Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0003100

Physical Location & Seq

Genomic Coordinates Forward (+)

2523763 .. 2533152

Gene Structure 9390 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00279870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1530 bp

ATGGCCTTTTCTCGCTTACAAGATGGCCTTAAAGAGCAGGAGCTCGTGTTTTCTTTCGAAACAATCACTTCGGTACCATGTTTAAGATCAATGGAGAACTTTATCTTTTGGCAATCGATCGTGGTTACGAATCGGCATGATTTGGTGTGGGAAAAGCTTAATAAGGTGAATGGAGATACATTGTTCATGACCGGCAATTTCAAGGAATTTAAAGTTGAAAACCAATCAAATGAAAATTGGACCACCGCCGACTACCTTGCCGCATTGATATACTGTAGGGCTATGGTTAGGGAGCCGGATCATGTAATGAGTTTCATGCTTGCGGAATTGGACACGAGTGGATCGCTTGATGAACAGCTGAGGTTAGTTGTGAAGGGAAGATTTTTACCTAAGAAGTTCGGAGCAATCATGCGGAAATATGTCAATCAATATGTGATTTGCTTCGCATGCAAACGTACAGACACTCGACTTTCGAAAGAAAATCGGCCAAAGTACCATCTTATATGCGAGTGGTGTTGTGCTTCACGATCAGTTGCTCCTATTAAAGCTGGTTTTGTTGCCAAAGTTGGAAAAAGGAACAATGGGCTTGCCACTCTTCAAGTCCATGCCTTTGATAATGAAGAAAATGTGTTTTCGTCACTGGTGGGATTGCAATTCATGTGGCAGCTAGAAACCAATGAACCACTGCATCACCTTGTTCATGTTCCTCTTAAGGACTCTCCCCTGAGTGACTCTGGTGGATTGTGTGGAGACCTAGATGTCCAAATAAAACTTGAAGATAGTGGTGTATTCTCTGACATGTATGTTGTAAAAGGAATCGAAATTGGGCACGAGACTGTTTCTGTGCATTTGCTTGAGCCACAATACAAGCACATGGCTGATAAGATTGTCCTGACTGTAGCAGAAGCCATGTCACTTGATCCTCCCTCACCTGTACTTGTCCTCGTTGGCGCTGCTGTTCGTTATTATCTGAAAGTTATTCGTGTGAACAAGGCTCAAGTGCTAACTTGGCCGTCTCCACATCATCAATGGTCTTCATCAAACTCTTCAGTTGCTCATGTTGACTCTATGAAGGGTTTGACAAACGCATTGAGCCTAGGGGTAACAAATGCCATAGTTGAAGATACAAGGGTCAATGACGCTGCTGTAACAATGAAAGCATCAAATGGTGCCTACCTTTATAGATGTGATGCATTCAGTTCCTTGATAAAGTGGAAAGTAGAGAGTGGGCCTGTCATCATTGTCAAAGGGGAGGCATCTAATTTACACATGTTAGGGAATGCTGAGTTCCATACATCAAGTTATGGTTCTCCATGTTCATGGGCAGATTTATATGCTTCCGCTTCTGGTGGAGCTACTTTTCATGCTACATTGCCTAATGAATATCACAACTTTGAAAGCTCTTTTCATGGACCTATTGTCTTAAAAGCATCTTCACTTATTGCGGCATATCCACCACTTAATGGTCGTCAGTCTGGTGATGGAAATCATTATGGTGGTTACTTTTTTTATTTGACTCTTAACAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005783 GO:0012505 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Big_2 RRM_1

Protein Analysis

509

Amino Acids

56.66

Weight (kDa)

6.44

Isoelectric Point (pI)

