MD06G1095200.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002430
Physical Location & Seq
Forward (+)
22878449 .. 22880653
2205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1095200.v1.1.491

Sequence Viewer

Length: 777 bp
ATGGCGGAGCGTACGGACGGAGTCAAGGCGGCGGAGTCTTCGATGGACAGGATATCGGACAAGATCCACGGCCACGATTCGTCGTCGGCGGTGCCGTCGTCTGGGGCGGTGCACGACAAGGGCATTGGCGGTGGCCATTCAACGTCGTTGAAGTCCAAGGTTAACAGGCTCTTTGGGAGGGAGAAGCCCGTGCACCACGTCTTCGGTGGCGGAAAAGCGGCTGATGTTTTCCTGTGGAGGGACAAGAAGGCGTCGGCAGGTGTTCTTATCGCCTTAACGATCATATGGGTTTTCTTTGAATTGTTTGAATACCACCTGCTGACTCTGGTCTCCCACTTGTTGATTCTTGGGATTGCTTCGTTGTTCTTATGGTCCAACGCTTCAACCTTCATTAACAAGTCTCCACCTCACATTCCACAAGTTCAAATTCCAGAAAAGCTGGTTCTACAGATTGCGTCTGTAATCACATTGGAGATCAATCGGGCTTTTGTTATCCTGCGCGAGATTGCTTCTGGCAAGGATCTGAAGCAGTTCCTCTCTGTGATTGCTGGATTGTGGGTTGTGTCTATCTTGGGCAAGTGGTACAACTTCTTGACCTTGGTGTACATAGCGGTGGTTTTGCTCTTCACGCTGCCTGTATTCTACGAGAAATACGACGACCAGGTGGATGCAGTTGCGGAGAAGGCACTGATTGAGATCAAGAAGCAGTATGCAGTATTTGACGCGAAGGTTATGAGTAAAATTCCTAGGGGGCAATTGAAGGACAAGAAGTTTTAG

Protein Analysis

259

Amino Acids

28.63

Weight (kDa)

