RLG00000021975

WAT1-related protein At3g28050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004267
Physical Location & Seq
Reverse (-)
79674448 .. 79676409
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021975

Sequence Viewer

Length: 1002 bp
ATGGCAGGAAGGTCTTGTTACAGAGATGTCCTGCCATTTACAGCCATGGTGACTATGGAGTCCATGGACGTGGGCTTGAACACACTGTTTAAAGCTGCAACAATGACAGGAATGAGCTACCATGTCTTTATTGCCTACACTTACTCCATAGCTGCTCTTGCTCTTGTTCCGGCTCCTTTCATCTCCCACAGATCAAGGAGGCTTCCTTCTCTCAACTTTTCCATTATGAGCAAAATCCTTCTTCTTGGGCTAATTGGGGAAGGAATCCAATATATGTTAGTTGTACTTTCCCTAGCTACTTTGTTGGAAACTGTAGCATTGAAAACCAGAAGCACTCAAGCTAAAATTGTTGGTACTATAGTTTCACTCGCAGGTGCATTTGTGGTGACTCTATATAACGGCCCCCCAATTTCCCTCACTGGCCGAACCCAATCGCTTTTGCTAAACTCAACACCTTCAGACTCAAACTGGGTCATTGGAGGCCTTCTGATAACAGCCGAGTATATATTGGTGACACTTTGGTACATTGTTCTGGCACAAATTATGAAAGAGTACCCAAATGAATTAACTGTGATCTTCTTCTACAATGTTGTGGTCAGCATCGTAGTTGTAGTTGTTGGTTTGATTACAGAAAAAAATCCAAATGCTTGGAGATTGAGACCTGATATGGCATTGGTATCCATCCTGTGCTCAGGACTACTAAACTCATTATTGAACAATACCGTTCACACATGGACCTTGCGCTTGAAGGGACCTGTGTATGTGACAATGTTCAAGCCATTGTCGATTGCCATTGCTGTTGTCATGGGCATAACGTTCCTTGGTGATACACTTCATCTGGGAAGCCTCCTTGGATCAACAATTATATCAGTTGGGTTTTACACGGTACTGTGGGCAAAAGATAAAGAAGAGACCTGTGAAGACTCTGGAGCTGATAGCCTGGAATCACCATCTAGCAATAAGGCCCCTTTATTGCAAACCTATAAAACCGAGACTGTCTAG

Protein Analysis

334

Amino Acids

36.29

Weight (kDa)