31.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-5_eIF-2B	PF01873	91 - 174	6.8e-22	Domain found in IF2B/IF5
Ig_NUP210_2nd	PF22969	197 - 293	6.3e-13	NUP210 Ig-like domain 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003100)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07920
malus_domestica	MD03G1050000.v1.1 MD11G1052200.v1.1
prunus_persica	Prupe.6G039800_v2.0.a1
pyrus_communis	pycom03g03960 pycom11g04260
rosa_chinensis	RchiOBHm_Chr1g0379851 RchiOBHm_Chr1g0379891
rosa_laevigata	RLG00000026352 RLG00000026354
rosa_multiflora	Rmu_co8429969.1_g000001 Rmu_sc0004184.1_g000024 Rmu_sc0004184.1_g000025 Rmu_sc0004184.1_g000026 Rmu_sc0014259.1_g000013
rosa_roxburghii	Rroxscaffold_4G00279870 Rroxscaffold_4G00279880
rosa_rugosa	Rorug01G0415400 Rorug01G0415500 Rorug01G0415600
rosa_samantha	Rh1AG439500 Rh1AG439600 Rh1AG439700 Rh1BG395500 Rh1BG395600 Rh1BG395700 Rh1BG395800 Rh1CG408700 Rh1CG408800 Rh1CG408900 Rh1CG409400 Rh1DG426400 Rh1DG426500 Rh1DG426600 Rh1DG426700
rosa_wichuraiana	Rw1G038260 Rw1G038270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	758
Acc65I	GGTACC	1 cut(s)	73
AccB1I	GGYRCC	2 cut(s)	73, 1169
AccB7I	CCANNNNNTGG	1 cut(s)	1463
AciI	CCGC	6 cut(s)	246, 261, 323, 412, 1341, 1445
AclWI	GGATC	3 cut(s)	306, 349, 914
AcoI	YGGCCR	2 cut(s)	485, 1010
AcsI	RAATTY	1 cut(s)	206
AcuI	CTGAAG	1 cut(s)	1032
AfaI	GTAC	4 cut(s)	75, 457, 494, 936
AfiI	CCNNNNNNNGG	1 cut(s)	1463
AflII	CTTAAG	1 cut(s)	710
AflIII	ACRYGT	2 cut(s)	798, 1269
AgsI	TTSAA	6 cut(s)	202, 218, 599, 776, 1121, 1397
AhdI	GACNNNNNGTC	1 cut(s)	1418
AluBI	AGCT	8 cut(s)	43, 157, 358, 548, 667, 1355, 1401, 1527
AluI	AGCT	8 cut(s)	43, 157, 358, 548, 667, 1355, 1401, 1527
Alw21I	GWGCWC	1 cut(s)	45
Alw26I	GTCTC	3 cut(s)	744, 827, 1020
AlwI	GGATC	3 cut(s)	306, 349, 914
AoxI	GGCC	5 cut(s)	3, 25, 485, 1010, 1229
ApeKI	GCWGC	3 cut(s)	664, 953, 1142
ApoI	RAATTY	1 cut(s)	206
Asp718I	GGTACC	1 cut(s)	73
AspA2I	CCTAGG	1 cut(s)	1096
AspLEI	GCGC	1 cut(s)	953
AspS9I	GGNCC	3 cut(s)	240, 1229, 1412
AsuHPI	GGTGA	4 cut(s)	178, 683, 921, 1490
AsuII	TTCGAA	2 cut(s)	57, 473
AvaII	GGWCC	2 cut(s)	240, 1412
AvrII	CCTAGG	1 cut(s)	1096
BaeGI	GKGCMC	1 cut(s)	831
BaeI	ACNNNNGTAYC	4 cut(s)	57, 90, 1116, 1149
BanI	GGYRCC	2 cut(s)	73, 1169
BanII	GRGCYC	1 cut(s)	45
