Rh1BG240300

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002430
Physical Location & Seq
Forward (+)
36324562 .. 36327904
3343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG240300.1

Sequence Viewer

Length: 561 bp
ATGGCCGAGCACAGTGAGAGCGCCGATGCTAAGGTGGAGCAGACGATGATGGAGAAGATCAGCGAGAAGATCCACGATCATCACGACTCTTCGTCGTCGTCGTCTTCCGACTCCGACACCGAGAAGCCGGCGCCGGCCACGGAGGAGTCGGCGGTCAAGGAGAAGGTGTTCCGTCTCTTCGGAAGGGAGCGTCCTGTTCATCATGTCTTTGGCGGTGGAAAGCCTGCTGATGTGTTCTTGTGGAGGAACAAGAAACTTTCGGCTGGGGTTCTTGGAGGAGCTACTGCTGTTTATGTCCTTTTTGAGTTGCTTCAATACCACTTGCTTACGCTTGTCTGCCACATTCTGGTTGGTACCCTAGCAATCTTGTTTCTGTGGTCCAATGCCCATACTATTATCCACAAGACTCCACCACGTATCCCAGAGGTTTGTCTTCCTGAGGAGCCATTCCTGCAGGTTGCTTCTGAGCTGAGACATCAAATCAACTGGACTTTTGCACAACTTCATAACATTGCATCTGGAAGGGAGTTGAAGAAATTCCTGATTGTACGTGTATATTGA

Protein Analysis

186

Amino Acids

20.92

Weight (kDa)

6.63

Isoelectric Point (pI)

