MD11G1052200.v1.1

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003100
Physical Location & Seq
Forward (+)
4457598 .. 4467093
9496 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1052200.v1.1.491

Sequence Viewer

Length: 855 bp
ATGTCAGTAGTTCCTCTCTCCAATCGCCGCCCAAACATTATGGCGGACGACAATCAGAATGAGGTGAAGGACGAGGTGGTAGCAGATATTGCTCCCTTTGATCCTACTAAAAAGAAGAAAAAGAAGAAGGTTGTTATTCAGGATACTACTGATGATTCTGTGGACAAGTTGGCCGAGAAAACGGAAACCTTGACAGTTTCTGAGGGGCAGGAGAGTACGTTTACTGGTTTGAAAAAGAAGAAGAAGAAGCCAGTTGAGACCAGTATCTTGAATGAAGAGAGTGGTGACCCAGTGGAAGATGTGAATGAGCACACTGGTGAGGATGAGGAAGGAGAAGGGATTGTTCTAGAGACTCCATCATATCCTTGGGAAGGGAGTGATCGTGATTACACTTATGAAGAACTTCTTGATAGGGTGTTTAACATTCTTCGCGAAAATAACCCGGATCTGGCTGGAGACAGGCGTAGGACAGTGATGAGGCCTCCACAAGTTCTTCGTGAGGGCACAAAGAAAACCGTTTTTGTGAATTTCATGGATCTATGCAAGACGATGCATAGGCAACCAGATCATGTCATGGCTTTCTTGCTTGCCGAACTGGGAACTAGTGGATCACTTGATGGACAGCAGAGGTTGGTTGTTAAGGGAAGATTTGCCCCAAAAAATTTCGAAGGAATACTGCGGCGATATGTCAATGAATATGTCATTTGCCTCGGGTGCAAAAGTCCAGACACTATACTTTCAAAGGAGAATCGTCTCTTCTTTCTCAGATGCGAGAAGTGTGGTTCTGGACGATCAGTTGCTCCAATTAAAGCTGGTTTTATGGCTCGTGTTGGGCGAAGGAACGCCGGGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

285

Amino Acids

32.01

Weight (kDa)

