MD03G1050000.v1.1

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003100
Physical Location & Seq
Forward (+)
4001411 .. 4005189
3779 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1050000.v1.1.491

Sequence Viewer

Length: 816 bp
ATGGCGGACGACAATCAGAATGAGGTGAAGGACGAGGTGGTAGCAGATATTGCCCCCTTTGATCCTACTAAGAAGAAGAAAAAGAAGAAGGTTGTTATTCAGGATACTACTGATGATTCTGTGGAAAAGTTGGCTGAGAAAGCGGAAAGCTTGACAGTTTCTGAGGGGCAGGAGAGTACGTTTGCTGGTTTGAAAAAGAAGAAGAAGAAGCCAATTGAGACTAGTATCTTGAATGAAGAGAGTGGCGATGCAGTGGAAGATTTAAATGAGCGCACTGGTGAGGATGAGGAAGGAGAAGGGATTGTTCTAGAGACTCCATCATATCCTTGGGAGGGGAGTGATCGCGACTACACTTATGAAGAGCTTCTTGATAGGGTGTTTAACATACTTCGCGAAAATAACCCGGATCTGGCTGGAGACAGGCGTAGGACAGTGATGAGGCCTCCACAAGTTCTTCGTGAGGGCACAAAGAAAACTGTTTTTGTGAATTTCATGGATCTATGCAAGACGATGCATAGGCAGCCGGATCACGTCATGGCATTCTTGCTTGCCGAACTGGGAACAAGTGGATCACTTGATGGACAGCAGAGGTTGGTTGTTAAGGGAAGATTTGCACCAAAGAATTTTGAAGGAATACTGCGGCGATATGTCAATGAATATGTCATTTGCCTTGGGTGCAAAAGTCCAGACACTATACTGTCAAAGGAGAATCGTCTCTTCTTTCTCAGATGCGAGAAGTGTGGTTCTGGACGATCTGTCGCTCCAATTAAAGCTGGTTTTATGGCTCGTGTTGGGAGAAGGAATGCCGGAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

272

Amino Acids

30.51

Weight (kDa)

