Rorug01G0415400

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003100
Physical Location & Seq
Forward (+)
51876851 .. 51879261
2411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0415400.1

Sequence Viewer

Length: 1371 bp
ATGGATCAAAGGCTGTTTGAGGCTTCTCAAAGTGGAAATATCCAACTTCTGCACCAGTTGCTTGCAGAGAATCCACTCCTACTCCATAGTCTTGCTTTAGATTCTGCAGAGAACCCCCTACATGTTGCTTCGCTTGCTGACCATGTTGGCTTTGTTAAGGAGATTCTAAGGTTGAAATCGACTTTGGCCAAAGAAATGAACCCAGATGGCTTTAGCCCTATGCATAATATAGCATCTGCCAACGGATATGTGGAGATTGTAGGGGAGCTTCTGAAAGTTGATCTAAGACTATGCCGGCTTAAGGGAAGAGATCAATGGACTCCTCTTCATCATGCTGCAAGGAGAGGAAGGGTAGATATTGTCAGAGAAATAGTCTTGGTTTGTCCAGAAAGTGTTGAAGATGTGACCATACAAGGGGAAACTGCTTTGCACCTTGCTGTTAAGAACAGTCAGTTTGAAGCTGTTGAAGTTTTGGTGAAATCGGTCACAGAAATGAGGAAGGTGAACATCCTGAATTTGAAGGATAAGCATGGCAATACAGTTCTTCACCTAGCAACATGGAGAAAACAACACCAGGTGGTGGAATGGTTAATTGGAATTGGAGCTAGTACCCCAGACGCAATGGAAGTGAACAACGTAAACCAGAGTGGTCTCACTGCTCTTGATCTTCTACTACTTTTCCCAAGTGAAGCTGGTGATCGGGAAATCTACGACACCCTTCGTGGTGCTGGAGCTTTGAGAGCACAAGACATGGTTCATTCTGCTATACCTTCATTTGACTCTGATAACCAAACCCTCTTGAACTGCCCCGTGGCATCAGGGACACATCAGTTGCAGCATCCAAATGATTTCATCGAATACTTCAAGTTCAAAAAGGGCAGAGATTCTCCTAGTGATGCGCGCACCGCGCTGTTAGTTGTAGCCGTATTGGTGACTACAGCGACATTTCAAGTGGGACTAAGCCCTCCAACCGGTGTTTGGCAGGACACCGAGCTGAAAAATAATGGGACTAGCAATGAACCAACACACAATGCAGGAACGTCTATAATGGGGTCTTATAACGCAGCTGCATTTGTGGTTTTCGTGGCTTTCAACTCCATTGGTTTTTCGGTTTCTCTTTACATGATCAACATCCTCACAAGCAACTTCCCTTTGCAGTTGGAGCTTCAGATCTGTATCATTGCCATGTATTGTACATACAACGCAGCGATGATTAACATTGCACCGAATAACACTGAAGTCTTCATTATTGTATTTACTTCGGTTTTGCCTGCTGCGGTATCACTTGCTGCCAAATCGGTTAGGCAACTTGTTATGAGGCTTGGAGAACCTCTGGTAAATTCAGCGCAGAGATTCATTCATTCGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

456

Amino Acids

50.04

Weight (kDa)

