPI GGNCC 2 cut(s) 481, 826
PsrI GAACNNNNNNTAC 2 cut(s) 839, 871
PstI CTGCAG 1 cut(s) 1330
PstNI CAGNNNCTG 1 cut(s) 61
PsyI GACNNNGTC 1 cut(s) 752
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 7 cut(s) 156, 297, 360, 597, 782, 983, 1352
SatI GCNGC 2 cut(s) 266, 333
Sau3AI GATC 6 cut(s) 277, 307, 664, 705, 1000, 1107
Sau96I GGNCC 2 cut(s) 481, 826
ScaI AGTACT 3 cut(s) 610, 848, 915
SchI GAGTC 2 cut(s) 279, 866
ScrFI CCNGG 6 cut(s) 203, 556, 572, 615, 701, 957
SduI GDGCHC 1 cut(s) 745
SexAI ACCWGGT 1 cut(s) 570
SfaNI GCATC 3 cut(s) 210, 454, 806
SfcI CTRYAG 1 cut(s) 1326
SinI GGWCC 2 cut(s) 481, 826
SmlI CTYRAG 1 cut(s) 982
SmoI CTYRAG 1 cut(s) 982
SnaBI TACGTA 1 cut(s) 1183
SpeI ACTAGT 1 cut(s) 605
Sse9I AATT 7 cut(s) 354, 476, 835, 1130, 1349, 1353, 1366
SsiI CCGC 4 cut(s) 723, 894, 1142, 1336
SspMI CTAG 3 cut(s) 548, 606, 684
StyD4I CCNGG 6 cut(s) 201, 554, 570, 613, 699, 955
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 4 cut(s) 421, 635, 778, 1361
TaiI ACGT 4 cut(s) 630, 1059, 1185, 1189
TaqI TCGA 6 cut(s) 28, 393, 708, 1322, 1370, 1406
TaqII GACCGA 1 cut(s) 419
TasI AATT 7 cut(s) 354, 476, 835, 1130, 1349, 1353, 1366
TatI WGTACW 7 cut(s) 314, 608, 846, 913, 1174, 1244, 1343
TfiI GAWTC 3 cut(s) 52, 640, 1400
Tru1I TTAA 7 cut(s) 156, 297, 360, 597, 782, 983, 1352
Tru9I TTAA 7 cut(s) 156, 297, 360, 597, 782, 983, 1352
TscAI CASTG 3 cut(s) 640, 946, 1196
TseFI GTSAC 3 cut(s) 400, 695, 1187
TseI GCWGC 2 cut(s) 265, 332
Tsp45I GTSAC 3 cut(s) 400, 695, 1187
TspDTI ATGAA 4 cut(s) 21, 314, 530, 1392
TspGWI ACGGA 2 cut(s) 473, 615
TspRI CASTG 3 cut(s) 640, 946, 1196
Tth111I GACNNNGTC 1 cut(s) 752
Vha464I CTTAAG 1 cut(s) 982
VpaK11BI GGWCC 2 cut(s) 481, 826
VspI ATTAAT 1 cut(s) 1352
XapI RAATTY 1 cut(s) 835
XceI RCATGY 1 cut(s) 1387
XcmI CCANNNNNNNNNTGG 2 cut(s) 244, 963
XspI CTAG 3 cut(s) 548, 606, 684
ZrmI AGTACT 3 cut(s) 610, 848, 915
Zsp2I ATGCAT 2 cut(s) 225, 1385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.