Rroxscaffold_4G00279880

Bacterial Ig-like domain 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003100
Physical Location & Seq
Forward (+)
2533185 .. 2534822
1638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00279880.1

Sequence Viewer

Length: 1071 bp
ATGTACCTTGTCCCTGGAACGCATCTGGATGTCATGCTTCTTGGTGGTCTCGAACAGTGGAAAAATGGTGTTGAGTTTGTGGAAACTGTGGAGATCTTAAATAAAGAACATGGTCACATTGATGATGGTGCTTGTGTGAAACGGTTATCTGAGACCTATAGGAGTCTGTACAGAGTTTCACGTGAGATGCTTGGAACCTATAGTATTGTTCGTGGTAATCTTGTTGGGGATGACCATCCTATGCCTGCAGTGGCTGACGTGTCGTTGTCTCTTATATGTAGCATTCCTACCTCAATTGCTTTGATAGCTGATGAACCCGTAATTCAACTTGAAGTTATAAGAACAGCAATCCAGGCTGACCGTAGTTCAGGGAGAATTCGTGTCACTCCCATTACTGTGGCTAATGAGCGCACCATTAGATTAGCTGCAGTTGGCATCACTAGTAATGGAGAAGCTTTTGCAAACTCATCATCTCTTCATTTTCAGTGGGAACTTAGCAGTTGTGATGGGATGGCCTATTGGAATGATGCTGATAATTTACAGAGATCAAAGTACAGTTGGGAAAAAATTTGGAGCCTGCAAAATGTGTCGCGCGCGCCTGTCAAAGCAGAGGCAGAAACATGCAGGGAGGAAAAGATCACAGAGATGGCAGCATTGAGAGAGTTCTGTTCAAGATCAAACATGGAAGACAACCACAATCGATTGAGAGAGCTTTGTTTCAGATCAAACATGGGGGAAAACAATCTGAATGATAAGAAGGATAAGGTGGCTCAGGCTGCAGCCACCTTCGATCCTACAAAAAAGAAGAAGAAGAAGAAGGCAATTGCAATTGAGGAATCGAGTAATAGTACTGATGATTCCTCCATGAACAGCTCGGCTTTAGAGAAGACAAAAACTGGCGAGGCTGACTACGAATATGAAGAACTTCTTGATAGGTTGCTGATGAAGCTTCGTGACGACAATCCGAAGCTTGCGGATGGAGAAAAGTGTGGTCTAGTTCTGAGTCCTCCTCAAGTTCTTCGTGAAGGGTCCAGAAAAAACTGTTCTTGTCAATTTCATGGAGTACTGTAG

Protein Analysis

356

Amino Acids

39.79

Weight (kDa)