8.33

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 175 - 297 2.7e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 362
AasI GACNNNNNNGTC 1 cut(s) 58
Acc36I ACCTGC 1 cut(s) 362
AclI AACGTT 1 cut(s) 815
AclWI GGATC 1 cut(s) 862
AcoI YGGCCR 1 cut(s) 421
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 5 cut(s) 285, 355, 524, 554, 888
AgsI TTSAA 5 cut(s) 79, 322, 715, 748, 775
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 939
AluBI AGCT 6 cut(s) 95, 117, 152, 296, 341, 932
AluI AGCT 6 cut(s) 95, 117, 152, 296, 341, 932
Alw21I GWGCWC 1 cut(s) 692
Alw26I GTCTC 3 cut(s) 652, 905, 986
AlwI GGATC 1 cut(s) 862
AoxI GGCC 4 cut(s) 400, 421, 481, 963
ApeKI GCWGC 2 cut(s) 95, 152
AspLEI GCGC 1 cut(s) 744
AspS9I GGNCC 4 cut(s) 401, 735, 752, 964
AsuHPI GGTGA 5 cut(s) 61, 397, 523, 836, 939
AvaII GGWCC 2 cut(s) 735, 752
BaeI ACNNNNGTAYC 2 cut(s) 345, 378
BbsI GAAGAC 1 cut(s) 927
Bbv12I GWGCWC 1 cut(s) 692
BbvI GCAGC 2 cut(s) 82, 139
BccI CCATC 2 cut(s) 689, 958
BceAI ACGGC 1 cut(s) 415
BciT130I CCWGG 1 cut(s) 941
BciVI GTATCC 1 cut(s) 688
BcoDI GTCTC 3 cut(s) 652, 905, 986
BfaI CTAG 3 cut(s) 293, 954, 1000
BfmI CTRYAG 2 cut(s) 312, 357
BfuAI ACCTGC 1 cut(s) 362
BfuI GTATCC 1 cut(s) 688
BisI GCNGC 2 cut(s) 96, 153
BlsI GCNGC 2 cut(s) 97, 154
Bme1390I CCNGG 1 cut(s) 941
Bme18I GGWCC 2 cut(s) 735, 752
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 4 cut(s) 401, 735, 752, 964
BmiI GGNNCC 4 cut(s) 174, 403, 753, 966
BmrFI CCNGG 1 cut(s) 941
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 1 cut(s) 609
BmuI ACTGGG 1 cut(s) 478
BpiI GAAGAC 1 cut(s) 927
BpmI CTGGAG 1 cut(s) 948
Bpu10I CCTNAGC 1 cut(s) 691
BpuEI CTTGAG 1 cut(s) 321
BsaI GGTCTC 2 cut(s) 652, 905
BsaJI CCNNGG 4 cut(s) 45, 63, 820, 850
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bse1I ACTGG 2 cut(s) 424, 473
Bse3DI GCAATG 1 cut(s) 792
BseBI CCWGG 1 cut(s) 941
BseDI CCNNGG 4 cut(s) 45, 63, 820, 850
BseGI GGATG 1 cut(s) 681
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 1 cut(s) 705
BseNI ACTGG 2 cut(s) 424, 473
BseXI GCAGC 2 cut(s) 82, 139
BshFI GGCC 4 cut(s) 402, 423, 483, 965
BsiHKAI GWGCWC 1 cut(s) 692
BsiSI CCGG 1 cut(s) 170
BslFI GGGAC 1 cut(s) 765
BsmAI GTCTC 3 cut(s) 652, 905, 986
BsmFI GGGAC 1 cut(s) 765
BsnI GGCC 4 cut(s) 402, 423, 483, 965
Bso31I GGTCTC 2 cut(s) 652, 905
Bsp1286I GDGCHC 1 cut(s) 692
Bsp143I GATC 3 cut(s) 191, 573, 854
Bsp19I CCATGG 2 cut(s) 45, 63
BspANI GGCC 4 cut(s) 402, 423, 483, 965
BspCNI CTCAG 1 cut(s) 704
BspLI GGNNCC 4 cut(s) 174, 403, 753, 966
BspMI ACCTGC 1 cut(s) 362
BspPI GGATC 1 cut(s) 862
BspTNI GGTCTC 2 cut(s) 652, 905
BsrDI GCAATG 1 cut(s) 792
BsrI ACTGG 2 cut(s) 424, 473
BssECI CCNNGG 4 cut(s) 45, 63, 820, 850
