Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0002430

Physical Location & Seq

Genomic Coordinates Forward (+)

2206957 .. 2215836

Gene Structure 8880 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0031700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3783 bp

ATGGGCGGCGACCGTCACGACACCACTCGGCCGGACTGTTCCTCGTCTTCTTCTTCTTCTTCTTCGTCGTCTTCGTCTTCCGAGTCGCGAGTCCGGCTCGCTCAGACGCCGATTCTGTTGCTGGTTTGCTTCCACAAGGCGATGCGAGCCGAGCTCGCCGATCTCCGCCACGTCACCGAGGCCGCCTTGGACGGTGGATTCAGAGGCCGCGTTGTTCTCGAGGTTCTGCGACGGTTTGAGTTCCTCAAGCTCGCGTATAAGTACCACTGCTCTGCTGAGGATGAGGTTATTTTTCTAGCGCTAGATGGACGAACAAAAAACATAGCTTGTACATATTCATTGGAGCATAGGAGTATTGATGGTCTTTTTGATTCTATTTTTAGTTGTTTGGATGTATTGTTAGAGGAAAGTGAAGATACTTCAAAGGAATTTCAGGAACTTGTGTTCTGCATCGGGACGCTCCAGGCGTTCATTTGCCAGCATATGGTGAAAGAAGAAGAACAGGTTTTTCCTTTGCTCCTGCAGCAATTCTCTGTGAAGGAGCAGGCTTCACTTGTGTGGCAGTTCATGTGTAGTATGCCTTTAGTGCTGCTAGAGGATCTTCTACCATGGATGATCTCCTCTCTTCAAAATGATGAAAAAGAGGAACTCATACATTGCATAAAAGAAATTGTACCTGATGAAAAACATCTCCAAGAGGTAGTAAGCTCGTGGCTTGCTAACAAGGGCCAAGCTCGACACACTGGTGATGATGAATATGCAGATATGAAAAAGTTACTCAAGTCTCATTCCCCTAAGAGGTTCTTTGAAGAAAATTGGAGTTGCATGAAGGAAGACAGCATCCAAACTGATACTGGATACAGTCCTGTTGATGGGCTTCATCTTTGGCATGGTGCCATAAGGAAAGATCTAGAAAAAATTATGGGAGAGCTATATCAATTGAGAAGCTCAGTCAATTTTCTAGATATTGATTCAATAGTTGTCCAGCTAAAGTTTCTTGCTGATGTCCTTACTTTCTACAGCAATGCATTGGAGAAGTTAGTTCACCCTGTGTTAAATGAACTTGTCAATGGCTGTCTGTACCCCACAATTGAGATATTTCCCGATGAAAGCCTTCTGGAAAGTTTGCAGAATTTACTATACTATGGCCTTGAAAATAGAACACCTTTGAGCAAATTTGTTGAAAAGCTTTGCTGGGAATTTGAGTGTCTTGTGGTGGGAGTCCGCAAGCATTTTGCTTTCCACGAAACAGAGGTATTTCCCATTATAAGGAAGAACTGTAGCCATGAAATGCAACAGCAACTCGTGTATGTCAGCCTTCATATTATGCCACTTGGGTTGCTGAAGTGTGTGACTACTTGGTTTTCAGCTAACTTATCCGAGGATGAGTCCAGGTCAATTCTTAGCAGCATGAAGCAGCGAGATTTCTTGGTTAATGAATCTTTTATGTCCCTGTTACATGAGTGGTTCCGTATTGGCCATTCAGGTAAAACCTCAATTGAAAATTTCCGAGAGGACTTGCAGCAAATATTTAAGAGCAGATGCACTTTCTTATGCAATCAATTACATGCCAGTACTGCGTGTTCATCTGTAAGCTCCAATATGCAACATCGAGGGAAATCAAACACTGGGGTAATGGAACTAATTTCTTCAAAAAAGGGAAAGAACTCTATGCCATACTCTTCATCTTTTGCCTCCGACTCTTCTACATATTCTGAGACGTCAAACTCAAGAGGAATCAATCCCCAAGTATACTTCTCTGGAATGAAAACATCATATCATATTCCTGAAAGTCTTAGTGGAGAAAACTTGAGTGGTTACGGTCTCCATGAACCAAAACCAATAGATCTCTTATTTTTCTTCCACAAGGCTCTCAGGAAGGATTTGGATTACCTTGTCCTTGGTTCAGCTGAGTTGGCTGAAAATGCAGCTTCCCTCACAGATTTTTGCCGGCGCTTTAATCTCATACAGTTCTTACATCAAATACATAGTGAAGCAGAGGACGAGGTTGCCTTTCCAGCCTTGGAGGCAAAGGGAAAATGTCAAAACATTAGCCAGTCTTACACAATGGACCACAAACTAGAGGTTGAACTCTTCCAAAAAATATCCTTCATTCTAGATGAGATGTCTAAATTGCATTTTTCAGTTTCTATGGTTGATTCAAACACAATGGACCAGATGTCGCCAAAGCACTATCAGTTGTGCATGAGGCTTCATGGTATGTGCAAATCAACGTGCAAATTACTGACTGAACATATTCATCGTGAAGAAGTTGAACTTTGGCCCTTGTTTAAAGAATGCTTCTCCATTGAAGAGCAAGAAAGGATCGTGGGATGCATACTTGGTAGAACAGAAGCAAAAATATTACAAGATATGATACCCTGGCTGATGGAATCTTTGACACCAGAAGAACAGCATGCCATGATATCTATATGGCGCCAGGTCACCAGAAACACAATGTTTGATGAGTGGTTGAAAGAATGGTGGGAGGGTTATGATGCAGGCAAGGTGGTGGAGGAGTCAAGCCTTCCTCCTTCAAAGACTGTAGATCCCTTGGAGGTTGTCTCTACGTACTTGTGTGGACTGGATGAACTAGAAAGAAAACTCTGCAACAGAAGCATCAACTTTCCTGAAAAAGATTCCCCTGATACTAATACTAAGCTGTTACAAATCAGTGAGGTGGATCATAAGCTGAGGGATGCTGATAGTCACCAATGTAAATACAACCCTAGAGAATCTGGAAGTCTTGCAGAAGGTAAGAAGATGAAAAATGAAGACATTGAAAATGCCACAGATCAAATCAATGATCCAGGACAACTTTTTCAAGCAAGTCATAAAACAGATTACTGTGAGTGCCTGCTGACACTAAGTCAAGAGGATCTGTTGACTGCAATAAGTAAAATCTCCCGCGACTCTTCCTTGGATCCTCAGAAGAAACCATATATGATGCAGAACCTGCTATCGAGCCATTGGAGAGTCAGACAACACTCTCAGTCAACTGTTGCAAGTAATGGGAAAGAATTCCCTGATCAGCATCCGTCGTATCAGGACCCATTTGGGCTAACATTTGGTTGTAAACACTATAAGAGGAACTGTAAGCTTGTTGCTGCATGTTGCAACCAGCTCTACACTTGCATACGCTGCCATGATGAGATGGCCGAGCACACAATAGATAGGAGATCTGTAACAGAGATGATGTGCATGAAATGCTTGAAAATTCAGCCAATCGGGCCCACATGCTCATCTGCATCCTGCAATGATTTATCCATGGCAAGATACTTTTGCAAAATCTGCAAAATTTTTGATGATGAAAGGATTATCTACCATTGCCCTTACTGTAACTTGTGCCGAGTTGGAAAGGGATTGGGTATTGACTACTTCCATTGCATGACTTGTAATGCTTGCATGTCCCGTTCTCTCTTCAAGCATACATGCAGAGAGAAAAGCTTTATGGATAATTGCCCTATTTGCCACGAGGACATCTTCACGTCCAACTCTCCAGTAAAGGCCCTTCCATGTGGTCATTCAATGCATTCAACATGTTTTCAGGCCTACACCTTTACTAATTATACCTGCCCAATCTGTGGCAAGTCACTTGGGGACATGCAGATGTTATTTAGAATGTATGATGCATATTTGGCTGGAGAGAAGCTTCCAGATGAGTATTCTGGCCGAACTGAGGCCATACTATGCAATGACTGTGAGAAGAAAGGAACTGCTCCCTTTCATTGGCTTTACCACAAGTGCACCTTTTGTGGTTCATACAATACCAGGCTTCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005789 GO:0005794 GO:0005886 GO:0005911 GO:0006996 GO:0007029 GO:0008150 GO:0009506 GO:0009987 GO:0010256 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0030054 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0071458 GO:0071782 GO:0071786 GO:0071840 GO:0071944 GO:0098552 GO:0098554 GO:0098562 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko05010 map05010