5.66

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_NUP210_7th PF22962 2 - 94 1.9e-07 NUP210 Ig-like domain 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 338
AccII CGCG 3 cut(s) 592, 594, 596
AciI CCGC 1 cut(s) 974
AclWI GGATC 1 cut(s) 785
AcsI RAATTY 2 cut(s) 375, 567
AcvI CACGTG 1 cut(s) 182
AfaI GTAC 5 cut(s) 5, 170, 554, 850, 1065
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 3 cut(s) 326, 332, 672
AhlI ACTAGT 1 cut(s) 440
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 2 cut(s) 13, 351
AluBI AGCT 7 cut(s) 308, 425, 455, 712, 873, 949, 970
AluI AGCT 7 cut(s) 308, 425, 455, 712, 873, 949, 970
Alw26I GTCTC 3 cut(s) 53, 146, 273
AlwI GGATC 1 cut(s) 785
AlwNI CAGNNNCTG 1 cut(s) 254
AoxI GGCC 1 cut(s) 513
ApeKI GCWGC 4 cut(s) 425, 650, 776, 779
ApoI RAATTY 2 cut(s) 375, 567
Asp700I GAANNNNTTC 1 cut(s) 924
AspLEI GCGC 4 cut(s) 411, 594, 596, 598
AspS9I GGNCC 1 cut(s) 1029
AvaII GGWCC 1 cut(s) 1029
BbrPI CACGTG 1 cut(s) 182
BbsI GAAGAC 2 cut(s) 693, 893
BbvI GCAGC 4 cut(s) 412, 662, 763, 791
BccI CCATC 6 cut(s) 119, 243, 500, 505, 640, 971
BciT130I CCWGG 2 cut(s) 15, 353
BcoDI GTCTC 3 cut(s) 53, 146, 273
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 2 cut(s) 441, 995
BfmI CTRYAG 6 cut(s) 157, 199, 246, 426, 777, 1067
BglII AGATCT 1 cut(s) 93
BisI GCNGC 4 cut(s) 426, 651, 777, 780
BlsI GCNGC 4 cut(s) 427, 652, 778, 781
BmcAI AGTACT 2 cut(s) 850, 1065
Bme1390I CCNGG 2 cut(s) 15, 353
Bme18I GGWCC 1 cut(s) 1029
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 1029
BmiI GGNNCC 3 cut(s) 196, 575, 1030
BmrFI CCNGG 2 cut(s) 15, 353
BmsI GCATC 4 cut(s) 31, 177, 444, 517
BpiI GAAGAC 2 cut(s) 693, 893
BplI GAGNNNNNCTC 2 cut(s) 994, 1026
Bpu10I CCTNAGC 1 cut(s) 771
BpuEI CTTGAG 1 cut(s) 996
Bsa29I ATCGAT 1 cut(s) 700
BsaAI YACGTR 1 cut(s) 182
BsaBI GATNNNNATC 1 cut(s) 234
BsaI GGTCTC 2 cut(s) 53, 146
BsaJI CCNNGG 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 364, 394
Bse1I ACTGG 1 cut(s) 901
Bse8I GATNNNNATC 1 cut(s) 234
BseBI CCWGG 2 cut(s) 15, 353
BseCI ATCGAT 1 cut(s) 700
BseDI CCNNGG 1 cut(s) 13
BseGI GGATG 5 cut(s) 34, 235, 235, 516, 982
BseJI GATNNNNATC 1 cut(s) 234
BseMII CTCAG 3 cut(s) 141, 785, 992
BseNI ACTGG 1 cut(s) 901
BsePI GCGCGC 2 cut(s) 592, 594
BseRI GAGGAG 1 cut(s) 999
BseXI GCAGC 4 cut(s) 412, 662, 763, 791
Bsh1236I CGCG 3 cut(s) 592, 594, 596
BshFI GGCC 1 cut(s) 515
BshVI ATCGAT 1 cut(s) 700
BsmAI GTCTC 3 cut(s) 53, 146, 273
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 1 cut(s) 515
Bso31I GGTCTC 2 cut(s) 53, 146
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 6 cut(s) 93, 545, 636, 674, 722, 790
BspACI CCGC 1 cut(s) 974
BspANI GGCC 1 cut(s) 515
BspCNI CTCAG 3 cut(s) 142, 784, 993
BspDI ATCGAT 1 cut(s) 700
BspFNI CGCG 3 cut(s) 592, 594, 596
BspLI GGNNCC 3 cut(s) 196, 575, 1030
BspMAI CTGCAG 3 cut(s) 250, 430, 781
BspPI GGATC 1 cut(s) 785
BspTNI GGTCTC 2 cut(s) 53, 146
BsrGI TGTACA 1 cut(s) 168
BsrI ACTGG 1 cut(s) 901
BssECI CCNNGG 1 cut(s) 13
BssHII GCGCGC 2 cut(s) 592, 594
BssMI GATC 6 cut(s) 93, 545, 636, 674, 722, 790
Bst2UI CCWGG 2 cut(s) 15, 353
