pycom07g12740

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002430
Physical Location & Seq
Reverse (-)
14057746 .. 14059366
1621 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g12740.1

Sequence Viewer

Length: 780 bp
ATGGCCGACGAGAAGAACGCCGGATCGCCGGTGGAGCACGAGCCATCGCTGTTGGAGAAGGTAGGCGAGAAGATCCACGGCGGCCATCACCATTCCTCCTCGTCGTCTTCTTCCGACTCAGATGACGAGAAATCGGAGAAGAAGGCTGAGTCTGTGAAGACCAAGGTCTTCCGGATTTTCGGCCGGGAGAAGCCGGTTCACCATGTGCTCGGCGGTGGAAAGTCTGCTGATGTATTTTTGTGGCGAAACAAGAAGGTTTCGGGTGGGGTTATCGGCGGAGCCACCGTAGTCTGGGTCCTTTTTGAGTTGCTTCAGTACCACCTGCTTACTCTTGTTGCCCACATTTTGATTGCTACCCTTGCAATCTTGTTCCTCTGGTCCAATGCCCATACTTTTATCCACAAAACTCCTCCTCGTATTCCAGAAGTTCGTCTTCCTGAAGAACCATTCCTGCAGGTTGCATCTGAGCTGAGAGTTGTAATCAACTGTGCATTTGCACTCCTTCACAGTATTGCATCTGGGAAAGACTTGAAGAAGTTCCTTGCTGTTATTGCTGGCCTGTGGGTTCTTTCCGTTGTGGGCAAATGGTGTAACTTCCTGACCTTGTTTTACATATCCTTTGTTTTGCTGCACACCGTGCCCTTCATTTATGAGAAGTATGAAGACAAAATCGACCCACTTGCGGAGAAGGTGACAATCGAATTCAAAAAGCAGTATGCTGTCTTTGATGCCAAGGTTTTGAGTAAGATTCCGCGTGGTCTGAAAAACAAGAGGGCTTAG

Protein Analysis

260

Amino Acids

28.96

Weight (kDa)

