Rw2G051630

WAT1-related protein At3g28050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004267
Physical Location & Seq
Reverse (-)
79304208 .. 79306376
2169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G051630.1

Sequence Viewer

Length: 867 bp
ATGGTGACTATGGAGTCCCTGGACGTGGGCTTGAACACACTGTTCAAAGCTGCAAAAATGACAGGAATGAGCTACCATGTCTTTATTGCCTACACTTACTCCATAGCTGCTCTTGCTCTTGTTCCAGCTCCTTTCATCTCCCACAGGCTTCCTCCCCTCAACTTTTCCATTTTGAGCAAAATTCTTCTTCTTGGGCTAATTGGGAGCTCATCTCAGATAATGGGGTATGCAGGAATCAATTACAGCTCTCCAATGCTTTATTCTGCTATCAGCAACCTTCTGCCAGCTTTCACTTTCATTCTTGCCATCATTTTCAGGTTGGAAACTGTAGCATTGAAAACCAGAAGCACTCAAGCTAAAATTGTTGGTACTATAGTTTCACTCGCAGGTGCATTTGTGGCACAAATTATGAAAGAGTACCCAAATGAATTAACTGTGATCTTCTTCTACAATGTTGTGGTCAGCATCGTAGCTGTAGTTGTTGGTTTGATTACAGAAAAAAATCCAAATGCTTGGAGATTGAGACCTGATATGGCATTGGTATCCATCCTGTGCTCAGGACTACTAAACTCATTATTGAACAATACCGTTCACACATGGACCTTGCGCTTGAAGGGACCTGTGTATGTGACAATGTTCAAGCCATTGTCGATTGCCATTGCTGTTGTCATGGGCGTAACGTTCCTTGGTGATACACTTCATCTGGGAAGCCTACTTGGAGCAACAATTATATCAGTTGGGTTTTACACGGTACTGTGGGGAAAAGCTAAAGAAGAGACTTGTGAAGACTCTGGAGCTGATAGCCTGGAATCACCATCTAGAAATAAGGCCCCCTTATTGCAAACCTACAAAACGGAGAATGTCTAG

Protein Analysis

288

Amino Acids

31.23

Weight (kDa)