Pfam Domains

Protein Families

Reticulon

Protein Analysis

1260

Amino Acids

144.43

Weight (kDa)

5.74

Isoelectric Point (pI)

50.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hemerythrin	PF01814	43 - 173	5e-08	Hemerythrin HHE cation binding domain
zf-CHY	PF05495	1025 - 1093	7.4e-17	CHY zinc finger
zf-RING_2	PF13639	1152 - 1195	3.7e-09	Ring finger domain
zinc_ribbon_6	PF14599	1201 - 1258	1e-21	Zinc-ribbon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002430)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46170 AT2G46170 AT3G61560 AT3G61560 AT3G61560
fragaria_vesca	FvH4_5g03500 FvH4_5g03500 FvH4_5g03500 FvH4_7g15520
malus_domestica	MD01G1072500.v1.1 MD06G1095200.v1.1 MD07G1130400.v1.1 MD14G1115200.v1.1
prunus_persica	Prupe.2G178800_v2.0.a1 Prupe.2G178800_v2.0.a1 Prupe.2G178800_v2.0.a1 Prupe.5G110100_v2.0.a1
pyrus_communis	pycom01g10170 pycom06g09180 pycom07g12740
rosa_chinensis	RchiOBHm_Chr1g0357851 RchiOBHm_Chr7g0196181
rosa_laevigata	RLG00000004055 RLG00000027991
rosa_multiflora	Rmu_sc0002071.1_g000003 Rmu_sc0011198.1_g000005 Rmu_sc0015956.1_g000002 Rmu_sc0019298.1_g000004
rosa_roxburghii	Rroxscaffold_3G00259690 Rroxscaffold_4G00298450
rosa_rugosa	Rorug01G0259000 Rorug01G0259100 Rorug07G0031700
rosa_samantha	Rh1AG273000 Rh1BG240300 Rh7AG157300 Rh7BG158900 Rh7CG162800 Rh7DG158300
rosa_wichuraiana	Rw1G024380 Rw7G013450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1268
AatII	GACGTC	1 cut(s)	1724
Acc36I	ACCTGC	2 cut(s)	2964, 3581
AccB1I	GGYRCC	2 cut(s)	893, 2436
AccB7I	CCANNNNNTGG	2 cut(s)	484, 3584
AccI	GTMKAC	1 cut(s)	1752
AccII	CGCG	4 cut(s)	88, 210, 254, 2910
AciI	CCGC	6 cut(s)	6, 166, 183, 208, 1225, 2908
AclWI	GGATC	8 cut(s)	606, 2333, 2543, 2691, 2801, 2886, 2918, 2931
AcoI	YGGCCR	4 cut(s)	29, 1477, 3156, 3670
AcsI	RAATTY	8 cut(s)	428, 1132, 1175, 1199, 1504, 3020, 3216, 3297
AcuI	CTGAAG	1 cut(s)	1364
AcyI	GRCGYC	3 cut(s)	107, 1721, 2437
AdeI	CACNNNGTG	1 cut(s)	1051
AfaI	GTAC	6 cut(s)	263, 331, 675, 1082, 1576, 2573
AfeI	AGCGCT	1 cut(s)	300
AfiI	CCNNNNNNNGG	7 cut(s)	484, 798, 872, 1269, 3584, 3679, 3729
AflIII	ACRYGT	1 cut(s)	3539
AhdI	GACNNNNNGTC	2 cut(s)	2179, 2868
AjiI	CACGTC	2 cut(s)	172, 3489
AjnI	CCWGG	6 cut(s)	462, 1391, 2381, 2439, 2809, 3770
AleI	CACNNNNGTG	2 cut(s)	556, 744
AloI	GAACNNNNNNTCC	2 cut(s)	428, 460
Alw21I	GWGCWC	3 cut(s)	156, 3165, 3749
Alw26I	GTCTC	4 cut(s)	789, 1712, 1829, 2569
Alw44I	GTGCAC	1 cut(s)	3745
AlwI	GGATC	8 cut(s)	606, 2333, 2543, 2691, 2801, 2886, 2918, 2931
AlwNI	CAGNNNCTG	1 cut(s)	2956
Ama87I	CYCGRG	1 cut(s)	218
Aor51HI	AGCGCT	1 cut(s)	300
ApaI	GGGCCC	1 cut(s)	3234
ApaLI	GTGCAC	1 cut(s)	3745
ApeKI	GCWGC	8 cut(s)	523, 589, 1407, 1417, 1522, 1928, 3107, 3141
ApoI	RAATTY	8 cut(s)	428, 1132, 1175, 1199, 1504, 3020, 3216, 3297