BauI	CACGAG	3 cut(s)	44, 334, 830
BbsI	GAAGAC	1 cut(s)	1026
Bbv12I	GWGCWC	1 cut(s)	45
BbvCI	CCTCAGC	1 cut(s)	359
BbvI	GCAGC	3 cut(s)	676, 940, 1129
BccI	CCATC	3 cut(s)	17, 504, 1475
BceAI	ACGGC	1 cut(s)	997
BcgI	CGANNNNNNTGC	2 cut(s)	239, 273
BcoDI	GTCTC	3 cut(s)	744, 827, 1020
BfaI	CTAG	4 cut(s)	668, 755, 1097, 1528
BfmI	CTRYAG	2 cut(s)	274, 897
BfoI	RGCGCY	1 cut(s)	954
BfrI	CTTAAG	1 cut(s)	710
BisI	GCNGC	5 cut(s)	261, 665, 954, 1143, 1446
BlnI	CCTAGG	1 cut(s)	1096
BlsI	GCNGC	5 cut(s)	262, 666, 955, 1144, 1447
Bme18I	GGWCC	2 cut(s)	240, 1412
BmeRI	GACNNNNNGTC	1 cut(s)	1418
BmgT120I	GGNCC	3 cut(s)	240, 1229, 1412
BmiI	GGNNCC	3 cut(s)	75, 294, 1171
BmsI	GCATC	5 cut(s)	697, 1169, 1180, 1265, 1439
BpiI	GAAGAC	1 cut(s)	1026
Bpu10I	CCTNAGC	1 cut(s)	359
Bpu14I	TTCGAA	2 cut(s)	57, 473
BpuEI	CTTGAG	2 cut(s)	875, 981
Bsa29I	ATCGAT	1 cut(s)	116
BsaBI	GATNNNNATC	1 cut(s)	1485
BsaI	GGTCTC	1 cut(s)	744
BsaJI	CCNNGG	1 cut(s)	1096
BsaXI	ACNNNNNCTCC	2 cut(s)	32, 62
Bsc4I	CCNNNNNNNGG	1 cut(s)	1463
Bse118I	RCCGGY	1 cut(s)	191
Bse1I	ACTGG	1 cut(s)	645
Bse3DI	GCAATG	1 cut(s)	1370
Bse8I	GATNNNNATC	1 cut(s)	1485
BseCI	ATCGAT	1 cut(s)	116
BseDI	CCNNGG	1 cut(s)	1096
BseJI	GATNNNNATC	1 cut(s)	1485
BseLI	CCNNNNNNNGG	1 cut(s)	1463
BseMI	GCAATG	1 cut(s)	1370
BseMII	CTCAG	3 cut(s)	350, 716, 1275
BseNI	ACTGG	1 cut(s)	645
BseSI	GKGCMC	1 cut(s)	831
BseXI	GCAGC	3 cut(s)	676, 940, 1129
Bsh1285I	CGRYCG	1 cut(s)	120
BshFI	GGCC	5 cut(s)	5, 27, 487, 1012, 1231
BshNI	GGYRCC	2 cut(s)	73, 1169
BshVI	ATCGAT	1 cut(s)	116
BsiEI	CGRYCG	1 cut(s)	120
BsiHKAI	GWGCWC	1 cut(s)	45
BsiSI	CCGG	2 cut(s)	192, 296
BslI	CCNNNNNNNGG	1 cut(s)	1463
BsmAI	GTCTC	3 cut(s)	744, 827, 1020
BsmBI	CGTCTC	1 cut(s)	1020
BsmI	GAATGC	2 cut(s)	1193, 1285
BsnI	GGCC	5 cut(s)	5, 27, 487, 1012, 1231
Bso31I	GGTCTC	1 cut(s)	744
Bsp119I	TTCGAA	2 cut(s)	57, 473
Bsp1286I	GDGCHC	2 cut(s)	45, 831
Bsp143I	GATC	6 cut(s)	86, 117, 298, 341, 527, 919
BspACI	CCGC	6 cut(s)	246, 261, 323, 412, 1341, 1445
BspANI	GGCC	5 cut(s)	5, 27, 487, 1012, 1231
BspCNI	CTCAG	3 cut(s)	351, 717, 1276
BspDI	ATCGAT	1 cut(s)	116
BspHI	TCATGA	1 cut(s)	186
BspLI	GGNNCC	3 cut(s)	75, 294, 1171
BspPI	GGATC	3 cut(s)	306, 349, 914
BspT104I	TTCGAA	2 cut(s)	57, 473
BspT107I	GGYRCC	2 cut(s)	73, 1169
BspTI	CTTAAG	1 cut(s)	710
