Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004630

Physical Location & Seq

Genomic Coordinates Reverse (-)

21789332 .. 21791476

Gene Structure 2145 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G58970.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1311 bp

ATGGGGAAGGGCCCCTTATCGTTTCGCCGTCTTTCAAGCATCCGTCACCGGAAAAAAGGTTCTGCGGTGAAGGATGACTCCGCACAGACATCGACGCCTTCATCTCCACCGCCTCCCCTACCGATTCATGCTGGCGGATCGGCTGTGGGAGCGACGGGGAAGGCGAAGAAGAAAACCGGAGGAGCTAGGCTGTGGATGAGGTTTGATCGGACTGGTGCTATGGAAGTGGTGGAGTGCGATAAGAGTACAATCATCAAACGTGCTTCTGTTCCCGCTAGGGATTTGAGGATACTTGGCCCTGTCTTCTCTCACTCCTCTAACATTCTCGCCAGAGAGAAAGCTATTGTTGTGAATTTAGAGGTCATAAAGGCAATAGTTACTGCAGAAGAAGTGTTGTTGTTGGATCCTCTCCGTCCTGAAGTTCTTCCATTCGTTGAACGACTAAAGCAACAGTTTCCTCAGAGGAATGGGAATGAAAATGCACTGCAAGCTTCTGCGAATGTACAATCTCCTCTTGATCCAGAAGCTGCAGAGGGTTTGCAGAGTGAGCTTCCTTTTGAGTTTCAGGTCCTGGAGATTGCACTTGAGGTCGTCTGCTCATTTGTTGATAAAAGTGTGGCTGCTCTTGAGACAGAAGCTTGGCCTGTCCTTGATGAACTCACTAAGAATGTCAGCACTGAGAATCTTGAATATGTACGAAGTTTGAAAAGTAATCTCACCCGCTTGCTAGCTCGTGTACAAAAGGTGAGAGATGAACTCGAGCATTTGTTAGATGACAACGAAGACATGGCTGACTTATACTTAACAAGGAAATGGATCCAGAACCAGCAAACCGAGGCAATACTGGCGGGTACAGCATCAAACAGCATTGCCCTCCCAGCACATAATACATCAAATCTTCACCGGCTCACATCAAACAGAAGTGCGAGTATGGTGACGAGCAATACAGAGGAAGATGACGTGGAGGATTTGGAGATGTTGTTGGAGGCATACTTCATGCAACTGGATGGAATGCGGAACAAGATTCTTACAGTGAGGGAGTACATTGACGACACAGAAGACTACGTGAACATTCAACTGGATAATCAACGAAACGAACTGATCCAGCTGCAGCTGACGCTGACCATAGCCTCGTTTGCAATAGCCGCAGAGACATTGTTGGCCAGCTTGTTTGGAATGAACATACCATGTCCATTGTATAGCATACATGGCGTCTTTGGTTACTTTGTGTGGAGTGTTACTGCACTTTGCATTGTGCTTTTCATGGTCACTCTTGGTTACGCCAGGTGGAAGAAGCTGCTAGGCTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0007275 GO:0008150 GO:0008324 GO:0009555 GO:0015075 GO:0015095 GO:0015318 GO:0015693 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0032501 GO:0032502 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0055085 GO:0070838 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1903830

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CorA

Protein Analysis

436

Amino Acids

48.4

Weight (kDa)

5.47

Isoelectric Point (pI)

