Rh1BG341400

magnesium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004630
Physical Location & Seq
Forward (+)
47502913 .. 47506567
3655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG341400.1

Sequence Viewer

Length: 1089 bp
ATGGGGAAAGGTCCGTTCTCGTTGAAGCGTAGCAGCAGCAGCAGCCGGCGGCAGCGGCCGAAGAAGTTTATCGGCCCTCCACCTTCTCCTTTCCCTTCGCCTCCTCCTCCTCCTCCTCCTCAGCCTCGGCCCATGGGATCCGCCCCCTCCTCGCCCTCGAACAACAACAGTTTGGCTGTCAAGGCCAAGAAGAAGGCCGGCGGAGGAGCCAGGCTCTGGATGCGATTCGATCGCTGCGGCGGTTCCGAGCTCGTCGAGTGTGACAAGAATGCTATTATCCGACGCGCCGCCATTCCCCCTCGTGACCTGAGAATTCTCGGCCCCGTTTTCTCCCATTCCTCCAGCATCCTCGCCAGGGAGAAGGCCATGGTGGTGAATTTGGAGTTTATCAAGGCTATAGTTACCGCCGAAGAGGTTCTGTTGCTTGATCCTCTTCGGCAGGAGGTTCTTCCATTTGCGGATCAACTAAGGCAACAGCTTCCTCAATCAAGGGTTGATGAACAAGATAATCAACTGGATGCTTCAACAGCAAGACAATGGTTACCTGTTCCCGAGGCCGTTGAAGGTTTGCAATGTGATCTCCCATTTGAGTTTCAGGTTCTCGAGATTGCTCTAGAAGTTGTCTGTACATACTTGGATTCCAGTGTGGCAGACCTTGAGATGGAAGCTTACCCTGTGCTCGATGAACTCGCTAGAAATGTCAGCACCAAGAATCTTGAACTTGTTCGCAGTTTGAAAAGCAATCTCACCCGTTTGCTTGCACGAGTACAGAAGGTGAGGGATGAAATTGAACATTTGTTGGATGACAATGAAGACATGGCACATTTATATTTGACAAGAAAGTGGATTCAGAATCAACAATCTGAGGCCTTGTTGGCACGTTCTGGCTCAAATAGAAGCAGCCCTACTGCACCCAATCTCCGTCGGCTAGGCTCTAATAGAAGCAGTAGTTTGGTAACTAGCAACAACATGGGTGAAGATGATGTAGAGGATCTGGAAATGTTGCTTGAGGCTTATTTTATGCAACTTGATGGAACTCGTAACAAGATATTGTCTGTAAGTGCAAATCCTCACTTTGCAACCTTATAA

Protein Analysis

362

Amino Acids

40.26

Weight (kDa)

6.93

Isoelectric Point (pI)