Bst4CI ACNGT 8 cut(s) 57, 88, 144, 362, 397, 557, 1043, 1068
Bst6I CTCTTC 1 cut(s) 480
BstAUI TGTACA 1 cut(s) 168
BstBAI YACGTR 1 cut(s) 182
BstC8I GCNNGC 5 cut(s) 246, 578, 594, 596, 972
BstDEI CTNAG 4 cut(s) 150, 494, 771, 1001
BstF5I GGATG 5 cut(s) 34, 235, 235, 516, 982
BstFNI CGCG 3 cut(s) 592, 594, 596
BstHHI GCGC 4 cut(s) 411, 594, 596, 598
BstKTI GATC 6 cut(s) 96, 548, 639, 677, 725, 793
BstMAI GTCTC 3 cut(s) 53, 146, 273
BstMBI GATC 6 cut(s) 93, 545, 636, 674, 722, 790
BstMWI GCNNNNNNNGC 4 cut(s) 305, 353, 776, 946
BstNI CCWGG 2 cut(s) 15, 353
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 2 cut(s) 13, 351
BstSFI CTRYAG 6 cut(s) 157, 199, 246, 426, 777, 1067
BstUI CGCG 3 cut(s) 592, 594, 596
BstV1I GCAGC 4 cut(s) 412, 662, 763, 791
BstV2I GAAGAC 2 cut(s) 693, 893
BstX2I RGATCY 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 397
BstYI RGATCY 1 cut(s) 93
Bsu15I ATCGAT 1 cut(s) 700
BsuRI GGCC 1 cut(s) 515
BsuTUI ATCGAT 1 cut(s) 700
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 5 cut(s) 34, 235, 235, 516, 982
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 3 cut(s) 62, 255, 491
Cac8I GCNNGC 5 cut(s) 246, 578, 594, 596, 972
CaiI CAGNNNCTG 1 cut(s) 254
CfoI GCGC 4 cut(s) 411, 594, 596, 598
Cfr13I GGNCC 1 cut(s) 1029
ClaI ATCGAT 1 cut(s) 700
Csp6I GTAC 5 cut(s) 4, 169, 553, 849, 1064
CviAII CATG 7 cut(s) 34, 110, 621, 682, 730, 865, 1058
CviQI GTAC 5 cut(s) 4, 169, 553, 849, 1064
DdeI CTNAG 4 cut(s) 150, 494, 771, 1001
DpnI GATC 6 cut(s) 95, 547, 638, 676, 724, 792
DpnII GATC 6 cut(s) 93, 545, 636, 674, 722, 790
Eam1104I CTCTTC 1 cut(s) 480
EarI CTCTTC 1 cut(s) 480
Eco31I GGTCTC 2 cut(s) 53, 146
Eco47I GGWCC 1 cut(s) 1029
Eco72I CACGTG 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 375
EcoRII CCWGG 2 cut(s) 13, 351
FaeI CATG 7 cut(s) 37, 113, 624, 685, 733, 868, 1061
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FatI CATG 7 cut(s) 33, 109, 620, 681, 729, 864, 1057
Fnu4HI GCNGC 4 cut(s) 426, 651, 777, 780
FokI GGATG 5 cut(s) 41, 222, 242, 523, 989
Fsp4HI GCNGC 4 cut(s) 426, 651, 777, 780
FspBI CTAG 2 cut(s) 441, 995
GlaI GCGC 4 cut(s) 410, 593, 595, 597
GluI GCNGC 4 cut(s) 426, 651, 777, 780
HaeIII GGCC 1 cut(s) 515
HhaI GCGC 4 cut(s) 411, 594, 596, 598
Hin1II CATG 7 cut(s) 37, 113, 624, 685, 733, 868, 1061
Hin6I GCGC 4 cut(s) 409, 592, 594, 596
HinP1I GCGC 4 cut(s) 409, 592, 594, 596
HindIII AAGCTT 3 cut(s) 453, 947, 968
HinfI GANTC 4 cut(s) 163, 836, 857, 1003
Hpy188I TCNGA 5 cut(s) 151, 722, 747, 966, 1002
Hpy188III TCNNGA 7 cut(s) 26, 50, 672, 929, 953, 1022, 1032
HpyAV CCTTC 4 cut(s) 751, 796, 811, 1019
HpyCH4III ACNGT 8 cut(s) 57, 88, 144, 362, 397, 557, 1043, 1068
HpyCH4IV ACGT 2 cut(s) 181, 258
HpyCH4V TGCA 7 cut(s) 248, 428, 461, 580, 624, 779, 827
HpyF10VI GCNNNNNNNGC 4 cut(s) 305, 353, 776, 946
HpyF3I CTNAG 4 cut(s) 150, 494, 771, 1001
HpySE526I ACGT 2 cut(s) 181, 258
Hsp92II CATG 7 cut(s) 37, 113, 624, 685, 733, 868, 1061
HspAI GCGC 4 cut(s) 409, 592, 594, 596
Kzo9I GATC 6 cut(s) 93, 545, 636, 674, 722, 790
LmnI GCTCC 1 cut(s) 573
Lsp1109I GCAGC 4 cut(s) 412, 662, 763, 791
LweI GCATC 4 cut(s) 31, 177, 444, 517
MaeI CTAG 2 cut(s) 441, 995