Asp700I	GAANNNNTTC	3 cut(s)	1113, 2256, 3020
AspLEI	GCGC	3 cut(s)	301, 1956, 2439
AspS9I	GGNCC	8 cut(s)	727, 2071, 2173, 2283, 3049, 3230, 3231, 3508
AsuHPI	GGTGA	6 cut(s)	166, 499, 758, 1037, 2437, 2702
AvaI	CYCGRG	1 cut(s)	218
AvaII	GGWCC	3 cut(s)	2071, 2173, 3049
BaeGI	GKGCMC	2 cut(s)	3234, 3749
BalI	TGGCCA	1 cut(s)	1479
BamHI	GGATCC	1 cut(s)	2923
BanI	GGYRCC	2 cut(s)	893, 2436
BanII	GRGCYC	2 cut(s)	156, 3234
BarI	GAAGNNNNNNTAC	2 cut(s)	3495, 3527
BauI	CACGAG	3 cut(s)	709, 1304, 3473
BbsI	GAAGAC	5 cut(s)	39, 63, 69, 840, 2781
Bbv12I	GWGCWC	3 cut(s)	156, 3165, 3749
BbvCI	CCTCAGC	2 cut(s)	276, 2693
BbvI	GCAGC	8 cut(s)	535, 576, 1419, 1429, 1534, 1940, 3094, 3128
BccI	CCATC	5 cut(s)	299, 353, 866, 2383, 3148
BcgI	CGANNNNNNTGC	2 cut(s)	100, 134
BciT130I	CCWGG	6 cut(s)	464, 1393, 2383, 2441, 2811, 3772
BciVI	GTATCC	1 cut(s)	851
BclI	TGATCA	1 cut(s)	3028
BcoDI	GTCTC	4 cut(s)	789, 1712, 1829, 2569
BfaI	CTAG	9 cut(s)	296, 302, 593, 911, 962, 2081, 2117, 2594, 2730
BfmI	CTRYAG	4 cut(s)	521, 1018, 1279, 2544
BfoI	RGCGCY	3 cut(s)	302, 1957, 2440
BfuAI	ACCTGC	2 cut(s)	2964, 3581
BfuI	GTATCC	1 cut(s)	851
BglI	GCCNNNNNGGC	2 cut(s)	2027, 3229
BglII	AGATCT	3 cut(s)	907, 1846, 3179
BmcAI	AGTACT	1 cut(s)	1576
Bme1390I	CCNGG	6 cut(s)	464, 1393, 2383, 2441, 2811, 3772
Bme18I	GGWCC	3 cut(s)	2071, 2173, 3049
BmeRI	GACNNNNNGTC	2 cut(s)	2179, 2868
BmeT110I	CYCGRG	1 cut(s)	218
BmgBI	CACGTC	2 cut(s)	172, 3489
BmgT120I	GGNCC	8 cut(s)	727, 2071, 2173, 2283, 3049, 3230, 3231, 3508
BmiI	GGNNCC	6 cut(s)	895, 1469, 2438, 2925, 3051, 3232
BmrFI	CCNGG	6 cut(s)	464, 1393, 2383, 2441, 2811, 3772
BmrI	ACTGGG	1 cut(s)	1638
BmuI	ACTGGG	1 cut(s)	1638
BpiI	GAAGAC	5 cut(s)	39, 63, 69, 840, 2781
BplI	GAGNNNNNCTC	6 cut(s)	81, 113, 138, 170, 2549, 2581
BpmI	CTGGAG	3 cut(s)	446, 3483, 3663
Bpu10I	CCTNAGC	2 cut(s)	276, 2693
BpuEI	CTTGAG	4 cut(s)	230, 764, 1714, 1831
BsaAI	YACGTR	1 cut(s)	2571
BsaBI	GATNNNNATC	1 cut(s)	3033
BsaHI	GRCGYC	3 cut(s)	107, 1721, 2437
BsaI	GGTCTC	1 cut(s)	1829
Bsc4I	CCNNNNNNNGG	7 cut(s)	484, 798, 872, 1269, 3584, 3679, 3729
Bse118I	RCCGGY	1 cut(s)	1950
Bse1I	ACTGG	7 cut(s)	748, 859, 1572, 1633, 2056, 2589, 3500
Bse3DI	GCAATG	6 cut(s)	655, 1030, 3262, 3325, 3382, 3700
Bse8I	GATNNNNATC	1 cut(s)	3033
BseBI	CCWGG	6 cut(s)	464, 1393, 2383, 2441, 2811, 3772
BseJI	GATNNNNATC	1 cut(s)	3033
BseLI	CCNNNNNNNGG	7 cut(s)	484, 798, 872, 1269, 3584, 3679, 3729
BseMI	GCAATG	6 cut(s)	655, 1030, 3262, 3325, 3382, 3700
BseNI	ACTGG	7 cut(s)	748, 859, 1572, 1633, 2056, 2589, 3500
BseRI	GAGGAG	2 cut(s)	610, 2531
BseSI	GKGCMC	2 cut(s)	3234, 3749
BseX3I	CGGCCG	1 cut(s)	29
BseXI	GCAGC	8 cut(s)	535, 576, 1419, 1429, 1534, 1940, 3094, 3128
BseYI	CCCAGC	1 cut(s)	1194
Bsh1236I	CGCG	4 cut(s)	88, 210, 254, 2910
Bsh1285I	CGRYCG	2 cut(s)	13, 32
BshNI	GGYRCC	2 cut(s)	893, 2436
