Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004630

Physical Location & Seq

Genomic Coordinates Forward (+)

30550931 .. 30555002

Gene Structure 4072 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1238500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1383 bp

ATGGGGAAGGGTCCCTTCTCTTTCAAGCGCAGCAGCAGCAGCGTACGACGCCGCCCCAAGAAGCTCATTGGCACTCCACCTTCCCCTTTTCCCTCTCCGCCGCAGCCGATTCGGTCTCCCCCGCCCTCCAACAGTGTCAATGTTAACAGTACCAATACGAATAATACTAGTCTCAGCGCGGTTGTTGCTGGCGGCGCCGGCGGTGGAGTGAAGGGGAAGAAGAAGGCTGGAGGAGCCAGGCTTTGGATGCGGTTTGACCGCTTCGGCGTATCAGAGCTCGTTGAGTGTGATAAGAACGCTATCATTCGACGCGCTGCCATTCCCGCCCGGGACTTGAGGATTCTCGGCCCTGTCTTCTCCCAATCCTCCAGCATCCTTGCCAGGGAGAAGGCCATGGTGGTTAATTTAGAGTTTATAAAGGCTATAGTTACAGCCGAAGAGGTTTTGTTGCTTGATCCTCTTCGGCAGGAGGTTCTTCCATTTGCAGATCAGCTAAGGCAACAACTTCCTCAGAAAAGCCAATCCAGGATACAAGAAGCAAGTCCACTTGATGAACAAGATAATGGAATGGATGCTTCAACTATAAGGCAATGGTTACCTGTTCCCGAGGCTGTTGAAGGATTGCAGTGTGATCTCCCATTTGAGTTTCAGGTTCTGGAGGTTGCATTAGAAGTTGTCTGTATATACTTGGATTCTAGTGTGGCAGAACTTGAGAGAGAAGCCTACCCTGTGTTAGATGAACTGGCTAGAAATGTTAGCACCAAAAACCTTGAACTTGTGAGGAGTTTGAAAAGCAATCTTACCCGTTTGCTTGCACGTGTCCAGAAGGTGAGGGATGAAATTGAACATTTGTTGGATGACAATGAAGATATGGCACAATTATATTTAACAAGGAAATGGATGCAGAATCAACAGCCTGAGGCTTTGTTGGGGCGTTCTGCATCAAATAGAATCTCTGCACCCCATCTTCGTCGGCTTGGTTCTAACAGAAGTGGGAGTTTGGTAACCAGTAACAATATGGACTACGATGATGTAGAAGATCTAGAGATGCTGCTTGAGGCATATTTTATGCAGCTGGATGGAACCCGTAACAAGATATTATCTGTGCGGGAGTATATTGATGACACGGAGGATTATGTCAACATACAACTTGACAACCAACGAAACGAACTCATTCAGCTCCAGTTGACATTGACCATTGCATCATTTGCGGTAGCGGTGGAGACCTTGATATCTGGGATATTTGGTATGAACATCCCCTGTATCTTATACGACAAGGATGGGATATTTGAGGCCTTTGTTGGGGGTATTACAGCATTTTCCGTGTTACTCTTCTTTCTCGTCTTCGGGTATGCGAGGTGGAAAAAGCTGCTTGGTACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0007275 GO:0008150 GO:0008324 GO:0009555 GO:0015075 GO:0015095 GO:0015318 GO:0015693 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0032501 GO:0032502 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0055085 GO:0070838 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1903830

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CorA

Protein Analysis

461

Amino Acids

51.34

Weight (kDa)

5.62

Isoelectric Point (pI)