43.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MRS2-like	PF22099	68 - 278	2.9e-27	Magnesium transporter MRS2-like
CorA	PF01544	304 - 425	3.4e-08	CorA-like Mg2+ transporter protein
MRS2-like	PF22099	314 - 364	3.7e-07	Magnesium transporter MRS2-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004630)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58970
fragaria_vesca	FvH4_5g28740 FvH4_5g28740 FvH4_5g28740 FvH4_5g28740 FvH4_5g28740 FvH4_5g28740
malus_domestica	MD08G1238500.v1.1 MD08G1238700.v1.1 MD15G1430500.v1.1
prunus_persica	Prupe.1G575700_v2.0.a1
pyrus_communis	pycom08g20700 pycom08g20720 pycom15g38020 pycom420g00390 pycom420g00500 pycom420g00530
rosa_chinensis	RchiOBHm_Chr1g0372101 RchiOBHm_Chr6g0246761
rosa_laevigata	RLG00000015250 RLG00000026923
rosa_multiflora	Rmu_sc0001755.1_g000027 Rmu_sc0003131.1_g000033
rosa_roxburghii	Rroxscaffold_4G00285260 Rroxscaffold_7G00214470
rosa_rugosa	Rorug01G0369700
rosa_samantha	Rh1AG378000 Rh1BG341400 Rh1CG354800 Rh1DG372500
rosa_wichuraiana	Rw1G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	9 cut(s)	65, 81, 110, 135, 273, 721, 848, 1015, 1146
AclWI	GGATC	7 cut(s)	145, 398, 411, 512, 811, 824, 1096
AcoI	YGGCCR	1 cut(s)	1161
AcsI	RAATTY	1 cut(s)	352
AcuI	CTGAAG	1 cut(s)	438
AcyI	GRCGYC	2 cut(s)	95, 1212
AfaI	GTAC	6 cut(s)	247, 504, 696, 738, 853, 1043
AgsI	TTSAA	5 cut(s)	36, 437, 689, 706, 1076
AjiI	CACGTC	1 cut(s)	961
AjnI	CCWGG	2 cut(s)	570, 1283
AloI	GAACNNNNNNTCC	2 cut(s)	43, 75
Alw26I	GTCTC	2 cut(s)	623, 1145
AlwI	GGATC	7 cut(s)	145, 398, 411, 512, 811, 824, 1096
AlwNI	CAGNNNCTG	2 cut(s)	527, 571
Ama87I	CYCGRG	1 cut(s)	758
AoxI	GGCC	4 cut(s)	10, 295, 641, 1161
ApaI	GGGCCC	1 cut(s)	14
ApeKI	GCWGC	5 cut(s)	527, 620, 1108, 1111, 1297
ApoI	RAATTY	1 cut(s)	352
Asp700I	GAANNNNTTC	1 cut(s)	423
AspS9I	GGNCC	4 cut(s)	10, 11, 296, 568
AsuHPI	GGTGA	6 cut(s)	38, 79, 709, 757, 893, 946
AsuNHI	GCTAGC	1 cut(s)	727
AvaI	CYCGRG	1 cut(s)	758
AvaII	GGWCC	1 cut(s)	568
BaeGI	GKGCMC	1 cut(s)	14
BalI	TGGCCA	1 cut(s)	1163
BamHI	GGATCC	2 cut(s)	403, 816
BanII	GRGCYC	1 cut(s)	14
BauI	CACGAG	1 cut(s)	732
BbsI	GAAGAC	3 cut(s)	295, 789, 1065
