AT3G61560

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002430
Physical Location & Seq
Forward (+)
22777741 .. 22780191
2451 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G61560.2

Sequence Viewer

Length: 762 bp
ATGGCGGAAGAATTGGAGAAATCTATGCACAAGGAAGAATCTCTGATGGAGAAGATCGCTGATAAGATCCACGATCACGACTCTTCGTCGTCGTCTGATTCCGAGCACGAGAAACCAGAGTCTCCGTCAGCTTTGAAGGCCAAGATATATCGTTTGTTTGGTAGAGAGAAGCCTGTTCATAAGGTTCTCGGTGGTGGCTTACCGGCTGATGTGTTCTTGTGGAGGGACAAAAAACTCTCAGCTTCTGTTCTTGGTGTAGCGACTGCGATATGGGTTCTGTTTGAATTGGTTGAGTATCATTTCTTGAGTCTTGTGTGTCACATTTTGATATTCGCTCTCGCAGCCCTGTTTTTATTGTCCAATGCTCATGCCTTTATGAACAAGACTCCACCTAAAATTCCTGAAATCCATATTAAAGAGGAACATTTCCTTATGATTGTTTCTGCACTGAGAAACGAGCTGAACCAGGCCTTTGTTATTTTAAGGAGCATTGCCTTAGGAAGGGACTTGAAGAAATTTCTCATGGTGGTTTTTGGGCTGTGGATAATCTCGGTTGTGGGAAACTGGTTCAACTTCTTGACTCTTGTATACATCTGTTTTGTGGTGTTACATACAGTACCAATGTTGTATGAGAAACATGAGGACAAGGTAGATCCAGTGGCAGAGAAGACGTTGAAGGAGCTGAAGAAACATTACATGGTGTTTGATGAGAAGGTTCTTTCTAAGCTTCCTGTTGCTTCCCTTAAGGCAAAGTTGGGTTAA

Protein Analysis

253

Amino Acids

28.82

Weight (kDa)