9.42

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 74 - 229 2.8e-56 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 248, 324
Acc36I ACCTGC 2 cut(s) 248, 324
AccB1I GGYRCC 1 cut(s) 91
AccII CGCG 2 cut(s) 501, 725
AclWI GGATC 2 cut(s) 58, 528
AcoI YGGCCR 2 cut(s) 70, 133
AcsI RAATTY 2 cut(s) 426, 741
AcuI CTGAAG 1 cut(s) 545
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 3 cut(s) 13, 584, 605
AfiI CCNNNNNNNGG 2 cut(s) 101, 238
AgsI TTSAA 7 cut(s) 141, 151, 299, 308, 384, 425, 760
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 1 cut(s) 660
AluBI AGCT 1 cut(s) 439
AluI AGCT 1 cut(s) 439
Alw21I GWGCWC 2 cut(s) 114, 195
Alw26I GTCTC 2 cut(s) 334, 405
Alw44I GTGCAC 2 cut(s) 110, 191
AlwI GGATC 2 cut(s) 58, 528
AoxI GGCC 2 cut(s) 70, 133
ApaLI GTGCAC 2 cut(s) 110, 191
ApeKI GCWGC 1 cut(s) 631
ApoI RAATTY 2 cut(s) 426, 741
Asp700I GAANNNNTTC 1 cut(s) 530
AspA2I CCTAGG 1 cut(s) 746
AspLEI GCGC 1 cut(s) 501
AspS9I GGNCC 1 cut(s) 372
AvaII GGWCC 1 cut(s) 372
AvrII CCTAGG 1 cut(s) 746
BaeGI GKGCMC 2 cut(s) 114, 195
BalI TGGCCA 1 cut(s) 135
BanI GGYRCC 1 cut(s) 91
BbsI GAAGAC 2 cut(s) 30, 193
Bbv12I GWGCWC 2 cut(s) 114, 195
BbvI GCAGC 1 cut(s) 618
BccI CCATC 1 cut(s) 37
BceAI ACGGC 2 cut(s) 79, 85
BciT130I CCWGG 1 cut(s) 662
BcoDI GTCTC 2 cut(s) 334, 405
BfaI CTAG 1 cut(s) 747
BfmI CTRYAG 1 cut(s) 446
BfuAI ACCTGC 2 cut(s) 248, 324
BisI GCNGC 3 cut(s) 30, 219, 632
BlnI CCTAGG 1 cut(s) 746
BlsI GCNGC 3 cut(s) 31, 220, 633
Bme1390I CCNGG 1 cut(s) 662
Bme18I GGWCC 1 cut(s) 372
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 372
BmiI GGNNCC 1 cut(s) 93
BmrFI CCNGG 1 cut(s) 662
BmsI GCATC 1 cut(s) 658
BoxI GACNNNNGTC 1 cut(s) 326
BpiI GAAGAC 2 cut(s) 30, 193
BsaBI GATNNNNATC 1 cut(s) 695
BsaHI GRCGYC 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 334
BsaJI CCNNGG 4 cut(s) 67, 156, 597, 746
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 238
Bse8I GATNNNNATC 1 cut(s) 695
BseBI CCWGG 1 cut(s) 662
BseDI CCNNGG 4 cut(s) 67, 156, 597, 746
BseGI GGATG 1 cut(s) 673
BseJI GATNNNNATC 1 cut(s) 695
BseLI CCNNNNNNNGG 2 cut(s) 101, 238
BseSI GKGCMC 2 cut(s) 114, 195
BseXI GCAGC 1 cut(s) 618
Bsh1236I CGCG 2 cut(s) 501, 725
BshFI GGCC 2 cut(s) 72, 135
BshNI GGYRCC 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 114, 195
BsiWI CGTACG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 2 cut(s) 101, 238
BsmAI GTCTC 2 cut(s) 334, 405
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 2 cut(s) 72, 135
Bso31I GGTCTC 1 cut(s) 334
Bsp1286I GDGCHC 2 cut(s) 114, 195
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 5 cut(s) 63, 279, 474, 520, 696
BspANI GGCC 2 cut(s) 72, 135
BspFNI CGCG 2 cut(s) 501, 725
BspLI GGNNCC 1 cut(s) 93
BspMI ACCTGC 2 cut(s) 248, 324
BspPI GGATC 2 cut(s) 58, 528
BspQI GCTCTTC 1 cut(s) 629
BspT107I GGYRCC 1 cut(s) 91
BspTNI GGTCTC 1 cut(s) 334