BsiEI	CGRYCG	2 cut(s)	13, 32
BsiHKAI	GWGCWC	3 cut(s)	156, 3165, 3749
BsiHKCI	CYCGRG	1 cut(s)	218
BsiSI	CCGG	3 cut(s)	32, 94, 1951
BslFI	GGGAC	4 cut(s)	469, 1435, 3394, 3614
BslI	CCNNNNNNNGG	7 cut(s)	484, 798, 872, 1269, 3584, 3679, 3729
BsmAI	GTCTC	4 cut(s)	789, 1712, 1829, 2569
BsmBI	CGTCTC	1 cut(s)	1712
BsmFI	GGGAC	4 cut(s)	469, 1435, 3394, 3614
BsmI	GAATGC	2 cut(s)	2303, 3532
Bso31I	GGTCTC	1 cut(s)	1829
BsoBI	CYCGRG	1 cut(s)	218
Bsp120I	GGGCCC	1 cut(s)	3230
Bsp1286I	GDGCHC	4 cut(s)	156, 3165, 3234, 3749
Bsp1407I	TGTACA	1 cut(s)	329
Bsp19I	CCATGG	2 cut(s)	608, 3267
Bsp68I	TCGCGA	1 cut(s)	88
BspACI	CCGC	6 cut(s)	6, 166, 183, 208, 1225, 2908
BspFNI	CGCG	4 cut(s)	88, 210, 254, 2910
BspLI	GGNNCC	6 cut(s)	895, 1469, 2438, 2925, 3051, 3232
BspMAI	CTGCAG	1 cut(s)	525
BspMI	ACCTGC	2 cut(s)	2964, 3581
BspPI	GGATC	8 cut(s)	606, 2333, 2543, 2691, 2801, 2886, 2918, 2931
BspQI	GCTCTTC	1 cut(s)	2307
BspT107I	GGYRCC	2 cut(s)	893, 2436
BspTNI	GGTCTC	1 cut(s)	1829
BsrDI	GCAATG	6 cut(s)	655, 1030, 3262, 3325, 3382, 3700
BsrFI	RCCGGY	1 cut(s)	1950
BsrGI	TGTACA	1 cut(s)	329
BsrI	ACTGG	7 cut(s)	748, 859, 1572, 1633, 2056, 2589, 3500
BssAI	RCCGGY	1 cut(s)	1950
BssNAI	GTATAC	1 cut(s)	1753
BssNI	GRCGYC	3 cut(s)	107, 1721, 2437
BssSI	CACGAG	3 cut(s)	709, 1304, 3473
BssT1I	CCWWGG	7 cut(s)	186, 608, 1900, 2022, 2553, 2919, 3267
Bst1107I	GTATAC	1 cut(s)	1753
Bst2BI	CACGAG	3 cut(s)	709, 1304, 3473
Bst2UI	CCWGG	6 cut(s)	464, 1393, 2383, 2441, 2811, 3772
Bst6I	CTCTTC	7 cut(s)	630, 1687, 1708, 2099, 2307, 2920, 3425
BstACI	GRCGYC	3 cut(s)	107, 1721, 2437
BstAPI	GCANNNNNTGC	4 cut(s)	2956, 3141, 3207, 3291
BstAUI	TGTACA	1 cut(s)	329
BstBAI	YACGTR	1 cut(s)	2571
BstDSI	CCRYGG	2 cut(s)	608, 3267
BstEII	GGTNACC	1 cut(s)	2443
BstFNI	CGCG	4 cut(s)	88, 210, 254, 2910
BstH2I	RGCGCY	3 cut(s)	302, 1957, 2440
BstHHI	GCGC	3 cut(s)	301, 1956, 2439
BstMAI	GTCTC	4 cut(s)	789, 1712, 1829, 2569
BstMCI	CGRYCG	2 cut(s)	13, 32
BstNI	CCWGG	6 cut(s)	464, 1393, 2383, 2441, 2811, 3772
BstNSI	RCATGY	8 cut(s)	1571, 2420, 3114, 3240, 3409, 3435, 3543, 3607
BstPI	GGTNACC	1 cut(s)	2443
BstSCI	CCNGG	6 cut(s)	462, 1391, 2381, 2439, 2809, 3770
BstSFI	CTRYAG	4 cut(s)	521, 1018, 1279, 2544
BstSLI	GKGCMC	2 cut(s)	3234, 3749
BstSNI	TACGTA	1 cut(s)	2571
BstUI	CGCG	4 cut(s)	88, 210, 254, 2910
BstV1I	GCAGC	8 cut(s)	535, 576, 1419, 1429, 1534, 1940, 3094, 3128
BstV2I	GAAGAC	5 cut(s)	39, 63, 69, 840, 2781
BstX2I	RGATCY	7 cut(s)	598, 907, 1846, 2548, 2878, 2923, 3179
BstYI	RGATCY	7 cut(s)	598, 907, 1846, 2548, 2878, 2923, 3179
BstZ17I	GTATAC	1 cut(s)	1753
BstZI	CGGCCG	1 cut(s)	29
BsuI	GTATCC	1 cut(s)	851
BtgI	CCRYGG	2 cut(s)	608, 3267
BtgZI	GCGATG	1 cut(s)	155
BtrI	CACGTC	2 cut(s)	172, 3489
BtsI	GCAGTG	1 cut(s)	265
BtsIMutI	CAGTG	4 cut(s)	265, 741, 1626, 2680
BtuMI	TCGCGA	1 cut(s)	88
