AT3G21945

cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002319
Physical Location & Seq
Forward (+)
7732263 .. 7733294
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G21945.1

Sequence Viewer

Length: 462 bp
ATGTCTTCATCCTTTGTATATAAAAGCCTCTTTTTGGTCCCTATCTTGGCCGTGGTGGCCATGCAACTCTCATTTGTACAGAGTGTTTTGTCCCTAAACCAGACAAATGAGTATCTACACCATTTATGCCTTAATCGTCAAGGGACATACGAGTCAGGTAGTAAATACGCGAGAGACCTTAACAATCTCATCCAAATGATCTCTATCTCAATTTTAGGTGACGTTGTCGTCTTTAGCGGTGACAATTCCATCTACGTCAAGCTCCAGTGCCGGGGGGACTCCTCCACATCCACGTGCCGCTCTTGCCTTGACACCGCCTTCTCTGGGGTTAGTAGGAGATGTCTGAACAACAAAGGAAGAATAATATGGTACGACAACTGTGTTCTCACAATTTTAACATTTCATACGTATGGCGAGATCGATTACCAGAACAATTTCTATATTTCAACGCAAAGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

153

Amino Acids

17.34

Weight (kDa)

8.22

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 43 - 130 1.1e-16 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 151, 227
AccBSI CCGCTC 1 cut(s) 300
AccII CGCG 1 cut(s) 170
AciI CCGC 3 cut(s) 237, 298, 315
AcoI YGGCCR 2 cut(s) 48, 57
AcvI CACGTG 1 cut(s) 294
AfaI GTAC 2 cut(s) 78, 371
AfiI CCNNNNNNNGG 4 cut(s) 34, 46, 271, 324
AgsI TTSAA 1 cut(s) 447
AluBI AGCT 1 cut(s) 262
AluI AGCT 1 cut(s) 262
Alw26I GTCTC 1 cut(s) 168
AoxI GGCC 2 cut(s) 48, 57
Asp700I GAANNNNTTC 1 cut(s) 434
AspS9I GGNCC 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 2 cut(s) 230, 251
AvaII GGWCC 1 cut(s) 37
BalI TGGCCA 1 cut(s) 59
BbrPI CACGTG 1 cut(s) 294
BccI CCATC 1 cut(s) 257
BceAI ACGGC 1 cut(s) 35
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 168
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 1 cut(s) 298
BlsI GCNGC 1 cut(s) 299
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 272
Bsa29I ATCGAT 1 cut(s) 420
BsaAI YACGTR 2 cut(s) 294, 408
BsaBI GATNNNNATC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 51, 271
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 46, 271, 324
Bse1I ACTGG 1 cut(s) 265
Bse8I GATNNNNATC 1 cut(s) 203
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 2 cut(s) 51, 271
BseGI GGATG 4 cut(s) 8, 189, 287, 462
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 4 cut(s) 34, 46, 271, 324
BseNI ACTGG 1 cut(s) 265
BseRI GAGGAG 1 cut(s) 271
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 2 cut(s) 50, 59
BshVI ATCGAT 1 cut(s) 420
BsiSI CCGG 1 cut(s) 271
BslFI GGGAC 4 cut(s) 23, 76, 157, 290
BslI CCNNNNNNNGG 4 cut(s) 34, 46, 271, 324
BsmAI GTCTC 1 cut(s) 168
BsmFI GGGAC 4 cut(s) 23, 76, 157, 290
BsnI GGCC 2 cut(s) 50, 59
Bso31I GGTCTC 1 cut(s) 168
Bsp1407I TGTACA 1 cut(s) 76
Bsp143I GATC 2 cut(s) 198, 417
BspACI CCGC 3 cut(s) 237, 298, 315
BspANI GGCC 2 cut(s) 50, 59
BspDI ATCGAT 1 cut(s) 420
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 1 cut(s) 39
BspTNI GGTCTC 1 cut(s) 168
BsrBI CCGCTC 1 cut(s) 300
BsrGI TGTACA 1 cut(s) 76
BsrI ACTGG 1 cut(s) 265
BssECI CCNNGG 2 cut(s) 51, 271
BssMI GATC 2 cut(s) 198, 417
Bst4CI ACNGT 1 cut(s) 380
BstAUI TGTACA 1 cut(s) 76
BstBAI YACGTR 2 cut(s) 294, 408
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 4 cut(s) 8, 189, 287, 462
BstFNI CGCG 1 cut(s) 170
BstKTI GATC 2 cut(s) 201, 420
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 2 cut(s) 198, 417
BstMWI GCNNNNNNNGC 2 cut(s) 56, 303
BstSCI CCNGG 1 cut(s) 270
BstSNI TACGTA 1 cut(s) 408
BstUI CGCG 1 cut(s) 170
Bsu15I ATCGAT 1 cut(s) 420
BsuRI GGCC 2 cut(s) 50, 59
BsuTUI ATCGAT 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 4 cut(s) 8, 189, 287, 462