5.97

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 92 3.2e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 41 - 124 2e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 77 - 161 8.1e-14 Ankyrin repeats (3 copies)
Ank_2 PF12796 135 - 202 1.6e-10 Ankyrin repeats (3 copies)
PGG PF13962 299 - 401 2.3e-16 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1059
AccB7I CCANNNNNTGG 1 cut(s) 580
AccII CGCG 2 cut(s) 901, 908
AciI CCGC 2 cut(s) 906, 1277
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 2 cut(s) 514, 1339
AcuI CTGAAG 2 cut(s) 1151, 1257
AdeI CACNNNGTG 1 cut(s) 577
AfaI GTAC 2 cut(s) 610, 1195
AfiI CCNNNNNNNGG 5 cut(s) 301, 414, 580, 971, 978
AflII CTTAAG 1 cut(s) 299
AflIII ACRYGT 1 cut(s) 121
AgeI ACCGGT 1 cut(s) 971
AjnI CCWGG 1 cut(s) 573
AjuI GAANNNNNNNTTGG 4 cut(s) 167, 199, 576, 608
AluBI AGCT 8 cut(s) 268, 461, 605, 692, 734, 994, 1067, 1165
AluI AGCT 8 cut(s) 268, 461, 605, 692, 734, 994, 1067, 1165
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 1 cut(s) 656
AlwI GGATC 1 cut(s) 12
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 7 cut(s) 335, 835, 1064, 1067, 1205, 1274, 1289
ApoI RAATTY 2 cut(s) 514, 1339
AsiGI ACCGGT 1 cut(s) 971
AspLEI GCGC 4 cut(s) 901, 903, 910, 1348
AsuHPI GGTGA 5 cut(s) 487, 514, 539, 707, 943
AsuII TTCGAA 1 cut(s) 1364
BalI TGGCCA 1 cut(s) 188
BarI GAAGNNNNNNTAC 2 cut(s) 252, 284
BbsI GAAGAC 1 cut(s) 1234
Bbv12I GWGCWC 1 cut(s) 745
BbvI GCAGC 7 cut(s) 322, 847, 1054, 1076, 1217, 1261, 1276
BccI CCATC 1 cut(s) 200
BceAI ACGGC 1 cut(s) 908
BciT130I CCWGG 1 cut(s) 575
BclI TGATCA 1 cut(s) 1125
BcoDI GTCTC 1 cut(s) 656
BfaI CTAG 5 cut(s) 551, 606, 891, 1011, 1369
BfmI CTRYAG 2 cut(s) 105, 936
BfrI CTTAAG 1 cut(s) 299
BglII AGATCT 1 cut(s) 1170
BisI GCNGC 7 cut(s) 336, 836, 1065, 1068, 1206, 1275, 1290
BlsI GCNGC 7 cut(s) 337, 837, 1066, 1069, 1207, 1276, 1291
Bme1390I CCNGG 1 cut(s) 575
BmrFI CCNGG 1 cut(s) 575
BmsI GCATC 4 cut(s) 242, 824, 847, 886
BpiI GAAGAC 1 cut(s) 1234
BpmI CTGGAG 1 cut(s) 750
Bpu14I TTCGAA 1 cut(s) 1364
BsaBI GATNNNNATC 2 cut(s) 1130, 1175
BsaI GGTCTC 1 cut(s) 656
BsaJI CCNNGG 1 cut(s) 810
BsaWI WCCGGW 1 cut(s) 971
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 5 cut(s) 301, 414, 580, 971, 978
Bse118I RCCGGY 2 cut(s) 294, 971
Bse1I ACTGG 1 cut(s) 55
Bse3DI GCAATG 4 cut(s) 627, 1021, 1179, 1218
Bse8I GATNNNNATC 2 cut(s) 1130, 1175
BseBI CCWGG 1 cut(s) 575
BseDI CCNNGG 1 cut(s) 810
BseGI GGATG 3 cut(s) 507, 838, 1131
BseJI GATNNNNATC 2 cut(s) 1130, 1175
BseLI CCNNNNNNNGG 5 cut(s) 301, 414, 580, 971, 978
BseMI GCAATG 4 cut(s) 627, 1021, 1179, 1218
BseNI ACTGG 1 cut(s) 55
BsePI GCGCGC 1 cut(s) 899
