RchiOBHm_Chr2g0170681

WAT1-related protein At3g28050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004267
Physical Location & Seq
Reverse (-)
84542141 .. 84543482
1342 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53817

Sequence Viewer

Length: 717 bp
ATGTATAATTTTGCCTGGTTGGAAACTGTAGCATTGAAAACCAGAAGCACTCAAGCTAAAATTGTTGGTACTATAGTTTCACTCGCAGGTGCATTTGTGGTGACTCTATATAAGGGCTCCCGAATTTCCCTCACTGGCCGAACCCAATCTCTTTTGCTAAACTCAACACCTTCAAAATCAAACTGGGTCATTGGAGGCCTTCTGATAACAGCCGAGTATATATTGGTGACACTTTGGTACATTGTTCTGGCACAAATTATGAAAGAGTACCCAAATGAATTAACTGTGATCTTCTTCTACAATGTTGTGGTCAGCATCGTAGCTGTAGTTGTTGGTTTGATTACAGAAAAAAATCCAAATGCTTGGAGATTGAGACCTGATATGGCATTGGTATCCATCCTGTGCTCAGGACTACTAAACTCATTATTGAACAATACCGTTCACACATGGACCTTGCGCTTGAAGGGACCTGTGTATGTGACAATGTTCAAGCCATTGTCGATTGCCATTGCTGTTGTCATGGGCGTAACGTTCCTTGGTGATACACTTCATCTGGGAAGCCTACTTGGAGCAACAATTATATCAGTTGGGTTTTACACGGTACTGTGGGGAAAAGCTAAAGAAGAGACTTGTGAAGACTCTGGAGCTGATAGCCTGGAATCACCATCTAGAAATAAGGCCCCCTTATTGCAAACCTACAAAACGGAGAATGTCTAG

Protein Analysis

238

Amino Acids

26.08

Weight (kDa)

9.18

Isoelectric Point (pI)