9.13

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 126 - 252 8.4e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 377
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 377
AclI AACGTT 1 cut(s) 680
AcsI RAATTY 1 cut(s) 180
AfaI GTAC 3 cut(s) 370, 419, 753
AfiI CCNNNNNNNGG 1 cut(s) 25
AgsI TTSAA 6 cut(s) 34, 46, 337, 580, 613, 640
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 2 cut(s) 18, 804
AjuI GAANNNNNNNTTGG 2 cut(s) 244, 276
Alw21I GWGCWC 2 cut(s) 209, 557
Alw26I GTCTC 2 cut(s) 517, 770
AoxI GGCC 1 cut(s) 828
ApeKI GCWGC 2 cut(s) 50, 107
ApoI RAATTY 1 cut(s) 180
AspLEI GCGC 1 cut(s) 609
AspS9I GGNCC 3 cut(s) 600, 617, 829
AsuHPI GGTGA 3 cut(s) 16, 701, 804
AvaII GGWCC 2 cut(s) 600, 617
BaeI ACNNNNGTAYC 2 cut(s) 360, 393
BanII GRGCYC 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 792
Bbv12I GWGCWC 2 cut(s) 209, 557
BbvI GCAGC 2 cut(s) 37, 94
BccI CCATC 3 cut(s) 314, 554, 823
BciT130I CCWGG 2 cut(s) 20, 806
BciVI GTATCC 1 cut(s) 553
BcoDI GTCTC 2 cut(s) 517, 770
BfaI CTAG 2 cut(s) 819, 865
BfmI CTRYAG 3 cut(s) 327, 372, 474
BfuAI ACCTGC 1 cut(s) 377
BfuI GTATCC 1 cut(s) 553
BisI GCNGC 2 cut(s) 51, 108
BlsI GCNGC 2 cut(s) 52, 109
Bme1390I CCNGG 2 cut(s) 20, 806
Bme18I GGWCC 2 cut(s) 600, 617
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 3 cut(s) 600, 617, 829
BmiI GGNNCC 2 cut(s) 618, 831
BmrFI CCNGG 2 cut(s) 20, 806
BmsI GCATC 1 cut(s) 474
BpiI GAAGAC 1 cut(s) 792
BplI GAGNNNNNCTC 2 cut(s) 196, 228
BpmI CTGGAG 1 cut(s) 813
Bpu10I CCTNAGC 1 cut(s) 556
BpuEI CTTGAG 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 517
BsaJI CCNNGG 2 cut(s) 18, 685
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 1 cut(s) 25
Bse3DI GCAATG 1 cut(s) 657
BseBI CCWGG 2 cut(s) 20, 806
BseDI CCNNGG 2 cut(s) 18, 685
BseGI GGATG 1 cut(s) 546
BseLI CCNNNNNNNGG 1 cut(s) 25
BseMI GCAATG 1 cut(s) 657
BseMII CTCAG 2 cut(s) 227, 570
BseXI GCAGC 2 cut(s) 37, 94
BshFI GGCC 1 cut(s) 830
BsiHKAI GWGCWC 2 cut(s) 209, 557
BslFI GGGAC 1 cut(s) 630
BslI CCNNNNNNNGG 1 cut(s) 25
BsmAI GTCTC 2 cut(s) 517, 770
BsmFI GGGAC 1 cut(s) 630
BsnI GGCC 1 cut(s) 830
Bso31I GGTCTC 1 cut(s) 517
Bsp1286I GDGCHC 2 cut(s) 209, 557
Bsp143I GATC 1 cut(s) 438
BspANI GGCC 1 cut(s) 830
BspCNI CTCAG 2 cut(s) 226, 569
BspLI GGNNCC 2 cut(s) 618, 831
BspMI ACCTGC 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 517
BsrDI GCAATG 1 cut(s) 657
BssECI CCNNGG 2 cut(s) 18, 685
BssMI GATC 1 cut(s) 438
BssT1I CCWWGG 1 cut(s) 685
Bst2UI CCWGG 2 cut(s) 20, 806
Bst4CI ACNGT 6 cut(s) 42, 328, 436, 589, 751, 756
Bst6I CTCTTC 1 cut(s) 768
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 2 cut(s) 213, 556
BstF5I GGATG 1 cut(s) 546
BstHHI GCGC 1 cut(s) 609
BstKTI GATC 1 cut(s) 441
BstMAI GTCTC 2 cut(s) 517, 770
BstMBI GATC 1 cut(s) 438
BstMWI GCNNNNNNNGC 2 cut(s) 113, 398
BstNI CCWGG 2 cut(s) 20, 806
BstSCI CCNGG 2 cut(s) 18, 804
BstSFI CTRYAG 3 cut(s) 327, 372, 474
BstV1I GCAGC 2 cut(s) 37, 94
BstV2I GAAGAC 1 cut(s) 792
BstXI CCANNNNNNTGG 1 cut(s) 513
BsuI GTATCC 1 cut(s) 553
BsuRI GGCC 1 cut(s) 830
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 1 cut(s) 546
BtsIMutI CAGTG 1 cut(s) 38
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 1 cut(s) 285
CfoI GCGC 1 cut(s) 609
Cfr13I GGNCC 3 cut(s) 600, 617, 829
Csp6I GTAC 3 cut(s) 369, 418, 752
CviAII CATG 3 cut(s) 77, 597, 670
CviQI GTAC 3 cut(s) 369, 418, 752
DdeI CTNAG 2 cut(s) 213, 556
DpnI GATC 1 cut(s) 440
DpnII GATC 1 cut(s) 438
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Ecl136II GAGCTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 685
Eco24I GRGCYC 1 cut(s) 209
