AT3G22020

cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002319
Physical Location & Seq
Forward (+)
7756458 .. 7757491
1034 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22020.1

Sequence Viewer

Length: 624 bp
ATGCGTATTTGCACCACAAATGCCTCACTCGTCAAGGGAAATATAAGCCGAGAAGCCCGTACGAGGAAAACCTTAATTATCTCATCGATCATATCTCTAAGTATCATTTTCGGGACGGTTTCAGACACAGCGCATGGTGAGGCTCCGAATTCCGGCAACTTTGTATTCCAATGCCGTGGAGACTCCTACGGGTCTATATGCGGTTCTTGCTATGCCACCGCTGTGGCCGGGCTTCGTAAGAGATGTCAGAGATACAAAGGAGCAATAATATGGTATGATCAATGTTTTCTCAAAGTAAGTACGATCAACTCCCCTCCAACGAAGATGGATTACGAGAATACTTTTTCTATGCACAACCCAAACAACATCAACGGGGATACTCAGTTGTTCAACCAAAAGATGAAGAATTTCCTCTATGAGCTAGCGCTGAAAGCGGATAAACCCAAAGCGGACGGTACCGGTTTGCTATATTACGCGGCAGGGACGAAGAGGCTCGGGAAGAATAAACTGTATGCAATGGTGCAGTGTACTTTAGACATATTTCATTGTAAGGTTTGTTTGGAATGGAGTATTAAGGAGCTTTCAAAGTGTTGTGAAGAACACATTCTCTTGACAACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

207

Amino Acids

23.11

Weight (kDa)

9.1

Isoelectric Point (pI)