BssMI GATC 3 cut(s) 191, 573, 854
BssT1I CCWWGG 4 cut(s) 45, 63, 820, 850
Bst2UI CCWGG 1 cut(s) 941
Bst4CI ACNGT 7 cut(s) 87, 313, 571, 724, 886, 891, 997
Bst6I CTCTTC 1 cut(s) 903
BstDEI CTNAG 1 cut(s) 691
BstDSI CCRYGG 2 cut(s) 45, 63
BstF5I GGATG 1 cut(s) 681
BstHHI GCGC 1 cut(s) 744
BstKTI GATC 3 cut(s) 194, 576, 857
BstMAI GTCTC 3 cut(s) 652, 905, 986
BstMBI GATC 3 cut(s) 191, 573, 854
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstNI CCWGG 1 cut(s) 941
BstSCI CCNGG 1 cut(s) 939
BstSFI CTRYAG 2 cut(s) 312, 357
BstV1I GCAGC 2 cut(s) 82, 139
BstV2I GAAGAC 1 cut(s) 927
BstXI CCANNNNNNTGG 2 cut(s) 70, 648
BsuI GTATCC 1 cut(s) 688
BsuRI GGCC 4 cut(s) 402, 423, 483, 965
BtgI CCRYGG 2 cut(s) 45, 63
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 1 cut(s) 681
BtsIMutI CAGTG 2 cut(s) 83, 417
BveI ACCTGC 1 cut(s) 362
CfoI GCGC 1 cut(s) 744
Cfr13I GGNCC 4 cut(s) 401, 735, 752, 964
Csp6I GTAC 5 cut(s) 284, 354, 523, 553, 887
CviAII CATG 5 cut(s) 46, 64, 122, 732, 805
CviQI GTAC 5 cut(s) 284, 354, 523, 553, 887
DdeI CTNAG 1 cut(s) 691
DpnI GATC 3 cut(s) 193, 575, 856
DpnII GATC 3 cut(s) 191, 573, 854
DraI TTTAAA 1 cut(s) 91
DrdI GACNNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 1 cut(s) 58
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 903
EarI CTCTTC 1 cut(s) 903
Eco130I CCWWGG 4 cut(s) 45, 63, 820, 850
Eco147I AGGCCT 1 cut(s) 483
Eco31I GGTCTC 2 cut(s) 652, 905
Eco47I GGWCC 2 cut(s) 735, 752
Eco57I CTGAAG 1 cut(s) 441
EcoO109I RGGNCCY 2 cut(s) 752, 964
EcoRII CCWGG 1 cut(s) 939
EcoT14I CCWWGG 4 cut(s) 45, 63, 820, 850
ErhI CCWWGG 4 cut(s) 45, 63, 820, 850
FaeI CATG 5 cut(s) 49, 67, 125, 735, 808
FaqI GGGAC 1 cut(s) 765
FatI CATG 5 cut(s) 45, 63, 121, 731, 804
Fnu4HI GCNGC 2 cut(s) 96, 153
FokI GGATG 1 cut(s) 668
Fsp4HI GCNGC 2 cut(s) 96, 153
FspBI CTAG 3 cut(s) 293, 954, 1000
GlaI GCGC 1 cut(s) 743
GluI GCNGC 2 cut(s) 96, 153
GsuI CTGGAG 1 cut(s) 948
HaeIII GGCC 4 cut(s) 402, 423, 483, 965
HapII CCGG 1 cut(s) 170
HhaI GCGC 1 cut(s) 744
Hin1II CATG 5 cut(s) 49, 67, 125, 735, 808
Hin6I GCGC 1 cut(s) 742
HinP1I GCGC 1 cut(s) 742
HinfI GANTC 6 cut(s) 59, 264, 388, 461, 923, 944
HpaII CCGG 1 cut(s) 170
HphI GGTGA 5 cut(s) 61, 397, 523, 836, 939
Hpy166II GTNNAC 1 cut(s) 727
Hpy188I TCNGA 2 cut(s) 460, 489
Hpy188III TCNNGA 2 cut(s) 693, 927
Hpy8I GTNNAC 1 cut(s) 727
HpyAV CCTTC 7 cut(s) 3, 216, 248, 254, 465, 494, 742
HpyCH4III ACNGT 7 cut(s) 87, 313, 571, 724, 886, 891, 997
HpyCH4IV ACGT 2 cut(s) 69, 815
HpyCH4V TGCA 3 cut(s) 98, 377, 976
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 1 cut(s) 691
HpySE526I ACGT 2 cut(s) 69, 815
Hsp92II CATG 5 cut(s) 49, 67, 125, 735, 808
HspAI GCGC 1 cut(s) 742
