AT3G28050

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004267
Physical Location & Seq
Forward (+)
10442608 .. 10445431
2824 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G28050.1

Sequence Viewer

Length: 1104 bp
ATGGCGCGGAAATACTTTCAGAGAGAAGTTTTGCCGGTAACGGCTTTGGTAATAATGGAATGTGCAAACGTTGGTTTAAACACTCTGTTTAAAGCAGCAACTTTGAAAGGCATGAGCTTTCATGTCTTCATCGTTTACTCTTATGGTCTCGCTGCTCTGCTTCTTCTTCCTTCTCTGTTTTGCTCTTTCAGATCAAGAACTCTTCCACCGATGAATTTCTCTATTCTCTACAAAATCGTGCTCTTAGGGATTATTGGATGCTGTTCAAACATAATGGGATACACAGGGATAAATTATAGTTCTCCAACACTTGCTTCTGCAATCAGCAACCTCACTCCTGCTTTCACCTTCTTGCTTGCAGTCGTTTTCAGGATGGAGAGTGTGTCTTTTAAGAGAACAAGTAGTGTGGCTAAAATGTTAGGAACCGTTGTTTCTATTGGAGGTGCATTTATAGTGACTCTTTATAATGGTCCTGTTGTGATTGCTAAATCGCCACCGTCTGTTTCTTTGCGATCGCAATCGACTAACCCTAATTGGATACTTGGTGCGGGTTTCCTTGCGGTCGAGTATTTCTGTGTTCCATTGTGGTACATTGTTCAGACTCAAATCATGAGAGAGTATCCGGCCGAGTTTACCGTTGTTTGCTTTTATAGCATAGGCGTTAGCTTTTGGACCGCGCTAGTCACATTGTTCACTGAAGGGAATGACTTGGGTGCATGGAAGATAAAACCGAATATAGCTTTAGTGTCGATCGTTTGCTCGGGATTGTTTGGATCTTGCATAAACAATACTATCCACACGTGGGCATTGCGGATCAAGGGGCCTTTATTTGTTGCAATGTTCAAGCCATTGTCTATTGCAATTGCTGTTGCAATGGGAGTTATCTTCCTGCGAGATTCTCTCTACATCGGCAGCTTGATCGGGGCAACGGTAATAACAATCGGGTTTTACACGGTAATGTGGGGAAAAGCCAAGGAAGTGGCCTTGGTCGAAGATGATAACAAGGCTAACCACGAGGAGGCCAATGAAGCTGACCTTGATTCTCCATCGGGTTCACAGAAGGCTCCTCTCTTGGAAAGTTACAAGAACGATGAACATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

367

Amino Acids

40.06

Weight (kDa)