BbvI	GCAGC	5 cut(s)	514, 607, 1095, 1123, 1284
BccI	CCATC	1 cut(s)	1001
BceAI	ACGGC	1 cut(s)	12
BcgI	CGANNNNNNTGC	2 cut(s)	72, 106
BciT130I	CCWGG	2 cut(s)	572, 1285
BciVI	GTATCC	1 cut(s)	282
BcoDI	GTCTC	2 cut(s)	623, 1145
BfaI	CTAG	4 cut(s)	186, 276, 728, 1301
BfmI	CTRYAG	3 cut(s)	381, 528, 1109
BfuI	GTATCC	1 cut(s)	282
BisI	GCNGC	6 cut(s)	528, 621, 1109, 1112, 1146, 1298
BlsI	GCNGC	6 cut(s)	529, 622, 1110, 1113, 1147, 1299
Bme1390I	CCNGG	2 cut(s)	572, 1285
Bme18I	GGWCC	1 cut(s)	568
BmeT110I	CYCGRG	1 cut(s)	758
BmgBI	CACGTC	1 cut(s)	961
BmgT120I	GGNCC	4 cut(s)	10, 11, 296, 568
BmiI	GGNNCC	4 cut(s)	12, 13, 405, 818
BmrFI	CCNGG	2 cut(s)	572, 1285
BmsI	GCATC	2 cut(s)	48, 866
BmtI	GCTAGC	1 cut(s)	731
BpiI	GAAGAC	3 cut(s)	295, 789, 1065
BplI	GAGNNNNNCTC	2 cut(s)	741, 773
BpmI	CTGGAG	1 cut(s)	593
BpuEI	CTTGAG	2 cut(s)	605, 647
BsaAI	YACGTR	1 cut(s)	1066
BsaHI	GRCGYC	2 cut(s)	95, 1212
BsaJI	CCNNGG	1 cut(s)	834
BsaWI	WCCGGW	2 cut(s)	48, 176
BsaXI	ACNNNNNCTCC	2 cut(s)	965, 995
Bse118I	RCCGGY	1 cut(s)	903
Bse1I	ACTGG	4 cut(s)	217, 849, 1008, 1083
Bse3DI	GCAATG	1 cut(s)	867
BseBI	CCWGG	2 cut(s)	572, 1285
BseDI	CCNNGG	1 cut(s)	834
BseGI	GGATG	4 cut(s)	39, 79, 201, 1012
BseMI	GCAATG	1 cut(s)	867
BseMII	CTCAG	2 cut(s)	473, 669
BseNI	ACTGG	4 cut(s)	217, 849, 1008, 1083
BseRI	GAGGAG	3 cut(s)	195, 304, 501
BseSI	GKGCMC	1 cut(s)	14
BseXI	GCAGC	5 cut(s)	514, 607, 1095, 1123, 1284
BseYI	CCCAGC	1 cut(s)	877
BsgI	GTGCAG	1 cut(s)	1227
BshFI	GGCC	4 cut(s)	12, 297, 643, 1163
BsiHKCI	CYCGRG	1 cut(s)	758
BsiSI	CCGG	3 cut(s)	49, 177, 904
BsmAI	GTCTC	2 cut(s)	623, 1145
BsmI	GAATGC	1 cut(s)	1017
BsnI	GGCC	4 cut(s)	12, 297, 643, 1163
BsoBI	CYCGRG	1 cut(s)	758
Bsp120I	GGGCCC	1 cut(s)	10
Bsp1286I	GDGCHC	1 cut(s)	14
Bsp1407I	TGTACA	2 cut(s)	502, 736
Bsp143I	GATC	6 cut(s)	137, 205, 403, 517, 816, 1101
BspACI	CCGC	9 cut(s)	65, 81, 110, 135, 273, 721, 848, 1015, 1146
BspANI	GGCC	4 cut(s)	12, 297, 643, 1163
BspCNI	CTCAG	2 cut(s)	472, 670
BspLI	GGNNCC	4 cut(s)	12, 13, 405, 818
BspMAI	CTGCAG	3 cut(s)	385, 532, 1113
BspOI	GCTAGC	1 cut(s)	731
BspPI	GGATC	7 cut(s)	145, 398, 411, 512, 811, 824, 1096
BsrDI	GCAATG	1 cut(s)	867
BsrFI	RCCGGY	1 cut(s)	903