Kzo9I GATC 3 cut(s) 191, 573, 854
LmnI GCTCC 2 cut(s) 178, 929
Lsp1109I GCAGC 2 cut(s) 82, 139
LweI GCATC 1 cut(s) 609
MaeI CTAG 3 cut(s) 293, 954, 1000
MaeII ACGT 2 cut(s) 69, 815
MaeIII GTNAC 5 cut(s) 17, 49, 385, 511, 763
MalI GATC 3 cut(s) 193, 575, 856
MboI GATC 3 cut(s) 191, 573, 854
MboII GAAGA 5 cut(s) 233, 568, 571, 920, 932
MhlI GDGCHC 1 cut(s) 692
MluCI AATT 6 cut(s) 252, 345, 408, 540, 563, 861
MlyI GAGTC 4 cut(s) 68, 382, 455, 917
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 4 cut(s) 192, 425, 473, 857
MseI TTAA 2 cut(s) 90, 566
MslI CAYNNNNRTG 1 cut(s) 68
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 941
MvaI CCWGG 1 cut(s) 941
MwoI GCNNNNNNNGC 1 cut(s) 158
NcoI CCATGG 2 cut(s) 45, 63
NdeII GATC 3 cut(s) 191, 573, 854
NlaIII CATG 5 cut(s) 49, 67, 125, 735, 808
NlaIV GGNNCC 4 cut(s) 174, 403, 753, 966
NmeAIII GCCGAG 1 cut(s) 523
NmuCI GTSAC 4 cut(s) 49, 385, 511, 763
PaqCI CACCTGC 1 cut(s) 362
PceI AGGCCT 1 cut(s) 483
PfeI GAWTC 2 cut(s) 264, 944
PkrI GCNGC 2 cut(s) 97, 154
PleI GAGTC 4 cut(s) 67, 382, 455, 917
PpsI GAGTC 4 cut(s) 67, 382, 455, 917
PpuMI RGGWCCY 1 cut(s) 752
Psp1406I AACGTT 1 cut(s) 815
Psp5II RGGWCCY 1 cut(s) 752
Psp6I CCWGG 1 cut(s) 939
PspGI CCWGG 1 cut(s) 939
PspN4I GGNNCC 4 cut(s) 174, 403, 753, 966
PspPI GGNCC 4 cut(s) 401, 735, 752, 964
PspPPI RGGWCCY 1 cut(s) 752
RsaI GTAC 5 cut(s) 285, 355, 524, 554, 888
RsaNI GTAC 5 cut(s) 284, 354, 523, 553, 887
RseI CAYNNNNRTG 1 cut(s) 68
SaqAI TTAA 2 cut(s) 90, 566
SatI GCNGC 2 cut(s) 96, 153
Sau3AI GATC 3 cut(s) 191, 573, 854
Sau96I GGNCC 4 cut(s) 401, 735, 752, 964
SchI GAGTC 4 cut(s) 68, 382, 455, 917
ScrFI CCNGG 1 cut(s) 941
SduI GDGCHC 1 cut(s) 692
SfaNI GCATC 1 cut(s) 609
SfcI CTRYAG 2 cut(s) 312, 357
SinI GGWCC 2 cut(s) 735, 752
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 336
SmoI CTYRAG 1 cut(s) 336
Sse9I AATT 6 cut(s) 252, 345, 408, 540, 563, 861
SseBI AGGCCT 1 cut(s) 483
SspMI CTAG 3 cut(s) 293, 954, 1000
StuI AGGCCT 1 cut(s) 483
StyD4I CCNGG 1 cut(s) 939
StyI CCWWGG 4 cut(s) 45, 63, 820, 850
TaaI ACNGT 7 cut(s) 87, 313, 571, 724, 886, 891, 997
TaiI ACGT 2 cut(s) 72, 818
TaqI TCGA 1 cut(s) 785
TasI AATT 6 cut(s) 252, 345, 408, 540, 563, 861
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 2 cut(s) 264, 944
Tru1I TTAA 2 cut(s) 90, 566
Tru9I TTAA 2 cut(s) 90, 566
TscAI CASTG 2 cut(s) 90, 424
TseFI GTSAC 4 cut(s) 49, 385, 511, 763
TseI GCWGC 2 cut(s) 95, 152
Tsp45I GTSAC 4 cut(s) 49, 385, 511, 763
TspDTI ATGAA 4 cut(s) 169, 560, 576, 824
TspRI CASTG 2 cut(s) 90, 424
VpaK11BI GGWCC 2 cut(s) 735, 752
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XspI CTAG 3 cut(s) 293, 954, 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.