Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004267

Physical Location & Seq

Genomic Coordinates Forward (+)

2261937 .. 2265410

Gene Structure 3474 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1036900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGGCAGCAGTAGGGAGGTCTTTTTACAGTGATGTGCTGCCATTCACGGCGATGGTGACGATGGAGTGCGTGAACGTCGGCCTAAACACTCTCTTCAAAGCTGCCACTTTAAATGGAATGAGCTACCATGTCTTTGTTGTCTACTCTTACTCTATTGCTGCCTTTGTTCTCATTCCAGCTCCTTTCATCTCCCACAGATCAAGGGTGCTTCCTCCTCTCAACTTCTCCATAATGTCTAAAGTTCTTCTTCTTGGACTCATAGGGAGTTCATCACAGATCATGGGGTACACAGGAATCAACTACAGCTCTCCAACGCTTGCTTCAGCCATCAGCAATCTTGTGCCAGCTTTCACTTTCATCCTCGCCATCATTTTCAGGTTGGAAAGTGCAGCTCTAAGAAAGAGAAGCAGTCAAGCAAAAATCTTGGGTACAGTAGTTTCACTTGCAGGTGCATTTGTGGTGACTCTCTACAAGGGCCCTCCAATTGTGTTTGCTAATCAGTCACAATCCCTTTCACTCCACCAGCCTCTTCTAAACTCTACACCAACAAACCCAAATTGGATAGTTGGTGGCCTTCTGCTAACGGCTGAGTACATATTGGTACCACTTTGGTACATTGTTCAGGCACAAATCATGAAAGAGTACCCGAATGAGTTAACCGTGATCTTCTTCTACAATGTATGTGTGGTTGCCGTATCTGCAGCGGTTGCTTTGATAACAGAACCGAATTCCAGCGCTTGGAAGTTGCGACCTGATATTGCATTGCTCTCCGTACTCTGCTCAGGGCTATTTGGGTCATTTTTGAACAATGCTGTTCACACATGGGTGTTGCGCTTGAAGGGACCTGTCTTTGTGACAATGTTCAAGCCTTTGTCCATGGCCATTGCCGTTGCCATGGGCGTTGCTTTCCTCCGTGACACTCTTCATCTCGGAAGCCTAGTTGGAGCAACAATAATATCTATTGGGTTTTATACTGTACTGTGGGGAAAAACAAAGGAAGAGGCCGGTGAAGAATCTGTGCCTGCTAGCCTGGAATCCCCATCAACCACCCACAAGGCCCCTTTGTTGCAAAACTACAAAAACGAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005938 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009987 GO:0010033 GO:0016020 GO:0016021 GO:0023052 GO:0031224 GO:0031226 GO:0032870 GO:0042221 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

364

Amino Acids

39.29

Weight (kDa)

9.35

Isoelectric Point (pI)