BsrGI	TGTACA	2 cut(s)	502, 736
BsrI	ACTGG	4 cut(s)	217, 849, 1008, 1083
BssAI	RCCGGY	1 cut(s)	903
BssECI	CCNNGG	1 cut(s)	834
BssMI	GATC	6 cut(s)	137, 205, 403, 517, 816, 1101
BssNI	GRCGYC	2 cut(s)	95, 1212
BssSI	CACGAG	1 cut(s)	732
Bst2BI	CACGAG	1 cut(s)	732
Bst2UI	CCWGG	2 cut(s)	572, 1285
Bst4CI	ACNGT	2 cut(s)	453, 1033
BstACI	GRCGYC	2 cut(s)	95, 1212
BstAUI	TGTACA	2 cut(s)	502, 736
BstBAI	YACGTR	1 cut(s)	1066
BstC8I	GCNNGC	5 cut(s)	133, 489, 725, 729, 1165
BstDEI	CTNAG	3 cut(s)	459, 663, 678
BstF5I	GGATG	4 cut(s)	39, 79, 201, 1012
BstKTI	GATC	6 cut(s)	140, 208, 406, 520, 819, 1104
BstMAI	GTCTC	2 cut(s)	623, 1145
BstMBI	GATC	6 cut(s)	137, 205, 403, 517, 816, 1101
BstNI	CCWGG	2 cut(s)	572, 1285
BstSCI	CCNGG	2 cut(s)	570, 1283
BstSFI	CTRYAG	3 cut(s)	381, 528, 1109
BstSLI	GKGCMC	1 cut(s)	14
BstV1I	GCAGC	5 cut(s)	514, 607, 1095, 1123, 1284
BstV2I	GAAGAC	3 cut(s)	295, 789, 1065
BstX2I	RGATCY	2 cut(s)	403, 816
BstYI	RGATCY	2 cut(s)	403, 816
BsuI	GTATCC	1 cut(s)	282
BsuRI	GGCC	4 cut(s)	12, 297, 643, 1163
BtrI	CACGTC	1 cut(s)	961
BtsCI	GGATG	4 cut(s)	39, 79, 201, 1012
BtsI	GCAGTG	1 cut(s)	482
BtsIMutI	CAGTG	3 cut(s)	482, 675, 1038
Cac8I	GCNNGC	5 cut(s)	133, 489, 725, 729, 1165
CaiI	CAGNNNCTG	2 cut(s)	527, 571
Cfr10I	RCCGGY	1 cut(s)	903
Cfr13I	GGNCC	4 cut(s)	10, 11, 296, 568
CseI	GACGC	3 cut(s)	103, 1126, 1201
Csp6I	GTAC	6 cut(s)	246, 503, 695, 737, 852, 1042
CviAII	CATG	6 cut(s)	128, 787, 997, 1188, 1208, 1264
CviQI	GTAC	6 cut(s)	246, 503, 695, 737, 852, 1042
DdeI	CTNAG	3 cut(s)	459, 663, 678
DpnI	GATC	6 cut(s)	139, 207, 405, 519, 818, 1103
DpnII	GATC	6 cut(s)	137, 205, 403, 517, 816, 1101
EaeI	YGGCCR	1 cut(s)	1161
EciI	GGCGGA	1 cut(s)	150
Eco24I	GRGCYC	1 cut(s)	14
Eco47I	GGWCC	1 cut(s)	568
Eco57I	CTGAAG	1 cut(s)	438
Eco88I	CYCGRG	1 cut(s)	758
EcoO109I	RGGNCCY	3 cut(s)	10, 11, 568
EcoRII	CCWGG	2 cut(s)	570, 1283
EcoT38I	GRGCYC	1 cut(s)	14
FaeI	CATG	6 cut(s)	131, 790, 1000, 1191, 1211, 1267
FalI	AAGNNNNNCTT	1 cut(s)	31
FatI	CATG	6 cut(s)	127, 786, 996, 1187, 1207, 1263
FauI	CCCGC	3 cut(s)	280, 728, 841
Fnu4HI	GCNGC	6 cut(s)	528, 621, 1109, 1112, 1146, 1298
FokI	GGATG	4 cut(s)	26, 86, 208, 1019
FriOI	GRGCYC	1 cut(s)	14
