Rh1AG439500

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003100
Physical Location & Seq
Reverse (-)
66228470 .. 66229201
732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG439500.1

Sequence Viewer

Length: 732 bp
ATGGCAGCATTGAGAGAGTTTTGTTCAAGATCAAACATGGAAGACAACCACAATCTGAGAGAGCTTTGTTTCAGATCAAACATGGGGGAAAACAATCTGAATGATAAGAAGGATAAGGTGGCTCAGGCTGCAGCCACCTTCGATCCTACAAAAAAGAAGAAAAAGAAGAAGGCAATTGCAATTGAGGAATCGAGTACTAGTACTGATGATTCCTCCATGAACAGCTCGACTTTGGAGAAGACAAAAACTGGCGAGGCTGACTACGAATATGAAGAACTTCTTGATAGGTTGCTGATGAAGCTTCGTGACAACAATCCGAAGCTTGCGAATGGAGAAAAGTGTGGTCTAGTTCTGAGTCCTCCTCAAGTTCTTCGTGAAGGGTCCAAAAAAACTGTTCTTGTCAATTTCATGGAGTACTGTAGGACTATGGTTAGGCAGCCGGATCACGTAATGAGTTTCATGCTTGCTGAATTGGGGACGAGTGGATCGCTTGATGGACAGCTGAGGTTAGTTGTGAAGGGAAGATTTTCGCCTAAGAATTTCGGAGCAATCTTGCGGAAATATGCCAATCAATATGTGATTTGCTTCGCGTGCAAACGTCCAGACACTCGACTTTCGAAAGAAAATCGGCTCAACTATCTTAGATGCGAGAGGTGTTGTGCTTCACGATCAGTTGCTCCTATTGAAGCTGGATTTCTTGCCAAAGTTGGAAAAAGAAACAGTGCGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

243

Amino Acids

27.36

Weight (kDa)