BsrGI TGTACA 1 cut(s) 603
BssECI CCNNGG 4 cut(s) 67, 156, 597, 746
BssMI GATC 5 cut(s) 63, 279, 474, 520, 696
BssNI GRCGYC 1 cut(s) 251
BssT1I CCWWGG 3 cut(s) 156, 597, 746
Bst2UI CCWGG 1 cut(s) 662
Bst6I CTCTTC 1 cut(s) 629
BstACI GRCGYC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 603
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 1 cut(s) 673
BstFNI CGCG 2 cut(s) 501, 725
BstHHI GCGC 1 cut(s) 501
BstKTI GATC 5 cut(s) 66, 282, 477, 523, 699
BstMAI GTCTC 2 cut(s) 334, 405
BstMBI GATC 5 cut(s) 63, 279, 474, 520, 696
BstMWI GCNNNNNNNGC 2 cut(s) 628, 683
BstNI CCWGG 1 cut(s) 662
BstPAI GACNNNNGTC 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 660
BstSFI CTRYAG 1 cut(s) 446
BstSLI GKGCMC 2 cut(s) 114, 195
BstUI CGCG 2 cut(s) 501, 725
BstV1I GCAGC 1 cut(s) 618
BstV2I GAAGAC 2 cut(s) 30, 193
BstX2I RGATCY 2 cut(s) 63, 520
BstYI RGATCY 2 cut(s) 63, 520
BsuRI GGCC 2 cut(s) 72, 135
BtgI CCRYGG 1 cut(s) 67
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 1 cut(s) 673
BtsIMutI CAGTG 1 cut(s) 686
BveI ACCTGC 2 cut(s) 248, 324
CfoI GCGC 1 cut(s) 501
Cfr13I GGNCC 1 cut(s) 372
CseI GACGC 3 cut(s) 240, 444, 731
CsiI ACCWGGT 1 cut(s) 660
Csp6I GTAC 3 cut(s) 12, 583, 604
CviJI RGCY 7 cut(s) 72, 135, 169, 187, 221, 439, 485
CviKI_1 RGCY 7 cut(s) 72, 135, 169, 187, 221, 439, 485
CviQI GTAC 3 cut(s) 12, 583, 604
DpnI GATC 5 cut(s) 65, 281, 476, 522, 698
DpnII GATC 5 cut(s) 63, 279, 474, 520, 696
EaeI YGGCCR 2 cut(s) 70, 133
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
EciI GGCGGA 3 cut(s) 20, 47, 225
Eco130I CCWWGG 3 cut(s) 156, 597, 746
Eco31I GGTCTC 1 cut(s) 334
Eco32I GATATC 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 372
Eco57I CTGAAG 1 cut(s) 545
EcoRII CCWGG 1 cut(s) 660
EcoRV GATATC 1 cut(s) 54
EcoT14I CCWWGG 3 cut(s) 156, 597, 746
ErhI CCWWGG 3 cut(s) 156, 597, 746
FaiI YATR 6 cut(s) 284, 286, 370, 608, 711, 734
FaqI GGGAC 1 cut(s) 254
FauNDI CATATG 1 cut(s) 284
Fnu4HI GCNGC 3 cut(s) 30, 219, 632
FokI GGATG 1 cut(s) 680
Fsp4HI GCNGC 3 cut(s) 30, 219, 632
FspBI CTAG 1 cut(s) 747
GlaI GCGC 1 cut(s) 500
GluI GCNGC 3 cut(s) 30, 219, 632
HaeIII GGCC 2 cut(s) 72, 135
HgaI GACGC 3 cut(s) 240, 444, 731
HhaI GCGC 1 cut(s) 501
Hin1I GRCGYC 1 cut(s) 251
Hin6I GCGC 1 cut(s) 499
HinP1I GCGC 1 cut(s) 499
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 5 cut(s) 21, 35, 77, 322, 343
HpaI GTTAAC 1 cut(s) 163
Hpy166II GTNNAC 4 cut(s) 112, 163, 193, 604
Hpy188I TCNGA 2 cut(s) 58, 525
Hpy188III TCNNGA 3 cut(s) 431, 592, 700
Hpy8I GTNNAC 4 cut(s) 112, 163, 193, 604
Hpy99I CGWCG 6 cut(s) 85, 88, 100, 148, 256, 659
HpyAV CCTTC 5 cut(s) 241, 397, 676, 721, 754
HpyCH4IV ACGT 2 cut(s) 143, 198
HpyCH4V TGCA 4 cut(s) 112, 193, 671, 713
HpyF10VI GCNNNNNNNGC 2 cut(s) 628, 683
HpySE526I ACGT 2 cut(s) 143, 198
Hsp92I GRCGYC 1 cut(s) 251
HspAI GCGC 1 cut(s) 499
KspAI GTTAAC 1 cut(s) 163