7.82

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 69 - 223 1.4e-57 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 588
AciI CCGC 1 cut(s) 5
AclWI GGATC 2 cut(s) 61, 647
AcsI RAATTY 2 cut(s) 396, 515
AcuI CTGAAG 1 cut(s) 704
AfaI GTAC 1 cut(s) 618
AfiI CCNNNNNNNGG 1 cut(s) 501
AflII CTTAAG 1 cut(s) 743
AgsI TTSAA 5 cut(s) 136, 284, 511, 571, 676
AhdI GACNNNNNGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 465
AluBI AGCT 5 cut(s) 131, 242, 460, 682, 727
AluI AGCT 5 cut(s) 131, 242, 460, 682, 727
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 2 cut(s) 61, 647
AlwNI CAGNNNCTG 1 cut(s) 245
AoxI GGCC 2 cut(s) 138, 468
ApeKI GCWGC 1 cut(s) 341
ApoI RAATTY 2 cut(s) 396, 515
AxyI CCTNAGG 1 cut(s) 496
BarI GAAGNNNNNNTAC 2 cut(s) 677, 709
BauI CACGAG 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 674
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 1 cut(s) 353
BccI CCATC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 467
BcoDI GTCTC 1 cut(s) 126
BfrI CTTAAG 1 cut(s) 743
BisI GCNGC 1 cut(s) 342
BlsI GCNGC 1 cut(s) 343
Bme1390I CCNGG 1 cut(s) 467
BmeRI GACNNNNNGTC 1 cut(s) 85
BmrFI CCNGG 1 cut(s) 467
BpiI GAAGAC 1 cut(s) 674
BpuEI CTTGAG 1 cut(s) 325
Bsc4I CCNNNNNNNGG 1 cut(s) 501
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 569, 656
Bse21I CCTNAGG 1 cut(s) 496
Bse3DI GCAATG 1 cut(s) 489
BseBI CCWGG 1 cut(s) 467
BseLI CCNNNNNNNGG 1 cut(s) 501
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 2 cut(s) 252, 440
BseNI ACTGG 2 cut(s) 569, 656
BseXI GCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 429
BshFI GGCC 2 cut(s) 140, 470
BsiHKAI GWGCWC 1 cut(s) 108
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 2 cut(s) 239, 518
BslI CCNNNNNNNGG 1 cut(s) 501
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 2 cut(s) 239, 518
BsnI GGCC 2 cut(s) 140, 470
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 4 cut(s) 54, 66, 73, 652
BspACI CCGC 1 cut(s) 5
BspANI GGCC 2 cut(s) 140, 470
BspCNI CTCAG 2 cut(s) 251, 441
BspPI GGATC 2 cut(s) 61, 647
BspTI CTTAAG 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 489
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 2 cut(s) 569, 656
BssAI RCCGGY 1 cut(s) 202
BssMI GATC 4 cut(s) 54, 66, 73, 652
BssNAI GTATAC 1 cut(s) 589
BssSI CACGAG 1 cut(s) 107
Bst1107I GTATAC 1 cut(s) 589
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 1 cut(s) 467
Bst4CI ACNGT 1 cut(s) 616
Bst6I CTCTTC 1 cut(s) 88
BstAFI CTTAAG 1 cut(s) 743
BstDEI CTNAG 4 cut(s) 238, 449, 496, 723
BstENI CCTNNNNNAGG 1 cut(s) 499
BstKTI GATC 4 cut(s) 57, 69, 76, 655
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 4 cut(s) 54, 66, 73, 652
BstMWI GCNNNNNNNGC 2 cut(s) 137, 341
BstNI CCWGG 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 465
BstV1I GCAGC 1 cut(s) 353
BstV2I GAAGAC 1 cut(s) 674
BstX2I RGATCY 2 cut(s) 66, 652
BstYI RGATCY 2 cut(s) 66, 652
BstZ17I GTATAC 1 cut(s) 589
Bsu36I CCTNAGG 1 cut(s) 496
BsuRI GGCC 2 cut(s) 140, 470
BtsIMutI CAGTG 2 cut(s) 446, 663
CaiI CAGNNNCTG 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 202
Csp6I GTAC 1 cut(s) 617
CviAII CATG 4 cut(s) 368, 523, 638, 697
CviQI GTAC 1 cut(s) 617
DdeI CTNAG 4 cut(s) 238, 449, 496, 723
DpnI GATC 4 cut(s) 56, 68, 75, 654
DpnII GATC 4 cut(s) 54, 66, 73, 652