8.69

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 20 - 153 1.9e-08 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
AccII CGCG 2 cut(s) 7, 677
AciI CCGC 5 cut(s) 7, 548, 560, 675, 811
AclI AACGTT 1 cut(s) 69
AclWI GGATC 2 cut(s) 781, 821
AcoI YGGCCR 1 cut(s) 624
AcsI RAATTY 1 cut(s) 214
AcuI CTGAAG 1 cut(s) 717
AcvI CACGTG 1 cut(s) 801
AfaI GTAC 1 cut(s) 590
AfiI CCNNNNNNNGG 2 cut(s) 802, 1018
AflIII ACRYGT 2 cut(s) 798, 1096
AgsI TTSAA 3 cut(s) 106, 267, 844
AjuI GAANNNNNNNTTGG 2 cut(s) 298, 330
AluBI AGCT 5 cut(s) 117, 666, 740, 915, 1031
AluI AGCT 5 cut(s) 117, 666, 740, 915, 1031
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 2 cut(s) 781, 821
Ama87I CYCGRG 1 cut(s) 760
AoxI GGCC 4 cut(s) 624, 821, 981, 1020
ApeKI GCWGC 3 cut(s) 95, 152, 912
ApoI RAATTY 1 cut(s) 214
AsiSI GCGATCGC 1 cut(s) 515
AspLEI GCGC 2 cut(s) 7, 679
AspS9I GGNCC 3 cut(s) 470, 672, 821
AsuHPI GGTGA 1 cut(s) 337
AvaI CYCGRG 1 cut(s) 760
AvaII GGWCC 2 cut(s) 470, 672
BauI CACGAG 1 cut(s) 1013
BbrPI CACGTG 1 cut(s) 801
BbsI GAAGAC 1 cut(s) 118
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 3 cut(s) 107, 139, 924
BccI CCATC 2 cut(s) 367, 1054
BceAI ACGGC 1 cut(s) 57
BcgI CGANNNNNNTGC 2 cut(s) 901, 935
BciVI GTATCC 3 cut(s) 272, 531, 630
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 680
BfuI GTATCC 3 cut(s) 272, 531, 630
BisI GCNGC 3 cut(s) 96, 153, 913
BlsI GCNGC 3 cut(s) 97, 154, 914
Bme18I GGWCC 2 cut(s) 470, 672
BmeT110I CYCGRG 1 cut(s) 760
BmgT120I GGNCC 3 cut(s) 470, 672, 821
BmiI GGNNCC 3 cut(s) 424, 822, 1065
BmsI GCATC 1 cut(s) 248
BpiI GAAGAC 1 cut(s) 118
BplI GAGNNNNNCTC 2 cut(s) 885, 917
BsaAI YACGTR 1 cut(s) 801
BsaBI GATNNNNATC 1 cut(s) 517
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 972, 984
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 2 cut(s) 802, 1018
Bse118I RCCGGY 1 cut(s) 34
Bse3DI GCAATG 3 cut(s) 806, 843, 879
Bse8I GATNNNNATC 1 cut(s) 517
BseDI CCNNGG 2 cut(s) 972, 984
BseGI GGATG 2 cut(s) 263, 378
BseJI GATNNNNATC 1 cut(s) 517
BseLI CCNNNNNNNGG 2 cut(s) 802, 1018
BseMI GCAATG 3 cut(s) 806, 843, 879
BseRI GAGGAG 2 cut(s) 1031, 1056
BseX3I CGGCCG 1 cut(s) 624
BseXI GCAGC 3 cut(s) 107, 139, 924
Bsh1236I CGCG 2 cut(s) 7, 677
Bsh1285I CGRYCG 4 cut(s) 515, 564, 627, 753
BshFI GGCC 4 cut(s) 626, 823, 983, 1022
BsiEI CGRYCG 4 cut(s) 515, 564, 627, 753
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 1 cut(s) 760
BsiSI CCGG 2 cut(s) 35, 623
BslI CCNNNNNNNGG 2 cut(s) 802, 1018
BsmAI GTCTC 1 cut(s) 152
BsnI GGCC 4 cut(s) 626, 823, 983, 1022
Bso31I GGTCTC 1 cut(s) 152
BsoBI CYCGRG 1 cut(s) 760
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 6 cut(s) 191, 512, 750, 773, 813, 918
BspACI CCGC 5 cut(s) 7, 548, 560, 675, 811
BspANI GGCC 4 cut(s) 626, 823, 983, 1022
BspFNI CGCG 2 cut(s) 7, 677
BspHI TCATGA 1 cut(s) 609
BspLI GGNNCC 3 cut(s) 424, 822, 1065
BspPI GGATC 2 cut(s) 781, 821
BspTNI GGTCTC 1 cut(s) 152
BsrDI GCAATG 3 cut(s) 806, 843, 879