BseRI GAGGAG 1 cut(s) 312
BseXI GCAGC 7 cut(s) 322, 847, 1054, 1076, 1217, 1261, 1276
BsgI GTGCAG 1 cut(s) 35
Bsh1236I CGCG 2 cut(s) 901, 908
BshFI GGCC 1 cut(s) 188
BshTI ACCGGT 1 cut(s) 971
BsiHKAI GWGCWC 1 cut(s) 745
BsiSI CCGG 2 cut(s) 295, 972
BslFI GGGAC 3 cut(s) 835, 969, 1021
BslI CCNNNNNNNGG 5 cut(s) 301, 414, 580, 971, 978
BsmAI GTCTC 1 cut(s) 656
BsmFI GGGAC 3 cut(s) 835, 969, 1021
BsnI GGCC 1 cut(s) 188
Bso31I GGTCTC 1 cut(s) 656
Bsp119I TTCGAA 1 cut(s) 1364
Bsp1286I GDGCHC 1 cut(s) 745
Bsp1407I TGTACA 1 cut(s) 1193
Bsp143I GATC 7 cut(s) 4, 280, 310, 664, 697, 1125, 1170
BspACI CCGC 2 cut(s) 906, 1277
BspANI GGCC 1 cut(s) 188
BspFNI CGCG 2 cut(s) 901, 908
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 1364
BspTI CTTAAG 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 656
BsrDI GCAATG 4 cut(s) 627, 1021, 1179, 1218
BsrFI RCCGGY 2 cut(s) 294, 971
BsrGI TGTACA 1 cut(s) 1193
BsrI ACTGG 1 cut(s) 55
BssAI RCCGGY 2 cut(s) 294, 971
BssECI CCNNGG 1 cut(s) 810
BssHII GCGCGC 1 cut(s) 899
BssMI GATC 7 cut(s) 4, 280, 310, 664, 697, 1125, 1170
Bst2UI CCWGG 1 cut(s) 575
Bst4CI ACNGT 2 cut(s) 449, 541
Bst6I CTCTTC 2 cut(s) 301, 330
BstAFI CTTAAG 1 cut(s) 299
BstAPI GCANNNNNTGC 1 cut(s) 58
BstAUI TGTACA 1 cut(s) 1193
BstBI TTCGAA 1 cut(s) 1364
BstC8I GCNNGC 5 cut(s) 63, 135, 296, 901, 1272
BstDEI CTNAG 3 cut(s) 167, 284, 959
BstDSI CCRYGG 1 cut(s) 810
BstF5I GGATG 3 cut(s) 507, 838, 1131
BstFNI CGCG 2 cut(s) 901, 908
BstHHI GCGC 4 cut(s) 901, 903, 910, 1348
BstKTI GATC 7 cut(s) 7, 283, 313, 667, 700, 1128, 1173
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 7 cut(s) 4, 280, 310, 664, 697, 1125, 1170
BstMWI GCNNNNNNNGC 6 cut(s) 58, 134, 740, 905, 907, 1162
BstNI CCWGG 1 cut(s) 575
BstNSI RCATGY 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 573
BstSFI CTRYAG 2 cut(s) 105, 936
BstUI CGCG 2 cut(s) 901, 908
BstV1I GCAGC 7 cut(s) 322, 847, 1054, 1076, 1217, 1261, 1276
BstV2I GAAGAC 1 cut(s) 1234
BstX2I RGATCY 1 cut(s) 1170
BstYI RGATCY 1 cut(s) 1170
BsuRI GGCC 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 810
BtgZI GCGATG 1 cut(s) 1223
BtsCI GGATG 3 cut(s) 507, 838, 1131
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 2 cut(s) 654, 1233
Cac8I GCNNGC 5 cut(s) 63, 135, 296, 901, 1272
CfoI GCGC 4 cut(s) 901, 903, 910, 1348
Cfr10I RCCGGY 2 cut(s) 294, 971
CseI GACGC 1 cut(s) 626
CsiI ACCWGGT 1 cut(s) 573
Csp6I GTAC 2 cut(s) 609, 1194
CspAI ACCGGT 1 cut(s) 971
CviAII CATG 8 cut(s) 122, 143, 332, 530, 558, 751, 1123, 1186
CviQI GTAC 2 cut(s) 609, 1194
DdeI CTNAG 3 cut(s) 167, 284, 959
DpnI GATC 7 cut(s) 6, 282, 312, 666, 699, 1127, 1172
DpnII GATC 7 cut(s) 4, 280, 310, 664, 697, 1125, 1170