BtsIMutI CAGTG 1 cut(s) 272
Cfr13I GGNCC 1 cut(s) 37
ClaI ATCGAT 1 cut(s) 420
Csp6I GTAC 2 cut(s) 77, 370
CviAII CATG 1 cut(s) 61
CviJI RGCY 4 cut(s) 27, 50, 59, 262
CviKI_1 RGCY 4 cut(s) 27, 50, 59, 262
CviQI GTAC 2 cut(s) 77, 370
DpnI GATC 2 cut(s) 200, 419
DpnII GATC 2 cut(s) 198, 417
DrdI GACNNNNNNGTC 2 cut(s) 151, 227
DseDI GACNNNNNNGTC 2 cut(s) 151, 227
EaeI YGGCCR 2 cut(s) 48, 57
Eco105I TACGTA 1 cut(s) 408
Eco31I GGTCTC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 37
Eco72I CACGTG 1 cut(s) 294
FaeI CATG 1 cut(s) 64
FaiI YATR 9 cut(s) 19, 21, 62, 127, 148, 367, 405, 411, 441
FaqI GGGAC 4 cut(s) 23, 76, 157, 290
FatI CATG 1 cut(s) 60
Fnu4HI GCNGC 1 cut(s) 298
FokI GGATG 2 cut(s) 176, 274
Fsp4HI GCNGC 1 cut(s) 298
GluI GCNGC 1 cut(s) 298
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 2 cut(s) 50, 59
HapII CCGG 1 cut(s) 271
Hin1II CATG 1 cut(s) 64
HinfI GANTC 2 cut(s) 152, 278
HpaII CCGG 1 cut(s) 271
HphI GGTGA 2 cut(s) 230, 251
Hpy188I TCNGA 1 cut(s) 345
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 1 cut(s) 380
HpyCH4IV ACGT 4 cut(s) 222, 255, 293, 407
HpyCH4V TGCA 1 cut(s) 64
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 303
HpySE526I ACGT 4 cut(s) 222, 255, 293, 407
Hsp92II CATG 1 cut(s) 64
Kzo9I GATC 2 cut(s) 198, 417
LmnI GCTCC 1 cut(s) 267
LpnPI CCDG 6 cut(s) 113, 141, 278, 284, 309, 440
MaeII ACGT 4 cut(s) 222, 255, 293, 407
MaeIII GTNAC 2 cut(s) 218, 239
MalI GATC 2 cut(s) 200, 419
MbiI CCGCTC 1 cut(s) 300
MboI GATC 2 cut(s) 198, 417
MboII GAAGA 1 cut(s) 369
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 4 cut(s) 210, 244, 390, 433
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 2 cut(s) 161, 272
MnlI CCTC 2 cut(s) 38, 292
Mox20I TGGCCA 1 cut(s) 59
MroXI GAANNNNTTC 1 cut(s) 434
MscI TGGCCA 1 cut(s) 59
MseI TTAA 3 cut(s) 132, 180, 395
MslI CAYNNNNRTG 3 cut(s) 194, 292, 408
Msp20I TGGCCA 1 cut(s) 59
MspI CCGG 1 cut(s) 271
MspR9I CCNGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 2 cut(s) 56, 303
NciI CCSGG 1 cut(s) 272
NdeII GATC 2 cut(s) 198, 417
NlaIII CATG 1 cut(s) 64
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 218, 239
PdmI GAANNNNTTC 1 cut(s) 434
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 1 cut(s) 299
PleI GAGTC 2 cut(s) 160, 272
PmaCI CACGTG 1 cut(s) 294
PmlI CACGTG 1 cut(s) 294
PpsI GAGTC 2 cut(s) 160, 272
Ppu21I YACGTR 2 cut(s) 294, 408
PspCI CACGTG 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 1 cut(s) 37
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 2 cut(s) 78, 371
RsaNI GTAC 2 cut(s) 77, 370
RseI CAYNNNNRTG 3 cut(s) 194, 292, 408
SaqAI TTAA 3 cut(s) 132, 180, 395
SatI GCNGC 1 cut(s) 298
Sau3AI GATC 2 cut(s) 198, 417
Sau96I GGNCC 1 cut(s) 37
SchI GAGTC 2 cut(s) 161, 272
ScrFI CCNGG 1 cut(s) 272
SetI ASST 8 cut(s) 160, 180, 220, 225, 258, 264, 296, 410
SfiI GGCCNNNNNGGCC 1 cut(s) 56
SinI GGWCC 1 cut(s) 37
SmiMI CAYNNNNRTG 3 cut(s) 194, 292, 408
SnaBI TACGTA 1 cut(s) 408
Sse9I AATT 4 cut(s) 210, 244, 390, 433
SsiI CCGC 3 cut(s) 237, 298, 315
StyD4I CCNGG 1 cut(s) 270
TaaI ACNGT 1 cut(s) 380
TaiI ACGT 4 cut(s) 225, 258, 296, 410
TaqI TCGA 1 cut(s) 420
TasI AATT 4 cut(s) 210, 244, 390, 433
TatI WGTACW 1 cut(s) 76
TauI GCSGC 1 cut(s) 300
Tru1I TTAA 3 cut(s) 132, 180, 395
Tru9I TTAA 3 cut(s) 132, 180, 395
TscAI CASTG 1 cut(s) 272
TseFI GTSAC 2 cut(s) 218, 239
Tsp45I GTSAC 2 cut(s) 218, 239
TspDTI ATGAA 1 cut(s) 392
TspRI CASTG 1 cut(s) 272
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.