BspTNI	GGTCTC	1 cut(s)	744
BsrDI	GCAATG	1 cut(s)	1370
BsrFI	RCCGGY	1 cut(s)	191
BsrI	ACTGG	1 cut(s)	645
BssAI	RCCGGY	1 cut(s)	191
BssECI	CCNNGG	1 cut(s)	1096
BssMI	GATC	6 cut(s)	86, 117, 298, 341, 527, 919
BssSI	CACGAG	3 cut(s)	44, 334, 830
BssT1I	CCWWGG	1 cut(s)	1096
Bst2BI	CACGAG	3 cut(s)	44, 334, 830
Bst4CI	ACNGT	3 cut(s)	275, 838, 898
Bst6I	CTCTTC	2 cut(s)	600, 1051
BstAFI	CTTAAG	1 cut(s)	710
BstBI	TTCGAA	2 cut(s)	57, 473
BstC8I	GCNNGC	3 cut(s)	321, 448, 588
BstDEI	CTNAG	4 cut(s)	359, 390, 725, 1284
BstH2I	RGCGCY	1 cut(s)	954
BstHHI	GCGC	1 cut(s)	953
BstKTI	GATC	6 cut(s)	89, 120, 301, 344, 530, 922
BstMAI	GTCTC	3 cut(s)	744, 827, 1020
BstMBI	GATC	6 cut(s)	86, 117, 298, 341, 527, 919
BstMCI	CGRYCG	1 cut(s)	120
BstMWI	GCNNNNNNNGC	2 cut(s)	447, 1009
BstNSI	RCATGY	3 cut(s)	450, 802, 1273
BstSFI	CTRYAG	2 cut(s)	274, 897
BstSLI	GKGCMC	1 cut(s)	831
BstV1I	GCAGC	3 cut(s)	676, 940, 1129
BstV2I	GAAGAC	1 cut(s)	1026
BstXI	CCANNNNNNTGG	1 cut(s)	1320
Bsu15I	ATCGAT	1 cut(s)	116
BsuRI	GGCC	5 cut(s)	5, 27, 487, 1012, 1231
BsuTUI	ATCGAT	1 cut(s)	116
BtsI	GCAGTG	1 cut(s)	683
BtsIMutI	CAGTG	2 cut(s)	638, 683
Cac8I	GCNNGC	3 cut(s)	321, 448, 588
CciI	TCATGA	1 cut(s)	186
CfoI	GCGC	1 cut(s)	953
Cfr10I	RCCGGY	1 cut(s)	191
Cfr13I	GGNCC	3 cut(s)	240, 1229, 1412
ClaI	ATCGAT	1 cut(s)	116
CseI	GACGC	1 cut(s)	1148
Csp6I	GTAC	4 cut(s)	74, 456, 493, 935
CspCI	CAANNNNNGTGG	2 cut(s)	641, 676
CviQI	GTAC	4 cut(s)	74, 456, 493, 935
DdeI	CTNAG	4 cut(s)	359, 390, 725, 1284
DpnI	GATC	6 cut(s)	88, 119, 300, 343, 529, 921
DpnII	GATC	6 cut(s)	86, 117, 298, 341, 527, 919
DraI	TTTAAA	1 cut(s)	211
DrdI	GACNNNNNNGTC	1 cut(s)	758
DriI	GACNNNNNGTC	1 cut(s)	1418
DseDI	GACNNNNNNGTC	1 cut(s)	758
EaeI	YGGCCR	2 cut(s)	485, 1010
Eam1104I	CTCTTC	2 cut(s)	600, 1051
Eam1105I	GACNNNNNGTC	1 cut(s)	1418
EarI	CTCTTC	2 cut(s)	600, 1051
Ecl136II	GAGCTC	1 cut(s)	43
Eco130I	CCWWGG	1 cut(s)	1096
Eco24I	GRGCYC	1 cut(s)	45
Eco31I	GGTCTC	1 cut(s)	744
Eco47I	GGWCC	2 cut(s)	240, 1412
Eco53kI	GAGCTC	1 cut(s)	43
Eco57I	CTGAAG	1 cut(s)	1032
EcoICRI	GAGCTC	1 cut(s)	43
EcoT14I	CCWWGG	1 cut(s)	1096
EcoT22I	ATGCAT	1 cut(s)	1195
EcoT38I	GRGCYC	1 cut(s)	45
ErhI	CCWWGG	1 cut(s)	1096
Esp3I	CGTCTC	1 cut(s)	1020
FalI	AAGNNNNNCTT	2 cut(s)	12, 44
Fnu4HI	GCNGC	5 cut(s)	261, 665, 954, 1143, 1446