BveI	ACCTGC	2 cut(s)	2964, 3581
CaiI	CAGNNNCTG	1 cut(s)	2956
CfoI	GCGC	3 cut(s)	301, 1956, 2439
Cfr10I	RCCGGY	1 cut(s)	1950
Cfr13I	GGNCC	8 cut(s)	727, 2071, 2173, 2283, 3049, 3230, 3231, 3508
CseI	GACGC	2 cut(s)	115, 466
Csp6I	GTAC	6 cut(s)	262, 330, 674, 1081, 1575, 2572
CspCI	CAANNNNNGTGG	2 cut(s)	1320, 1355
CviQI	GTAC	6 cut(s)	262, 330, 674, 1081, 1575, 2572
DinI	GGCGCC	1 cut(s)	2438
DraI	TTTAAA	1 cut(s)	2293
DraIII	CACNNNGTG	1 cut(s)	1051
DriI	GACNNNNNGTC	2 cut(s)	2179, 2868
EaeI	YGGCCR	4 cut(s)	29, 1477, 3156, 3670
EagI	CGGCCG	1 cut(s)	29
Eam1104I	CTCTTC	7 cut(s)	630, 1687, 1708, 2099, 2307, 2920, 3425
Eam1105I	GACNNNNNGTC	2 cut(s)	2179, 2868
EarI	CTCTTC	7 cut(s)	630, 1687, 1708, 2099, 2307, 2920, 3425
EciI	GGCGGA	1 cut(s)	155
Ecl136II	GAGCTC	1 cut(s)	154
EclXI	CGGCCG	1 cut(s)	29
Eco105I	TACGTA	1 cut(s)	2571
Eco130I	CCWWGG	7 cut(s)	186, 608, 1900, 2022, 2553, 2919, 3267
Eco147I	AGGCCT	1 cut(s)	3551
Eco24I	GRGCYC	2 cut(s)	156, 3234
Eco31I	GGTCTC	1 cut(s)	1829
Eco32I	GATATC	1 cut(s)	2427
Eco47I	GGWCC	3 cut(s)	2071, 2173, 3049
Eco47III	AGCGCT	1 cut(s)	300
Eco52I	CGGCCG	1 cut(s)	29
Eco53kI	GAGCTC	1 cut(s)	154
Eco57I	CTGAAG	1 cut(s)	1364
Eco88I	CYCGRG	1 cut(s)	218
Eco91I	GGTNACC	1 cut(s)	2443
EcoICRI	GAGCTC	1 cut(s)	154
EcoO109I	RGGNCCY	2 cut(s)	3049, 3508
EcoO65I	GGTNACC	1 cut(s)	2443
EcoRI	GAATTC	1 cut(s)	3020
EcoRII	CCWGG	6 cut(s)	462, 1391, 2381, 2439, 2809, 3770
EcoRV	GATATC	1 cut(s)	2427
EcoT14I	CCWWGG	7 cut(s)	186, 608, 1900, 2022, 2553, 2919, 3267
EcoT22I	ATGCAT	4 cut(s)	1030, 2339, 3534, 3634
EcoT38I	GRGCYC	2 cut(s)	156, 3234
EgeI	GGCGCC	1 cut(s)	2438
EheI	GGCGCC	1 cut(s)	2438
ErhI	CCWWGG	7 cut(s)	186, 608, 1900, 2022, 2553, 2919, 3267
Esp3I	CGTCTC	1 cut(s)	1712
FalI	AAGNNNNNCTT	6 cut(s)	788, 820, 2262, 2294, 3734, 3766
FaqI	GGGAC	4 cut(s)	469, 1435, 3394, 3614
FauI	CCCGC	1 cut(s)	2915
FauNDI	CATATG	1 cut(s)	483
FbaI	TGATCA	1 cut(s)	3028
FblI	GTMKAC	1 cut(s)	1752
FriOI	GRGCYC	2 cut(s)	156, 3234
FspBI	CTAG	9 cut(s)	296, 302, 593, 911, 962, 2081, 2117, 2594, 2730
GlaI	GCGC	3 cut(s)	300, 1955, 2438
GsaI	CCCAGC	1 cut(s)	1198
GsuI	CTGGAG	3 cut(s)	446, 3483, 3663
HaeII	RGCGCY	3 cut(s)	302, 1957, 2440
HapII	CCGG	3 cut(s)	32, 94, 1951
HgaI	GACGC	2 cut(s)	115, 466
HhaI	GCGC	3 cut(s)	301, 1956, 2439
Hin1I	GRCGYC	3 cut(s)	107, 1721, 2437
Hin6I	GCGC	3 cut(s)	299, 1954, 2437
HinP1I	GCGC	3 cut(s)	299, 1954, 2437
HincII	GTYRAC	2 cut(s)	2886, 2997
HindII	GTYRAC	2 cut(s)	2886, 2997
HindIII	AAGCTT	4 cut(s)	1187, 3098, 3445, 3650
HpaII	CCGG	3 cut(s)	32, 94, 1951
HphI	GGTGA	6 cut(s)	166, 499, 758, 1037, 2437, 2702
Hpy166II	GTNNAC	7 cut(s)	1045, 1753, 2582, 2886, 2997, 3077, 3747
Hpy188I	TCNGA	9 cut(s)	82, 105, 203, 1381, 1511, 1699, 1717, 2931, 2981
Hpy8I	GTNNAC	7 cut(s)	1045, 1753, 2582, 2886, 2997, 3077, 3747