70.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRS2-like PF22099 86 - 286 9.5e-25 Magnesium transporter MRS2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1087
AccB7I CCANNNNNTGG 1 cut(s) 216
AccII CGCG 1 cut(s) 285
AciI CCGC 9 cut(s) 49, 55, 141, 201, 237, 240, 288, 405, 458
AclWI GGATC 5 cut(s) 132, 145, 422, 468, 999
AcoI YGGCCR 1 cut(s) 56
AcsI RAATTY 2 cut(s) 312, 376
AfaI GTAC 2 cut(s) 628, 768
AfiI CCNNNNNNNGG 3 cut(s) 216, 355, 661
AgsI TTSAA 6 cut(s) 25, 525, 563, 719, 736, 791
AjnI CCWGG 2 cut(s) 209, 353
AluBI AGCT 3 cut(s) 250, 478, 668
AluI AGCT 3 cut(s) 250, 478, 668
Alw21I GWGCWC 2 cut(s) 252, 681
AlwI GGATC 5 cut(s) 132, 145, 422, 468, 999
AlwNI CAGNNNCTG 1 cut(s) 216
Ama87I CYCGRG 2 cut(s) 551, 602
AoxI GGCC 9 cut(s) 56, 73, 128, 183, 195, 319, 363, 555, 867
ApeKI GCWGC 7 cut(s) 33, 36, 39, 42, 52, 234, 900
ApoI RAATTY 2 cut(s) 312, 376
Asp700I GAANNNNTTC 2 cut(s) 414, 723
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 4 cut(s) 11, 74, 129, 320
AsuHPI GGTGA 4 cut(s) 385, 739, 787, 986
AvaI CYCGRG 2 cut(s) 551, 602
AvaII GGWCC 1 cut(s) 11
BamHI GGATCC 1 cut(s) 137
BanII GRGCYC 1 cut(s) 252
BarI GAAGNNNNNNTAC 2 cut(s) 889, 921
BauI CACGAG 2 cut(s) 300, 762
BbsI GAAGAC 1 cut(s) 819
Bbv12I GWGCWC 2 cut(s) 252, 681
BbvCI CCTCAGC 1 cut(s) 120
BbvI GCAGC 7 cut(s) 45, 48, 51, 54, 64, 221, 912
BccI CCATC 2 cut(s) 655, 1025
BceAI ACGGC 1 cut(s) 542
BciT130I CCWGG 2 cut(s) 211, 355
BfaI CTAG 4 cut(s) 614, 693, 929, 960
BfmI CTRYAG 1 cut(s) 396
BglI GCCNNNNNGGC 1 cut(s) 875
Bme1390I CCNGG 2 cut(s) 211, 355
Bme18I GGWCC 1 cut(s) 11
BmeT110I CYCGRG 2 cut(s) 551, 602
BmgT120I GGNCC 4 cut(s) 11, 74, 129, 320
BmiI GGNNCC 4 cut(s) 139, 208, 244, 322
BmrFI CCNGG 2 cut(s) 211, 355
BmsI GCATC 3 cut(s) 210, 354, 508
BpiI GAAGAC 1 cut(s) 819
BplI GAGNNNNNCTC 2 cut(s) 198, 230
BpmI CTGGAG 1 cut(s) 325
Bpu10I CCTNAGC 1 cut(s) 120
BpuEI CTTGAG 2 cut(s) 677, 1028
BsaJI CCNNGG 5 cut(s) 125, 132, 354, 366, 552
BsaXI ACNNNNNCTCC 2 cut(s) 903, 933
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 355, 661
Bse118I RCCGGY 2 cut(s) 45, 197
Bse1I ACTGG 2 cut(s) 519, 642
Bse3DI GCAATG 1 cut(s) 578
BseBI CCWGG 2 cut(s) 211, 355
BseDI CCNNGG 5 cut(s) 125, 132, 354, 366, 552
BseGI GGATG 5 cut(s) 225, 345, 523, 787, 808
BseLI CCNNNNNNNGG 3 cut(s) 216, 355, 661
BseMI GCAATG 1 cut(s) 578
BseMII CTCAG 3 cut(s) 134, 299, 855
BseNI ACTGG 2 cut(s) 519, 642
BseRI GAGGAG 8 cut(s) 93, 96, 99, 102, 105, 108, 139, 219
BseX3I CGGCCG 1 cut(s) 56
BseXI GCAGC 7 cut(s) 45, 48, 51, 54, 64, 221, 912
BsgI GTGCAG 1 cut(s) 894
Bsh1236I CGCG 1 cut(s) 285