Eco31I GGTCTC 1 cut(s) 517
Eco47I GGWCC 2 cut(s) 600, 617
Eco53kI GAGCTC 1 cut(s) 207
EcoICRI GAGCTC 1 cut(s) 207
EcoO109I RGGNCCY 2 cut(s) 617, 829
EcoRII CCWGG 2 cut(s) 18, 804
EcoT14I CCWWGG 1 cut(s) 685
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 685
FaeI CATG 3 cut(s) 80, 600, 673
FalI AAGNNNNNCTT 2 cut(s) 818, 850
FaqI GGGAC 1 cut(s) 630
FatI CATG 3 cut(s) 76, 596, 669
Fnu4HI GCNGC 2 cut(s) 51, 108
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 2 cut(s) 51, 108
FspBI CTAG 2 cut(s) 819, 865
GlaI GCGC 1 cut(s) 608
GluI GCNGC 2 cut(s) 51, 108
GsuI CTGGAG 1 cut(s) 813
HaeIII GGCC 1 cut(s) 830
HhaI GCGC 1 cut(s) 609
Hin1II CATG 3 cut(s) 80, 600, 673
Hin6I GCGC 1 cut(s) 607
HinP1I GCGC 1 cut(s) 607
HinfI GANTC 4 cut(s) 14, 234, 788, 809
HphI GGTGA 3 cut(s) 16, 701, 804
Hpy166II GTNNAC 1 cut(s) 592
Hpy188I TCNGA 1 cut(s) 216
Hpy188III TCNNGA 3 cut(s) 558, 792, 819
Hpy8I GTNNAC 1 cut(s) 592
HpyAV CCTTC 2 cut(s) 287, 607
HpyCH4III ACNGT 6 cut(s) 42, 328, 436, 589, 751, 756
HpyCH4IV ACGT 2 cut(s) 24, 680
HpyCH4V TGCA 4 cut(s) 53, 230, 392, 841
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 398
HpyF3I CTNAG 2 cut(s) 213, 556
HpySE526I ACGT 2 cut(s) 24, 680
Hsp92II CATG 3 cut(s) 80, 600, 673
HspAI GCGC 1 cut(s) 607
Kzo9I GATC 1 cut(s) 438
LmnI GCTCC 4 cut(s) 133, 204, 719, 794
Lsp1109I GCAGC 2 cut(s) 37, 94
LweI GCATC 1 cut(s) 474
MaeI CTAG 2 cut(s) 819, 865
MaeII ACGT 2 cut(s) 24, 680
MaeIII GTNAC 3 cut(s) 4, 628, 676
MalI GATC 1 cut(s) 440
MboI GATC 1 cut(s) 438
MboII GAAGA 6 cut(s) 176, 179, 433, 436, 785, 797
MhlI GDGCHC 2 cut(s) 209, 557
MluCI AATT 7 cut(s) 180, 198, 238, 360, 405, 428, 726
MlyI GAGTC 2 cut(s) 23, 782
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 2 cut(s) 162, 167
MseI TTAA 1 cut(s) 431
MspR9I CCNGG 2 cut(s) 20, 806
MvaI CCWGG 2 cut(s) 20, 806
MwoI GCNNNNNNNGC 2 cut(s) 113, 398
NdeII GATC 1 cut(s) 438
NlaIII CATG 3 cut(s) 80, 600, 673
NlaIV GGNNCC 2 cut(s) 618, 831
NmuCI GTSAC 2 cut(s) 4, 628
PaqCI CACCTGC 1 cut(s) 377
PfeI GAWTC 2 cut(s) 234, 809
PkrI GCNGC 2 cut(s) 52, 109
PleI GAGTC 2 cut(s) 22, 782
PpsI GAGTC 2 cut(s) 22, 782
PpuMI RGGWCCY 1 cut(s) 617
Psp124BI GAGCTC 1 cut(s) 209
Psp1406I AACGTT 1 cut(s) 680
Psp5II RGGWCCY 1 cut(s) 617
Psp6I CCWGG 2 cut(s) 18, 804
PspGI CCWGG 2 cut(s) 18, 804
PspN4I GGNNCC 2 cut(s) 618, 831
PspPI GGNCC 3 cut(s) 600, 617, 829
PspPPI RGGWCCY 1 cut(s) 617
RsaI GTAC 3 cut(s) 370, 419, 753
RsaNI GTAC 3 cut(s) 369, 418, 752
SacI GAGCTC 1 cut(s) 209
SaqAI TTAA 1 cut(s) 431
SatI GCNGC 2 cut(s) 51, 108
Sau3AI GATC 1 cut(s) 438
Sau96I GGNCC 3 cut(s) 600, 617, 829
SchI GAGTC 2 cut(s) 23, 782
ScrFI CCNGG 2 cut(s) 20, 806
SduI GDGCHC 2 cut(s) 209, 557
SfaNI GCATC 1 cut(s) 474
SfcI CTRYAG 3 cut(s) 327, 372, 474
SinI GGWCC 2 cut(s) 600, 617
SmlI CTYRAG 1 cut(s) 351
SmoI CTYRAG 1 cut(s) 351
Sse9I AATT 7 cut(s) 180, 198, 238, 360, 405, 428, 726
SspMI CTAG 2 cut(s) 819, 865
SstI GAGCTC 1 cut(s) 209
StyD4I CCNGG 2 cut(s) 18, 804
StyI CCWWGG 1 cut(s) 685
TaaI ACNGT 6 cut(s) 42, 328, 436, 589, 751, 756
TaiI ACGT 2 cut(s) 27, 683
TaqI TCGA 1 cut(s) 650
TasI AATT 7 cut(s) 180, 198, 238, 360, 405, 428, 726
TfiI GAWTC 2 cut(s) 234, 809
Tru1I TTAA 1 cut(s) 431
Tru9I TTAA 1 cut(s) 431
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 2 cut(s) 4, 628
TseI GCWGC 2 cut(s) 50, 107
Tsp45I GTSAC 2 cut(s) 4, 628
TspDTI ATGAA 5 cut(s) 124, 286, 425, 441, 689
TspRI CASTG 1 cut(s) 45
VpaK11BI GGWCC 2 cut(s) 600, 617
XapI RAATTY 1 cut(s) 180
XbaI TCTAGA 1 cut(s) 818
XspI CTAG 2 cut(s) 819, 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.