9.37

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-5_eIF-2B PF01873 117 - 221 2.1e-34 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 590
AciI CCGC 2 cut(s) 556, 725
AclWI GGATC 3 cut(s) 137, 450, 493
AcsI RAATTY 1 cut(s) 538
AfaI GTAC 3 cut(s) 196, 202, 416
AgsI TTSAA 2 cut(s) 27, 686
AhlI ACTAGT 1 cut(s) 197
AluBI AGCT 6 cut(s) 64, 225, 301, 322, 502, 689
AluI AGCT 6 cut(s) 64, 225, 301, 322, 502, 689
AlwI GGATC 3 cut(s) 137, 450, 493
ApeKI GCWGC 4 cut(s) 5, 128, 131, 436
ApoI RAATTY 1 cut(s) 538
Asp700I GAANNNNTTC 2 cut(s) 276, 526
AspS9I GGNCC 1 cut(s) 381
AsuII TTCGAA 1 cut(s) 617
AvaII GGWCC 1 cut(s) 381
BbsI GAAGAC 2 cut(s) 48, 245
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 4 cut(s) 17, 115, 143, 448
BccI CCATC 1 cut(s) 488
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 2 cut(s) 198, 347
BfmI CTRYAG 2 cut(s) 129, 418
BisI GCNGC 5 cut(s) 6, 129, 132, 437, 726
BlsI GCNGC 5 cut(s) 7, 130, 133, 438, 727
BmcAI AGTACT 3 cut(s) 196, 202, 416
Bme18I GGWCC 1 cut(s) 381
BmgT120I GGNCC 1 cut(s) 381
BmiI GGNNCC 1 cut(s) 382
BmsI GCATC 1 cut(s) 635
BpiI GAAGAC 2 cut(s) 48, 245
BplI GAGNNNNNCTC 2 cut(s) 346, 378
Bpu10I CCTNAGC 2 cut(s) 123, 503
Bpu14I TTCGAA 1 cut(s) 617
BpuEI CTTGAG 1 cut(s) 348
BsaAI YACGTR 1 cut(s) 448
Bse1I ACTGG 1 cut(s) 253
BseMII CTCAG 4 cut(s) 47, 137, 344, 494
BseNI ACTGG 1 cut(s) 253
BseRI GAGGAG 1 cut(s) 351
BseXI GCAGC 4 cut(s) 17, 115, 143, 448
Bsh1236I CGCG 1 cut(s) 590
BsiSI CCGG 1 cut(s) 440
BslFI GGGAC 1 cut(s) 490
BsmFI GGGAC 1 cut(s) 490
Bsp119I TTCGAA 1 cut(s) 617
Bsp143I GATC 6 cut(s) 29, 74, 142, 442, 485, 668
BspACI CCGC 2 cut(s) 556, 725
BspCNI CTCAG 4 cut(s) 48, 136, 345, 495
BspFNI CGCG 1 cut(s) 590
BspLI GGNNCC 1 cut(s) 382
BspMAI CTGCAG 1 cut(s) 133
BspPI GGATC 3 cut(s) 137, 450, 493
BspT104I TTCGAA 1 cut(s) 617
BsrI ACTGG 1 cut(s) 253
BssMI GATC 6 cut(s) 29, 74, 142, 442, 485, 668
Bst4CI ACNGT 3 cut(s) 394, 419, 722
BstBAI YACGTR 1 cut(s) 448
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 3 cut(s) 324, 465, 592
BstDEI CTNAG 6 cut(s) 56, 123, 353, 503, 534, 641
BstFNI CGCG 1 cut(s) 590
BstKTI GATC 6 cut(s) 32, 77, 145, 445, 488, 671
BstMBI GATC 6 cut(s) 29, 74, 142, 442, 485, 668
BstMWI GCNNNNNNNGC 3 cut(s) 128, 298, 591
BstSFI CTRYAG 2 cut(s) 129, 418
BstUI CGCG 1 cut(s) 590
BstV1I GCAGC 4 cut(s) 17, 115, 143, 448
BstV2I GAAGAC 2 cut(s) 48, 245
BtsIMutI CAGTG 1 cut(s) 727
Cac8I GCNNGC 3 cut(s) 324, 465, 592
Cfr13I GGNCC 1 cut(s) 381
Csp6I GTAC 3 cut(s) 195, 201, 415
CviAII CATG 6 cut(s) 37, 82, 217, 409, 460, 729
CviQI GTAC 3 cut(s) 195, 201, 415
DdeI CTNAG 6 cut(s) 56, 123, 353, 503, 534, 641
DpnI GATC 6 cut(s) 31, 76, 144, 444, 487, 670
DpnII GATC 6 cut(s) 29, 74, 142, 442, 485, 668
Eco47I GGWCC 1 cut(s) 381
FaeI CATG 6 cut(s) 40, 85, 220, 412, 463, 732
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FaqI GGGAC 1 cut(s) 490
FatI CATG 6 cut(s) 36, 81, 216, 408, 459, 728
Fnu4HI GCNGC 5 cut(s) 6, 129, 132, 437, 726
Fsp4HI GCNGC 5 cut(s) 6, 129, 132, 437, 726
FspBI CTAG 2 cut(s) 198, 347
GluI GCNGC 5 cut(s) 6, 129, 132, 437, 726