5.96

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-5_eIF-2B PF01873 140 - 249 1.1e-39 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 345, 393
AciI CCGC 3 cut(s) 5, 143, 640
AclWI GGATC 5 cut(s) 56, 414, 504, 534, 577
AcsI RAATTY 2 cut(s) 487, 622
AfaI GTAC 1 cut(s) 178
AfiI CCNNNNNNNGG 2 cut(s) 332, 409
AgsI TTSAA 3 cut(s) 193, 232, 629
AhdI GACNNNNNGTC 1 cut(s) 755
AhlI ACTAGT 1 cut(s) 221
AjiI CACGTC 1 cut(s) 532
AluBI AGCT 3 cut(s) 150, 364, 773
AluI AGCT 3 cut(s) 150, 364, 773
Alw26I GTCTC 4 cut(s) 212, 305, 411, 719
AlwI GGATC 5 cut(s) 56, 414, 504, 534, 577
AlwNI CAGNNNCTG 1 cut(s) 161
AoxI GGCC 1 cut(s) 440
ApeKI GCWGC 1 cut(s) 520
ApoI RAATTY 2 cut(s) 487, 622
Asp700I GAANNNNTTC 1 cut(s) 363
AspLEI GCGC 1 cut(s) 273
AsuC2I CCSGG 1 cut(s) 404
AsuHPI GGTGA 2 cut(s) 37, 290
BaeGI GKGCMC 1 cut(s) 467
BauI CACGAG 1 cut(s) 786
BbvI GCAGC 1 cut(s) 532
BccI CCATC 2 cut(s) 325, 572
BciVI GTATCC 1 cut(s) 97
BcnI CCSGG 1 cut(s) 404
BcoDI GTCTC 4 cut(s) 212, 305, 411, 719
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 2 cut(s) 222, 308
BfuI GTATCC 1 cut(s) 97
BisI GCNGC 2 cut(s) 521, 641
BlsI GCNGC 2 cut(s) 522, 642
Bme1390I CCNGG 1 cut(s) 404
BmeRI GACNNNNNGTC 1 cut(s) 755
BmgBI CACGTC 1 cut(s) 532
BmiI GGNNCC 1 cut(s) 811
BmrFI CCNGG 1 cut(s) 404
BmrI ACTGGG 1 cut(s) 566
BmsI GCATC 3 cut(s) 238, 501, 719
BmuI ACTGGG 1 cut(s) 566
BpmI CTGGAG 1 cut(s) 435
BpuMI CCSGG 1 cut(s) 404
BsaJI CCNNGG 2 cut(s) 326, 670
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 409
Bse1I ACTGG 2 cut(s) 280, 561
BseDI CCNNGG 2 cut(s) 326, 670
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 2 cut(s) 332, 409
BseMII CTCAG 3 cut(s) 126, 153, 739
BseNI ACTGG 2 cut(s) 280, 561
BseSI GKGCMC 1 cut(s) 467
BseXI GCAGC 1 cut(s) 532
Bsh1236I CGCG 2 cut(s) 345, 393
BshFI GGCC 1 cut(s) 442
BsiSI CCGG 3 cut(s) 404, 524, 807
BslI CCNNNNNNNGG 2 cut(s) 332, 409
BsmAI GTCTC 4 cut(s) 212, 305, 411, 719
BsmBI CGTCTC 1 cut(s) 719
BsmI GAATGC 2 cut(s) 539, 808
BsnI GGCC 1 cut(s) 442
Bsp1286I GDGCHC 1 cut(s) 467
Bsp143I GATC 7 cut(s) 61, 340, 406, 496, 526, 569, 752
Bsp68I TCGCGA 2 cut(s) 345, 393
BspACI CCGC 3 cut(s) 5, 143, 640
BspANI GGCC 1 cut(s) 442
BspCNI CTCAG 3 cut(s) 127, 154, 738
BspFNI CGCG 2 cut(s) 345, 393
BspLI GGNNCC 1 cut(s) 811
BspPI GGATC 5 cut(s) 56, 414, 504, 534, 577
BspQI GCTCTTC 1 cut(s) 354
BsrI ACTGG 2 cut(s) 280, 561
BssECI CCNNGG 2 cut(s) 326, 670
BssMI GATC 7 cut(s) 61, 340, 406, 496, 526, 569, 752
BssSI CACGAG 1 cut(s) 786
BssT1I CCWWGG 2 cut(s) 326, 670
Bst2BI CACGAG 1 cut(s) 786
Bst4CI ACNGT 4 cut(s) 157, 433, 478, 699
Bst6I CTCTTC 3 cut(s) 231, 354, 722
BstAPI GCANNNNNTGC 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 549
BstDEI CTNAG 4 cut(s) 69, 135, 162, 725
BstF5I GGATG 1 cut(s) 289
BstFNI CGCG 2 cut(s) 345, 393
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 7 cut(s) 64, 343, 409, 499, 529, 572, 755
BstMAI GTCTC 4 cut(s) 212, 305, 411, 719
BstMBI GATC 7 cut(s) 61, 340, 406, 496, 526, 569, 752
BstMWI GCNNNNNNNGC 4 cut(s) 50, 140, 520, 675
BstSCI CCNGG 1 cut(s) 402
BstSLI GKGCMC 1 cut(s) 467
BstUI CGCG 2 cut(s) 345, 393
BstV1I GCAGC 1 cut(s) 532
BstX2I RGATCY 2 cut(s) 406, 496
BstYI RGATCY 2 cut(s) 406, 496
BsuI GTATCC 1 cut(s) 97
BsuRI GGCC 1 cut(s) 442
BtgZI GCGATG 1 cut(s) 261
BtrI CACGTC 1 cut(s) 532
BtsCI GGATG 1 cut(s) 289
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 3 cut(s) 258, 273, 438
BtuMI TCGCGA 2 cut(s) 345, 393
Cac8I GCNNGC 1 cut(s) 549
CaiI CAGNNNCTG 1 cut(s) 161
CfoI GCGC 1 cut(s) 273
Csp6I GTAC 1 cut(s) 177
CviAII CATG 2 cut(s) 493, 535
CviJI RGCY 9 cut(s) 134, 150, 211, 364, 413, 442, 523, 773, 785
CviKI_1 RGCY 9 cut(s) 134, 150, 211, 364, 413, 442, 523, 773, 785
CviQI GTAC 1 cut(s) 177
DdeI CTNAG 4 cut(s) 69, 135, 162, 725
DpnI GATC 7 cut(s) 63, 342, 408, 498, 528, 571, 754
DpnII GATC 7 cut(s) 61, 340, 406, 496, 526, 569, 752
DraI TTTAAA 1 cut(s) 264
DriI GACNNNNNGTC 1 cut(s) 755
Eam1104I CTCTTC 3 cut(s) 231, 354, 722