6.4

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-5_eIF-2B PF01873 153 - 262 1.2e-39 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 432
AciI CCGC 3 cut(s) 28, 44, 679
AclWI GGATC 4 cut(s) 95, 453, 543, 616
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 2 cut(s) 526, 661
AfaI GTAC 1 cut(s) 217
AfiI CCNNNNNNNGG 2 cut(s) 371, 448
AgsI TTSAA 3 cut(s) 232, 271, 741
AhlI ACTAGT 1 cut(s) 602
AleI CACNNNNGTG 1 cut(s) 315
AluBI AGCT 1 cut(s) 812
AluI AGCT 1 cut(s) 812
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 4 cut(s) 251, 344, 450, 758
AlwI GGATC 4 cut(s) 95, 453, 543, 616
AlwNI CAGNNNCTG 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 710
AoxI GGCC 2 cut(s) 171, 479
ApoI RAATTY 2 cut(s) 526, 661
Asp700I GAANNNNTTC 1 cut(s) 402
AsuC2I CCSGG 2 cut(s) 443, 847
AsuHPI GGTGA 3 cut(s) 76, 296, 329
AsuII TTCGAA 1 cut(s) 666
AvaI CYCGRG 1 cut(s) 710
BaeGI GKGCMC 1 cut(s) 506
BauI CACGAG 1 cut(s) 825
Bbv12I GWGCWC 1 cut(s) 312
BccI CCATC 2 cut(s) 364, 611
BciVI GTATCC 1 cut(s) 136
BcnI CCSGG 2 cut(s) 443, 847
BcoDI GTCTC 4 cut(s) 251, 344, 450, 758
BcuI ACTAGT 1 cut(s) 602
BfaI CTAG 2 cut(s) 347, 603
BfuI GTATCC 1 cut(s) 136
BisI GCNGC 2 cut(s) 28, 680
BlsI GCNGC 2 cut(s) 29, 681
Bme1390I CCNGG 2 cut(s) 443, 847
BmeT110I CYCGRG 1 cut(s) 710
BmrFI CCNGG 2 cut(s) 443, 847
BmrI ACTGGG 2 cut(s) 284, 605
BmsI GCATC 2 cut(s) 540, 758
BmuI ACTGGG 2 cut(s) 284, 605
BpmI CTGGAG 1 cut(s) 474
Bpu14I TTCGAA 1 cut(s) 666
BpuMI CCSGG 2 cut(s) 443, 847
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 2 cut(s) 365, 709
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 367, 397
Bsc4I CCNNNNNNNGG 2 cut(s) 371, 448
Bse1I ACTGG 6 cut(s) 229, 251, 261, 290, 319, 600
BseDI CCNNGG 2 cut(s) 365, 709
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 2 cut(s) 371, 448
BseMII CTCAG 2 cut(s) 192, 778
BseNI ACTGG 6 cut(s) 229, 251, 261, 290, 319, 600
BseSI GKGCMC 1 cut(s) 506
Bsh1236I CGCG 1 cut(s) 432
BshFI GGCC 2 cut(s) 173, 481
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 710
BsiSI CCGG 2 cut(s) 443, 846
BslI CCNNNNNNNGG 2 cut(s) 371, 448
BsmAI GTCTC 4 cut(s) 251, 344, 450, 758
BsmBI CGTCTC 1 cut(s) 758
BsnI GGCC 2 cut(s) 173, 481
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 710
Bsp119I TTCGAA 1 cut(s) 666
Bsp1286I GDGCHC 2 cut(s) 312, 506
Bsp143I GATC 7 cut(s) 100, 379, 445, 535, 565, 608, 791
Bsp68I TCGCGA 1 cut(s) 432
BspACI CCGC 3 cut(s) 28, 44, 679
BspANI GGCC 2 cut(s) 173, 481
BspCNI CTCAG 2 cut(s) 193, 777
BspFNI CGCG 1 cut(s) 432
BspPI GGATC 4 cut(s) 95, 453, 543, 616
BspT104I TTCGAA 1 cut(s) 666
BspTNI GGTCTC 1 cut(s) 251
BsrI ACTGG 6 cut(s) 229, 251, 261, 290, 319, 600
BssECI CCNNGG 2 cut(s) 365, 709
BssMI GATC 7 cut(s) 100, 379, 445, 535, 565, 608, 791
BssSI CACGAG 1 cut(s) 825
BssT1I CCWWGG 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 825
Bst4CI ACNGT 3 cut(s) 196, 472, 517
Bst6I CTCTTC 2 cut(s) 270, 761
BstAPI GCANNNNNTGC 1 cut(s) 89
BstBI TTCGAA 1 cut(s) 666
BstC8I GCNNGC 1 cut(s) 588
BstDEI CTNAG 2 cut(s) 201, 764
BstEII GGTNACC 1 cut(s) 284
BstENI CCTNNNNNAGG 1 cut(s) 369
BstF5I GGATG 1 cut(s) 328
BstFNI CGCG 1 cut(s) 432
BstKTI GATC 7 cut(s) 103, 382, 448, 538, 568, 611, 794
BstMAI GTCTC 4 cut(s) 251, 344, 450, 758
BstMBI GATC 7 cut(s) 100, 379, 445, 535, 565, 608, 791
BstMWI GCNNNNNNNGC 2 cut(s) 89, 714
BstPI GGTNACC 1 cut(s) 284
BstSCI CCNGG 2 cut(s) 441, 845
BstSLI GKGCMC 1 cut(s) 506
BstUI CGCG 1 cut(s) 432
BstX2I RGATCY 2 cut(s) 445, 535
BstYI RGATCY 2 cut(s) 445, 535
BsuI GTATCC 1 cut(s) 136
BsuRI GGCC 2 cut(s) 173, 481
BtsCI GGATG 1 cut(s) 328
BtsIMutI CAGTG 3 cut(s) 297, 312, 477
BtuMI TCGCGA 1 cut(s) 432
Cac8I GCNNGC 1 cut(s) 588
CaiI CAGNNNCTG 1 cut(s) 200
Csp6I GTAC 1 cut(s) 216
CviAII CATG 3 cut(s) 532, 569, 574
CviJI RGCY 7 cut(s) 173, 250, 452, 481, 578, 812, 824
CviKI_1 RGCY 7 cut(s) 173, 250, 452, 481, 578, 812, 824
CviQI GTAC 1 cut(s) 216
DdeI CTNAG 2 cut(s) 201, 764
DpnI GATC 7 cut(s) 102, 381, 447, 537, 567, 610, 793
DpnII GATC 7 cut(s) 100, 379, 445, 535, 565, 608, 791
