Rw2G051640

WAT1-related protein At3g28050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004267
Physical Location & Seq
Reverse (-)
79307184 .. 79309057
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G051640.1

Sequence Viewer

Length: 990 bp
ATGGCAGCAGGAAGGTCTTGTTACAGAGATGTGCTTCCGTTTACAGCCATGGTGGCTATGGAGTCCACCAACGTGGGATTGAACACACTGTTCAAAGCTGCAACCATGAGAGGGATGAGCTACCATGTCTTTATTGTCTACTCTTATTCCATAGCTGCTCTTGCTCTTCTTCCAGCTCCCTTCATTTCCCACAGATCAAGGAGGCTTCCTCCTCTCAACTTTTCCATCATGAGCAAAATTCTTCTTCTTGGGCTAATTGGGAGTTCATCTCAGATAATGGGGTTTACAGGAATCAGTTACAGCTCTCCAACGCTTGCTTCTGCTATCAGCAACCTTGTGCCAGCTTTCACTTTCATTCTTGCCATTATTTTCAGGATGGAAAGTGTAGCTTTGAAAAGTAGAAGCAGTCAAGCTAAAATCTTGGGTACTATAGTTTCACTTGCAGGTGCATTTGTGGTGACTTTCTATAAGGGCCCCCGAATTTCCCTCACTAGTCAGACACAATCTCTGATGCTAAACTCAACAGCTTCAAACTCAGACTGGATCATTGGAGGCCTTCTAATAACAGCTGAGTATATATTGGTGACACTTTGGTACATTGTTCAGGCACGAATTATGAAAGAGTATCCAAATGAATTAACTGTGATCTTCTTCTACAATGTTATCGTCAGCATCGTAGCTGCAGTTGTTGGTTTGATTACAGAAAAAAATCCAAATGCTTGGAGGTTGAGACCAGATATGGCATTGGTCTCCATCCTGTGCTCAGGATTACTAAGCTCATTATTGAACAATTCTGTTCACACATGGACCTTGCGCGTGAAGGGGCCTGTGTATGTGACAATGTTCAAGCCATTGTCAATTGCCATTGCTGTTGTCATGGGCGTTACGTTCCTTGGTGATACACTTTGCCTGGGAAGCCTACTTGGAGCATCGATAATATCGATTGGGTTTTATACGGTACTATGGGGAAAAGCTAAAGAAGACTGGTGA

Protein Analysis

329

Amino Acids

35.8

Weight (kDa)

