AT3G22057

cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002319
Physical Location & Seq
Forward (+)
7769346 .. 7770261
916 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22057.1

Sequence Viewer

Length: 525 bp
ATGAACAAAACAATGTACTCTTCATATTCTTTATCCAAACGTCTTGTTTCTATCCCTATCTTGGCTATACAACTCCTTCTCATACGCAGTGTCTCGTCACTGAACCTTACCAATGACTATCTTAACCACAAATGCCTCGTCAGTCAAGGGAAATATAGGCCTGGAGATAAGTACGAGGACAACCTCAACTTTCTCACCCGTGAATGCCGCGGCGACTCCTACGACTCCAAGTGCCTCTCCTGCTATGCCACCGCCCTTTCCGGGATCATCCCCAAGGAAGAACGATTAAAGGAATGTTTTCTCTATGCACAACCCAAACAACATGATCGAGGACACGGAATTGTTCAACAAGAAAACAAGGGATTTCCTCTACGAGCTCATGCTGGAAGCCACTACACCCAACAGGACAATGATGCTTTACGCAGCAGGGGAGAAGAAGCTAGGGACAAAGAAATTGTATGCAATGGTACAGTGTGCGCAAGACATATTGCGATGAAAGGGTTGTTTGGAATGGAGTATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

174

Amino Acids

19.78

Weight (kDa)