Hpy99I	CGWCG	3 cut(s)	70, 234, 3043
HpyCH4IV	ACGT	5 cut(s)	171, 1721, 2234, 2570, 3488
HpySE526I	ACGT	5 cut(s)	171, 1721, 2234, 2570, 3488
Hsp92I	GRCGYC	3 cut(s)	107, 1721, 2437
HspAI	GCGC	3 cut(s)	299, 1954, 2437
KasI	GGCGCC	1 cut(s)	2436
KroI	GCCGGC	1 cut(s)	1950
KroNI	GCCGGC	1 cut(s)	1952
Ksp22I	TGATCA	1 cut(s)	3028
LguI	GCTCTTC	1 cut(s)	2307
LmnI	GCTCC	6 cut(s)	343, 465, 522, 541, 1601, 3724
Lsp1109I	GCAGC	8 cut(s)	535, 576, 1419, 1429, 1534, 1940, 3094, 3128
MaeI	CTAG	9 cut(s)	296, 302, 593, 911, 962, 2081, 2117, 2594, 2730
MaeII	ACGT	5 cut(s)	171, 1721, 2234, 2570, 3488
MfeI	CAATTG	3 cut(s)	938, 1089, 1497
MflI	RGATCY	7 cut(s)	598, 907, 1846, 2548, 2878, 2923, 3179
MhlI	GDGCHC	4 cut(s)	156, 3165, 3234, 3749
MlsI	TGGCCA	1 cut(s)	1479
MluNI	TGGCCA	1 cut(s)	1479
Mly113I	GGCGCC	1 cut(s)	2437
MlyI	GAGTC	8 cut(s)	92, 99, 1230, 1397, 1694, 2528, 2906, 2985
MmeI	TCCRAC	3 cut(s)	1722, 3334, 3516
Mox20I	TGGCCA	1 cut(s)	1479
Mph1103I	ATGCAT	4 cut(s)	1030, 2339, 3534, 3634
MroNI	GCCGGC	1 cut(s)	1950
MroXI	GAANNNNTTC	3 cut(s)	1113, 2256, 3020
MscI	TGGCCA	1 cut(s)	1479
MseI	TTAA	5 cut(s)	1055, 1434, 1533, 1959, 2292
MslI	CAYNNNNRTG	3 cut(s)	556, 744, 3608
Msp20I	TGGCCA	1 cut(s)	1479
MspA1I	CMGCKG	1 cut(s)	1910
MspI	CCGG	3 cut(s)	32, 94, 1951
MspR9I	CCNGG	6 cut(s)	464, 1393, 2383, 2441, 2811, 3772
MunI	CAATTG	3 cut(s)	938, 1089, 1497
Mva1269I	GAATGC	2 cut(s)	2303, 3532
MvaI	CCWGG	6 cut(s)	464, 1393, 2383, 2441, 2811, 3772
MvnI	CGCG	4 cut(s)	88, 210, 254, 2910
NaeI	GCCGGC	1 cut(s)	1952
NarI	GGCGCC	1 cut(s)	2437
NcoI	CCATGG	2 cut(s)	608, 3267
NdeI	CATATG	1 cut(s)	483
NgoMIV	GCCGGC	1 cut(s)	1950
NlaIV	GGNNCC	6 cut(s)	895, 1469, 2438, 2925, 3051, 3232
NmeAIII	GCCGAG	4 cut(s)	7, 175, 3184, 3374
NmuCI	GTSAC	6 cut(s)	14, 172, 1351, 2443, 2708, 3591
NruI	TCGCGA	1 cut(s)	88
NsiI	ATGCAT	4 cut(s)	1030, 2339, 3534, 3634
NspI	RCATGY	8 cut(s)	1571, 2420, 3114, 3240, 3409, 3435, 3543, 3607
OliI	CACNNNNGTG	2 cut(s)	556, 744
PaeI	GCATGC	1 cut(s)	2420
PaeR7I	CTCGAG	1 cut(s)	218
PceI	AGGCCT	1 cut(s)	3551
PciI	ACATGT	1 cut(s)	3539
PciSI	GCTCTTC	1 cut(s)	2307
PcsI	WCGNNNNNNNCGW	2 cut(s)	71, 464
PctI	GAATGC	2 cut(s)	2303, 3532
PdiI	GCCGGC	1 cut(s)	1952
PdmI	GAANNNNTTC	3 cut(s)	1113, 2256, 3020
PflMI	CCANNNNNTGG	2 cut(s)	484, 3584
PfoI	TCCNGGA	1 cut(s)	2809
PleI	GAGTC	8 cut(s)	91, 98, 1229, 1396, 1694, 2527, 2906, 2984
PluTI	GGCGCC	1 cut(s)	2440
PpsI	GAGTC	8 cut(s)	91, 98, 1229, 1396, 1694, 2527, 2906, 2984
Ppu21I	YACGTR	1 cut(s)	2571
PpuMI	RGGWCCY	1 cut(s)	3049
PscI	ACATGT	1 cut(s)	3539
PsiI	TTATAA	1 cut(s)	1268
Psp124BI	GAGCTC	1 cut(s)	156
Psp5II	RGGWCCY	1 cut(s)	3049
Psp6I	CCWGG	6 cut(s)	462, 1391, 2381, 2439, 2809, 3770
PspEI	GGTNACC	1 cut(s)	2443
PspFI	CCCAGC	1 cut(s)	1194
PspGI	CCWGG	6 cut(s)	462, 1391, 2381, 2439, 2809, 3770