Bsh1285I CGRYCG 2 cut(s) 59, 232
BshFI GGCC 9 cut(s) 58, 75, 130, 185, 197, 321, 365, 557, 869
BsiEI CGRYCG 2 cut(s) 59, 232
BsiHKAI GWGCWC 2 cut(s) 252, 681
BsiHKCI CYCGRG 2 cut(s) 551, 602
BsiSI CCGG 2 cut(s) 46, 198
BslI CCNNNNNNNGG 3 cut(s) 216, 355, 661
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 9 cut(s) 58, 75, 130, 185, 197, 321, 365, 557, 869
BsoBI CYCGRG 2 cut(s) 551, 602
Bsp1286I GDGCHC 2 cut(s) 252, 681
Bsp1407I TGTACA 1 cut(s) 626
Bsp143I GATC 6 cut(s) 137, 229, 427, 460, 577, 991
Bsp19I CCATGG 2 cut(s) 132, 366
BspACI CCGC 9 cut(s) 49, 55, 141, 201, 237, 240, 288, 405, 458
BspANI GGCC 9 cut(s) 58, 75, 130, 185, 197, 321, 365, 557, 869
BspCNI CTCAG 3 cut(s) 133, 300, 856
BspFNI CGCG 1 cut(s) 285
BspLI GGNNCC 4 cut(s) 139, 208, 244, 322
BspPI GGATC 5 cut(s) 132, 145, 422, 468, 999
BsrDI GCAATG 1 cut(s) 578
BsrFI RCCGGY 2 cut(s) 45, 197
BsrGI TGTACA 1 cut(s) 626
BsrI ACTGG 2 cut(s) 519, 642
BssAI RCCGGY 2 cut(s) 45, 197
BssECI CCNNGG 5 cut(s) 125, 132, 354, 366, 552
BssMI GATC 6 cut(s) 137, 229, 427, 460, 577, 991
BssSI CACGAG 2 cut(s) 300, 762
BssT1I CCWWGG 2 cut(s) 132, 366
Bst2BI CACGAG 2 cut(s) 300, 762
Bst2UI CCWGG 2 cut(s) 211, 355
Bst4CI ACNGT 1 cut(s) 170
Bst6I CTCTTC 2 cut(s) 405, 438
BstAUI TGTACA 1 cut(s) 626
BstC8I GCNNGC 3 cut(s) 47, 199, 759
BstDEI CTNAG 4 cut(s) 120, 308, 467, 864
BstDSI CCRYGG 2 cut(s) 132, 366
BstEII GGTNACC 1 cut(s) 540
BstF5I GGATG 5 cut(s) 225, 345, 523, 787, 808
BstFNI CGCG 1 cut(s) 285
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 6 cut(s) 140, 232, 430, 463, 580, 994
BstMBI GATC 6 cut(s) 137, 229, 427, 460, 577, 991
BstMCI CGRYCG 2 cut(s) 59, 232
BstMWI GCNNNNNNNGC 7 cut(s) 39, 42, 55, 182, 220, 527, 875
BstNI CCWGG 2 cut(s) 211, 355
BstPI GGTNACC 1 cut(s) 540
BstSCI CCNGG 2 cut(s) 209, 353
BstSFI CTRYAG 1 cut(s) 396
BstUI CGCG 1 cut(s) 285
BstV1I GCAGC 7 cut(s) 45, 48, 51, 54, 64, 221, 912
BstV2I GAAGAC 1 cut(s) 819
BstX2I RGATCY 2 cut(s) 137, 991
BstYI RGATCY 2 cut(s) 137, 991
BstZI CGGCCG 1 cut(s) 56
BsuRI GGCC 9 cut(s) 58, 75, 130, 185, 197, 321, 365, 557, 869
BtgI CCRYGG 2 cut(s) 132, 366
BtsCI GGATG 5 cut(s) 225, 345, 523, 787, 808
BtsIMutI CAGTG 1 cut(s) 649
Cac8I GCNNGC 3 cut(s) 47, 199, 759
CaiI CAGNNNCTG 1 cut(s) 216
CfoI GCGC 1 cut(s) 287
Cfr10I RCCGGY 2 cut(s) 45, 197
Cfr13I GGNCC 4 cut(s) 11, 74, 129, 320
CseI GACGC 1 cut(s) 291
Csp6I GTAC 2 cut(s) 627, 767
CviAII CATG 4 cut(s) 133, 367, 817, 970
CviQI GTAC 2 cut(s) 627, 767
DdeI CTNAG 4 cut(s) 120, 308, 467, 864
DpnI GATC 6 cut(s) 139, 231, 429, 462, 579, 993
DpnII GATC 6 cut(s) 137, 229, 427, 460, 577, 991
