pycom03g03960

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003100
Physical Location & Seq
Forward (+)
3137539 .. 3139177
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03960.2

Sequence Viewer

Length: 381 bp
ATGAGGCCTCCACAAGTTCTTCGTGAGGGCACAAAGAAAACTGTTTTTGTGAATTTCATGGATCTATGCAAGACGATGCATAGGCAGCCAGATCATGTCATGGCATTCTTGCTTGCCGAACTGGGAACAAGTGGATCACTTGATGGGCAGCAGAGGTTGGTTGTTAAGGGAAGATTTGCACCAAAGAACTTTGAAGGAATACTGCGGCGATATGTCAATGAATATGTCATTTGCCTTGGGTGCAAAAGTCCAGACACTATACTTTCAAAGGAGAATCGCCTCTTCTTTCTCAGATGCGAGAAGTGTGGTTCTGGACGATCTGTCGCTCCAATTAAAGCTGGTTTTATGGCTCGTGTTGGGAGAAGGAATGCCGGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

127

Amino Acids

14.17

Weight (kDa)

10.11

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 205
AclWI GGATC 2 cut(s) 69, 142
AcsI RAATTY 1 cut(s) 52
AgsI TTSAA 2 cut(s) 194, 267
AhdI GACNNNNNGTC 1 cut(s) 320
AluBI AGCT 1 cut(s) 338
AluI AGCT 1 cut(s) 338
AlwI GGATC 2 cut(s) 69, 142
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 2 cut(s) 85, 148
ApoI RAATTY 1 cut(s) 52
BaeGI GKGCMC 1 cut(s) 32
BauI CACGAG 1 cut(s) 351
BbvI GCAGC 2 cut(s) 97, 160
BccI CCATC 1 cut(s) 137
BisI GCNGC 3 cut(s) 86, 149, 206
BlsI GCNGC 3 cut(s) 87, 150, 207
BmeRI GACNNNNNGTC 1 cut(s) 320
BmrI ACTGGG 1 cut(s) 131
BmsI GCATC 2 cut(s) 66, 284
BmuI ACTGGG 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 235
BseMII CTCAG 1 cut(s) 304
BseNI ACTGG 1 cut(s) 126
BseSI GKGCMC 1 cut(s) 32
BseXI GCAGC 2 cut(s) 97, 160
BshFI GGCC 1 cut(s) 7
BsiSI CCGG 1 cut(s) 372
BsmI GAATGC 2 cut(s) 104, 373
BsnI GGCC 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 4 cut(s) 61, 91, 134, 317
BspACI CCGC 1 cut(s) 205
BspANI GGCC 1 cut(s) 7
BspCNI CTCAG 1 cut(s) 303
BspPI GGATC 2 cut(s) 69, 142
BsrI ACTGG 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 4 cut(s) 61, 91, 134, 317
BssSI CACGAG 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 235
Bst2BI CACGAG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 43
Bst6I CTCTTC 1 cut(s) 287
BstC8I GCNNGC 1 cut(s) 114
BstDEI CTNAG 1 cut(s) 290
BstKTI GATC 4 cut(s) 64, 94, 137, 320
BstMBI GATC 4 cut(s) 61, 91, 134, 317
BstMWI GCNNNNNNNGC 2 cut(s) 85, 240
BstSLI GKGCMC 1 cut(s) 32
BstV1I GCAGC 2 cut(s) 97, 160
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
BsuRI GGCC 1 cut(s) 7
Cac8I GCNNGC 1 cut(s) 114
CviAII CATG 3 cut(s) 58, 95, 100
CviJI RGCY 4 cut(s) 7, 88, 338, 350
CviKI_1 RGCY 4 cut(s) 7, 88, 338, 350
DdeI CTNAG 1 cut(s) 290
DpnI GATC 4 cut(s) 63, 93, 136, 319
DpnII GATC 4 cut(s) 61, 91, 134, 317
DriI GACNNNNNGTC 1 cut(s) 320
Eam1104I CTCTTC 1 cut(s) 287
Eam1105I GACNNNNNGTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 287
Eco130I CCWWGG 1 cut(s) 235
Eco147I AGGCCT 1 cut(s) 7
EcoT14I CCWWGG 1 cut(s) 235
EcoT22I ATGCAT 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 3 cut(s) 61, 98, 103
FatI CATG 3 cut(s) 57, 94, 99
Fnu4HI GCNGC 3 cut(s) 86, 149, 206
Fsp4HI GCNGC 3 cut(s) 86, 149, 206
GluI GCNGC 3 cut(s) 86, 149, 206
HaeIII GGCC 1 cut(s) 7
HapII CCGG 1 cut(s) 372
Hin1II CATG 3 cut(s) 61, 98, 103
HinfI GANTC 1 cut(s) 274
HpaII CCGG 1 cut(s) 372
Hpy188I TCNGA 1 cut(s) 293
Hpy188III TCNNGA 3 cut(s) 23, 251, 312
HpyAV CCTTC 2 cut(s) 188, 357
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4V TGCA 4 cut(s) 69, 79, 179, 243
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 240
HpyF3I CTNAG 1 cut(s) 290
Hsp92II CATG 3 cut(s) 61, 98, 103
Kzo9I GATC 4 cut(s) 61, 91, 134, 317
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 5 cut(s) 102, 107, 264, 297, 324
Lsp1109I GCAGC 2 cut(s) 97, 160
LweI GCATC 2 cut(s) 66, 284
MalI GATC 4 cut(s) 63, 93, 136, 319
MboI GATC 4 cut(s) 61, 91, 134, 317
MboII GAAGA 3 cut(s) 11, 183, 274
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 2 cut(s) 52, 330
MnlI CCTC 4 cut(s) 18, 19, 147, 290
Mph1103I ATGCAT 1 cut(s) 81
MseI TTAA 2 cut(s) 165, 333
MspI CCGG 1 cut(s) 372
Mva1269I GAATGC 2 cut(s) 104, 373
MwoI GCNNNNNNNGC 2 cut(s) 85, 240
NdeII GATC 4 cut(s) 61, 91, 134, 317
NlaIII CATG 3 cut(s) 61, 98, 103
NsiI ATGCAT 1 cut(s) 81
PceI AGGCCT 1 cut(s) 7
PctI GAATGC 2 cut(s) 104, 373
PfeI GAWTC 1 cut(s) 274
PkrI GCNGC 3 cut(s) 87, 150, 207
PsuI RGATCY 1 cut(s) 61
SaqAI TTAA 2 cut(s) 165, 333
SatI GCNGC 3 cut(s) 86, 149, 206
Sau3AI GATC 4 cut(s) 61, 91, 134, 317
SduI GDGCHC 1 cut(s) 32
SetI ASST 2 cut(s) 158, 340
SfaNI GCATC 2 cut(s) 66, 284
Sse9I AATT 2 cut(s) 52, 330
SseBI AGGCCT 1 cut(s) 7
SsiI CCGC 1 cut(s) 205
StuI AGGCCT 1 cut(s) 7
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 1 cut(s) 43
TasI AATT 2 cut(s) 52, 330
TauI GCSGC 1 cut(s) 208
TfiI GAWTC 1 cut(s) 274
Tru1I TTAA 2 cut(s) 165, 333
Tru9I TTAA 2 cut(s) 165, 333
TseI GCWGC 2 cut(s) 85, 148
TspDTI ATGAA 2 cut(s) 46, 234
XapI RAATTY 1 cut(s) 52
Zsp2I ATGCAT 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.