29.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 42 - 101 3.8e-12 Salt stress response/antifungal
Stress-antifung PF01657 125 - 199 8.7e-07 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 455
AccB1I GGYRCC 1 cut(s) 455
AccII CGCG 1 cut(s) 476
AciI CCGC 5 cut(s) 201, 219, 434, 449, 476
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 2 cut(s) 148, 406
AfaI GTAC 4 cut(s) 61, 301, 457, 529
AfeI AGCGCT 1 cut(s) 426
AfiI CCNNNNNNNGG 2 cut(s) 63, 152
AgeI ACCGGT 1 cut(s) 458
AgsI TTSAA 2 cut(s) 391, 585
AleI CACNNNNGTG 1 cut(s) 221
AluBI AGCT 2 cut(s) 421, 580
AluI AGCT 2 cut(s) 421, 580
Alw26I GTCTC 1 cut(s) 174
Ama87I CYCGRG 1 cut(s) 494
Aor51HI AGCGCT 1 cut(s) 426
AoxI GGCC 1 cut(s) 225
ApoI RAATTY 2 cut(s) 148, 406
AsiGI ACCGGT 1 cut(s) 458
Asp700I GAANNNNTTC 2 cut(s) 407, 603
Asp718I GGTACC 1 cut(s) 455
AspLEI GCGC 2 cut(s) 133, 427
AsuC2I CCSGG 1 cut(s) 229
AsuHPI GGTGA 1 cut(s) 149
AsuNHI GCTAGC 1 cut(s) 421
AvaI CYCGRG 1 cut(s) 494
BanI GGYRCC 1 cut(s) 455
BarI GAAGNNNNNNTAC 2 cut(s) 314, 346
BccI CCATC 1 cut(s) 319
BceAI ACGGC 1 cut(s) 159
BciVI GTATCC 1 cut(s) 370
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 1 cut(s) 422
BfoI RGCGCY 1 cut(s) 428
BfuI GTATCC 1 cut(s) 370
BisI GCNGC 1 cut(s) 477
BlsI GCNGC 1 cut(s) 478
Bme1390I CCNGG 1 cut(s) 229
BmeT110I CYCGRG 1 cut(s) 494
BmiI GGNNCC 2 cut(s) 144, 457
BmrFI CCNGG 1 cut(s) 229
BmtI GCTAGC 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 229
Bsa29I ATCGAT 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 175
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 152
Bse118I RCCGGY 1 cut(s) 458
Bse3DI GCAATG 1 cut(s) 522
BseCI ATCGAT 1 cut(s) 86
BseDI CCNNGG 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 63, 152
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 1 cut(s) 395
BsgI GTGCAG 1 cut(s) 542
Bsh1236I CGCG 1 cut(s) 476
BshFI GGCC 1 cut(s) 227
BshNI GGYRCC 1 cut(s) 455
BshTI ACCGGT 1 cut(s) 458
BshVI ATCGAT 1 cut(s) 86
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 3 cut(s) 153, 228, 459
BsiWI CGTACG 1 cut(s) 59
BslFI GGGAC 2 cut(s) 127, 496
BslI CCNNNNNNNGG 2 cut(s) 63, 152
BsmAI GTCTC 1 cut(s) 174
BsmFI GGGAC 2 cut(s) 127, 496
BsnI GGCC 1 cut(s) 227
BsoBI CYCGRG 1 cut(s) 494
Bsp143I GATC 3 cut(s) 87, 277, 303
BspACI CCGC 5 cut(s) 201, 219, 434, 449, 476
BspANI GGCC 1 cut(s) 227
BspCNI CTCAG 1 cut(s) 394
BspDI ATCGAT 1 cut(s) 86
BspFNI CGCG 1 cut(s) 476
BspLI GGNNCC 2 cut(s) 144, 457
BspOI GCTAGC 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 522
BsrFI RCCGGY 1 cut(s) 458
BssAI RCCGGY 1 cut(s) 458
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 3 cut(s) 87, 277, 303
Bst4CI ACNGT 3 cut(s) 118, 455, 510
Bst6I CTCTTC 1 cut(s) 482
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 2 cut(s) 98, 381
BstDSI CCRYGG 1 cut(s) 175
BstFNI CGCG 1 cut(s) 476
BstH2I RGCGCY 1 cut(s) 428
BstHHI GCGC 2 cut(s) 133, 427
BstKTI GATC 3 cut(s) 90, 280, 306
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 3 cut(s) 87, 277, 303
BstMWI GCNNNNNNNGC 2 cut(s) 207, 431
BstSCI CCNGG 1 cut(s) 227
BstUI CGCG 1 cut(s) 476
BstXI CCANNNNNNTGG 2 cut(s) 176, 223
Bsu15I ATCGAT 1 cut(s) 86
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 1 cut(s) 227
BsuTUI ATCGAT 1 cut(s) 86
BtgI CCRYGG 1 cut(s) 175
BtsI GCAGTG 1 cut(s) 530
BtsIMutI CAGTG 1 cut(s) 530
Cac8I GCNNGC 1 cut(s) 423
CfoI GCGC 2 cut(s) 133, 427
Cfr10I RCCGGY 1 cut(s) 458