9.76

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 26 - 154 7.1e-09 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 434
Acc36I ACCTGC 1 cut(s) 434
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 816
AclWI GGATC 1 cut(s) 551
AcsI RAATTY 2 cut(s) 237, 480
AfaI GTAC 3 cut(s) 427, 596, 960
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 6 cut(s) 82, 94, 394, 531, 787, 847
AhlI ACTAGT 1 cut(s) 491
AjnI CCWGG 1 cut(s) 909
AjuI GAANNNNNNNTTGG 2 cut(s) 301, 333
AleI CACNNNNGTG 1 cut(s) 71
Alw21I GWGCWC 1 cut(s) 764
Alw26I GTCTC 2 cut(s) 724, 754
AlwI GGATC 1 cut(s) 551
AoxI GGCC 3 cut(s) 472, 553, 824
ApaI GGGCCC 1 cut(s) 476
ApeKI GCWGC 4 cut(s) 5, 98, 155, 680
ApoI RAATTY 2 cut(s) 237, 480
AspLEI GCGC 1 cut(s) 816
AspS9I GGNCC 4 cut(s) 472, 473, 807, 824
AsuHPI GGTGA 3 cut(s) 469, 595, 908
AvaII GGWCC 1 cut(s) 807
BaeGI GKGCMC 1 cut(s) 476
BaeI ACNNNNGTAYC 2 cut(s) 417, 450
BanII GRGCYC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 764
BbvI GCAGC 4 cut(s) 17, 85, 142, 667
BccI CCATC 3 cut(s) 233, 370, 761
BciT130I CCWGG 1 cut(s) 911
BciVI GTATCC 1 cut(s) 636
BcoDI GTCTC 2 cut(s) 724, 754
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 1 cut(s) 492
BfmI CTRYAG 2 cut(s) 429, 681
BfuAI ACCTGC 1 cut(s) 434
BfuI GTATCC 1 cut(s) 636
BglI GCCNNNNNGGC 1 cut(s) 53
BisI GCNGC 4 cut(s) 6, 99, 156, 681
BlsI GCNGC 4 cut(s) 7, 100, 157, 682
Bme1390I CCNGG 1 cut(s) 911
Bme18I GGWCC 1 cut(s) 807
BmgT120I GGNCC 4 cut(s) 472, 473, 807, 824
BmiI GGNNCC 3 cut(s) 474, 475, 825
BmrFI CCNGG 1 cut(s) 911
BmsI GCATC 3 cut(s) 501, 681, 938
BplI GAGNNNNNCTC 4 cut(s) 193, 225, 253, 285
Bpu10I CCTNAGC 1 cut(s) 763
Bsa29I ATCGAT 2 cut(s) 932, 941
BsaI GGTCTC 2 cut(s) 724, 754
BsaJI CCNNGG 3 cut(s) 48, 892, 910
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 2 cut(s) 545, 989
Bse3DI GCAATG 1 cut(s) 864
BseBI CCWGG 1 cut(s) 911
BseCI ATCGAT 2 cut(s) 932, 941
BseDI CCNNGG 3 cut(s) 48, 892, 910
BseGI GGATG 3 cut(s) 120, 381, 753
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 4 cut(s) 284, 549, 561, 777
BseNI ACTGG 2 cut(s) 545, 989
BseRI GAGGAG 1 cut(s) 201
BseSI GKGCMC 1 cut(s) 476
BseXI GCAGC 4 cut(s) 17, 85, 142, 667
Bsh1236I CGCG 1 cut(s) 816
BshFI GGCC 3 cut(s) 474, 555, 826
BshVI ATCGAT 2 cut(s) 932, 941
BsiHKAI GWGCWC 1 cut(s) 764
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 724, 754
BsnI GGCC 3 cut(s) 474, 555, 826
Bso31I GGTCTC 2 cut(s) 724, 754
Bsp120I GGGCCC 1 cut(s) 472
Bsp1286I GDGCHC 2 cut(s) 476, 764
Bsp143I GATC 3 cut(s) 194, 543, 645
Bsp19I CCATGG 1 cut(s) 48
BspANI GGCC 3 cut(s) 474, 555, 826
BspCNI CTCAG 4 cut(s) 283, 548, 562, 776
BspDI ATCGAT 2 cut(s) 932, 941
BspFNI CGCG 1 cut(s) 816
BspHI TCATGA 1 cut(s) 228
BspLI GGNNCC 3 cut(s) 474, 475, 825
BspMAI CTGCAG 1 cut(s) 685
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 1 cut(s) 551
BspQI GCTCTTC 1 cut(s) 171
BspTNI GGTCTC 2 cut(s) 724, 754