58.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MRS2-like	PF22099	95 - 304	3.6e-21	Magnesium transporter MRS2-like
MRS2-like	PF22099	336 - 388	9.3e-07	Magnesium transporter MRS2-like
CorA	PF01544	352 - 449	6.8e-07	CorA-like Mg2+ transporter protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004630)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58970
fragaria_vesca	FvH4_5g28740 FvH4_5g28740 FvH4_5g28740 FvH4_5g28740 FvH4_5g28740 FvH4_5g28740
malus_domestica	MD08G1238500.v1.1 MD08G1238700.v1.1 MD15G1430500.v1.1
prunus_persica	Prupe.1G575700_v2.0.a1
pyrus_communis	pycom08g20700 pycom08g20720 pycom15g38020 pycom420g00390 pycom420g00500 pycom420g00530
rosa_chinensis	RchiOBHm_Chr1g0372101 RchiOBHm_Chr6g0246761
rosa_laevigata	RLG00000015250 RLG00000026923
rosa_multiflora	Rmu_sc0001755.1_g000027 Rmu_sc0003131.1_g000033
rosa_roxburghii	Rroxscaffold_4G00285260 Rroxscaffold_7G00214470
rosa_rugosa	Rorug01G0369700
rosa_samantha	Rh1AG378000 Rh1BG341400 Rh1CG354800 Rh1DG372500
rosa_wichuraiana	Rw1G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	416
AccB1I	GGYRCC	1 cut(s)	194
AccB7I	CCANNNNNTGG	1 cut(s)	243
AccII	CGCG	2 cut(s)	179, 312
AclWI	GGATC	1 cut(s)	449
AcvI	CACGTG	1 cut(s)	818
AcyI	GRCGYC	2 cut(s)	49, 195
AfaI	GTAC	3 cut(s)	45, 151, 1378
AfiI	CCNNNNNNNGG	3 cut(s)	243, 382, 1302
AflIII	ACRYGT	1 cut(s)	817
AgsI	TTSAA	6 cut(s)	25, 579, 617, 773, 790, 845
AhlI	ACTAGT	1 cut(s)	167
AjnI	CCWGG	3 cut(s)	236, 380, 524
AluBI	AGCT	6 cut(s)	64, 277, 493, 1075, 1180, 1369
AluI	AGCT	6 cut(s)	64, 277, 493, 1075, 1180, 1369
Alw21I	GWGCWC	1 cut(s)	279
Alw26I	GTCTC	3 cut(s)	120, 176, 1217
AlwI	GGATC	1 cut(s)	449
AlwNI	CAGNNNCTG	1 cut(s)	655
Ama87I	CYCGRG	2 cut(s)	327, 605
AoxI	GGCC	3 cut(s)	346, 390, 1293
ApeKI	GCWGC	9 cut(s)	30, 33, 36, 39, 103, 314, 1051, 1072, 1369
Asp700I	GAANNNNTTC	1 cut(s)	1173
AspLEI	GCGC	4 cut(s)	30, 179, 197, 314
AspS9I	GGNCC	2 cut(s)	11, 347
AsuC2I	CCSGG	2 cut(s)	328, 329
AsuHPI	GGTGA	1 cut(s)	841
AvaI	CYCGRG	2 cut(s)	327, 605
AvaII	GGWCC	1 cut(s)	11