ClaI ATCGAT 1 cut(s) 86
Csp6I GTAC 4 cut(s) 60, 300, 456, 528
CspAI ACCGGT 1 cut(s) 458
CviAII CATG 1 cut(s) 134
CviJI RGCY 8 cut(s) 48, 56, 143, 227, 232, 421, 493, 580
CviKI_1 RGCY 8 cut(s) 48, 56, 143, 227, 232, 421, 493, 580
CviQI GTAC 4 cut(s) 60, 300, 456, 528
DdeI CTNAG 2 cut(s) 98, 381
DpnI GATC 3 cut(s) 89, 279, 305
DpnII GATC 3 cut(s) 87, 277, 303
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 482
EarI CTCTTC 1 cut(s) 482
Eco47III AGCGCT 1 cut(s) 426
Eco88I CYCGRG 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 148
FaeI CATG 1 cut(s) 137
FaqI GGGAC 2 cut(s) 127, 496
FatI CATG 1 cut(s) 133
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 1 cut(s) 477
Fsp4HI GCNGC 1 cut(s) 477
FspBI CTAG 1 cut(s) 422
GlaI GCGC 2 cut(s) 132, 426
GluI GCNGC 1 cut(s) 477
HaeII RGCGCY 1 cut(s) 428
HaeIII GGCC 1 cut(s) 227
HapII CCGG 3 cut(s) 153, 228, 459
HhaI GCGC 2 cut(s) 133, 427
Hin1II CATG 1 cut(s) 137
Hin6I GCGC 2 cut(s) 131, 425
HinP1I GCGC 2 cut(s) 131, 425
HinfI GANTC 1 cut(s) 182
HpaII CCGG 3 cut(s) 153, 228, 459
HphI GGTGA 1 cut(s) 149
Hpy166II GTNNAC 1 cut(s) 528
Hpy188I TCNGA 3 cut(s) 124, 147, 249
Hpy188III TCNNGA 3 cut(s) 112, 496, 610
Hpy8I GTNNAC 1 cut(s) 528
HpyCH4III ACNGT 3 cut(s) 118, 455, 510
HpyCH4V TGCA 4 cut(s) 12, 352, 515, 523
HpyF10VI GCNNNNNNNGC 2 cut(s) 207, 431
HpyF3I CTNAG 2 cut(s) 98, 381
Hsp92II CATG 1 cut(s) 137
HspAI GCGC 2 cut(s) 131, 425
KpnI GGTACC 1 cut(s) 459
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 3 cut(s) 87, 277, 303
LmnI GCTCC 3 cut(s) 148, 260, 577
LpnPI CCDG 4 cut(s) 166, 241, 465, 472
MaeI CTAG 1 cut(s) 422
MalI GATC 3 cut(s) 89, 279, 305
MboI GATC 3 cut(s) 87, 277, 303
MboII GAAGA 5 cut(s) 334, 415, 499, 511, 608
MluCI AATT 3 cut(s) 75, 148, 406
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 341
MnlI CCTC 6 cut(s) 34, 57, 133, 324, 422, 483
MroXI GAANNNNTTC 2 cut(s) 407, 603
MseI TTAA 3 cut(s) 74, 573, 622
MslI CAYNNNNRTG 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 3 cut(s) 153, 228, 459
MspR9I CCNGG 1 cut(s) 229
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 2 cut(s) 207, 431
NciI CCSGG 1 cut(s) 229
NdeII GATC 3 cut(s) 87, 277, 303
NheI GCTAGC 1 cut(s) 421
NlaIII CATG 1 cut(s) 137
NlaIV GGNNCC 2 cut(s) 144, 457
NmeAIII GCCGAG 1 cut(s) 74
OliI CACNNNNGTG 1 cut(s) 221
PdmI GAANNNNTTC 2 cut(s) 407, 603
Pfl23II CGTACG 1 cut(s) 59
PinAI ACCGGT 1 cut(s) 458
PkrI GCNGC 1 cut(s) 478
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
PspLI CGTACG 1 cut(s) 59
PspN4I GGNNCC 2 cut(s) 144, 457
RsaI GTAC 4 cut(s) 61, 301, 457, 529
RsaNI GTAC 4 cut(s) 60, 300, 456, 528
RseI CAYNNNNRTG 1 cut(s) 221
SaqAI TTAA 3 cut(s) 74, 573, 622
SatI GCNGC 1 cut(s) 477
Sau3AI GATC 3 cut(s) 87, 277, 303
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 1 cut(s) 229
SetI ASST 4 cut(s) 74, 423, 555, 582
SmiMI CAYNNNNRTG 1 cut(s) 221
Sse9I AATT 3 cut(s) 75, 148, 406
SsiI CCGC 5 cut(s) 201, 219, 434, 449, 476
SspMI CTAG 1 cut(s) 422
StyD4I CCNGG 1 cut(s) 227
TaaI ACNGT 3 cut(s) 118, 455, 510
TaqI TCGA 1 cut(s) 86
TasI AATT 3 cut(s) 75, 148, 406
TatI WGTACW 1 cut(s) 527
TauI GCSGC 1 cut(s) 479
Tru1I TTAA 3 cut(s) 74, 573, 622
Tru9I TTAA 3 cut(s) 74, 573, 622
TscAI CASTG 1 cut(s) 530
TspDTI ATGAA 2 cut(s) 416, 533
TspRI CASTG 1 cut(s) 530
XapI RAATTY 2 cut(s) 148, 406
XmnI GAANNNNTTC 2 cut(s) 407, 603
XspI CTAG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.