MaeII ACGT 2 cut(s) 181, 258
MaeIII GTNAC 3 cut(s) 113, 382, 953
MalI GATC 6 cut(s) 95, 547, 638, 676, 724, 792
MauBI CGCGCGCG 1 cut(s) 592
MboI GATC 6 cut(s) 93, 545, 636, 674, 722, 790
MboII GAAGA 9 cut(s) 467, 698, 817, 820, 823, 826, 898, 932, 1010
MfeI CAATTG 3 cut(s) 294, 822, 828
MflI RGATCY 1 cut(s) 93
MluCI AATT 8 cut(s) 294, 321, 375, 535, 567, 822, 828, 1052
MlyI GAGTC 2 cut(s) 172, 1012
MnlI CCTC 8 cut(s) 301, 604, 622, 826, 871, 895, 1017, 1020
MroXI GAANNNNTTC 1 cut(s) 924
MseI TTAA 1 cut(s) 98
MslI CAYNNNNRTG 4 cut(s) 27, 120, 395, 644
MspR9I CCNGG 2 cut(s) 15, 353
MunI CAATTG 3 cut(s) 294, 822, 828
Mva1269I GAATGC 1 cut(s) 282
MvaI CCWGG 2 cut(s) 15, 353
MvnI CGCG 3 cut(s) 592, 594, 596
MwoI GCNNNNNNNGC 4 cut(s) 305, 353, 776, 946
NdeII GATC 6 cut(s) 93, 545, 636, 674, 722, 790
NlaIII CATG 7 cut(s) 37, 113, 624, 685, 733, 868, 1061
NlaIV GGNNCC 3 cut(s) 196, 575, 1030
NmeAIII GCCGAG 1 cut(s) 854
NmuCI GTSAC 3 cut(s) 113, 382, 953
NspI RCATGY 1 cut(s) 624
PauI GCGCGC 2 cut(s) 592, 594
PctI GAATGC 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 924
PfeI GAWTC 2 cut(s) 836, 857
PkrI GCNGC 4 cut(s) 427, 652, 778, 781
PleI GAGTC 2 cut(s) 171, 1011
PmaCI CACGTG 1 cut(s) 182
PmlI CACGTG 1 cut(s) 182
PpsI GAGTC 2 cut(s) 171, 1011
Ppu21I YACGTR 1 cut(s) 182
PsiI TTATAA 1 cut(s) 338
Psp6I CCWGG 2 cut(s) 13, 351
PspCI CACGTG 1 cut(s) 182
PspGI CCWGG 2 cut(s) 13, 351
PspN4I GGNNCC 3 cut(s) 196, 575, 1030
PspPI GGNCC 1 cut(s) 1029
PstI CTGCAG 3 cut(s) 250, 430, 781
PstNI CAGNNNCTG 1 cut(s) 254
PsuI RGATCY 1 cut(s) 93
PteI GCGCGC 2 cut(s) 592, 594
RsaI GTAC 5 cut(s) 5, 170, 554, 850, 1065
RsaNI GTAC 5 cut(s) 4, 169, 553, 849, 1064
RseI CAYNNNNRTG 4 cut(s) 27, 120, 395, 644
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 4 cut(s) 426, 651, 777, 780
Sau3AI GATC 6 cut(s) 93, 545, 636, 674, 722, 790
Sau96I GGNCC 1 cut(s) 1029
ScaI AGTACT 2 cut(s) 850, 1065
SchI GAGTC 2 cut(s) 172, 1012
ScrFI CCNGG 2 cut(s) 15, 353
SfaNI GCATC 4 cut(s) 31, 177, 444, 517
SfcI CTRYAG 6 cut(s) 157, 199, 246, 426, 777, 1067
SinI GGWCC 1 cut(s) 1029
SmiMI CAYNNNNRTG 4 cut(s) 27, 120, 395, 644
SmlI CTYRAG 1 cut(s) 1011
SmoI CTYRAG 1 cut(s) 1011
SpeI ACTAGT 1 cut(s) 440
Sse9I AATT 8 cut(s) 294, 321, 375, 535, 567, 822, 828, 1052
SsiI CCGC 1 cut(s) 974
SspMI CTAG 2 cut(s) 441, 995
StyD4I CCNGG 2 cut(s) 13, 351
TaaI ACNGT 8 cut(s) 57, 88, 144, 362, 397, 557, 1043, 1068
TaiI ACGT 2 cut(s) 184, 261
TaqI TCGA 4 cut(s) 51, 700, 789, 839
TasI AATT 8 cut(s) 294, 321, 375, 535, 567, 822, 828, 1052
TatI WGTACW 4 cut(s) 168, 552, 848, 1063
TfiI GAWTC 2 cut(s) 836, 857
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TscAI CASTG 3 cut(s) 62, 255, 491
TseFI GTSAC 3 cut(s) 113, 382, 953
TseI GCWGC 4 cut(s) 425, 650, 776, 779
Tsp45I GTSAC 3 cut(s) 113, 382, 953
TspDTI ATGAA 6 cut(s) 327, 467, 881, 933, 959, 1046
TspRI CASTG 3 cut(s) 62, 255, 491
VpaK11BI GGWCC 1 cut(s) 1029
XapI RAATTY 2 cut(s) 375, 567
XceI RCATGY 1 cut(s) 624
XmnI GAANNNNTTC 1 cut(s) 924
XspI CTAG 2 cut(s) 441, 995
ZrmI AGTACT 2 cut(s) 850, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.