EaeI YGGCCR 1 cut(s) 56
EagI CGGCCG 1 cut(s) 56
Eam1104I CTCTTC 2 cut(s) 405, 438
EarI CTCTTC 2 cut(s) 405, 438
EciI GGCGGA 2 cut(s) 130, 216
Ecl136II GAGCTC 1 cut(s) 250
EclXI CGGCCG 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 132, 366
Eco147I AGGCCT 1 cut(s) 869
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 11
Eco52I CGGCCG 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 250
Eco88I CYCGRG 2 cut(s) 551, 602
Eco91I GGTNACC 1 cut(s) 540
EcoICRI GAGCTC 1 cut(s) 250
EcoO65I GGTNACC 1 cut(s) 540
EcoRI GAATTC 1 cut(s) 312
EcoRII CCWGG 2 cut(s) 209, 353
EcoT14I CCWWGG 2 cut(s) 132, 366
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 2 cut(s) 132, 366
FaeI CATG 4 cut(s) 136, 370, 820, 973
FaiI YATR 9 cut(s) 134, 368, 398, 631, 818, 829, 971, 1022, 1087
FatI CATG 4 cut(s) 132, 366, 816, 969
FokI GGATG 5 cut(s) 232, 332, 530, 794, 815
FriOI GRGCYC 1 cut(s) 252
FspBI CTAG 4 cut(s) 614, 693, 929, 960
GlaI GCGC 1 cut(s) 286
GsuI CTGGAG 1 cut(s) 325
HaeIII GGCC 9 cut(s) 58, 75, 130, 185, 197, 321, 365, 557, 869
HapII CCGG 2 cut(s) 46, 198
HgaI GACGC 1 cut(s) 291
HhaI GCGC 1 cut(s) 287
Hin1II CATG 4 cut(s) 136, 370, 820, 973
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HindIII AAGCTT 1 cut(s) 666
HinfI GANTC 5 cut(s) 225, 638, 712, 847, 853
HpaII CCGG 2 cut(s) 46, 198
HphI GGTGA 4 cut(s) 385, 739, 787, 986
Hpy188I TCNGA 4 cut(s) 247, 281, 852, 865
Hpy188III TCNNGA 8 cut(s) 217, 302, 551, 602, 604, 614, 716, 995
Hpy99I CGWCG 3 cut(s) 257, 285, 927
HpyAV CCTTC 6 cut(s) 93, 105, 187, 355, 557, 766
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4IV ACGT 1 cut(s) 880
HpyCH4V TGCA 6 cut(s) 571, 761, 911, 1024, 1064, 1079
HpyF10VI GCNNNNNNNGC 7 cut(s) 39, 42, 55, 182, 220, 527, 875
HpyF3I CTNAG 4 cut(s) 120, 308, 467, 864
HpySE526I ACGT 1 cut(s) 880
Hsp92II CATG 4 cut(s) 136, 370, 820, 973
HspAI GCGC 1 cut(s) 285
KroI GCCGGC 2 cut(s) 45, 197
KroNI GCCGGC 2 cut(s) 47, 199
Kzo9I GATC 6 cut(s) 137, 229, 427, 460, 577, 991
LmnI GCTCC 1 cut(s) 206
Lsp1109I GCAGC 7 cut(s) 45, 48, 51, 54, 64, 221, 912
LweI GCATC 3 cut(s) 210, 354, 508
MaeI CTAG 4 cut(s) 614, 693, 929, 960
MaeII ACGT 1 cut(s) 880
MaeIII GTNAC 6 cut(s) 260, 302, 400, 540, 955, 1040
MalI GATC 6 cut(s) 139, 231, 429, 462, 579, 993
MboI GATC 6 cut(s) 137, 229, 427, 460, 577, 991
MboII GAAGA 7 cut(s) 73, 202, 422, 425, 440, 824, 989
MflI RGATCY 2 cut(s) 137, 991
MhlI GDGCHC 2 cut(s) 252, 681
MluCI AATT 3 cut(s) 312, 376, 786
MmeI TCCRAC 2 cut(s) 304, 780
MroNI GCCGGC 2 cut(s) 45, 197
MroXI GAANNNNTTC 2 cut(s) 414, 723