Kzo9I GATC 5 cut(s) 63, 279, 474, 520, 696
LguI GCTCTTC 1 cut(s) 629
LmnI GCTCC 1 cut(s) 7
Lsp1109I GCAGC 1 cut(s) 618
LweI GCATC 1 cut(s) 658
MabI ACCWGGT 1 cut(s) 660
MaeI CTAG 1 cut(s) 747
MaeII ACGT 2 cut(s) 143, 198
MalI GATC 5 cut(s) 65, 281, 476, 522, 698
MboI GATC 5 cut(s) 63, 279, 474, 520, 696
MboII GAAGA 3 cut(s) 30, 193, 616
MfeI CAATTG 1 cut(s) 755
MflI RGATCY 2 cut(s) 63, 520
MhlI GDGCHC 2 cut(s) 114, 195
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 4 cut(s) 299, 426, 741, 755
MluNI TGGCCA 1 cut(s) 135
MlyI GAGTC 3 cut(s) 30, 44, 316
MmeI TCCRAC 1 cut(s) 399
MnlI CCTC 4 cut(s) 171, 231, 417, 545
Mox20I TGGCCA 1 cut(s) 135
MroXI GAANNNNTTC 1 cut(s) 530
MscI TGGCCA 1 cut(s) 135
MseI TTAA 3 cut(s) 162, 275, 393
MslI CAYNNNNRTG 1 cut(s) 611
Msp20I TGGCCA 1 cut(s) 135
MspR9I CCNGG 1 cut(s) 662
MunI CAATTG 1 cut(s) 755
MvaI CCWGG 1 cut(s) 662
MvnI CGCG 2 cut(s) 501, 725
MwoI GCNNNNNNNGC 2 cut(s) 628, 683
NdeI CATATG 1 cut(s) 284
NdeII GATC 5 cut(s) 63, 279, 474, 520, 696
NlaIV GGNNCC 1 cut(s) 93
PaqCI CACCTGC 2 cut(s) 248, 324
PciSI GCTCTTC 1 cut(s) 629
PcsI WCGNNNNNNNCGW 2 cut(s) 92, 651
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 2 cut(s) 77, 343
Pfl23II CGTACG 1 cut(s) 11
PflFI GACNNNGTC 1 cut(s) 20
PkrI GCNGC 3 cut(s) 31, 220, 633
PleI GAGTC 3 cut(s) 29, 43, 316
PpsI GAGTC 3 cut(s) 29, 43, 316
PshAI GACNNNNGTC 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 660
PspGI CCWGG 1 cut(s) 660
PspLI CGTACG 1 cut(s) 11
PspN4I GGNNCC 1 cut(s) 93
PspPI GGNCC 1 cut(s) 372
PsuI RGATCY 2 cut(s) 63, 520
PsyI GACNNNGTC 1 cut(s) 20
RsaI GTAC 3 cut(s) 13, 584, 605
RsaNI GTAC 3 cut(s) 12, 583, 604
RseI CAYNNNNRTG 1 cut(s) 611
SapI GCTCTTC 1 cut(s) 629
SaqAI TTAA 3 cut(s) 162, 275, 393
SatI GCNGC 3 cut(s) 30, 219, 632
Sau3AI GATC 5 cut(s) 63, 279, 474, 520, 696
Sau96I GGNCC 1 cut(s) 372
SchI GAGTC 3 cut(s) 30, 44, 316
ScrFI CCNGG 1 cut(s) 662
SduI GDGCHC 2 cut(s) 114, 195
SexAI ACCWGGT 1 cut(s) 660
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 1 cut(s) 446
SinI GGWCC 1 cut(s) 372
SmiMI CAYNNNNRTG 1 cut(s) 611
Sse9I AATT 4 cut(s) 299, 426, 741, 755
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 1 cut(s) 660
StyI CCWWGG 3 cut(s) 156, 597, 746
TaiI ACGT 2 cut(s) 146, 201
TaqI TCGA 1 cut(s) 41
TasI AATT 4 cut(s) 299, 426, 741, 755
TatI WGTACW 1 cut(s) 603
TauI GCSGC 2 cut(s) 32, 221
TfiI GAWTC 2 cut(s) 77, 343
Tru1I TTAA 3 cut(s) 162, 275, 393
Tru9I TTAA 3 cut(s) 162, 275, 393
TscAI CASTG 1 cut(s) 693
TseI GCWGC 1 cut(s) 631
TspDTI ATGAA 1 cut(s) 379
TspGWI ACGGA 2 cut(s) 29, 33
TspRI CASTG 1 cut(s) 693
Tth111I GACNNNGTC 1 cut(s) 20
VneI GTGCAC 2 cut(s) 110, 191
VpaK11BI GGWCC 1 cut(s) 372
XapI RAATTY 2 cut(s) 426, 741
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmaJI CCTAGG 1 cut(s) 746
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.