AxyI	CCTNAGG	1 cut(s)	918
BanI	GGYRCC	1 cut(s)	194
BanII	GRGCYC	1 cut(s)	279
BbrPI	CACGTG	1 cut(s)	818
BbsI	GAAGAC	2 cut(s)	346, 1336
Bbv12I	GWGCWC	1 cut(s)	279
BbvI	GCAGC	9 cut(s)	42, 45, 48, 51, 115, 301, 1038, 1084, 1356
BccI	CCATC	3 cut(s)	972, 1073, 1274
BcgI	CGANNNNNNTGC	2 cut(s)	92, 126
BciT130I	CCWGG	3 cut(s)	238, 382, 526
BciVI	GTATCC	1 cut(s)	522
BcnI	CCSGG	2 cut(s)	328, 329
BcoDI	GTCTC	3 cut(s)	120, 176, 1217
BcuI	ACTAGT	1 cut(s)	167
BfaI	CTAG	4 cut(s)	168, 696, 747, 1043
BfmI	CTRYAG	1 cut(s)	423
BfoI	RGCGCY	1 cut(s)	198
BfuI	GTATCC	1 cut(s)	522
BglII	AGATCT	1 cut(s)	1039
Bme1390I	CCNGG	5 cut(s)	238, 328, 329, 382, 526
Bme18I	GGWCC	1 cut(s)	11
BmeT110I	CYCGRG	2 cut(s)	327, 605
BmgT120I	GGNCC	2 cut(s)	11, 347
BmiI	GGNNCC	5 cut(s)	12, 13, 196, 235, 1084
BmrFI	CCNGG	5 cut(s)	238, 328, 329, 382, 526
BmsI	GCATC	7 cut(s)	237, 381, 562, 891, 950, 1038, 1211
BpiI	GAAGAC	2 cut(s)	346, 1336
BpmI	CTGGAG	4 cut(s)	249, 352, 677, 1166
Bpu10I	CCTNAGC	1 cut(s)	494
BpuEI	CTTGAG	3 cut(s)	355, 731, 1076
BpuMI	CCSGG	2 cut(s)	328, 329
BsaAI	YACGTR	1 cut(s)	818
BsaHI	GRCGYC	2 cut(s)	49, 195
BsaI	GGTCTC	2 cut(s)	120, 1217
BsaJI	CCNNGG	4 cut(s)	327, 381, 393, 606
Bsc4I	CCNNNNNNNGG	3 cut(s)	243, 382, 1302
Bse118I	RCCGGY	1 cut(s)	197
Bse1I	ACTGG	3 cut(s)	747, 1008, 1183
Bse21I	CCTNAGG	1 cut(s)	918
Bse3DI	GCAATG	2 cut(s)	596, 1197
BseBI	CCWGG	3 cut(s)	238, 382, 526
BseDI	CCNNGG	4 cut(s)	327, 381, 393, 606
BseGI	GGATG	9 cut(s)	252, 372, 577, 841, 862, 906, 1084, 1254, 1285
BseLI	CCNNNNNNNGG	3 cut(s)	243, 382, 1302
BseMI	GCAATG	2 cut(s)	596, 1197
BseMII	CTCAG	3 cut(s)	187, 524, 909
BseNI	ACTGG	3 cut(s)	747, 1008, 1183
BseRI	GAGGAG	2 cut(s)	246, 796
BseXI	GCAGC	9 cut(s)	42, 45, 48, 51, 115, 301, 1038, 1084, 1356
BsgI	GTGCAG	1 cut(s)	942
Bsh1236I	CGCG	2 cut(s)	179, 312
BshFI	GGCC	3 cut(s)	348, 392, 1295
BshNI	GGYRCC	1 cut(s)	194
BsiHKAI	GWGCWC	1 cut(s)	279
BsiHKCI	CYCGRG	2 cut(s)	327, 605
BsiSI	CCGG	2 cut(s)	198, 328
BsiWI	CGTACG	1 cut(s)	43
BslFI	GGGAC	1 cut(s)	344
BslI	CCNNNNNNNGG	3 cut(s)	243, 382, 1302