Fsp4HI	GCNGC	6 cut(s)	528, 621, 1109, 1112, 1146, 1298
FspBI	CTAG	4 cut(s)	186, 276, 728, 1301
GluI	GCNGC	6 cut(s)	528, 621, 1109, 1112, 1146, 1298
GsaI	CCCAGC	1 cut(s)	881
GsuI	CTGGAG	1 cut(s)	593
HaeIII	GGCC	4 cut(s)	12, 297, 643, 1163
HapII	CCGG	3 cut(s)	49, 177, 904
HgaI	GACGC	3 cut(s)	103, 1126, 1201
Hin1I	GRCGYC	2 cut(s)	95, 1212
Hin1II	CATG	6 cut(s)	131, 790, 1000, 1191, 1211, 1267
HindIII	AAGCTT	2 cut(s)	489, 636
HinfI	GANTC	4 cut(s)	77, 124, 682, 1024
HpaII	CCGG	3 cut(s)	49, 177, 904
HphI	GGTGA	6 cut(s)	38, 79, 709, 757, 893, 946
Hpy166II	GTNNAC	2 cut(s)	737, 1069
Hpy188I	TCNGA	2 cut(s)	210, 462
Hpy188III	TCNNGA	6 cut(s)	416, 515, 521, 626, 686, 820
Hpy8I	GTNNAC	2 cut(s)	737, 1069
Hpy99I	CGWCG	2 cut(s)	97, 157
HpyAV	CCTTC	3 cut(s)	64, 108, 154
HpyCH4III	ACNGT	2 cut(s)	453, 1033
HpyCH4IV	ACGT	3 cut(s)	259, 960, 1065
HpyF3I	CTNAG	3 cut(s)	459, 663, 678
HpySE526I	ACGT	3 cut(s)	259, 960, 1065
Hsp92I	GRCGYC	2 cut(s)	95, 1212
Hsp92II	CATG	6 cut(s)	131, 790, 1000, 1191, 1211, 1267
Kzo9I	GATC	6 cut(s)	137, 205, 403, 517, 816, 1101
LmnI	GCTCC	2 cut(s)	149, 182
Lsp1109I	GCAGC	5 cut(s)	514, 607, 1095, 1123, 1284
LweI	GCATC	2 cut(s)	48, 866
MaeI	CTAG	4 cut(s)	186, 276, 728, 1301
MaeII	ACGT	3 cut(s)	259, 960, 1065
MaeIII	GTNAC	7 cut(s)	44, 376, 934, 1220, 1237, 1267, 1277
MalI	GATC	6 cut(s)	139, 207, 405, 519, 818, 1103
MboI	GATC	6 cut(s)	137, 205, 403, 517, 816, 1101
MflI	RGATCY	2 cut(s)	403, 816
MhlI	GDGCHC	1 cut(s)	14
MlsI	TGGCCA	1 cut(s)	1163
MluCI	AATT	1 cut(s)	352
MluNI	TGGCCA	1 cut(s)	1163
MlyI	GAGTC	1 cut(s)	71
MmeI	TCCRAC	2 cut(s)	381, 963
Mox20I	TGGCCA	1 cut(s)	1163
MroXI	GAANNNNTTC	1 cut(s)	423
MscI	TGGCCA	1 cut(s)	1163
MseI	TTAA	1 cut(s)	803
Msp20I	TGGCCA	1 cut(s)	1163
MspA1I	CMGCKG	2 cut(s)	1108, 1114
MspI	CCGG	3 cut(s)	49, 177, 904
MspR9I	CCNGG	2 cut(s)	572, 1285
Mva1269I	GAATGC	1 cut(s)	1017
MvaI	CCWGG	2 cut(s)	572, 1285
NdeII	GATC	6 cut(s)	137, 205, 403, 517, 816, 1101
NheI	GCTAGC	1 cut(s)	727
NlaIII	CATG	6 cut(s)	131, 790, 1000, 1191, 1211, 1267
NlaIV	GGNNCC	4 cut(s)	12, 13, 405, 818
NmuCI	GTSAC	3 cut(s)	44, 934, 1267
PaeR7I	CTCGAG	1 cut(s)	758
PcsI	WCGNNNNNNNCGW	1 cut(s)	161