BsrFI RCCGGY 1 cut(s) 34
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 972, 984
BssMI GATC 6 cut(s) 191, 512, 750, 773, 813, 918
BssSI CACGAG 1 cut(s) 1013
BssT1I CCWWGG 2 cut(s) 972, 984
Bst2BI CACGAG 1 cut(s) 1013
Bst4CI ACNGT 5 cut(s) 427, 498, 637, 931, 955
Bst6I CTCTTC 1 cut(s) 207
BstBAI YACGTR 1 cut(s) 801
BstC8I GCNNGC 1 cut(s) 357
BstDEI CTNAG 1 cut(s) 244
BstF5I GGATG 2 cut(s) 263, 378
BstFNI CGCG 2 cut(s) 7, 677
BstHHI GCGC 2 cut(s) 7, 679
BstKTI GATC 6 cut(s) 194, 515, 753, 776, 816, 921
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 6 cut(s) 191, 512, 750, 773, 813, 918
BstMCI CGRYCG 4 cut(s) 515, 564, 627, 753
BstMWI GCNNNNNNNGC 2 cut(s) 651, 1028
BstNSI RCATGY 1 cut(s) 1100
BstUI CGCG 2 cut(s) 7, 677
BstV1I GCAGC 3 cut(s) 107, 139, 924
BstV2I GAAGAC 1 cut(s) 118
BstX2I RGATCY 1 cut(s) 773
BstXI CCANNNNNNTGG 1 cut(s) 979
BstYI RGATCY 1 cut(s) 773
BstZI CGGCCG 1 cut(s) 624
BsuI GTATCC 3 cut(s) 272, 531, 630
BsuRI GGCC 4 cut(s) 626, 823, 983, 1022
BtsCI GGATG 2 cut(s) 263, 378
BtsIMutI CAGTG 1 cut(s) 693
Cac8I GCNNGC 1 cut(s) 357
CciI TCATGA 1 cut(s) 609
CfoI GCGC 2 cut(s) 7, 679
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 470, 672, 821
Csp6I GTAC 1 cut(s) 589
CspCI CAANNNNNGTGG 2 cut(s) 387, 422
CviAII CATG 5 cut(s) 112, 122, 610, 717, 1097
CviQI GTAC 1 cut(s) 589
DdeI CTNAG 1 cut(s) 244
DpnI GATC 6 cut(s) 193, 514, 752, 775, 815, 920
DpnII GATC 6 cut(s) 191, 512, 750, 773, 813, 918
DraI TTTAAA 2 cut(s) 78, 91
EaeI YGGCCR 1 cut(s) 624
EagI CGGCCG 1 cut(s) 624
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
EclXI CGGCCG 1 cut(s) 624
Eco130I CCWWGG 2 cut(s) 972, 984
Eco31I GGTCTC 1 cut(s) 152
Eco47I GGWCC 2 cut(s) 470, 672
Eco52I CGGCCG 1 cut(s) 624
Eco57I CTGAAG 1 cut(s) 717
Eco72I CACGTG 1 cut(s) 801
Eco88I CYCGRG 1 cut(s) 760
EcoO109I RGGNCCY 1 cut(s) 821
EcoT14I CCWWGG 2 cut(s) 972, 984
ErhI CCWWGG 2 cut(s) 972, 984
FaeI CATG 5 cut(s) 115, 125, 613, 720, 1100
FalI AAGNNNNNCTT 2 cut(s) 1020, 1052
FatI CATG 5 cut(s) 111, 121, 609, 716, 1096
FauI CCCGC 1 cut(s) 541
Fnu4HI GCNGC 3 cut(s) 96, 153, 913
FokI GGATG 2 cut(s) 270, 385
Fsp4HI GCNGC 3 cut(s) 96, 153, 913
FspBI CTAG 1 cut(s) 680
GlaI GCGC 2 cut(s) 6, 678
GluI GCNGC 3 cut(s) 96, 153, 913
HaeIII GGCC 4 cut(s) 626, 823, 983, 1022
HapII CCGG 2 cut(s) 35, 623
HhaI GCGC 2 cut(s) 7, 679
Hin1II CATG 5 cut(s) 115, 125, 613, 720, 1100
Hin6I GCGC 2 cut(s) 5, 677
HinP1I GCGC 2 cut(s) 5, 677
HinfI GANTC 4 cut(s) 457, 601, 896, 1040
HpaII CCGG 2 cut(s) 35, 623
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 4 cut(s) 136, 633, 693, 1055
Hpy188I TCNGA 3 cut(s) 21, 191, 600
Hpy188III TCNNGA 4 cut(s) 195, 370, 610, 762
Hpy8I GTNNAC 4 cut(s) 136, 633, 693, 1055
HpyAV CCTTC 4 cut(s) 180, 358, 692, 1054
HpyCH4III ACNGT 5 cut(s) 427, 498, 637, 931, 955
HpyCH4IV ACGT 2 cut(s) 69, 800
HpyCH4V TGCA 9 cut(s) 65, 320, 359, 446, 716, 780, 836, 860, 872
HpyF10VI GCNNNNNNNGC 2 cut(s) 651, 1028