41.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	26 - 155	2.3e-08	EamA-like transporter family
EamA	PF00892	205 - 327	9.6e-07	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004267)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G28050 AT3G28060
fragaria_vesca	FvH4_6g50360 FvH4_6g50360 FvH4_6g50360 FvH4_6g50360 FvH4_6g50360
malus_domestica	MD09G1036900.v1.1 MD17G1038300.v1.1
prunus_persica	Prupe.3G280700_v2.0.a1 Prupe.3G280700_v2.0.a1 Prupe.3G280700_v2.0.a1
pyrus_communis	pycom111g02970 pycom17g03450
rosa_chinensis	RchiOBHm_Chr2g0170681 RchiOBHm_Chr2g0170701
rosa_laevigata	RLG00000021975 RLG00000021976
rosa_multiflora	Rmu_sc0003787.1_g000008 Rmu_sc0003787.1_g000009
rosa_roxburghii	Rroxscaffold_2G00081110 Rroxscaffold_2G00081120
rosa_rugosa	Rorug02G0550400 Rorug02G0550500
rosa_samantha	Rh2AG623400 Rh2AG623500 Rh2BG633900 Rh2BG634000 Rh2CG603100 Rh2CG603200
rosa_wichuraiana	Rw2G051630 Rw2G051640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	437
Acc36I	ACCTGC	1 cut(s)	437
Acc65I	GGTACC	1 cut(s)	601
AccB1I	GGYRCC	1 cut(s)	601
AccB7I	CCANNNNNTGG	1 cut(s)	738
AccI	GTMKAC	1 cut(s)	141
AciI	CCGC	1 cut(s)	704
AcoI	YGGCCR	1 cut(s)	879
AcsI	RAATTY	1 cut(s)	727
AcuI	CTGAAG	1 cut(s)	306
AfaI	GTAC	8 cut(s)	287, 430, 593, 603, 614, 644, 774, 978
AfeI	AGCGCT	1 cut(s)	736
AfiI	CCNNNNNNNGG	1 cut(s)	738
AgsI	TTSAA	4 cut(s)	97, 805, 838, 865
AjnI	CCWGG	1 cut(s)	1029
AjuI	GAANNNNNNNTTGG	2 cut(s)	304, 336
AleI	CACNNNNGTG	1 cut(s)	824
AluBI	AGCT	6 cut(s)	101, 123, 179, 306, 347, 392
AluI	AGCT	6 cut(s)	101, 123, 179, 306, 347, 392
Aor51HI	AGCGCT	1 cut(s)	736
AoxI	GGCC	6 cut(s)	79, 475, 571, 879, 1002, 1056
ApaI	GGGCCC	1 cut(s)	479
ApeKI	GCWGC	6 cut(s)	5, 37, 101, 158, 389, 701
ApoI	RAATTY	1 cut(s)	727
Asp718I	GGTACC	1 cut(s)	601
AspLEI	GCGC	2 cut(s)	737, 834
AspS9I	GGNCC	4 cut(s)	475, 476, 842, 1057
AsuHPI	GGTGA	3 cut(s)	67, 472, 1019
AsuNHI	GCTAGC	1 cut(s)	1025
AvaII	GGWCC	1 cut(s)	842
BaeGI	GKGCMC	1 cut(s)	479
BaeI	ACNNNNGTAYC	2 cut(s)	420, 453
BalI	TGGCCA	1 cut(s)	881
BanI	GGYRCC	1 cut(s)	601
BanII	GRGCYC	1 cut(s)	479
BbvI	GCAGC	6 cut(s)	17, 24, 88, 145, 401, 713
BccI	CCATC	5 cut(s)	46, 55, 335, 374, 1048
BceAI	ACGGC	4 cut(s)	63, 600, 677, 872
BciT130I	CCWGG	1 cut(s)	1031
BfaI	CTAG	2 cut(s)	938, 1026
BfmI	CTRYAG	2 cut(s)	301, 699
BfoI	RGCGCY	1 cut(s)	738
BfuAI	ACCTGC	1 cut(s)	437
BisI	GCNGC	6 cut(s)	6, 38, 102, 159, 390, 702
BlsI	GCNGC	6 cut(s)	7, 39, 103, 160, 391, 703
Bme1390I	CCNGG	1 cut(s)	1031
Bme18I	GGWCC	1 cut(s)	842