PspN4I	GGNNCC	6 cut(s)	895, 1469, 2438, 2925, 3051, 3232
PspOMI	GGGCCC	1 cut(s)	3230
PspPI	GGNCC	8 cut(s)	727, 2071, 2173, 2283, 3049, 3230, 3231, 3508
PspPPI	RGGWCCY	1 cut(s)	3049
PsrI	GAACNNNNNNTAC	2 cut(s)	1567, 1599
PstI	CTGCAG	1 cut(s)	525
PstNI	CAGNNNCTG	1 cut(s)	2956
PsuI	RGATCY	7 cut(s)	598, 907, 1846, 2548, 2878, 2923, 3179
PvuII	CAGCTG	1 cut(s)	1910
RruI	TCGCGA	1 cut(s)	88
RsaI	GTAC	6 cut(s)	263, 331, 675, 1082, 1576, 2573
RsaNI	GTAC	6 cut(s)	262, 330, 674, 1081, 1575, 2572
RseI	CAYNNNNRTG	3 cut(s)	556, 744, 3608
SacI	GAGCTC	1 cut(s)	156
SapI	GCTCTTC	1 cut(s)	2307
SaqAI	TTAA	5 cut(s)	1055, 1434, 1533, 1959, 2292
Sau96I	GGNCC	8 cut(s)	727, 2071, 2173, 2283, 3049, 3230, 3231, 3508
ScaI	AGTACT	1 cut(s)	1576
SchI	GAGTC	8 cut(s)	92, 99, 1230, 1397, 1694, 2528, 2906, 2985
ScrFI	CCNGG	6 cut(s)	464, 1393, 2383, 2441, 2811, 3772
SduI	GDGCHC	4 cut(s)	156, 3165, 3234, 3749
SfcI	CTRYAG	4 cut(s)	521, 1018, 1279, 2544
SfoI	GGCGCC	1 cut(s)	2438
Sfr274I	CTCGAG	1 cut(s)	218
SinI	GGWCC	3 cut(s)	2071, 2173, 3049
SlaI	CTCGAG	1 cut(s)	218
SmiMI	CAYNNNNRTG	3 cut(s)	556, 744, 3608
SmlI	CTYRAG	5 cut(s)	218, 245, 779, 1729, 1810
SmoI	CTYRAG	5 cut(s)	218, 245, 779, 1729, 1810
SnaBI	TACGTA	1 cut(s)	2571
SphI	GCATGC	1 cut(s)	2420
SseBI	AGGCCT	1 cut(s)	3551
SsiI	CCGC	6 cut(s)	6, 166, 183, 208, 1225, 2908
SspDI	GGCGCC	1 cut(s)	2436
SspI	AATATT	2 cut(s)	1530, 2364
SspMI	CTAG	9 cut(s)	296, 302, 593, 911, 962, 2081, 2117, 2594, 2730
SstI	GAGCTC	1 cut(s)	156
StuI	AGGCCT	1 cut(s)	3551
StyD4I	CCNGG	6 cut(s)	462, 1391, 2381, 2439, 2809, 3770
StyI	CCWWGG	7 cut(s)	186, 608, 1900, 2022, 2553, 2919, 3267
TaiI	ACGT	5 cut(s)	174, 1724, 2237, 2573, 3491
TaqI	TCGA	4 cut(s)	219, 736, 1612, 2963
TatI	WGTACW	2 cut(s)	329, 1574
TauI	GCSGC	3 cut(s)	9, 185, 210
Tru1I	TTAA	5 cut(s)	1055, 1434, 1533, 1959, 2292
Tru9I	TTAA	5 cut(s)	1055, 1434, 1533, 1959, 2292
TscAI	CASTG	4 cut(s)	272, 748, 1633, 2680
TseFI	GTSAC	6 cut(s)	14, 172, 1351, 2443, 2708, 3591
TseI	GCWGC	8 cut(s)	523, 589, 1407, 1417, 1522, 1928, 3107, 3141
Tsp45I	GTSAC	6 cut(s)	14, 172, 1351, 2443, 2708, 3591
TspGWI	ACGGA	2 cut(s)	1460, 3027
TspRI	CASTG	4 cut(s)	272, 748, 1633, 2680
Van91I	CCANNNNNTGG	2 cut(s)	484, 3584
VneI	GTGCAC	1 cut(s)	3745
VpaK11BI	GGWCC	3 cut(s)	2071, 2173, 3049
XapI	RAATTY	8 cut(s)	428, 1132, 1175, 1199, 1504, 3020, 3216, 3297
XbaI	TCTAGA	3 cut(s)	910, 961, 2116
XceI	RCATGY	8 cut(s)	1571, 2420, 3114, 3240, 3409, 3435, 3543, 3607
XcmI	CCANNNNNNNNNTGG	1 cut(s)	851
XhoI	CTCGAG	1 cut(s)	218
XmiI	GTMKAC	1 cut(s)	1752
XmnI	GAANNNNTTC	3 cut(s)	1113, 2256, 3020
XspI	CTAG	9 cut(s)	296, 302, 593, 911, 962, 2081, 2117, 2594, 2730
ZraI	GACGTC	1 cut(s)	1722
ZrmI	AGTACT	1 cut(s)	1576
Zsp2I	ATGCAT	4 cut(s)	1030, 2339, 3534, 3634

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.