DriI GACNNNNNGTC 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 88
Eam1105I GACNNNNNGTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 88
EciI GGCGGA 1 cut(s) 20
Eco147I AGGCCT 1 cut(s) 470
Eco57I CTGAAG 1 cut(s) 704
Eco81I CCTNAGG 1 cut(s) 496
EcoNI CCTNNNNNAGG 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 465
FaeI CATG 4 cut(s) 371, 526, 641, 700
FaqI GGGAC 2 cut(s) 239, 518
FatI CATG 4 cut(s) 367, 522, 637, 696
FblI GTMKAC 1 cut(s) 588
Fnu4HI GCNGC 1 cut(s) 342
Fsp4HI GCNGC 1 cut(s) 342
GluI GCNGC 1 cut(s) 342
HaeIII GGCC 2 cut(s) 140, 470
HapII CCGG 1 cut(s) 203
Hin1II CATG 4 cut(s) 371, 526, 641, 700
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 7 cut(s) 38, 80, 98, 119, 307, 385, 580
HpaII CCGG 1 cut(s) 203
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 3 cut(s) 45, 97, 103
Hpy188III TCNNGA 4 cut(s) 77, 304, 401, 577
Hpy8I GTNNAC 1 cut(s) 589
Hpy99I CGWCG 2 cut(s) 91, 94
HpyAV CCTTC 4 cut(s) 130, 495, 670, 706
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4IV ACGT 1 cut(s) 671
HpyCH4V TGCA 2 cut(s) 28, 446
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 341
HpyF3I CTNAG 4 cut(s) 238, 449, 496, 723
HpySE526I ACGT 1 cut(s) 671
Hsp92II CATG 4 cut(s) 371, 526, 641, 700
Kzo9I GATC 4 cut(s) 54, 66, 73, 652
LmnI GCTCC 2 cut(s) 486, 679
Lsp1109I GCAGC 1 cut(s) 353
MaeII ACGT 1 cut(s) 671
MaeIII GTNAC 2 cut(s) 317, 606
MalI GATC 4 cut(s) 56, 68, 75, 654
MboI GATC 4 cut(s) 54, 66, 73, 652
MboII GAAGA 7 cut(s) 20, 47, 64, 75, 523, 679, 697
MflI RGATCY 2 cut(s) 66, 652
MhlI GDGCHC 1 cut(s) 108
MluCI AATT 4 cut(s) 11, 284, 396, 515
MlyI GAGTC 5 cut(s) 74, 128, 316, 379, 574
MnlI CCTC 3 cut(s) 216, 412, 634
MseI TTAA 4 cut(s) 414, 482, 744, 760
MspCI CTTAAG 1 cut(s) 743
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 467
MvaI CCWGG 1 cut(s) 467
MwoI GCNNNNNNNGC 2 cut(s) 137, 341
NdeII GATC 4 cut(s) 54, 66, 73, 652
NlaIII CATG 4 cut(s) 371, 526, 641, 700
NmuCI GTSAC 1 cut(s) 317
PceI AGGCCT 1 cut(s) 470
PfeI GAWTC 2 cut(s) 38, 98
PkrI GCNGC 1 cut(s) 343
PleI GAGTC 5 cut(s) 74, 127, 315, 379, 574
PpsI GAGTC 5 cut(s) 74, 127, 315, 379, 574
Psp6I CCWGG 1 cut(s) 465
PspGI CCWGG 1 cut(s) 465
PstNI CAGNNNCTG 1 cut(s) 245
PsuI RGATCY 2 cut(s) 66, 652
RsaI GTAC 1 cut(s) 618
RsaNI GTAC 1 cut(s) 617
SaqAI TTAA 4 cut(s) 414, 482, 744, 760
SatI GCNGC 1 cut(s) 342
Sau3AI GATC 4 cut(s) 54, 66, 73, 652
SchI GAGTC 5 cut(s) 74, 128, 316, 379, 574
ScrFI CCNGG 1 cut(s) 467
SduI GDGCHC 1 cut(s) 108
SmlI CTYRAG 2 cut(s) 304, 743
SmoI CTYRAG 2 cut(s) 304, 743
Sse9I AATT 4 cut(s) 11, 284, 396, 515
SseBI AGGCCT 1 cut(s) 470
SsiI CCGC 1 cut(s) 5
StuI AGGCCT 1 cut(s) 470
StyD4I CCNGG 1 cut(s) 465
TaaI ACNGT 1 cut(s) 616
TaiI ACGT 1 cut(s) 674
TasI AATT 4 cut(s) 11, 284, 396, 515
TfiI GAWTC 2 cut(s) 38, 98
Tru1I TTAA 4 cut(s) 414, 482, 744, 760
Tru9I TTAA 4 cut(s) 414, 482, 744, 760
TscAI CASTG 2 cut(s) 453, 663
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 1 cut(s) 341
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 2 cut(s) 167, 392
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 2 cut(s) 453, 663
Vha464I CTTAAG 1 cut(s) 743
XagI CCTNNNNNAGG 1 cut(s) 499
XapI RAATTY 2 cut(s) 396, 515
XmiI GTMKAC 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.