9.25

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 76 - 231 1.8e-59 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 330
Acc36I ACCTGC 2 cut(s) 330, 445
AccII CGCG 1 cut(s) 754
AccIII TCCGGA 1 cut(s) 171
AciI CCGC 5 cut(s) 81, 213, 276, 683, 752
AclWI GGATC 2 cut(s) 31, 67
AcoI YGGCCR 3 cut(s) 3, 82, 181
AcsI RAATTY 1 cut(s) 701
AcuI CTGAAG 2 cut(s) 296, 459
AdeI CACNNNGTG 2 cut(s) 205, 637
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 2 cut(s) 291, 682
AgsI TTSAA 2 cut(s) 532, 706
AluBI AGCT 1 cut(s) 469
AluI AGCT 1 cut(s) 469
Alw21I GWGCWC 2 cut(s) 39, 210
AlwI GGATC 2 cut(s) 31, 67
Aor13HI TCCGGA 1 cut(s) 171
AoxI GGCC 4 cut(s) 3, 82, 181, 556
ApeKI GCWGC 1 cut(s) 628
ApoI RAATTY 1 cut(s) 701
Asp700I GAANNNNTTC 1 cut(s) 536
AspS9I GGNCC 2 cut(s) 295, 378
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 3 cut(s) 80, 191, 703
AvaII GGWCC 2 cut(s) 295, 378
BaeGI GKGCMC 1 cut(s) 642
BauI CACGAG 1 cut(s) 38
BbsI GAAGAC 5 cut(s) 99, 160, 164, 425, 669
Bbv12I GWGCWC 2 cut(s) 39, 210
BbvI GCAGC 1 cut(s) 615
BccI CCATC 2 cut(s) 52, 93
BceAI ACGGC 1 cut(s) 94
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BcnI CCSGG 1 cut(s) 185
BfmI CTRYAG 1 cut(s) 452
BfuAI ACCTGC 2 cut(s) 330, 445
BisI GCNGC 2 cut(s) 82, 629
BlsI GCNGC 2 cut(s) 83, 630
Bme1390I CCNGG 1 cut(s) 185
Bme18I GGWCC 2 cut(s) 295, 378
BmgT120I GGNCC 2 cut(s) 295, 378
BmiI GGNNCC 2 cut(s) 280, 296
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 3 cut(s) 470, 524, 718
BoxI GACNNNNGTC 1 cut(s) 164
BpiI GAAGAC 5 cut(s) 99, 160, 164, 425, 669
BpuMI CCSGG 1 cut(s) 185
BsaJI CCNNGG 3 cut(s) 76, 162, 732
BsaWI WCCGGW 1 cut(s) 171
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 270, 300
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 682
Bse118I RCCGGY 2 cut(s) 28, 193
BseAI TCCGGA 1 cut(s) 171
BseDI CCNNGG 3 cut(s) 76, 162, 732
BseLI CCNNNNNNNGG 2 cut(s) 291, 682
BseMII CTCAG 4 cut(s) 132, 138, 456, 461
BseRI GAGGAG 3 cut(s) 88, 399, 402
BseSI GKGCMC 1 cut(s) 642
BseX3I CGGCCG 1 cut(s) 181
BseXI GCAGC 1 cut(s) 615
BsgI GTGCAG 1 cut(s) 614
Bsh1236I CGCG 1 cut(s) 754
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 4 cut(s) 5, 84, 183, 558
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 2 cut(s) 39, 210
BsiSI CCGG 5 cut(s) 21, 29, 172, 184, 194
BslI CCNNNNNNNGG 2 cut(s) 291, 682
BsnI GGCC 4 cut(s) 5, 84, 183, 558
Bsp1286I GDGCHC 3 cut(s) 39, 210, 642
Bsp13I TCCGGA 1 cut(s) 171
Bsp143I GATC 2 cut(s) 23, 72
BspACI CCGC 5 cut(s) 81, 213, 276, 683, 752
BspANI GGCC 4 cut(s) 5, 84, 183, 558
BspCNI CTCAG 4 cut(s) 131, 139, 457, 462
BspEI TCCGGA 1 cut(s) 171
BspFNI CGCG 1 cut(s) 754
BspLI GGNNCC 2 cut(s) 280, 296
BspMAI CTGCAG 1 cut(s) 456
BspMI ACCTGC 2 cut(s) 330, 445
BspPI GGATC 2 cut(s) 31, 67
BsrFI RCCGGY 2 cut(s) 28, 193
BssAI RCCGGY 2 cut(s) 28, 193
BssECI CCNNGG 3 cut(s) 76, 162, 732
BssMI GATC 2 cut(s) 23, 72
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 162, 732
Bst2BI CACGAG 1 cut(s) 38
Bst4CI ACNGT 4 cut(s) 286, 488, 509, 637
BstAPI GCANNNNNTGC 1 cut(s) 637
BstC8I GCNNGC 1 cut(s) 556
BstDEI CTNAG 5 cut(s) 118, 147, 465, 470, 777
BstDSI CCRYGG 1 cut(s) 76
BstFNI CGCG 1 cut(s) 754
BstKTI GATC 2 cut(s) 26, 75
BstMBI GATC 2 cut(s) 23, 72
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 4 cut(s) 34, 359, 551, 637
BstPAI GACNNNNGTC 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 452
BstSLI GKGCMC 1 cut(s) 642
BstUI CGCG 1 cut(s) 754
BstV1I GCAGC 1 cut(s) 615
BstV2I GAAGAC 5 cut(s) 99, 160, 164, 425, 669
BstX2I RGATCY 1 cut(s) 72
BstYI RGATCY 1 cut(s) 72
BstZI CGGCCG 1 cut(s) 181
BsuRI GGCC 4 cut(s) 5, 84, 183, 558
BtgI CCRYGG 1 cut(s) 76
BtgZI GCGATG 1 cut(s) 30
BveI ACCTGC 2 cut(s) 330, 445
Cac8I GCNNGC 1 cut(s) 556
Cfr10I RCCGGY 2 cut(s) 28, 193