DraIII CACNNNGTG 1 cut(s) 577
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 2 cut(s) 301, 330
EarI CTCTTC 2 cut(s) 301, 330
Eco31I GGTCTC 1 cut(s) 656
Eco57I CTGAAG 2 cut(s) 1151, 1257
EcoRII CCWGG 1 cut(s) 573
EcoT22I ATGCAT 1 cut(s) 225
FaeI CATG 8 cut(s) 125, 146, 335, 533, 561, 754, 1126, 1189
FaqI GGGAC 3 cut(s) 835, 969, 1021
FatI CATG 8 cut(s) 121, 142, 331, 529, 557, 750, 1122, 1185
FbaI TGATCA 1 cut(s) 1125
Fnu4HI GCNGC 7 cut(s) 336, 836, 1065, 1068, 1206, 1275, 1290
FokI GGATG 3 cut(s) 494, 825, 1118
Fsp4HI GCNGC 7 cut(s) 336, 836, 1065, 1068, 1206, 1275, 1290
FspBI CTAG 5 cut(s) 551, 606, 891, 1011, 1369
GlaI GCGC 4 cut(s) 900, 902, 909, 1347
GluI GCNGC 7 cut(s) 336, 836, 1065, 1068, 1206, 1275, 1290
GsuI CTGGAG 1 cut(s) 750
HaeIII GGCC 1 cut(s) 188
HapII CCGG 2 cut(s) 295, 972
HgaI GACGC 1 cut(s) 626
HhaI GCGC 4 cut(s) 901, 903, 910, 1348
Hin1II CATG 8 cut(s) 125, 146, 335, 533, 561, 754, 1126, 1189
Hin6I GCGC 4 cut(s) 899, 901, 908, 1346
HinP1I GCGC 4 cut(s) 899, 901, 908, 1346
HinfI GANTC 7 cut(s) 70, 101, 163, 319, 779, 884, 1353
HpaII CCGG 2 cut(s) 295, 972
HphI GGTGA 5 cut(s) 487, 514, 539, 707, 943
Hpy166II GTNNAC 3 cut(s) 505, 631, 640
Hpy188I TCNGA 4 cut(s) 273, 365, 784, 1170
Hpy188III TCNNGA 5 cut(s) 386, 511, 662, 701, 799
Hpy8I GTNNAC 3 cut(s) 505, 631, 640
HpyAV CCTTC 5 cut(s) 342, 493, 514, 728, 780
HpyCH4III ACNGT 2 cut(s) 449, 541
HpyCH4IV ACGT 2 cut(s) 636, 1040
HpyF10VI GCNNNNNNNGC 6 cut(s) 58, 134, 740, 905, 907, 1162
HpyF3I CTNAG 3 cut(s) 167, 284, 959
HpySE526I ACGT 2 cut(s) 636, 1040
Hsp92II CATG 8 cut(s) 125, 146, 335, 533, 561, 754, 1126, 1189
HspAI GCGC 4 cut(s) 899, 901, 908, 1346
KroI GCCGGC 1 cut(s) 294
KroNI GCCGGC 1 cut(s) 296
Ksp22I TGATCA 1 cut(s) 1125
Kzo9I GATC 7 cut(s) 4, 280, 310, 664, 697, 1125, 1170
LmnI GCTCC 4 cut(s) 265, 602, 731, 1162
Lsp1109I GCAGC 7 cut(s) 322, 847, 1054, 1076, 1217, 1261, 1276
LweI GCATC 4 cut(s) 242, 824, 847, 886
MabI ACCWGGT 1 cut(s) 573
MaeI CTAG 5 cut(s) 551, 606, 891, 1011, 1369
MaeII ACGT 2 cut(s) 636, 1040
MaeIII GTNAC 3 cut(s) 403, 484, 931
MalI GATC 7 cut(s) 6, 282, 312, 666, 699, 1127, 1172
MboI GATC 7 cut(s) 4, 280, 310, 664, 697, 1125, 1170
MboII GAAGA 6 cut(s) 317, 318, 410, 536, 659, 1234
MflI RGATCY 1 cut(s) 1170
MhlI GDGCHC 1 cut(s) 745
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 4 cut(s) 514, 591, 597, 1339
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 2 cut(s) 313, 773
MmeI TCCRAC 3 cut(s) 67, 992, 1140
MnlI CCTC 9 cut(s) 13, 333, 338, 489, 806, 975, 1145, 1311, 1341
Mox20I TGGCCA 1 cut(s) 188
Mph1103I ATGCAT 1 cut(s) 225
MroNI GCCGGC 1 cut(s) 294
MscI TGGCCA 1 cut(s) 188