BsmAI	GTCTC	3 cut(s)	120, 176, 1217
BsmFI	GGGAC	1 cut(s)	344
BsnI	GGCC	3 cut(s)	348, 392, 1295
Bso31I	GGTCTC	2 cut(s)	120, 1217
BsoBI	CYCGRG	2 cut(s)	327, 605
Bsp1286I	GDGCHC	1 cut(s)	279
Bsp143I	GATC	4 cut(s)	454, 487, 631, 1039
Bsp19I	CCATGG	1 cut(s)	393
BspANI	GGCC	3 cut(s)	348, 392, 1295
BspCNI	CTCAG	3 cut(s)	186, 523, 910
BspFNI	CGCG	2 cut(s)	179, 312
BspLI	GGNNCC	5 cut(s)	12, 13, 196, 235, 1084
BspPI	GGATC	1 cut(s)	449
BspT107I	GGYRCC	1 cut(s)	194
BspTNI	GGTCTC	2 cut(s)	120, 1217
BsrDI	GCAATG	2 cut(s)	596, 1197
BsrFI	RCCGGY	1 cut(s)	197
BsrI	ACTGG	3 cut(s)	747, 1008, 1183
BssAI	RCCGGY	1 cut(s)	197
BssECI	CCNNGG	4 cut(s)	327, 381, 393, 606
BssMI	GATC	4 cut(s)	454, 487, 631, 1039
BssNI	GRCGYC	2 cut(s)	49, 195
BssT1I	CCWWGG	1 cut(s)	393
Bst2UI	CCWGG	3 cut(s)	238, 382, 526
Bst4CI	ACNGT	2 cut(s)	134, 149
Bst6I	CTCTTC	3 cut(s)	432, 465, 1337
BstACI	GRCGYC	2 cut(s)	49, 195
BstAPI	GCANNNNNTGC	1 cut(s)	1208
BstBAI	YACGTR	1 cut(s)	818
BstC8I	GCNNGC	3 cut(s)	190, 199, 813
BstDEI	CTNAG	4 cut(s)	173, 494, 510, 918
BstDSI	CCRYGG	1 cut(s)	393
BstEII	GGTNACC	2 cut(s)	594, 1003
BstF5I	GGATG	9 cut(s)	252, 372, 577, 841, 862, 906, 1084, 1254, 1285
BstFNI	CGCG	2 cut(s)	179, 312
BstH2I	RGCGCY	1 cut(s)	198
BstHHI	GCGC	4 cut(s)	30, 179, 197, 314
BstKTI	GATC	4 cut(s)	457, 490, 634, 1042
BstMAI	GTCTC	3 cut(s)	120, 176, 1217
BstMBI	GATC	4 cut(s)	454, 487, 631, 1039
BstNI	CCWGG	3 cut(s)	238, 382, 526
BstPI	GGTNACC	2 cut(s)	594, 1003
BstSCI	CCNGG	5 cut(s)	236, 326, 327, 380, 524
BstSFI	CTRYAG	1 cut(s)	423
BstUI	CGCG	2 cut(s)	179, 312
BstV1I	GCAGC	9 cut(s)	42, 45, 48, 51, 115, 301, 1038, 1084, 1356
BstV2I	GAAGAC	2 cut(s)	346, 1336
BstX2I	RGATCY	1 cut(s)	1039
BstYI	RGATCY	1 cut(s)	1039
Bsu36I	CCTNAGG	1 cut(s)	918
BsuI	GTATCC	1 cut(s)	522
BsuRI	GGCC	3 cut(s)	348, 392, 1295
BtgI	CCRYGG	1 cut(s)	393
BtsCI	GGATG	9 cut(s)	252, 372, 577, 841, 862, 906, 1084, 1254, 1285
BtsI	GCAGTG	1 cut(s)	632
BtsIMutI	CAGTG	2 cut(s)	139, 632
Cac8I	GCNNGC	3 cut(s)	190, 199, 813
CaiI	CAGNNNCTG	1 cut(s)	655
CfoI	GCGC	4 cut(s)	30, 179, 197, 314