Cfr13I GGNCC 2 cut(s) 295, 378
Csp6I GTAC 1 cut(s) 316
CviAII CATG 1 cut(s) 203
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 5 cut(s) 118, 147, 465, 470, 777
DpnI GATC 2 cut(s) 25, 74
DpnII GATC 2 cut(s) 23, 72
DraIII CACNNNGTG 2 cut(s) 205, 637
EaeI YGGCCR 3 cut(s) 3, 82, 181
EagI CGGCCG 1 cut(s) 181
EciI GGCGGA 1 cut(s) 291
EclXI CGGCCG 1 cut(s) 181
Eco130I CCWWGG 2 cut(s) 162, 732
Eco47I GGWCC 2 cut(s) 295, 378
Eco52I CGGCCG 1 cut(s) 181
Eco57I CTGAAG 2 cut(s) 296, 459
EcoO109I RGGNCCY 1 cut(s) 295
EcoRI GAATTC 1 cut(s) 701
EcoT14I CCWWGG 2 cut(s) 162, 732
ErhI CCWWGG 2 cut(s) 162, 732
FaeI CATG 1 cut(s) 206
FaiI YATR 6 cut(s) 204, 390, 614, 651, 660, 717
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FatI CATG 1 cut(s) 202
Fnu4HI GCNGC 2 cut(s) 82, 629
Fsp4HI GCNGC 2 cut(s) 82, 629
GluI GCNGC 2 cut(s) 82, 629
HaeIII GGCC 4 cut(s) 5, 84, 183, 558
HapII CCGG 5 cut(s) 21, 29, 172, 184, 194
Hin1II CATG 1 cut(s) 206
HinfI GANTC 3 cut(s) 116, 149, 748
HpaII CCGG 5 cut(s) 21, 29, 172, 184, 194
HphI GGTGA 3 cut(s) 80, 191, 703
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 5 cut(s) 115, 121, 136, 466, 762
Hpy188III TCNNGA 4 cut(s) 172, 422, 437, 598
Hpy8I GTNNAC 1 cut(s) 199
Hpy99I CGWCG 2 cut(s) 11, 106
HpyAV CCTTC 6 cut(s) 52, 136, 247, 512, 652, 682
HpyCH4III ACNGT 4 cut(s) 286, 488, 509, 637
HpyCH4V TGCA 7 cut(s) 362, 454, 461, 491, 497, 515, 631
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 359, 551, 637
HpyF3I CTNAG 5 cut(s) 118, 147, 465, 470, 777
Hsp92II CATG 1 cut(s) 206
Kpn2I TCCGGA 1 cut(s) 171
Kzo9I GATC 2 cut(s) 23, 72
LmnI GCTCC 2 cut(s) 34, 278
Lsp1109I GCAGC 1 cut(s) 615
LweI GCATC 3 cut(s) 470, 524, 718
MaeIII GTNAC 2 cut(s) 590, 691
MalI GATC 2 cut(s) 25, 74
MboI GATC 2 cut(s) 23, 72
MflI RGATCY 1 cut(s) 72
MhlI GDGCHC 3 cut(s) 39, 210, 642
MluCI AATT 1 cut(s) 701
MlyI GAGTC 2 cut(s) 110, 158
MmeI TCCRAC 2 cut(s) 33, 138
MnlI CCTC 6 cut(s) 106, 109, 383, 420, 423, 765
MroI TCCGGA 1 cut(s) 171
MroXI GAANNNNTTC 1 cut(s) 536
MspI CCGG 5 cut(s) 21, 29, 172, 184, 194
MspR9I CCNGG 1 cut(s) 185
MvnI CGCG 1 cut(s) 754
MwoI GCNNNNNNNGC 4 cut(s) 34, 359, 551, 637
NciI CCSGG 1 cut(s) 185
NdeII GATC 2 cut(s) 23, 72
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 2 cut(s) 280, 296
NmeAIII GCCGAG 1 cut(s) 189
NmuCI GTSAC 1 cut(s) 691
PaqCI CACCTGC 1 cut(s) 330
PdmI GAANNNNTTC 1 cut(s) 536
PfeI GAWTC 1 cut(s) 748
PkrI GCNGC 2 cut(s) 83, 630
PleI GAGTC 2 cut(s) 110, 157
PpsI GAGTC 2 cut(s) 110, 157
PpuMI RGGWCCY 1 cut(s) 295
PshAI GACNNNNGTC 1 cut(s) 164
Psp5II RGGWCCY 1 cut(s) 295
PspN4I GGNNCC 2 cut(s) 280, 296
PspPI GGNCC 2 cut(s) 295, 378
PspPPI RGGWCCY 1 cut(s) 295
PstI CTGCAG 1 cut(s) 456
PsuI RGATCY 1 cut(s) 72
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
SatI GCNGC 2 cut(s) 82, 629
Sau3AI GATC 2 cut(s) 23, 72
Sau96I GGNCC 2 cut(s) 295, 378
SbfI CCTGCAGG 1 cut(s) 456
SchI GAGTC 2 cut(s) 110, 158
ScrFI CCNGG 1 cut(s) 185
SdaI CCTGCAGG 1 cut(s) 456
SduI GDGCHC 3 cut(s) 39, 210, 642
SetI ASST 9 cut(s) 63, 168, 258, 324, 459, 471, 605, 693, 738
SfaNI GCATC 3 cut(s) 470, 524, 718
SfcI CTRYAG 1 cut(s) 452
SgrAI CRCCGGYG 1 cut(s) 28
SinI GGWCC 2 cut(s) 295, 378
Sse8387I CCTGCAGG 1 cut(s) 456
Sse9I AATT 1 cut(s) 701
SsiI CCGC 5 cut(s) 81, 213, 276, 683, 752
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 2 cut(s) 162, 732
TaaI ACNGT 4 cut(s) 286, 488, 509, 637
TaqI TCGA 2 cut(s) 672, 699
TasI AATT 1 cut(s) 701
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 1 cut(s) 748
TseFI GTSAC 1 cut(s) 691
TseI GCWGC 1 cut(s) 628
Tsp45I GTSAC 1 cut(s) 691
TspDTI ATGAA 2 cut(s) 634, 675
TspGWI ACGGA 1 cut(s) 562
VpaK11BI GGWCC 2 cut(s) 295, 378
XapI RAATTY 1 cut(s) 701
XmnI GAANNNNTTC 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.