BmgT120I	GGNCC	4 cut(s)	475, 476, 842, 1057
BmiI	GGNNCC	4 cut(s)	477, 603, 843, 1059
BmrFI	CCNGG	1 cut(s)	1031
BmtI	GCTAGC	1 cut(s)	1029
Bpu10I	CCTNAGC	1 cut(s)	781
BsaJI	CCNNGG	2 cut(s)	876, 894
Bsc4I	CCNNNNNNNGG	1 cut(s)	738
Bse118I	RCCGGY	1 cut(s)	1004
Bse3DI	GCAATG	2 cut(s)	761, 882
BseBI	CCWGG	1 cut(s)	1031
BseDI	CCNNGG	2 cut(s)	876, 894
BseGI	GGATG	1 cut(s)	357
BseLI	CCNNNNNNNGG	1 cut(s)	738
BseMI	GCAATG	2 cut(s)	761, 882
BseMII	CTCAG	2 cut(s)	579, 795
BseRI	GAGGAG	1 cut(s)	204
BseSI	GKGCMC	1 cut(s)	479
BseXI	GCAGC	6 cut(s)	17, 24, 88, 145, 401, 713
BsgI	GTGCAG	1 cut(s)	408
BshFI	GGCC	6 cut(s)	81, 477, 573, 881, 1004, 1058
BshNI	GGYRCC	1 cut(s)	601
BsiSI	CCGG	1 cut(s)	1005
BslFI	GGGAC	1 cut(s)	855
BslI	CCNNNNNNNGG	1 cut(s)	738
BsmFI	GGGAC	1 cut(s)	855
BsnI	GGCC	6 cut(s)	81, 477, 573, 881, 1004, 1058
Bsp120I	GGGCCC	1 cut(s)	475
Bsp1286I	GDGCHC	1 cut(s)	479
Bsp143I	GATC	3 cut(s)	197, 276, 663
Bsp19I	CCATGG	2 cut(s)	876, 894
BspACI	CCGC	1 cut(s)	704
BspANI	GGCC	6 cut(s)	81, 477, 573, 881, 1004, 1058
BspCNI	CTCAG	2 cut(s)	580, 794
BspHI	TCATGA	1 cut(s)	633
BspLI	GGNNCC	4 cut(s)	477, 603, 843, 1059
BspMAI	CTGCAG	1 cut(s)	703
BspMI	ACCTGC	1 cut(s)	437
BspOI	GCTAGC	1 cut(s)	1029
BspT107I	GGYRCC	1 cut(s)	601
BsrDI	GCAATG	2 cut(s)	761, 882
BsrFI	RCCGGY	1 cut(s)	1004
BssAI	RCCGGY	1 cut(s)	1004
BssECI	CCNNGG	2 cut(s)	876, 894
BssMI	GATC	3 cut(s)	197, 276, 663
BssT1I	CCWWGG	2 cut(s)	876, 894
Bst2UI	CCWGG	1 cut(s)	1031
Bst4CI	ACNGT	5 cut(s)	29, 433, 661, 976, 981
Bst6I	CTCTTC	4 cut(s)	98, 534, 927, 993
BstAPI	GCANNNNNTGC	1 cut(s)	707
BstC8I	GCNNGC	4 cut(s)	318, 345, 1023, 1027
BstDEI	CTNAG	3 cut(s)	395, 588, 781
BstDSI	CCRYGG	2 cut(s)	876, 894
BstF5I	GGATG	1 cut(s)	357
BstH2I	RGCGCY	1 cut(s)	738
BstHHI	GCGC	2 cut(s)	737, 834
BstKTI	GATC	3 cut(s)	200, 279, 666
BstMBI	GATC	3 cut(s)	197, 276, 663
BstMWI	GCNNNNNNNGC	2 cut(s)	698, 707
BstNI	CCWGG	1 cut(s)	1031
BstSCI	CCNGG	1 cut(s)	1029
BstSFI	CTRYAG	2 cut(s)	301, 699
BstSLI	GKGCMC	1 cut(s)	479
BstV1I	GCAGC	6 cut(s)	17, 24, 88, 145, 401, 713
BsuRI	GGCC	6 cut(s)	81, 477, 573, 881, 1004, 1058
BtgI	CCRYGG	2 cut(s)	876, 894
BtgZI	GCGATG	1 cut(s)	65
BtsCI	GGATG	1 cut(s)	357
BtsIMutI	CAGTG	1 cut(s)	34
BveI	ACCTGC	1 cut(s)	437
Cac8I	GCNNGC	4 cut(s)	318, 345, 1023, 1027
CciI	TCATGA	1 cut(s)	633
CfoI	GCGC	2 cut(s)	737, 834
Cfr10I	RCCGGY	1 cut(s)	1004
Cfr13I	GGNCC	4 cut(s)	475, 476, 842, 1057
Csp6I	GTAC	8 cut(s)	286, 429, 592, 602, 613, 643, 773, 977
CviAII	CATG	6 cut(s)	128, 280, 634, 822, 877, 895