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 2 cut(s) 270, 761
EarI CTCTTC 2 cut(s) 270, 761
EciI GGCGGA 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 365
Eco147I AGGCCT 1 cut(s) 481
Eco31I GGTCTC 1 cut(s) 251
Eco88I CYCGRG 1 cut(s) 710
Eco91I GGTNACC 1 cut(s) 284
EcoNI CCTNNNNNAGG 1 cut(s) 369
EcoO65I GGTNACC 1 cut(s) 284
EcoT14I CCWWGG 1 cut(s) 365
EcoT22I ATGCAT 1 cut(s) 555
ErhI CCWWGG 1 cut(s) 365
Esp3I CGTCTC 1 cut(s) 758
FaeI CATG 3 cut(s) 535, 572, 577
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FatI CATG 3 cut(s) 531, 568, 573
Fnu4HI GCNGC 2 cut(s) 28, 680
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 2 cut(s) 28, 680
FspBI CTAG 2 cut(s) 347, 603
GluI GCNGC 2 cut(s) 28, 680
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 2 cut(s) 173, 481
HapII CCGG 2 cut(s) 443, 846
Hin1II CATG 3 cut(s) 535, 572, 577
HinfI GANTC 3 cut(s) 155, 352, 748
HpaII CCGG 2 cut(s) 443, 846
HphI GGTGA 3 cut(s) 76, 296, 329
Hpy166II GTNNAC 2 cut(s) 163, 222
Hpy188I TCNGA 3 cut(s) 57, 202, 767
Hpy188III TCNNGA 9 cut(s) 140, 268, 347, 383, 407, 431, 497, 725, 786
Hpy8I GTNNAC 2 cut(s) 163, 222
HpyAV CCTTC 7 cut(s) 61, 121, 323, 329, 365, 662, 831
HpyCH4III ACNGT 3 cut(s) 196, 472, 517
HpyCH4IV ACGT 1 cut(s) 218
HpyCH4V TGCA 3 cut(s) 543, 553, 717
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 714
HpyF3I CTNAG 2 cut(s) 201, 764
HpySE526I ACGT 1 cut(s) 218
Hsp92II CATG 3 cut(s) 535, 572, 577
Kzo9I GATC 7 cut(s) 100, 379, 445, 535, 565, 608, 791
LmnI GCTCC 2 cut(s) 97, 805
LweI GCATC 2 cut(s) 540, 758
MaeI CTAG 2 cut(s) 347, 603
MaeII ACGT 1 cut(s) 218
MaeIII GTNAC 1 cut(s) 284
MalI GATC 7 cut(s) 102, 381, 447, 537, 567, 610, 793
MboI GATC 7 cut(s) 100, 379, 445, 535, 565, 608, 791
MflI RGATCY 2 cut(s) 445, 535
MhlI GDGCHC 2 cut(s) 312, 506
MluCI AATT 3 cut(s) 526, 661, 804
MlyI GAGTC 1 cut(s) 346
Mph1103I ATGCAT 1 cut(s) 555
MroXI GAANNNNTTC 1 cut(s) 402
MseI TTAA 3 cut(s) 420, 639, 807
MslI CAYNNNNRTG 1 cut(s) 315
MspI CCGG 2 cut(s) 443, 846
MspR9I CCNGG 2 cut(s) 443, 847
MvnI CGCG 1 cut(s) 432
MwoI GCNNNNNNNGC 2 cut(s) 89, 714
NciI CCSGG 2 cut(s) 443, 847
NdeII GATC 7 cut(s) 100, 379, 445, 535, 565, 608, 791
NlaIII CATG 3 cut(s) 535, 572, 577
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 284
NruI TCGCGA 1 cut(s) 432
NsiI ATGCAT 1 cut(s) 555
NspV TTCGAA 1 cut(s) 666
OliI CACNNNNGTG 1 cut(s) 315
PceI AGGCCT 1 cut(s) 481
PcsI WCGNNNNNNNCGW 1 cut(s) 832
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 155, 748
PkrI GCNGC 2 cut(s) 29, 681
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PspEI GGTNACC 1 cut(s) 284
PstNI CAGNNNCTG 1 cut(s) 200
PsuI RGATCY 2 cut(s) 445, 535
RruI TCGCGA 1 cut(s) 432
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
RseI CAYNNNNRTG 1 cut(s) 315
SaqAI TTAA 3 cut(s) 420, 639, 807
SatI GCNGC 2 cut(s) 28, 680
Sau3AI GATC 7 cut(s) 100, 379, 445, 535, 565, 608, 791
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 2 cut(s) 443, 847
SduI GDGCHC 2 cut(s) 312, 506
SetI ASST 7 cut(s) 66, 78, 132, 191, 221, 632, 814
SfaNI GCATC 2 cut(s) 540, 758
SfuI TTCGAA 1 cut(s) 666
SmiMI CAYNNNNRTG 1 cut(s) 315
SpeI ACTAGT 1 cut(s) 602
Sse9I AATT 3 cut(s) 526, 661, 804
SseBI AGGCCT 1 cut(s) 481
SsiI CCGC 3 cut(s) 28, 44, 679
SspMI CTAG 2 cut(s) 347, 603
StuI AGGCCT 1 cut(s) 481
StyD4I CCNGG 2 cut(s) 441, 845
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 3 cut(s) 196, 472, 517
TaiI ACGT 1 cut(s) 221
TaqI TCGA 1 cut(s) 666
TasI AATT 3 cut(s) 526, 661, 804
TauI GCSGC 2 cut(s) 30, 682
TfiI GAWTC 2 cut(s) 155, 748
Tru1I TTAA 3 cut(s) 420, 639, 807
Tru9I TTAA 3 cut(s) 420, 639, 807
TscAI CASTG 3 cut(s) 297, 319, 477
TseFI GTSAC 1 cut(s) 284
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 4 cut(s) 288, 411, 520, 708
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 3 cut(s) 297, 319, 477
XagI CCTNNNNNAGG 1 cut(s) 369
XapI RAATTY 2 cut(s) 526, 661
XbaI TCTAGA 1 cut(s) 346
XcmI CCANNNNNNNNNTGG 1 cut(s) 363
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 2 cut(s) 347, 603
Zsp2I ATGCAT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.