BsrDI GCAATG 1 cut(s) 864
BsrI ACTGG 2 cut(s) 545, 989
BssECI CCNNGG 3 cut(s) 48, 892, 910
BssMI GATC 3 cut(s) 194, 543, 645
BssT1I CCWWGG 2 cut(s) 48, 892
Bst2UI CCWGG 1 cut(s) 911
Bst4CI ACNGT 3 cut(s) 90, 643, 958
Bst6I CTCTTC 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 315, 342
BstDEI CTNAG 5 cut(s) 270, 535, 570, 763, 773
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 3 cut(s) 120, 381, 753
BstFNI CGCG 1 cut(s) 816
BstHHI GCGC 1 cut(s) 816
BstKTI GATC 3 cut(s) 197, 546, 648
BstMAI GTCTC 2 cut(s) 724, 754
BstMBI GATC 3 cut(s) 194, 543, 645
BstMWI GCNNNNNNNGC 3 cut(s) 53, 161, 915
BstNI CCWGG 1 cut(s) 911
BstSCI CCNGG 1 cut(s) 909
BstSFI CTRYAG 2 cut(s) 429, 681
BstSLI GKGCMC 1 cut(s) 476
BstUI CGCG 1 cut(s) 816
BstV1I GCAGC 4 cut(s) 17, 85, 142, 667
BstXI CCANNNNNNTGG 2 cut(s) 73, 720
Bsu15I ATCGAT 2 cut(s) 932, 941
BsuI GTATCC 1 cut(s) 636
BsuRI GGCC 3 cut(s) 474, 555, 826
BsuTUI ATCGAT 2 cut(s) 932, 941
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 3 cut(s) 120, 381, 753
BtsIMutI CAGTG 1 cut(s) 86
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 2 cut(s) 315, 342
CciI TCATGA 1 cut(s) 228
CfoI GCGC 1 cut(s) 816
Cfr13I GGNCC 4 cut(s) 472, 473, 807, 824
ClaI ATCGAT 2 cut(s) 932, 941
Csp6I GTAC 3 cut(s) 426, 595, 959
CviAII CATG 6 cut(s) 49, 106, 125, 229, 804, 877
CviQI GTAC 3 cut(s) 426, 595, 959
DdeI CTNAG 5 cut(s) 270, 535, 570, 763, 773
DpnI GATC 3 cut(s) 196, 545, 647
DpnII GATC 3 cut(s) 194, 543, 645
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 48, 892
Eco147I AGGCCT 1 cut(s) 555
Eco24I GRGCYC 1 cut(s) 476
Eco31I GGTCTC 2 cut(s) 724, 754
Eco47I GGWCC 1 cut(s) 807
EcoO109I RGGNCCY 3 cut(s) 472, 473, 824
EcoRII CCWGG 1 cut(s) 909
EcoT14I CCWWGG 2 cut(s) 48, 892
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 2 cut(s) 48, 892
FaeI CATG 6 cut(s) 52, 109, 128, 232, 807, 880
FalI AAGNNNNNCTT 2 cut(s) 373, 405
FatI CATG 6 cut(s) 48, 105, 124, 228, 803, 876
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 4 cut(s) 6, 99, 156, 681
FokI GGATG 3 cut(s) 127, 388, 740
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 4 cut(s) 6, 99, 156, 681
FspBI CTAG 1 cut(s) 492
GlaI GCGC 1 cut(s) 815
GluI GCNGC 4 cut(s) 6, 99, 156, 681
HaeIII GGCC 3 cut(s) 474, 555, 826
HhaI GCGC 1 cut(s) 816
Hin1II CATG 6 cut(s) 52, 109, 128, 232, 807, 880
Hin6I GCGC 1 cut(s) 814
HinP1I GCGC 1 cut(s) 814
HinfI GANTC 2 cut(s) 62, 291
HphI GGTGA 3 cut(s) 469, 595, 908
Hpy166II GTNNAC 5 cut(s) 42, 66, 139, 285, 799
Hpy188I TCNGA 4 cut(s) 273, 498, 510, 538
Hpy188III TCNNGA 3 cut(s) 229, 373, 765
Hpy8I GTNNAC 5 cut(s) 42, 66, 139, 285, 799
HpyAV CCTTC 4 cut(s) 6, 190, 566, 814
HpyCH4III ACNGT 3 cut(s) 90, 643, 958
HpyCH4IV ACGT 2 cut(s) 72, 887
HpyCH4V TGCA 4 cut(s) 101, 443, 449, 683
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 161, 915
HpyF3I CTNAG 5 cut(s) 270, 535, 570, 763, 773