FriOI	GRGCYC	1 cut(s)	45
Fsp4HI	GCNGC	5 cut(s)	261, 665, 954, 1143, 1446
FspBI	CTAG	4 cut(s)	668, 755, 1097, 1528
GlaI	GCGC	1 cut(s)	952
GluI	GCNGC	5 cut(s)	261, 665, 954, 1143, 1446
HaeII	RGCGCY	1 cut(s)	954
HaeIII	GGCC	5 cut(s)	5, 27, 487, 1012, 1231
HapII	CCGG	2 cut(s)	192, 296
HgaI	GACGC	1 cut(s)	1148
HhaI	GCGC	1 cut(s)	953
Hin6I	GCGC	1 cut(s)	951
HinP1I	GCGC	1 cut(s)	951
HincII	GTYRAC	1 cut(s)	1063
HindII	GTYRAC	1 cut(s)	1063
HindIII	AAGCTT	1 cut(s)	155
HinfI	GANTC	6 cut(s)	130, 716, 731, 816, 1064, 1516
HpaII	CCGG	2 cut(s)	192, 296
HphI	GGTGA	4 cut(s)	178, 683, 921, 1490
Hpy166II	GTNNAC	2 cut(s)	988, 1063
Hpy188I	TCNGA	3 cut(s)	401, 796, 972
Hpy188III	TCNNGA	3 cut(s)	187, 525, 892
Hpy8I	GTNNAC	2 cut(s)	988, 1063
HpyAV	CCTTC	2 cut(s)	367, 1066
HpyCH4III	ACNGT	3 cut(s)	275, 838, 898
HpyCH4IV	ACGT	1 cut(s)	454
HpyCH4V	TGCA	5 cut(s)	450, 652, 688, 847, 1193
HpyF10VI	GCNNNNNNNGC	2 cut(s)	447, 1009
HpyF3I	CTNAG	4 cut(s)	359, 390, 725, 1284
HpySE526I	ACGT	1 cut(s)	454
HspAI	GCGC	1 cut(s)	951
KpnI	GGTACC	1 cut(s)	77
Kzo9I	GATC	6 cut(s)	86, 117, 298, 341, 527, 919
LmnI	GCTCC	5 cut(s)	40, 292, 401, 541, 1352
Lsp1109I	GCAGC	3 cut(s)	676, 940, 1129
LweI	GCATC	5 cut(s)	697, 1169, 1180, 1265, 1439
MaeI	CTAG	4 cut(s)	668, 755, 1097, 1528
MaeII	ACGT	1 cut(s)	454
MaeIII	GTNAC	7 cut(s)	124, 636, 728, 912, 1102, 1147, 1499
MalI	GATC	6 cut(s)	88, 119, 300, 343, 529, 921
MboI	GATC	6 cut(s)	86, 117, 298, 341, 527, 919
MboII	GAAGA	8 cut(s)	390, 587, 632, 788, 1026, 1038, 1133, 1425
MhlI	GDGCHC	2 cut(s)	45, 831
MluCI	AATT	7 cut(s)	196, 206, 235, 326, 653, 822, 1261
MlyI	GAGTC	4 cut(s)	710, 725, 1058, 1510
MmeI	TCCRAC	1 cut(s)	547
MnlI	CCTC	6 cut(s)	354, 717, 933, 937, 953, 1246
Mph1103I	ATGCAT	1 cut(s)	1195
MseI	TTAA	9 cut(s)	30, 83, 159, 210, 543, 711, 1424, 1461, 1521
MslI	CAYNNNNRTG	2 cut(s)	1318, 1494
MspA1I	CMGCKG	1 cut(s)	358
MspCI	CTTAAG	1 cut(s)	710
MspI	CCGG	2 cut(s)	192, 296
Mva1269I	GAATGC	2 cut(s)	1193, 1285
MwoI	GCNNNNNNNGC	2 cut(s)	447, 1009
NdeII	GATC	6 cut(s)	86, 117, 298, 341, 527, 919
NlaIV	GGNNCC	3 cut(s)	75, 294, 1171
NmuCI	GTSAC	3 cut(s)	636, 728, 912
NsiI	ATGCAT	1 cut(s)	1195
NspI	RCATGY	3 cut(s)	450, 802, 1273
NspV	TTCGAA	2 cut(s)	57, 473
PaeI	GCATGC	1 cut(s)	450