Cfr10I	RCCGGY	1 cut(s)	197
Cfr13I	GGNCC	2 cut(s)	11, 347
Cfr9I	CCCGGG	1 cut(s)	327
CseI	GACGC	2 cut(s)	57, 318
Csp6I	GTAC	3 cut(s)	44, 150, 1377
CviAII	CATG	2 cut(s)	394, 1380
CviQI	GTAC	3 cut(s)	44, 150, 1377
DdeI	CTNAG	4 cut(s)	173, 494, 510, 918
DinI	GGCGCC	1 cut(s)	196
DpnI	GATC	4 cut(s)	456, 489, 633, 1041
DpnII	GATC	4 cut(s)	454, 487, 631, 1039
Eam1104I	CTCTTC	3 cut(s)	432, 465, 1337
EarI	CTCTTC	3 cut(s)	432, 465, 1337
EciI	GGCGGA	1 cut(s)	87
Ecl136II	GAGCTC	1 cut(s)	277
Eco130I	CCWWGG	1 cut(s)	393
Eco147I	AGGCCT	1 cut(s)	1295
Eco24I	GRGCYC	1 cut(s)	279
Eco31I	GGTCTC	2 cut(s)	120, 1217
Eco32I	GATATC	1 cut(s)	1233
Eco47I	GGWCC	1 cut(s)	11
Eco53kI	GAGCTC	1 cut(s)	277
Eco72I	CACGTG	1 cut(s)	818
Eco81I	CCTNAGG	1 cut(s)	918
Eco88I	CYCGRG	2 cut(s)	327, 605
Eco91I	GGTNACC	2 cut(s)	594, 1003
EcoICRI	GAGCTC	1 cut(s)	277
EcoO109I	RGGNCCY	1 cut(s)	11
EcoO65I	GGTNACC	2 cut(s)	594, 1003
EcoRII	CCWGG	3 cut(s)	236, 380, 524
EcoRV	GATATC	1 cut(s)	1233
EcoT14I	CCWWGG	1 cut(s)	393
EcoT38I	GRGCYC	1 cut(s)	279
EgeI	GGCGCC	1 cut(s)	196
EheI	GGCGCC	1 cut(s)	196
ErhI	CCWWGG	1 cut(s)	393
FaeI	CATG	2 cut(s)	397, 1383
FalI	AAGNNNNNCTT	1 cut(s)	31
FaqI	GGGAC	1 cut(s)	344
FatI	CATG	2 cut(s)	393, 1379
FauI	CCCGC	3 cut(s)	129, 331, 1101
FokI	GGATG	9 cut(s)	259, 359, 584, 848, 869, 913, 1091, 1241, 1292
FriOI	GRGCYC	1 cut(s)	279
FspBI	CTAG	4 cut(s)	168, 696, 747, 1043
GlaI	GCGC	4 cut(s)	29, 178, 196, 313
GsuI	CTGGAG	4 cut(s)	249, 352, 677, 1166
HaeII	RGCGCY	1 cut(s)	198
HaeIII	GGCC	3 cut(s)	348, 392, 1295
HapII	CCGG	2 cut(s)	198, 328
HgaI	GACGC	2 cut(s)	57, 318
HhaI	GCGC	4 cut(s)	30, 179, 197, 314
Hin1I	GRCGYC	2 cut(s)	49, 195
Hin1II	CATG	2 cut(s)	397, 1383
Hin6I	GCGC	4 cut(s)	28, 177, 195, 312
HinP1I	GCGC	4 cut(s)	28, 177, 195, 312
HincII	GTYRAC	3 cut(s)	145, 1141, 1188
HindII	GTYRAC	3 cut(s)	145, 1141, 1188
HinfI	GANTC	5 cut(s)	109, 340, 692, 907, 951
HpaI	GTTAAC	1 cut(s)	145
HpaII	CCGG	2 cut(s)	198, 328
HphI	GGTGA	1 cut(s)	841
Hpy166II	GTNNAC	4 cut(s)	145, 545, 1141, 1188
Hpy188I	TCNGA	2 cut(s)	274, 513