PctI	GAATGC	1 cut(s)	1017
PdmI	GAANNNNTTC	1 cut(s)	423
PfeI	GAWTC	3 cut(s)	124, 682, 1024
PfoI	TCCNGGA	1 cut(s)	570
PkrI	GCNGC	6 cut(s)	529, 622, 1110, 1113, 1147, 1299
PleI	GAGTC	1 cut(s)	71
PpsI	GAGTC	1 cut(s)	71
Ppu21I	YACGTR	1 cut(s)	1066
PpuMI	RGGWCCY	1 cut(s)	568
Psp5II	RGGWCCY	1 cut(s)	568
Psp6I	CCWGG	2 cut(s)	570, 1283
PspFI	CCCAGC	1 cut(s)	877
PspGI	CCWGG	2 cut(s)	570, 1283
PspN4I	GGNNCC	4 cut(s)	12, 13, 405, 818
PspOMI	GGGCCC	1 cut(s)	10
PspPI	GGNCC	4 cut(s)	10, 11, 296, 568
PspPPI	RGGWCCY	1 cut(s)	568
PspXI	VCTCGAGB	1 cut(s)	758
PstI	CTGCAG	3 cut(s)	385, 532, 1113
PstNI	CAGNNNCTG	2 cut(s)	527, 571
PsuI	RGATCY	2 cut(s)	403, 816
PvuII	CAGCTG	2 cut(s)	1108, 1114
RsaI	GTAC	6 cut(s)	247, 504, 696, 738, 853, 1043
RsaNI	GTAC	6 cut(s)	246, 503, 695, 737, 852, 1042
SaqAI	TTAA	1 cut(s)	803
SatI	GCNGC	6 cut(s)	528, 621, 1109, 1112, 1146, 1298
Sau3AI	GATC	6 cut(s)	137, 205, 403, 517, 816, 1101
Sau96I	GGNCC	4 cut(s)	10, 11, 296, 568
SchI	GAGTC	1 cut(s)	71
ScrFI	CCNGG	2 cut(s)	572, 1285
SduI	GDGCHC	1 cut(s)	14
SfaNI	GCATC	2 cut(s)	48, 866
SfcI	CTRYAG	3 cut(s)	381, 528, 1109
Sfr274I	CTCGAG	1 cut(s)	758
SinI	GGWCC	1 cut(s)	568
SlaI	CTCGAG	1 cut(s)	758
SmlI	CTYRAG	3 cut(s)	584, 626, 758
SmoI	CTYRAG	3 cut(s)	584, 626, 758
Sse9I	AATT	1 cut(s)	352
SsiI	CCGC	9 cut(s)	65, 81, 110, 135, 273, 721, 848, 1015, 1146
SspMI	CTAG	4 cut(s)	186, 276, 728, 1301
StyD4I	CCNGG	2 cut(s)	570, 1283
TaaI	ACNGT	2 cut(s)	453, 1033
TaiI	ACGT	3 cut(s)	262, 963, 1068
TaqI	TCGA	2 cut(s)	92, 759
TasI	AATT	1 cut(s)	352
TatI	WGTACW	4 cut(s)	245, 502, 736, 1041
TauI	GCSGC	1 cut(s)	1148
TfiI	GAWTC	3 cut(s)	124, 682, 1024
Tru1I	TTAA	1 cut(s)	803
Tru9I	TTAA	1 cut(s)	803
TscAI	CASTG	3 cut(s)	489, 682, 1038
TseFI	GTSAC	3 cut(s)	44, 934, 1267
TseI	GCWGC	5 cut(s)	527, 620, 1108, 1111, 1297
Tsp45I	GTSAC	3 cut(s)	44, 934, 1267
TspDTI	ATGAA	8 cut(s)	90, 116, 489, 669, 768, 985, 1193, 1252
TspGWI	ACGGA	2 cut(s)	32, 401
TspRI	CASTG	3 cut(s)	489, 682, 1038
VpaK11BI	GGWCC	1 cut(s)	568
XapI	RAATTY	1 cut(s)	352
XhoI	CTCGAG	1 cut(s)	758
XmnI	GAANNNNTTC	1 cut(s)	423
XspI	CTAG	4 cut(s)	186, 276, 728, 1301

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.