MseI TTAA 5 cut(s) 156, 300, 441, 590, 1215
MslI CAYNNNNRTG 3 cut(s) 491, 843, 1184
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 1067
MspCI CTTAAG 1 cut(s) 299
MspI CCGG 2 cut(s) 295, 972
MspR9I CCNGG 1 cut(s) 575
MvaI CCWGG 1 cut(s) 575
MvnI CGCG 2 cut(s) 901, 908
MwoI GCNNNNNNNGC 6 cut(s) 58, 134, 740, 905, 907, 1162
NaeI GCCGGC 1 cut(s) 296
NdeII GATC 7 cut(s) 4, 280, 310, 664, 697, 1125, 1170
NgoMIV GCCGGC 1 cut(s) 294
NlaIII CATG 8 cut(s) 125, 146, 335, 533, 561, 754, 1126, 1189
NmuCI GTSAC 3 cut(s) 403, 484, 931
NsiI ATGCAT 1 cut(s) 225
NspI RCATGY 1 cut(s) 125
NspV TTCGAA 1 cut(s) 1364
PauI GCGCGC 1 cut(s) 899
PciI ACATGT 1 cut(s) 121
PdiI GCCGGC 1 cut(s) 296
PfeI GAWTC 5 cut(s) 70, 101, 163, 884, 1353
PflMI CCANNNNNTGG 1 cut(s) 580
PinAI ACCGGT 1 cut(s) 971
PkrI GCNGC 7 cut(s) 337, 837, 1066, 1069, 1207, 1276, 1291
PleI GAGTC 2 cut(s) 313, 773
PpsI GAGTC 2 cut(s) 313, 773
PscI ACATGT 1 cut(s) 121
PsiI TTATAA 1 cut(s) 1059
Psp6I CCWGG 1 cut(s) 573
PspGI CCWGG 1 cut(s) 573
PstI CTGCAG 1 cut(s) 109
PsuI RGATCY 1 cut(s) 1170
PteI GCGCGC 1 cut(s) 899
PvuII CAGCTG 1 cut(s) 1067
RsaI GTAC 2 cut(s) 610, 1195
RsaNI GTAC 2 cut(s) 609, 1194
RseI CAYNNNNRTG 3 cut(s) 491, 843, 1184
SaqAI TTAA 5 cut(s) 156, 300, 441, 590, 1215
SatI GCNGC 7 cut(s) 336, 836, 1065, 1068, 1206, 1275, 1290
Sau3AI GATC 7 cut(s) 4, 280, 310, 664, 697, 1125, 1170
SchI GAGTC 2 cut(s) 313, 773
ScrFI CCNGG 1 cut(s) 575
SduI GDGCHC 1 cut(s) 745
SexAI ACCWGGT 1 cut(s) 573
SfaNI GCATC 4 cut(s) 242, 824, 847, 886
SfcI CTRYAG 2 cut(s) 105, 936
SfuI TTCGAA 1 cut(s) 1364
SmiMI CAYNNNNRTG 3 cut(s) 491, 843, 1184
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 4 cut(s) 514, 591, 597, 1339
SsiI CCGC 2 cut(s) 906, 1277
SspMI CTAG 5 cut(s) 551, 606, 891, 1011, 1369
StyD4I CCNGG 1 cut(s) 573
TaaI ACNGT 2 cut(s) 449, 541
TaiI ACGT 2 cut(s) 639, 1043
TaqI TCGA 3 cut(s) 179, 855, 1364
TaqII GACCGA 1 cut(s) 472
TasI AATT 4 cut(s) 514, 591, 597, 1339
TatI WGTACW 1 cut(s) 1193
TfiI GAWTC 5 cut(s) 70, 101, 163, 884, 1353
Tru1I TTAA 5 cut(s) 156, 300, 441, 590, 1215
Tru9I TTAA 5 cut(s) 156, 300, 441, 590, 1215
TscAI CASTG 2 cut(s) 661, 1240
TseFI GTSAC 3 cut(s) 403, 484, 931
TseI GCWGC 7 cut(s) 335, 835, 1064, 1067, 1205, 1274, 1289
Tsp45I GTSAC 3 cut(s) 403, 484, 931
TspDTI ATGAA 9 cut(s) 212, 317, 746, 762, 841, 1032, 1234, 1345, 1349
TspGWI ACGGA 1 cut(s) 258
TspRI CASTG 2 cut(s) 661, 1240
Van91I CCANNNNNTGG 1 cut(s) 580
Vha464I CTTAAG 1 cut(s) 299
XapI RAATTY 2 cut(s) 514, 1339
XceI RCATGY 1 cut(s) 125
XcmI CCANNNNNNNNNTGG 2 cut(s) 247, 975
XspI CTAG 5 cut(s) 551, 606, 891, 1011, 1369
Zsp2I ATGCAT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.