Hpy188III	TCNNGA	4 cut(s)	605, 656, 823, 1043
Hpy8I	GTNNAC	4 cut(s)	145, 545, 1141, 1188
Hpy99I	CGWCG	3 cut(s)	51, 312, 975
HpyAV	CCTTC	7 cut(s)	25, 90, 205, 217, 382, 611, 820
HpyCH4III	ACNGT	2 cut(s)	134, 149
HpyCH4IV	ACGT	1 cut(s)	817
HpyCH4V	TGCA	9 cut(s)	485, 625, 665, 815, 904, 941, 959, 1072, 1202
HpyF3I	CTNAG	4 cut(s)	173, 494, 510, 918
HpySE526I	ACGT	1 cut(s)	817
Hsp92I	GRCGYC	2 cut(s)	49, 195
Hsp92II	CATG	2 cut(s)	397, 1383
HspAI	GCGC	4 cut(s)	28, 177, 195, 312
KasI	GGCGCC	1 cut(s)	194
KflI	GGGWCCC	1 cut(s)	11
KroI	GCCGGC	1 cut(s)	197
KroNI	GCCGGC	1 cut(s)	199
KspAI	GTTAAC	1 cut(s)	145
Kzo9I	GATC	4 cut(s)	454, 487, 631, 1039
LmnI	GCTCC	2 cut(s)	233, 1185
Lsp1109I	GCAGC	9 cut(s)	42, 45, 48, 51, 115, 301, 1038, 1084, 1356
LweI	GCATC	7 cut(s)	237, 381, 562, 891, 950, 1038, 1211
MaeI	CTAG	4 cut(s)	168, 696, 747, 1043
MaeII	ACGT	1 cut(s)	817
MaeIII	GTNAC	6 cut(s)	427, 594, 1003, 1010, 1088, 1326
MalI	GATC	4 cut(s)	456, 489, 633, 1041
MboI	GATC	4 cut(s)	454, 487, 631, 1039
MflI	RGATCY	1 cut(s)	1039
MhlI	GDGCHC	1 cut(s)	279
MluCI	AATT	3 cut(s)	403, 840, 878
Mly113I	GGCGCC	1 cut(s)	195
MmeI	TCCRAC	2 cut(s)	153, 834
MreI	CGCCGGCG	1 cut(s)	197
MroNI	GCCGGC	1 cut(s)	197
MroXI	GAANNNNTTC	1 cut(s)	1173
MseI	TTAA	3 cut(s)	144, 402, 887
MspA1I	CMGCKG	1 cut(s)	1075
MspI	CCGG	2 cut(s)	198, 328
MspR9I	CCNGG	5 cut(s)	238, 328, 329, 382, 526
MvaI	CCWGG	3 cut(s)	238, 382, 526
MvnI	CGCG	2 cut(s)	179, 312
NaeI	GCCGGC	1 cut(s)	199
NarI	GGCGCC	1 cut(s)	195
NciI	CCSGG	2 cut(s)	328, 329
NcoI	CCATGG	1 cut(s)	393
NdeII	GATC	4 cut(s)	454, 487, 631, 1039
NgoMIV	GCCGGC	1 cut(s)	197
NlaIII	CATG	2 cut(s)	397, 1383
NlaIV	GGNNCC	5 cut(s)	12, 13, 196, 235, 1084
NmeAIII	GCCGAG	1 cut(s)	324
PceI	AGGCCT	1 cut(s)	1295
PdiI	GCCGGC	1 cut(s)	199
PdmI	GAANNNNTTC	1 cut(s)	1173
PfeI	GAWTC	5 cut(s)	109, 340, 692, 907, 951
Pfl23II	CGTACG	1 cut(s)	43
PflMI	CCANNNNNTGG	1 cut(s)	243
PfoI	TCCNGGA	1 cut(s)	524
PluTI	GGCGCC	1 cut(s)	198
PmaCI	CACGTG	1 cut(s)	818
PmlI	CACGTG	1 cut(s)	818
Ppu21I	YACGTR	1 cut(s)	818
PpuMI	RGGWCCY	1 cut(s)	11