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 55
MspI CCGG 2 cut(s) 46, 198
MspR9I CCNGG 2 cut(s) 211, 355
Mva1269I GAATGC 1 cut(s) 274
MvaI CCWGG 2 cut(s) 211, 355
MvnI CGCG 1 cut(s) 285
MwoI GCNNNNNNNGC 7 cut(s) 39, 42, 55, 182, 220, 527, 875
NaeI GCCGGC 2 cut(s) 47, 199
NcoI CCATGG 2 cut(s) 132, 366
NdeII GATC 6 cut(s) 137, 229, 427, 460, 577, 991
NgoMIV GCCGGC 2 cut(s) 45, 197
NlaIII CATG 4 cut(s) 136, 370, 820, 973
NlaIV GGNNCC 4 cut(s) 139, 208, 244, 322
NmeAIII GCCGAG 2 cut(s) 106, 297
NmuCI GTSAC 2 cut(s) 260, 302
PaeR7I CTCGAG 1 cut(s) 602
PceI AGGCCT 1 cut(s) 869
PctI GAATGC 1 cut(s) 274
PdiI GCCGGC 2 cut(s) 47, 199
PdmI GAANNNNTTC 2 cut(s) 414, 723
PfeI GAWTC 5 cut(s) 225, 638, 712, 847, 853
PflMI CCANNNNNTGG 1 cut(s) 216
Ple19I CGATCG 1 cut(s) 232
PsiI TTATAA 1 cut(s) 1087
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 209, 353
PspEI GGTNACC 1 cut(s) 540
PspGI CCWGG 2 cut(s) 209, 353
PspN4I GGNNCC 4 cut(s) 139, 208, 244, 322
PspPI GGNCC 4 cut(s) 11, 74, 129, 320
PstNI CAGNNNCTG 1 cut(s) 216
PsuI RGATCY 2 cut(s) 137, 991
PvuI CGATCG 1 cut(s) 232
RsaI GTAC 2 cut(s) 628, 768
RsaNI GTAC 2 cut(s) 627, 767
RseI CAYNNNNRTG 1 cut(s) 371
SacI GAGCTC 1 cut(s) 252
Sau3AI GATC 6 cut(s) 137, 229, 427, 460, 577, 991
Sau96I GGNCC 4 cut(s) 11, 74, 129, 320
ScrFI CCNGG 2 cut(s) 211, 355
SduI GDGCHC 2 cut(s) 252, 681
SfaNI GCATC 3 cut(s) 210, 354, 508
SfcI CTRYAG 1 cut(s) 396
Sfr274I CTCGAG 1 cut(s) 602
SinI GGWCC 1 cut(s) 11
SlaI CTCGAG 1 cut(s) 602
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 3 cut(s) 602, 656, 1007
SmoI CTYRAG 3 cut(s) 602, 656, 1007
Sse9I AATT 3 cut(s) 312, 376, 786
SseBI AGGCCT 1 cut(s) 869
SsiI CCGC 9 cut(s) 49, 55, 141, 201, 237, 240, 288, 405, 458
SspMI CTAG 4 cut(s) 614, 693, 929, 960
SstI GAGCTC 1 cut(s) 252
StuI AGGCCT 1 cut(s) 869
StyD4I CCNGG 2 cut(s) 209, 353
StyI CCWWGG 2 cut(s) 132, 366
TaaI ACNGT 1 cut(s) 170
TaiI ACGT 1 cut(s) 883
TaqI TCGA 5 cut(s) 158, 228, 255, 603, 681
TasI AATT 3 cut(s) 312, 376, 786
TatI WGTACW 2 cut(s) 626, 766
TauI GCSGC 4 cut(s) 52, 58, 240, 290
TfiI GAWTC 5 cut(s) 225, 638, 712, 847, 853
TscAI CASTG 1 cut(s) 649
TseFI GTSAC 2 cut(s) 260, 302
TseI GCWGC 7 cut(s) 33, 36, 39, 42, 52, 234, 900
Tsp45I GTSAC 2 cut(s) 260, 302
TspDTI ATGAA 4 cut(s) 513, 699, 798, 825
TspGWI ACGGA 1 cut(s) 911
TspRI CASTG 1 cut(s) 649
Van91I CCANNNNNTGG 1 cut(s) 216
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 2 cut(s) 312, 376
XbaI TCTAGA 1 cut(s) 613
XhoI CTCGAG 1 cut(s) 602
XmnI GAANNNNTTC 2 cut(s) 414, 723
XspI CTAG 4 cut(s) 614, 693, 929, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.