CviQI	GTAC	8 cut(s)	286, 429, 592, 602, 613, 643, 773, 977
DdeI	CTNAG	3 cut(s)	395, 588, 781
DpnI	GATC	3 cut(s)	199, 278, 665
DpnII	GATC	3 cut(s)	197, 276, 663
DraI	TTTAAA	1 cut(s)	111
EaeI	YGGCCR	1 cut(s)	879
Eam1104I	CTCTTC	4 cut(s)	98, 534, 927, 993
EarI	CTCTTC	4 cut(s)	98, 534, 927, 993
Eco130I	CCWWGG	2 cut(s)	876, 894
Eco24I	GRGCYC	1 cut(s)	479
Eco47I	GGWCC	1 cut(s)	842
Eco47III	AGCGCT	1 cut(s)	736
Eco57I	CTGAAG	1 cut(s)	306
EcoO109I	RGGNCCY	4 cut(s)	475, 476, 842, 1057
EcoRI	GAATTC	1 cut(s)	727
EcoRII	CCWGG	1 cut(s)	1029
EcoT14I	CCWWGG	2 cut(s)	876, 894
EcoT38I	GRGCYC	1 cut(s)	479
ErhI	CCWWGG	2 cut(s)	876, 894
FaeI	CATG	6 cut(s)	131, 283, 637, 825, 880, 898
FalI	AAGNNNNNCTT	2 cut(s)	231, 263
FaqI	GGGAC	1 cut(s)	855
FatI	CATG	6 cut(s)	127, 279, 633, 821, 876, 894
FblI	GTMKAC	1 cut(s)	141
Fnu4HI	GCNGC	6 cut(s)	6, 38, 102, 159, 390, 702
FokI	GGATG	1 cut(s)	344
FriOI	GRGCYC	1 cut(s)	479
Fsp4HI	GCNGC	6 cut(s)	6, 38, 102, 159, 390, 702
FspBI	CTAG	2 cut(s)	938, 1026
GlaI	GCGC	2 cut(s)	736, 833
GluI	GCNGC	6 cut(s)	6, 38, 102, 159, 390, 702
HaeII	RGCGCY	1 cut(s)	738
HaeIII	GGCC	6 cut(s)	81, 477, 573, 881, 1004, 1058
HapII	CCGG	1 cut(s)	1005
HhaI	GCGC	2 cut(s)	737, 834
Hin1II	CATG	6 cut(s)	131, 283, 637, 825, 880, 898
Hin6I	GCGC	2 cut(s)	735, 832
HinP1I	GCGC	2 cut(s)	735, 832
HincII	GTYRAC	1 cut(s)	657
HindII	GTYRAC	1 cut(s)	657
HinfI	GANTC	5 cut(s)	255, 294, 463, 1013, 1034
HpaI	GTTAAC	1 cut(s)	657
HpaII	CCGG	1 cut(s)	1005
HphI	GGTGA	3 cut(s)	67, 472, 1019
Hpy166II	GTNNAC	5 cut(s)	73, 142, 288, 657, 817
Hpy188I	TCNGA	1 cut(s)	932
Hpy188III	TCNNGA	1 cut(s)	634
Hpy8I	GTNNAC	5 cut(s)	73, 142, 288, 657, 817
Hpy99I	CGWCG	1 cut(s)	80
HpyAV	CCTTC	2 cut(s)	584, 832
HpyCH4III	ACNGT	5 cut(s)	29, 433, 661, 976, 981
HpyCH4IV	ACGT	1 cut(s)	75
HpyCH4V	TGCA	6 cut(s)	389, 446, 452, 701, 761, 1069
HpyF10VI	GCNNNNNNNGC	2 cut(s)	698, 707
HpyF3I	CTNAG	3 cut(s)	395, 588, 781
HpySE526I	ACGT	1 cut(s)	75
Hsp92II	CATG	6 cut(s)	131, 283, 637, 825, 880, 898
HspAI	GCGC	2 cut(s)	735, 832
KpnI	GGTACC	1 cut(s)	605
KspAI	GTTAAC	1 cut(s)	657
Kzo9I	GATC	3 cut(s)	197, 276, 663
LmnI	GCTCC	2 cut(s)	184, 944
Lsp1109I	GCAGC	6 cut(s)	17, 24, 88, 145, 401, 713
MaeI	CTAG	2 cut(s)	938, 1026
MaeII	ACGT	1 cut(s)	75
MaeIII	GTNAC	5 cut(s)	55, 460, 501, 853, 914
MalI	GATC	3 cut(s)	199, 278, 665
MboI	GATC	3 cut(s)	197, 276, 663
MboII	GAAGA	9 cut(s)	85, 236, 239, 521, 658, 661, 914, 1010, 1022
MfeI	CAATTG	1 cut(s)	483
MhlI	GDGCHC	1 cut(s)	479
MlsI	TGGCCA	1 cut(s)	881
MluCI	AATT	3 cut(s)	483, 556, 727