HapII CCGG 1 cut(s) 440
Hin1II CATG 6 cut(s) 40, 85, 220, 412, 463, 732
HindIII AAGCTT 2 cut(s) 299, 320
HinfI GANTC 3 cut(s) 188, 209, 355
HpaII CCGG 1 cut(s) 440
Hpy188I TCNGA 6 cut(s) 57, 74, 99, 318, 354, 545
Hpy188III TCNNGA 6 cut(s) 27, 281, 305, 374, 602, 666
HpyAV CCTTC 5 cut(s) 103, 148, 163, 371, 511
HpyCH4III ACNGT 3 cut(s) 394, 419, 722
HpyCH4IV ACGT 2 cut(s) 447, 598
HpyCH4V TGCA 3 cut(s) 131, 179, 594
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 298, 591
HpyF3I CTNAG 6 cut(s) 56, 123, 353, 503, 534, 641
HpySE526I ACGT 2 cut(s) 447, 598
Hsp92II CATG 6 cut(s) 40, 85, 220, 412, 463, 732
Kzo9I GATC 6 cut(s) 29, 74, 142, 442, 485, 668
LmnI GCTCC 2 cut(s) 545, 682
LpnPI CCDG 5 cut(s) 110, 234, 453, 615, 675
Lsp1109I GCAGC 4 cut(s) 17, 115, 143, 448
LweI GCATC 1 cut(s) 635
MaeI CTAG 2 cut(s) 198, 347
MaeII ACGT 2 cut(s) 447, 598
MaeIII GTNAC 1 cut(s) 305
MalI GATC 6 cut(s) 31, 76, 144, 444, 487, 670
MboI GATC 6 cut(s) 29, 74, 142, 442, 485, 668
MboII GAAGA 7 cut(s) 53, 169, 178, 250, 284, 362, 534
MfeI CAATTG 2 cut(s) 174, 180
MluCI AATT 5 cut(s) 174, 180, 403, 470, 538
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 1 cut(s) 688
MnlI CCTC 7 cut(s) 178, 223, 247, 369, 372, 498, 645
MroXI GAANNNNTTC 2 cut(s) 276, 526
MspA1I CMGCKG 1 cut(s) 502
MspI CCGG 1 cut(s) 440
MunI CAATTG 2 cut(s) 174, 180
MvnI CGCG 1 cut(s) 590
MwoI GCNNNNNNNGC 3 cut(s) 128, 298, 591
NdeII GATC 6 cut(s) 29, 74, 142, 442, 485, 668
NlaIII CATG 6 cut(s) 40, 85, 220, 412, 463, 732
NlaIV GGNNCC 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 305
NspV TTCGAA 1 cut(s) 617
PdmI GAANNNNTTC 2 cut(s) 276, 526
PfeI GAWTC 2 cut(s) 188, 209
PkrI GCNGC 5 cut(s) 7, 130, 133, 438, 727
PleI GAGTC 1 cut(s) 363
PpsI GAGTC 1 cut(s) 363
Ppu21I YACGTR 1 cut(s) 448
PspN4I GGNNCC 1 cut(s) 382
PspPI GGNCC 1 cut(s) 381
PstI CTGCAG 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 502
RsaI GTAC 3 cut(s) 196, 202, 416
RsaNI GTAC 3 cut(s) 195, 201, 415
SatI GCNGC 5 cut(s) 6, 129, 132, 437, 726
Sau3AI GATC 6 cut(s) 29, 74, 142, 442, 485, 668
Sau96I GGNCC 1 cut(s) 381
ScaI AGTACT 3 cut(s) 196, 202, 416
SchI GAGTC 1 cut(s) 364
SfaNI GCATC 1 cut(s) 635
SfcI CTRYAG 2 cut(s) 129, 418
SfuI TTCGAA 1 cut(s) 617
SinI GGWCC 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 363
SmoI CTYRAG 1 cut(s) 363
SpeI ACTAGT 1 cut(s) 197
Sse9I AATT 5 cut(s) 174, 180, 403, 470, 538
SsiI CCGC 2 cut(s) 556, 725
SspMI CTAG 2 cut(s) 198, 347
TaaI ACNGT 3 cut(s) 394, 419, 722
TaiI ACGT 2 cut(s) 450, 601
TaqI TCGA 5 cut(s) 141, 191, 227, 610, 617
TasI AATT 5 cut(s) 174, 180, 403, 470, 538
TatI WGTACW 3 cut(s) 194, 200, 414
TauI GCSGC 1 cut(s) 728
TfiI GAWTC 2 cut(s) 188, 209
TscAI CASTG 1 cut(s) 727
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 4 cut(s) 5, 128, 131, 436
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 5 cut(s) 233, 285, 311, 397, 448
TspRI CASTG 1 cut(s) 727
VpaK11BI GGWCC 1 cut(s) 381
XapI RAATTY 1 cut(s) 538
XmnI GAANNNNTTC 2 cut(s) 276, 526
XspI CTAG 2 cut(s) 198, 347
ZrmI AGTACT 3 cut(s) 196, 202, 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.