PsiI	TTATAA	1 cut(s)	416
Psp124BI	GAGCTC	1 cut(s)	279
Psp5II	RGGWCCY	1 cut(s)	11
Psp6I	CCWGG	3 cut(s)	236, 380, 524
PspCI	CACGTG	1 cut(s)	818
PspEI	GGTNACC	2 cut(s)	594, 1003
PspGI	CCWGG	3 cut(s)	236, 380, 524
PspLI	CGTACG	1 cut(s)	43
PspN4I	GGNNCC	5 cut(s)	12, 13, 196, 235, 1084
PspPI	GGNCC	2 cut(s)	11, 347
PspPPI	RGGWCCY	1 cut(s)	11
PstNI	CAGNNNCTG	1 cut(s)	655
PsuI	RGATCY	1 cut(s)	1039
PvuII	CAGCTG	1 cut(s)	1075
RsaI	GTAC	3 cut(s)	45, 151, 1378
RsaNI	GTAC	3 cut(s)	44, 150, 1377
SacI	GAGCTC	1 cut(s)	279
SaqAI	TTAA	3 cut(s)	144, 402, 887
Sau3AI	GATC	4 cut(s)	454, 487, 631, 1039
Sau96I	GGNCC	2 cut(s)	11, 347
ScrFI	CCNGG	5 cut(s)	238, 328, 329, 382, 526
SduI	GDGCHC	1 cut(s)	279
SfaNI	GCATC	7 cut(s)	237, 381, 562, 891, 950, 1038, 1211
SfcI	CTRYAG	1 cut(s)	423
SfoI	GGCGCC	1 cut(s)	196
SgrAI	CRCCGGYG	1 cut(s)	197
SinI	GGWCC	1 cut(s)	11
SmaI	CCCGGG	1 cut(s)	329
SmlI	CTYRAG	3 cut(s)	334, 710, 1055
SmoI	CTYRAG	3 cut(s)	334, 710, 1055
SpeI	ACTAGT	1 cut(s)	167
Sse9I	AATT	3 cut(s)	403, 840, 878
SseBI	AGGCCT	1 cut(s)	1295
SspDI	GGCGCC	1 cut(s)	194
SspMI	CTAG	4 cut(s)	168, 696, 747, 1043
SstI	GAGCTC	1 cut(s)	279
StuI	AGGCCT	1 cut(s)	1295
StyD4I	CCNGG	5 cut(s)	236, 326, 327, 380, 524
StyI	CCWWGG	1 cut(s)	393
TaaI	ACNGT	2 cut(s)	134, 149
TaiI	ACGT	1 cut(s)	820
TaqI	TCGA	1 cut(s)	307
TaqII	GACCGA	1 cut(s)	102
TasI	AATT	3 cut(s)	403, 840, 878
TauI	GCSGC	3 cut(s)	54, 103, 195
TfiI	GAWTC	5 cut(s)	109, 340, 692, 907, 951
Tru1I	TTAA	3 cut(s)	144, 402, 887
Tru9I	TTAA	3 cut(s)	144, 402, 887
TscAI	CASTG	2 cut(s)	139, 632
TseI	GCWGC	9 cut(s)	30, 33, 36, 39, 103, 314, 1051, 1072, 1369
TspDTI	ATGAA	5 cut(s)	567, 753, 852, 879, 1265
TspGWI	ACGGA	2 cut(s)	1142, 1312
TspMI	CCCGGG	1 cut(s)	327
TspRI	CASTG	2 cut(s)	139, 632
Van91I	CCANNNNNTGG	1 cut(s)	243
VpaK11BI	GGWCC	1 cut(s)	11
XbaI	TCTAGA	1 cut(s)	1042
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1015
XmaI	CCCGGG	1 cut(s)	327
XmnI	GAANNNNTTC	1 cut(s)	1173
XspI	CTAG	4 cut(s)	168, 696, 747, 1043

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.