HpySE526I ACGT 2 cut(s) 72, 887
Hsp92II CATG 6 cut(s) 52, 109, 128, 232, 807, 880
HspAI GCGC 1 cut(s) 814
Kzo9I GATC 3 cut(s) 194, 543, 645
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 2 cut(s) 181, 926
Lsp1109I GCAGC 4 cut(s) 17, 85, 142, 667
LweI GCATC 3 cut(s) 501, 681, 938
MaeI CTAG 1 cut(s) 492
MaeII ACGT 2 cut(s) 72, 887
MaeIII GTNAC 6 cut(s) 20, 296, 457, 583, 835, 883
MalI GATC 3 cut(s) 196, 545, 647
MboI GATC 3 cut(s) 194, 543, 645
MboII GAAGA 6 cut(s) 158, 161, 233, 236, 640, 643
MfeI CAATTG 1 cut(s) 858
MhlI GDGCHC 2 cut(s) 476, 764
MluCI AATT 7 cut(s) 237, 255, 480, 612, 635, 790, 858
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 332
MnlI CCTC 7 cut(s) 104, 195, 219, 222, 497, 545, 717
MseI TTAA 1 cut(s) 638
MslI CAYNNNNRTG 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 569
MspR9I CCNGG 1 cut(s) 911
MunI CAATTG 1 cut(s) 858
MvaI CCWGG 1 cut(s) 911
MvnI CGCG 1 cut(s) 816
MwoI GCNNNNNNNGC 3 cut(s) 53, 161, 915
NcoI CCATGG 1 cut(s) 48
NdeII GATC 3 cut(s) 194, 543, 645
NlaIII CATG 6 cut(s) 52, 109, 128, 232, 807, 880
NlaIV GGNNCC 3 cut(s) 474, 475, 825
NmuCI GTSAC 3 cut(s) 457, 583, 835
OliI CACNNNNGTG 1 cut(s) 71
PagI TCATGA 1 cut(s) 228
PaqCI CACCTGC 1 cut(s) 434
PceI AGGCCT 1 cut(s) 555
PciSI GCTCTTC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 2 cut(s) 672, 938
PfeI GAWTC 1 cut(s) 291
PkrI GCNGC 4 cut(s) 7, 100, 157, 682
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 909
PspGI CCWGG 1 cut(s) 909
PspN4I GGNNCC 3 cut(s) 474, 475, 825
PspOMI GGGCCC 1 cut(s) 472
PspPI GGNCC 4 cut(s) 472, 473, 807, 824
PstI CTGCAG 1 cut(s) 685
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 3 cut(s) 427, 596, 960
RsaNI GTAC 3 cut(s) 426, 595, 959
RseI CAYNNNNRTG 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 1 cut(s) 638
SatI GCNGC 4 cut(s) 6, 99, 156, 681
Sau3AI GATC 3 cut(s) 194, 543, 645
Sau96I GGNCC 4 cut(s) 472, 473, 807, 824
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 911
SduI GDGCHC 2 cut(s) 476, 764
SfaNI GCATC 3 cut(s) 501, 681, 938
SfcI CTRYAG 2 cut(s) 429, 681
SinI GGWCC 1 cut(s) 807
SmiMI CAYNNNNRTG 1 cut(s) 71
SpeI ACTAGT 1 cut(s) 491
Sse9I AATT 7 cut(s) 237, 255, 480, 612, 635, 790, 858
SseBI AGGCCT 1 cut(s) 555
SspMI CTAG 1 cut(s) 492
StuI AGGCCT 1 cut(s) 555
StyD4I CCNGG 1 cut(s) 909
StyI CCWWGG 2 cut(s) 48, 892
TaaI ACNGT 3 cut(s) 90, 643, 958
TaiI ACGT 2 cut(s) 75, 890
TaqI TCGA 2 cut(s) 932, 941
TasI AATT 7 cut(s) 237, 255, 480, 612, 635, 790, 858
TfiI GAWTC 1 cut(s) 291
Tru1I TTAA 1 cut(s) 638
Tru9I TTAA 1 cut(s) 638
TscAI CASTG 1 cut(s) 93
TseFI GTSAC 3 cut(s) 457, 583, 835
TseI GCWGC 4 cut(s) 5, 98, 155, 680
Tsp45I GTSAC 3 cut(s) 457, 583, 835
TspDTI ATGAA 5 cut(s) 172, 255, 343, 632, 648
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 1 cut(s) 93
VpaK11BI GGWCC 1 cut(s) 807
XapI RAATTY 2 cut(s) 237, 480
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.