HpyF3I CTNAG 1 cut(s) 244
HpySE526I ACGT 2 cut(s) 69, 800
Hsp92II CATG 5 cut(s) 115, 125, 613, 720, 1100
HspAI GCGC 2 cut(s) 5, 677
Kzo9I GATC 6 cut(s) 191, 512, 750, 773, 813, 918
LmnI GCTCC 1 cut(s) 1069
LpnPI CCDG 7 cut(s) 48, 270, 351, 355, 486, 636, 902
Lsp1109I GCAGC 3 cut(s) 107, 139, 924
LweI GCATC 1 cut(s) 248
MaeI CTAG 1 cut(s) 680
MaeII ACGT 2 cut(s) 69, 800
MaeIII GTNAC 4 cut(s) 37, 454, 682, 1079
MalI GATC 6 cut(s) 193, 514, 752, 775, 815, 920
MboI GATC 6 cut(s) 191, 512, 750, 773, 813, 918
MboII GAAGA 7 cut(s) 118, 155, 158, 194, 733, 877, 1004
MfeI CAATTG 1 cut(s) 861
MflI RGATCY 1 cut(s) 773
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 4 cut(s) 214, 292, 532, 861
MlyI GAGTC 2 cut(s) 451, 595
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 5 cut(s) 341, 434, 1009, 1012, 1077
MseI TTAA 3 cut(s) 77, 90, 390
MslI CAYNNNNRTG 1 cut(s) 956
MspI CCGG 2 cut(s) 35, 623
MssI GTTTAAAC 1 cut(s) 78
MunI CAATTG 1 cut(s) 861
MvnI CGCG 2 cut(s) 7, 677
MwoI GCNNNNNNNGC 2 cut(s) 651, 1028
NdeII GATC 6 cut(s) 191, 512, 750, 773, 813, 918
NlaIII CATG 5 cut(s) 115, 125, 613, 720, 1100
NlaIV GGNNCC 3 cut(s) 424, 822, 1065
NmeAIII GCCGAG 1 cut(s) 652
NmuCI GTSAC 2 cut(s) 454, 682
NspI RCATGY 1 cut(s) 1100
PagI TCATGA 1 cut(s) 609
PciI ACATGT 1 cut(s) 1096
PfeI GAWTC 2 cut(s) 896, 1040
PkrI GCNGC 3 cut(s) 97, 154, 914
Ple19I CGATCG 2 cut(s) 515, 753
PleI GAGTC 2 cut(s) 451, 595
PmaCI CACGTG 1 cut(s) 801
PmeI GTTTAAAC 1 cut(s) 78
PmlI CACGTG 1 cut(s) 801
PpsI GAGTC 2 cut(s) 451, 595
Ppu21I YACGTR 1 cut(s) 801
PscI ACATGT 1 cut(s) 1096
PsiI TTATAA 1 cut(s) 465
Psp1406I AACGTT 1 cut(s) 69
PspCI CACGTG 1 cut(s) 801
PspN4I GGNNCC 3 cut(s) 424, 822, 1065
PspPI GGNCC 3 cut(s) 470, 672, 821
PsuI RGATCY 1 cut(s) 773
PvuI CGATCG 2 cut(s) 515, 753
RgaI GCGATCGC 1 cut(s) 515
RsaI GTAC 1 cut(s) 590
RsaNI GTAC 1 cut(s) 589
RseI CAYNNNNRTG 1 cut(s) 956
SaqAI TTAA 3 cut(s) 77, 90, 390
SatI GCNGC 3 cut(s) 96, 153, 913
Sau3AI GATC 6 cut(s) 191, 512, 750, 773, 813, 918
Sau96I GGNCC 3 cut(s) 470, 672, 821
SchI GAGTC 2 cut(s) 451, 595
SduI GDGCHC 1 cut(s) 243
SfaAI GCGATCGC 1 cut(s) 515
SfaNI GCATC 1 cut(s) 248
SgfI GCGATCGC 1 cut(s) 515
SinI GGWCC 2 cut(s) 470, 672
SmiMI CAYNNNNRTG 1 cut(s) 956
Sse9I AATT 4 cut(s) 214, 292, 532, 861
SsiI CCGC 5 cut(s) 7, 548, 560, 675, 811
SspMI CTAG 1 cut(s) 680
StyI CCWWGG 2 cut(s) 972, 984
TaaI ACNGT 5 cut(s) 427, 498, 637, 931, 955
TaiI ACGT 2 cut(s) 72, 803
TaqI TCGA 4 cut(s) 521, 564, 749, 990
TasI AATT 4 cut(s) 214, 292, 532, 861
TfiI GAWTC 2 cut(s) 896, 1040
Tru1I TTAA 3 cut(s) 77, 90, 390
Tru9I TTAA 3 cut(s) 77, 90, 390
TscAI CASTG 1 cut(s) 700
TseFI GTSAC 2 cut(s) 454, 682
TseI GCWGC 3 cut(s) 95, 152, 912
Tsp45I GTSAC 2 cut(s) 454, 682
TspDTI ATGAA 4 cut(s) 110, 118, 227, 1041
TspRI CASTG 1 cut(s) 700
VpaK11BI GGWCC 2 cut(s) 470, 672
XapI RAATTY 1 cut(s) 214
XceI RCATGY 1 cut(s) 1100
XspI CTAG 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.