26.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 80 - 202 1.7e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 77
AclI AACGTT 1 cut(s) 530
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 1 cut(s) 123
AfaI GTAC 4 cut(s) 70, 239, 269, 603
AgsI TTSAA 5 cut(s) 37, 174, 430, 463, 490
AjnI CCWGG 2 cut(s) 14, 654
AluBI AGCT 4 cut(s) 56, 323, 617, 647
AluI AGCT 4 cut(s) 56, 323, 617, 647
Alw21I GWGCWC 1 cut(s) 407
Alw26I GTCTC 2 cut(s) 367, 620
AoxI GGCC 3 cut(s) 136, 196, 678
ApoI RAATTY 1 cut(s) 123
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 3 cut(s) 450, 467, 679
AsuHPI GGTGA 4 cut(s) 112, 238, 551, 654
AvaII GGWCC 2 cut(s) 450, 467
BaeI ACNNNNGTAYC 2 cut(s) 60, 93
BanII GRGCYC 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 642
Bbv12I GWGCWC 1 cut(s) 407
BccI CCATC 2 cut(s) 404, 673
BciT130I CCWGG 2 cut(s) 16, 656
BciVI GTATCC 1 cut(s) 403
BcoDI GTCTC 2 cut(s) 367, 620
BfaI CTAG 2 cut(s) 669, 715
BfmI CTRYAG 3 cut(s) 27, 72, 324
BfuAI ACCTGC 1 cut(s) 77
BfuI GTATCC 1 cut(s) 403
Bme1390I CCNGG 2 cut(s) 16, 656
Bme18I GGWCC 2 cut(s) 450, 467
BmgT120I GGNCC 3 cut(s) 450, 467, 679
BmiI GGNNCC 3 cut(s) 118, 468, 681
BmrFI CCNGG 2 cut(s) 16, 656
BmrI ACTGGG 1 cut(s) 193
BmsI GCATC 1 cut(s) 324
BmuI ACTGGG 1 cut(s) 193
BpiI GAAGAC 1 cut(s) 642
BpmI CTGGAG 1 cut(s) 663
Bpu10I CCTNAGC 1 cut(s) 406
BpuEI CTTGAG 1 cut(s) 36
BsaI GGTCTC 1 cut(s) 367
BsaJI CCNNGG 1 cut(s) 535
Bse1I ACTGG 2 cut(s) 139, 188
Bse3DI GCAATG 1 cut(s) 507
BseBI CCWGG 2 cut(s) 16, 656
BseDI CCNNGG 1 cut(s) 535
BseGI GGATG 1 cut(s) 396
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 2 cut(s) 139, 188
BshFI GGCC 3 cut(s) 138, 198, 680
BsiHKAI GWGCWC 1 cut(s) 407
BslFI GGGAC 1 cut(s) 480
BsmAI GTCTC 2 cut(s) 367, 620
BsmFI GGGAC 1 cut(s) 480
BsnI GGCC 3 cut(s) 138, 198, 680
Bso31I GGTCTC 1 cut(s) 367
Bsp1286I GDGCHC 2 cut(s) 119, 407
Bsp143I GATC 1 cut(s) 288
BspANI GGCC 3 cut(s) 138, 198, 680
BspCNI CTCAG 1 cut(s) 419
BspLI GGNNCC 3 cut(s) 118, 468, 681
BspMI ACCTGC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 367
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 2 cut(s) 139, 188
BssECI CCNNGG 1 cut(s) 535
BssMI GATC 1 cut(s) 288
BssT1I CCWWGG 1 cut(s) 535
Bst2UI CCWGG 2 cut(s) 16, 656
Bst4CI ACNGT 5 cut(s) 28, 286, 439, 601, 606
Bst6I CTCTTC 1 cut(s) 618
BstDEI CTNAG 1 cut(s) 406
BstF5I GGATG 1 cut(s) 396
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 1 cut(s) 291
BstMAI GTCTC 2 cut(s) 367, 620
BstMBI GATC 1 cut(s) 288
BstNI CCWGG 2 cut(s) 16, 656
BstSCI CCNGG 2 cut(s) 14, 654
BstSFI CTRYAG 3 cut(s) 27, 72, 324
BstV2I GAAGAC 1 cut(s) 642
BstXI CCANNNNNNTGG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 3 cut(s) 138, 198, 680
BtsCI GGATG 1 cut(s) 396
BtsIMutI CAGTG 1 cut(s) 132
BveI ACCTGC 1 cut(s) 77
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 3 cut(s) 450, 467, 679
Csp6I GTAC 4 cut(s) 69, 238, 268, 602
CviAII CATG 2 cut(s) 447, 520
CviQI GTAC 4 cut(s) 69, 238, 268, 602
DdeI CTNAG 1 cut(s) 406
DpnI GATC 1 cut(s) 290
DpnII GATC 1 cut(s) 288
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Eco130I CCWWGG 1 cut(s) 535
Eco147I AGGCCT 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 119