MluNI	TGGCCA	1 cut(s)	881
MlyI	GAGTC	2 cut(s)	249, 457
MmeI	TCCRAC	3 cut(s)	335, 360, 922
MnlI	CCTC	8 cut(s)	9, 222, 225, 371, 489, 537, 920, 994
Mox20I	TGGCCA	1 cut(s)	881
MscI	TGGCCA	1 cut(s)	881
MseI	TTAA	2 cut(s)	110, 656
MslI	CAYNNNNRTG	2 cut(s)	50, 824
Msp20I	TGGCCA	1 cut(s)	881
MspA1I	CMGCKG	1 cut(s)	704
MspI	CCGG	1 cut(s)	1005
MspR9I	CCNGG	1 cut(s)	1031
MunI	CAATTG	1 cut(s)	483
MvaI	CCWGG	1 cut(s)	1031
MwoI	GCNNNNNNNGC	2 cut(s)	698, 707
NcoI	CCATGG	2 cut(s)	876, 894
NdeII	GATC	3 cut(s)	197, 276, 663
NheI	GCTAGC	1 cut(s)	1025
NlaIII	CATG	6 cut(s)	131, 283, 637, 825, 880, 898
NlaIV	GGNNCC	4 cut(s)	477, 603, 843, 1059
NmuCI	GTSAC	5 cut(s)	55, 460, 501, 853, 914
OliI	CACNNNNGTG	1 cut(s)	824
PagI	TCATGA	1 cut(s)	633
PaqCI	CACCTGC	1 cut(s)	437
PfeI	GAWTC	3 cut(s)	294, 1013, 1034
PflMI	CCANNNNNTGG	1 cut(s)	738
PkrI	GCNGC	6 cut(s)	7, 39, 103, 160, 391, 703
PleI	GAGTC	2 cut(s)	249, 457
PpsI	GAGTC	2 cut(s)	249, 457
PpuMI	RGGWCCY	1 cut(s)	842
Psp5II	RGGWCCY	1 cut(s)	842
Psp6I	CCWGG	1 cut(s)	1029
PspGI	CCWGG	1 cut(s)	1029
PspN4I	GGNNCC	4 cut(s)	477, 603, 843, 1059
PspOMI	GGGCCC	1 cut(s)	475
PspPI	GGNCC	4 cut(s)	475, 476, 842, 1057
PspPPI	RGGWCCY	1 cut(s)	842
PstI	CTGCAG	1 cut(s)	703
RsaI	GTAC	8 cut(s)	287, 430, 593, 603, 614, 644, 774, 978
RsaNI	GTAC	8 cut(s)	286, 429, 592, 602, 613, 643, 773, 977
RseI	CAYNNNNRTG	2 cut(s)	50, 824
SaqAI	TTAA	2 cut(s)	110, 656
SatI	GCNGC	6 cut(s)	6, 38, 102, 159, 390, 702
Sau3AI	GATC	3 cut(s)	197, 276, 663
Sau96I	GGNCC	4 cut(s)	475, 476, 842, 1057
SchI	GAGTC	2 cut(s)	249, 457
ScrFI	CCNGG	1 cut(s)	1031
SduI	GDGCHC	1 cut(s)	479
SfcI	CTRYAG	2 cut(s)	301, 699
SinI	GGWCC	1 cut(s)	842
SmiMI	CAYNNNNRTG	2 cut(s)	50, 824
Sse9I	AATT	3 cut(s)	483, 556, 727
SsiI	CCGC	1 cut(s)	704
SspMI	CTAG	2 cut(s)	938, 1026
StyD4I	CCNGG	1 cut(s)	1029
StyI	CCWWGG	2 cut(s)	876, 894
TaaI	ACNGT	5 cut(s)	29, 433, 661, 976, 981
TaiI	ACGT	1 cut(s)	78
TasI	AATT	3 cut(s)	483, 556, 727
TatI	WGTACW	2 cut(s)	591, 976
TfiI	GAWTC	3 cut(s)	294, 1013, 1034
Tru1I	TTAA	2 cut(s)	110, 656
Tru9I	TTAA	2 cut(s)	110, 656
TscAI	CASTG	1 cut(s)	34
TseFI	GTSAC	5 cut(s)	55, 460, 501, 853, 914
TseI	GCWGC	6 cut(s)	5, 37, 101, 158, 389, 701
Tsp45I	GTSAC	5 cut(s)	55, 460, 501, 853, 914
TspDTI	ATGAA	5 cut(s)	175, 258, 346, 650, 914
TspGWI	ACGGA	2 cut(s)	760, 902
TspRI	CASTG	1 cut(s)	34
Van91I	CCANNNNNTGG	1 cut(s)	738
VpaK11BI	GGWCC	1 cut(s)	842
XapI	RAATTY	1 cut(s)	727
XmiI	GTMKAC	1 cut(s)	141
XspI	CTAG	2 cut(s)	938, 1026

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.