Eam1105I GACNNNNNGTC 1 cut(s) 755
EarI CTCTTC 3 cut(s) 231, 354, 722
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 326, 670
Eco147I AGGCCT 1 cut(s) 442
EcoT14I CCWWGG 2 cut(s) 326, 670
EcoT22I ATGCAT 1 cut(s) 516
ErhI CCWWGG 2 cut(s) 326, 670
Esp3I CGTCTC 1 cut(s) 719
FaeI CATG 2 cut(s) 496, 538
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FatI CATG 2 cut(s) 492, 534
Fnu4HI GCNGC 2 cut(s) 521, 641
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 2 cut(s) 521, 641
FspBI CTAG 2 cut(s) 222, 308
GlaI GCGC 1 cut(s) 272
GluI GCNGC 2 cut(s) 521, 641
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 1 cut(s) 442
HapII CCGG 3 cut(s) 404, 524, 807
HhaI GCGC 1 cut(s) 273
Hin1II CATG 2 cut(s) 496, 538
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HindIII AAGCTT 1 cut(s) 148
HinfI GANTC 3 cut(s) 116, 313, 709
HpaII CCGG 3 cut(s) 404, 524, 807
HphI GGTGA 2 cut(s) 37, 290
Hpy188I TCNGA 3 cut(s) 18, 163, 728
Hpy188III TCNNGA 9 cut(s) 101, 229, 308, 344, 368, 392, 458, 686, 747
HpyAV CCTTC 6 cut(s) 22, 82, 284, 290, 623, 792
HpyCH4III ACNGT 4 cut(s) 157, 433, 478, 699
HpyCH4IV ACGT 2 cut(s) 179, 531
HpyCH4V TGCA 5 cut(s) 251, 504, 514, 614, 678
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 140, 520, 675
HpyF3I CTNAG 4 cut(s) 69, 135, 162, 725
HpySE526I ACGT 2 cut(s) 179, 531
Hsp92II CATG 2 cut(s) 496, 538
HspAI GCGC 1 cut(s) 271
Kzo9I GATC 7 cut(s) 61, 340, 406, 496, 526, 569, 752
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 1 cut(s) 766
Lsp1109I GCAGC 1 cut(s) 532
LweI GCATC 3 cut(s) 238, 501, 719
MaeI CTAG 2 cut(s) 222, 308
MaeII ACGT 2 cut(s) 179, 531
MalI GATC 7 cut(s) 63, 342, 408, 498, 528, 571, 754
MboI GATC 7 cut(s) 61, 340, 406, 496, 526, 569, 752
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 2 cut(s) 406, 496
MhlI GDGCHC 1 cut(s) 467
MluCI AATT 4 cut(s) 213, 487, 622, 765
MlyI GAGTC 1 cut(s) 307
Mph1103I ATGCAT 1 cut(s) 516
MroXI GAANNNNTTC 1 cut(s) 363
MseI TTAA 4 cut(s) 263, 381, 600, 768
MspI CCGG 3 cut(s) 404, 524, 807
MspR9I CCNGG 1 cut(s) 404
MunI CAATTG 1 cut(s) 213
Mva1269I GAATGC 2 cut(s) 539, 808
MvnI CGCG 2 cut(s) 345, 393
MwoI GCNNNNNNNGC 4 cut(s) 50, 140, 520, 675
NciI CCSGG 1 cut(s) 404
NdeII GATC 7 cut(s) 61, 340, 406, 496, 526, 569, 752
NlaIII CATG 2 cut(s) 496, 538
NlaIV GGNNCC 1 cut(s) 811
NruI TCGCGA 2 cut(s) 345, 393
NsiI ATGCAT 1 cut(s) 516
PceI AGGCCT 1 cut(s) 442
PciSI GCTCTTC 1 cut(s) 354
PctI GAATGC 2 cut(s) 539, 808
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 2 cut(s) 116, 709
PkrI GCNGC 2 cut(s) 522, 642
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
PspN4I GGNNCC 1 cut(s) 811
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 2 cut(s) 406, 496
RruI TCGCGA 2 cut(s) 345, 393
RsaI GTAC 1 cut(s) 178
RsaNI GTAC 1 cut(s) 177
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 4 cut(s) 263, 381, 600, 768
SatI GCNGC 2 cut(s) 521, 641
Sau3AI GATC 7 cut(s) 61, 340, 406, 496, 526, 569, 752
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 1 cut(s) 404
SduI GDGCHC 1 cut(s) 467
SfaNI GCATC 3 cut(s) 238, 501, 719
SmiI ATTTAAAT 1 cut(s) 264
SpeI ACTAGT 1 cut(s) 221
Sse9I AATT 4 cut(s) 213, 487, 622, 765
SseBI AGGCCT 1 cut(s) 442
SsiI CCGC 3 cut(s) 5, 143, 640
SspMI CTAG 2 cut(s) 222, 308
StuI AGGCCT 1 cut(s) 442
StyD4I CCNGG 1 cut(s) 402
StyI CCWWGG 2 cut(s) 326, 670
SwaI ATTTAAAT 1 cut(s) 264
TaaI ACNGT 4 cut(s) 157, 433, 478, 699
TaiI ACGT 2 cut(s) 182, 534
TasI AATT 4 cut(s) 213, 487, 622, 765
TauI GCSGC 1 cut(s) 643
TfiI GAWTC 2 cut(s) 116, 709
Tru1I TTAA 4 cut(s) 263, 381, 600, 768
Tru9I TTAA 4 cut(s) 263, 381, 600, 768
TscAI CASTG 3 cut(s) 258, 280, 438
TseI GCWGC 1 cut(s) 520
TspDTI ATGAA 4 cut(s) 249, 372, 481, 669
TspRI CASTG 3 cut(s) 258, 280, 438
XapI RAATTY 2 cut(s) 487, 622
XbaI TCTAGA 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 324
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 2 cut(s) 222, 308
Zsp2I ATGCAT 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.