Eco31I GGTCTC 1 cut(s) 367
Eco47I GGWCC 2 cut(s) 450, 467
EcoO109I RGGNCCY 2 cut(s) 467, 679
EcoRII CCWGG 2 cut(s) 14, 654
EcoT14I CCWWGG 1 cut(s) 535
EcoT38I GRGCYC 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 2 cut(s) 450, 523
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FaqI GGGAC 1 cut(s) 480
FatI CATG 2 cut(s) 446, 519
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 1 cut(s) 119
FspBI CTAG 2 cut(s) 669, 715
GlaI GCGC 1 cut(s) 458
GsuI CTGGAG 1 cut(s) 663
HaeIII GGCC 3 cut(s) 138, 198, 680
HhaI GCGC 1 cut(s) 459
Hin1II CATG 2 cut(s) 450, 523
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HinfI GANTC 3 cut(s) 103, 638, 659
HphI GGTGA 4 cut(s) 112, 238, 551, 654
Hpy166II GTNNAC 1 cut(s) 442
Hpy188I TCNGA 1 cut(s) 204
Hpy188III TCNNGA 4 cut(s) 120, 408, 642, 669
Hpy8I GTNNAC 1 cut(s) 442
HpyAV CCTTC 3 cut(s) 180, 209, 457
HpyCH4III ACNGT 5 cut(s) 28, 286, 439, 601, 606
HpyCH4IV ACGT 1 cut(s) 530
HpyCH4V TGCA 2 cut(s) 92, 691
HpyF3I CTNAG 1 cut(s) 406
HpySE526I ACGT 1 cut(s) 530
Hsp92II CATG 2 cut(s) 450, 523
HspAI GCGC 1 cut(s) 457
Kzo9I GATC 1 cut(s) 288
LmnI GCTCC 3 cut(s) 122, 569, 644
LweI GCATC 1 cut(s) 324
MaeI CTAG 2 cut(s) 669, 715
MaeII ACGT 1 cut(s) 530
MaeIII GTNAC 4 cut(s) 100, 226, 478, 526
MalI GATC 1 cut(s) 290
MboI GATC 1 cut(s) 288
MboII GAAGA 4 cut(s) 283, 286, 635, 647
MhlI GDGCHC 2 cut(s) 119, 407
MluCI AATT 6 cut(s) 7, 60, 123, 255, 278, 576
MlyI GAGTC 2 cut(s) 97, 632
MnlI CCTC 2 cut(s) 140, 188
MseI TTAA 1 cut(s) 281
MspR9I CCNGG 2 cut(s) 16, 656
MvaI CCWGG 2 cut(s) 16, 656
NdeII GATC 1 cut(s) 288
NlaIII CATG 2 cut(s) 450, 523
NlaIV GGNNCC 3 cut(s) 118, 468, 681
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 3 cut(s) 100, 226, 478
PaqCI CACCTGC 1 cut(s) 77
PceI AGGCCT 1 cut(s) 198
PfeI GAWTC 1 cut(s) 659
PleI GAGTC 2 cut(s) 97, 632
PpsI GAGTC 2 cut(s) 97, 632
PpuMI RGGWCCY 1 cut(s) 467
Psp1406I AACGTT 1 cut(s) 530
Psp5II RGGWCCY 1 cut(s) 467
Psp6I CCWGG 2 cut(s) 14, 654
PspGI CCWGG 2 cut(s) 14, 654
PspN4I GGNNCC 3 cut(s) 118, 468, 681
PspPI GGNCC 3 cut(s) 450, 467, 679
PspPPI RGGWCCY 1 cut(s) 467
RsaI GTAC 4 cut(s) 70, 239, 269, 603
RsaNI GTAC 4 cut(s) 69, 238, 268, 602
SaqAI TTAA 1 cut(s) 281
Sau3AI GATC 1 cut(s) 288
Sau96I GGNCC 3 cut(s) 450, 467, 679
SchI GAGTC 2 cut(s) 97, 632
ScrFI CCNGG 2 cut(s) 16, 656
SduI GDGCHC 2 cut(s) 119, 407
SfaNI GCATC 1 cut(s) 324
SfcI CTRYAG 3 cut(s) 27, 72, 324
SinI GGWCC 2 cut(s) 450, 467
SmlI CTYRAG 1 cut(s) 51
SmoI CTYRAG 1 cut(s) 51
Sse9I AATT 6 cut(s) 7, 60, 123, 255, 278, 576
SseBI AGGCCT 1 cut(s) 198
SspMI CTAG 2 cut(s) 669, 715
StuI AGGCCT 1 cut(s) 198
StyD4I CCNGG 2 cut(s) 14, 654
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 5 cut(s) 28, 286, 439, 601, 606
TaiI ACGT 1 cut(s) 533
TaqI TCGA 1 cut(s) 500
TasI AATT 6 cut(s) 7, 60, 123, 255, 278, 576
TfiI GAWTC 1 cut(s) 659
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 3 cut(s) 100, 226, 478
Tsp45I GTSAC 3 cut(s) 100, 226, 478
TspDTI ATGAA 3 cut(s) 275, 291, 539
TspRI CASTG 1 cut(s) 139
VpaK11BI GGWCC 2 cut(s) 450, 467
XapI RAATTY 1 cut(s) 123
XbaI TCTAGA 1 cut(s) 668
XspI CTAG 2 cut(s) 669, 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.