8.8

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 478
AccII CGCG 1 cut(s) 210
AciI CCGC 3 cut(s) 208, 210, 252
AclWI GGATC 1 cut(s) 272
AfaI GTAC 3 cut(s) 17, 173, 469
AfiI CCNNNNNNNGG 2 cut(s) 61, 261
AgsI TTSAA 1 cut(s) 347
AjnI CCWGG 1 cut(s) 160
AluBI AGCT 2 cut(s) 377, 440
AluI AGCT 2 cut(s) 377, 440
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 1 cut(s) 97
AlwI GGATC 1 cut(s) 272
AoxI GGCC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 423
ArsI GACNNNNNNTTYG 2 cut(s) 123, 155
Asp700I GAANNNNTTC 1 cut(s) 297
AspLEI GCGC 1 cut(s) 479
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 1 cut(s) 187
BanII GRGCYC 1 cut(s) 379
Bbv12I GWGCWC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 435
BciT130I CCWGG 1 cut(s) 162
BcnI CCSGG 1 cut(s) 262
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 1 cut(s) 441
BisI GCNGC 3 cut(s) 208, 211, 424
BlsI GCNGC 3 cut(s) 209, 212, 425
Bme1390I CCNGG 2 cut(s) 162, 262
BmrFI CCNGG 2 cut(s) 162, 262
BmsI GCATC 1 cut(s) 403
BpmI CTGGAG 1 cut(s) 183
BpuMI CCSGG 1 cut(s) 262
BsaJI CCNNGG 2 cut(s) 208, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 261
Bse3DI GCAATG 1 cut(s) 469
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 2 cut(s) 208, 273
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 2 cut(s) 61, 261
BseMI GCAATG 1 cut(s) 469
BseXI GCAGC 1 cut(s) 435
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 379
BsiSI CCGG 1 cut(s) 261
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 2 cut(s) 61, 261
BsmAI GTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 458
BsmI GAATGC 1 cut(s) 209
BsnI GGCC 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 379
Bsp143I GATC 2 cut(s) 264, 325
BspACI CCGC 3 cut(s) 208, 210, 252
BspANI GGCC 1 cut(s) 160
BspFNI CGCG 1 cut(s) 210
BspPI GGATC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 469
BssECI CCNNGG 2 cut(s) 208, 273
BssMI GATC 2 cut(s) 264, 325
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 1 cut(s) 472
Bst6I CTCTTC 1 cut(s) 25
BstDSI CCRYGG 1 cut(s) 208
BstF5I GGATG 1 cut(s) 267
BstFNI CGCG 1 cut(s) 210
BstHHI GCGC 1 cut(s) 479
BstKTI GATC 2 cut(s) 267, 328
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 2 cut(s) 264, 325
BstMWI GCNNNNNNNGC 1 cut(s) 240
BstNI CCWGG 1 cut(s) 162
BstSCI CCNGG 2 cut(s) 160, 260
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 1 cut(s) 435
BsuRI GGCC 1 cut(s) 160
BtgI CCRYGG 1 cut(s) 208
BtgZI GCGATG 1 cut(s) 506
BtsCI GGATG 1 cut(s) 267
BtsI GCAGTG 1 cut(s) 94
BtsIMutI CAGTG 3 cut(s) 94, 98, 477
CfoI GCGC 1 cut(s) 479
Cfr42I CCGCGG 1 cut(s) 211
Csp6I GTAC 3 cut(s) 16, 172, 468
CviAII CATG 2 cut(s) 323, 380
CviJI RGCY 5 cut(s) 65, 160, 377, 390, 440
CviKI_1 RGCY 5 cut(s) 65, 160, 377, 390, 440
CviQI GTAC 3 cut(s) 16, 172, 468
DpnI GATC 2 cut(s) 266, 327
DpnII GATC 2 cut(s) 264, 325
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 273
Eco147I AGGCCT 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 2 cut(s) 326, 383
FaqI GGGAC 1 cut(s) 458
FatI CATG 2 cut(s) 322, 379
Fnu4HI GCNGC 3 cut(s) 208, 211, 424
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 3 cut(s) 208, 211, 424
FspBI CTAG 1 cut(s) 441
FspI TGCGCA 1 cut(s) 478
GlaI GCGC 1 cut(s) 478
GluI GCNGC 3 cut(s) 208, 211, 424
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 1 cut(s) 160
HapII CCGG 1 cut(s) 261
HhaI GCGC 1 cut(s) 479
Hin1II CATG 2 cut(s) 326, 383
Hin6I GCGC 1 cut(s) 477
HinP1I GCGC 1 cut(s) 477
HinfI GANTC 2 cut(s) 215, 224
HpaII CCGG 1 cut(s) 261
HphI GGTGA 1 cut(s) 187
HpyAV CCTTC 1 cut(s) 86
HpyCH4III ACNGT 1 cut(s) 472
HpyCH4IV ACGT 1 cut(s) 40
HpyCH4V TGCA 2 cut(s) 308, 462
HpyF10VI GCNNNNNNNGC 1 cut(s) 240
HpySE526I ACGT 1 cut(s) 40
Hsp92II CATG 2 cut(s) 326, 383
HspAI GCGC 1 cut(s) 477
KspI CCGCGG 1 cut(s) 211
Kzo9I GATC 2 cut(s) 264, 325
LpnPI CCDG 7 cut(s) 147, 174, 253, 274, 369, 389, 412
Lsp1109I GCAGC 1 cut(s) 435
LweI GCATC 1 cut(s) 403
MaeI CTAG 1 cut(s) 441
MaeII ACGT 1 cut(s) 40
MaeIII GTNAC 1 cut(s) 96
MalI GATC 2 cut(s) 266, 327
MboI GATC 2 cut(s) 264, 325
MboII GAAGA 3 cut(s) 12, 290, 446
MhlI GDGCHC 1 cut(s) 379
MluCI AATT 2 cut(s) 339, 453
MlyI GAGTC 2 cut(s) 209, 218
MnlI CCTC 6 cut(s) 146, 169, 194, 245, 323, 378
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 2 cut(s) 123, 287
MspA1I CMGCKG 1 cut(s) 210
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 2 cut(s) 162, 262
Mva1269I GAATGC 1 cut(s) 209
MvaI CCWGG 1 cut(s) 162
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 240
NciI CCSGG 1 cut(s) 262
NdeII GATC 2 cut(s) 264, 325
NlaIII CATG 2 cut(s) 326, 383
NmuCI GTSAC 1 cut(s) 96
NsbI TGCGCA 1 cut(s) 478
PceI AGGCCT 1 cut(s) 160
PctI GAATGC 1 cut(s) 209
PdmI GAANNNNTTC 1 cut(s) 297
PfoI TCCNGGA 1 cut(s) 260
PkrI GCNGC 3 cut(s) 209, 212, 425
PleI GAGTC 2 cut(s) 209, 218
PpsI GAGTC 2 cut(s) 209, 218
Psp124BI GAGCTC 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
RsaI GTAC 3 cut(s) 17, 173, 469
RsaNI GTAC 3 cut(s) 16, 172, 468
SacI GAGCTC 1 cut(s) 379
SacII CCGCGG 1 cut(s) 211
SaqAI TTAA 2 cut(s) 123, 287
SatI GCNGC 3 cut(s) 208, 211, 424
Sau3AI GATC 2 cut(s) 264, 325
SchI GAGTC 2 cut(s) 209, 218
ScrFI CCNGG 2 cut(s) 162, 262
SduI GDGCHC 1 cut(s) 379
SetI ASST 5 cut(s) 43, 108, 186, 379, 442
SfaNI GCATC 1 cut(s) 403
Sfr303I CCGCGG 1 cut(s) 211
SgrBI CCGCGG 1 cut(s) 211
Sse9I AATT 2 cut(s) 339, 453
SseBI AGGCCT 1 cut(s) 160
SsiI CCGC 3 cut(s) 208, 210, 252
SspMI CTAG 1 cut(s) 441
SstI GAGCTC 1 cut(s) 379
StuI AGGCCT 1 cut(s) 160
StyD4I CCNGG 2 cut(s) 160, 260
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 1 cut(s) 472
TaiI ACGT 1 cut(s) 43
TaqI TCGA 1 cut(s) 328
TasI AATT 2 cut(s) 339, 453
TatI WGTACW 1 cut(s) 15
TauI GCSGC 2 cut(s) 210, 213
Tru1I TTAA 2 cut(s) 123, 287
Tru9I TTAA 2 cut(s) 123, 287
TscAI CASTG 3 cut(s) 94, 105, 477
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 1 cut(s) 423
Tsp45I GTSAC 1 cut(s) 96
TspDTI ATGAA 3 cut(s) 12, 17, 509
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 3 cut(s) 94, 105, 477
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.