PagI	TCATGA	1 cut(s)	186
PciI	ACATGT	2 cut(s)	798, 1269
PctI	GAATGC	2 cut(s)	1193, 1285
PfeI	GAWTC	2 cut(s)	130, 816
PflMI	CCANNNNNTGG	1 cut(s)	1463
PkrI	GCNGC	5 cut(s)	262, 666, 955, 1144, 1447
Ple19I	CGATCG	1 cut(s)	120
PleI	GAGTC	4 cut(s)	710, 725, 1058, 1510
PpsI	GAGTC	4 cut(s)	710, 725, 1058, 1510
PscI	ACATGT	2 cut(s)	798, 1269
Psp124BI	GAGCTC	1 cut(s)	45
PspN4I	GGNNCC	3 cut(s)	75, 294, 1171
PspPI	GGNCC	3 cut(s)	240, 1229, 1412
PvuI	CGATCG	1 cut(s)	120
PvuII	CAGCTG	1 cut(s)	358
RsaI	GTAC	4 cut(s)	75, 457, 494, 936
RsaNI	GTAC	4 cut(s)	74, 456, 493, 935
RseI	CAYNNNNRTG	2 cut(s)	1318, 1494
SacI	GAGCTC	1 cut(s)	45
SaqAI	TTAA	9 cut(s)	30, 83, 159, 210, 543, 711, 1424, 1461, 1521
SatI	GCNGC	5 cut(s)	261, 665, 954, 1143, 1446
Sau3AI	GATC	6 cut(s)	86, 117, 298, 341, 527, 919
Sau96I	GGNCC	3 cut(s)	240, 1229, 1412
SchI	GAGTC	4 cut(s)	710, 725, 1058, 1510
SduI	GDGCHC	2 cut(s)	45, 831
SfaNI	GCATC	5 cut(s)	697, 1169, 1180, 1265, 1439
SfcI	CTRYAG	2 cut(s)	274, 897
SfuI	TTCGAA	2 cut(s)	57, 473
SinI	GGWCC	2 cut(s)	240, 1412
SmiMI	CAYNNNNRTG	2 cut(s)	1318, 1494
SmlI	CTYRAG	3 cut(s)	710, 854, 996
SmoI	CTYRAG	3 cut(s)	710, 854, 996
SphI	GCATGC	1 cut(s)	450
Sse9I	AATT	7 cut(s)	196, 206, 235, 326, 653, 822, 1261
SsiI	CCGC	6 cut(s)	246, 261, 323, 412, 1341, 1445
SspMI	CTAG	4 cut(s)	668, 755, 1097, 1528
SstI	GAGCTC	1 cut(s)	45
StyI	CCWWGG	1 cut(s)	1096
TaaI	ACNGT	3 cut(s)	275, 838, 898
TaiI	ACGT	1 cut(s)	457
TaqI	TCGA	5 cut(s)	57, 116, 466, 473, 819
TasI	AATT	7 cut(s)	196, 206, 235, 326, 653, 822, 1261
TatI	WGTACW	1 cut(s)	934
TauI	GCSGC	2 cut(s)	263, 1448
TfiI	GAWTC	2 cut(s)	130, 816
Tru1I	TTAA	9 cut(s)	30, 83, 159, 210, 543, 711, 1424, 1461, 1521
Tru9I	TTAA	9 cut(s)	30, 83, 159, 210, 543, 711, 1424, 1461, 1521
TscAI	CASTG	2 cut(s)	645, 690
TseFI	GTSAC	3 cut(s)	636, 728, 912
TseI	GCWGC	3 cut(s)	664, 953, 1142
Tsp45I	GTSAC	3 cut(s)	636, 728, 912
TspRI	CASTG	2 cut(s)	645, 690
Van91I	CCANNNNNTGG	1 cut(s)	1463
Vha464I	CTTAAG	1 cut(s)	710
VpaK11BI	GGWCC	2 cut(s)	240, 1412
XapI	RAATTY	1 cut(s)	206
XceI	RCATGY	3 cut(s)	450, 802, 1273
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1026
XmaJI	CCTAGG	1 cut(s)	1096
XspI	CTAG	4 cut(s)	668, 755, 1097, 1528
Zsp2I	ATGCAT	1 cut(s)	1195

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.