57.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 76 - 183 7.5e-33 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 445
Acc65I GGTACC 1 cut(s) 353
AccB1I GGYRCC 2 cut(s) 130, 353
AccB7I CCANNNNNTGG 1 cut(s) 346
AciI CCGC 2 cut(s) 152, 213
AclWI GGATC 1 cut(s) 64
AcoI YGGCCR 2 cut(s) 3, 135
AcsI RAATTY 1 cut(s) 536
AcyI GRCGYC 1 cut(s) 131
AfaI GTAC 2 cut(s) 355, 549
AfiI CCNNNNNNNGG 1 cut(s) 346
AflIII ACRYGT 1 cut(s) 550
AgsI TTSAA 2 cut(s) 314, 532
AhdI GACNNNNNGTC 1 cut(s) 91
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 2 cut(s) 281, 469
AluI AGCT 2 cut(s) 281, 469
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 2 cut(s) 179, 466
AlwI GGATC 1 cut(s) 64
AoxI GGCC 2 cut(s) 3, 135
ApoI RAATTY 1 cut(s) 536
Asp700I GAANNNNTTC 2 cut(s) 167, 536
Asp718I GGTACC 1 cut(s) 353
AspLEI GCGC 2 cut(s) 23, 133
AspS9I GGNCC 1 cut(s) 378
AvaII GGWCC 1 cut(s) 378
AxyI CCTNAGG 1 cut(s) 438
BanI GGYRCC 2 cut(s) 130, 353
BbsI GAAGAC 2 cut(s) 96, 425
Bbv12I GWGCWC 1 cut(s) 12
BccI CCATC 1 cut(s) 43
BciVI GTATCC 1 cut(s) 428
BcoDI GTCTC 2 cut(s) 179, 466
BfaI CTAG 1 cut(s) 359
BfmI CTRYAG 1 cut(s) 452
BfoI RGCGCY 2 cut(s) 24, 134
BfuAI ACCTGC 1 cut(s) 445
BfuI GTATCC 1 cut(s) 428
Bme18I GGWCC 1 cut(s) 378
BmeRI GACNNNNNGTC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 378
BmiI GGNNCC 3 cut(s) 132, 355, 444
BmsI GCATC 2 cut(s) 16, 524
BpiI GAAGAC 2 cut(s) 96, 425
Bpu10I CCTNAGC 1 cut(s) 30
BsaAI YACGTR 2 cut(s) 416, 551
BsaHI GRCGYC 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 138
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse118I RCCGGY 2 cut(s) 127, 133
Bse1I ACTGG 1 cut(s) 491
Bse21I CCTNAGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 510
BseDI CCNNGG 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 3 cut(s) 429, 456, 461
BseNI ACTGG 1 cut(s) 491
BseRI GAGGAG 3 cut(s) 158, 291, 455
BseYI CCCAGC 1 cut(s) 263
BshFI GGCC 2 cut(s) 5, 137
BshNI GGYRCC 2 cut(s) 130, 353
BsiHKAI GWGCWC 1 cut(s) 12
BsiSI CCGG 2 cut(s) 128, 134
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 2 cut(s) 179, 466
BsmBI CGTCTC 1 cut(s) 179
BsnI GGCC 2 cut(s) 5, 137
Bsp1286I GDGCHC 1 cut(s) 12
Bsp143I GATC 3 cut(s) 57, 69, 76
BspACI CCGC 2 cut(s) 152, 213
BspANI GGCC 2 cut(s) 5, 137
BspCNI CTCAG 3 cut(s) 430, 457, 462
BspLI GGNNCC 3 cut(s) 132, 355, 444
BspMAI CTGCAG 1 cut(s) 456
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 1 cut(s) 64
BspT107I GGYRCC 2 cut(s) 130, 353
BsrDI GCAATG 1 cut(s) 510
BsrFI RCCGGY 2 cut(s) 127, 133
BsrI ACTGG 1 cut(s) 491
BssAI RCCGGY 2 cut(s) 127, 133
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 3 cut(s) 57, 69, 76
BssNI GRCGYC 1 cut(s) 131
Bst4CI ACNGT 1 cut(s) 14
Bst6I CTCTTC 2 cut(s) 94, 182
BstACI GRCGYC 1 cut(s) 131
BstBAI YACGTR 2 cut(s) 416, 551
BstC8I GCNNGC 3 cut(s) 129, 135, 225
BstDEI CTNAG 4 cut(s) 30, 438, 465, 470
BstDSI CCRYGG 1 cut(s) 138
BstH2I RGCGCY 2 cut(s) 24, 134
BstHHI GCGC 2 cut(s) 23, 133
BstKTI GATC 3 cut(s) 60, 72, 79
BstMAI GTCTC 2 cut(s) 179, 466
BstMBI GATC 3 cut(s) 57, 69, 76
BstMWI GCNNNNNNNGC 1 cut(s) 451
BstSFI CTRYAG 1 cut(s) 452
BstV2I GAAGAC 2 cut(s) 96, 425
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
Bsu36I CCTNAGG 1 cut(s) 438
BsuI GTATCC 1 cut(s) 428
BsuRI GGCC 2 cut(s) 5, 137
BtgI CCRYGG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 19
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 3 cut(s) 129, 135, 225
CfoI GCGC 2 cut(s) 23, 133
Cfr10I RCCGGY 2 cut(s) 127, 133
Cfr13I GGNCC 1 cut(s) 378
CseI GACGC 1 cut(s) 179
Csp6I GTAC 2 cut(s) 354, 548
CviAII CATG 1 cut(s) 203
CviJI RGCY 8 cut(s) 5, 127, 137, 223, 263, 281, 445, 469
CviKI_1 RGCY 8 cut(s) 5, 127, 137, 223, 263, 281, 445, 469
CviQI GTAC 2 cut(s) 354, 548
DdeI CTNAG 4 cut(s) 30, 438, 465, 470
DinI GGCGCC 1 cut(s) 132
DpnI GATC 3 cut(s) 59, 71, 78
DpnII GATC 3 cut(s) 57, 69, 76
DriI GACNNNNNGTC 1 cut(s) 91
EaeI YGGCCR 2 cut(s) 3, 135
Eam1104I CTCTTC 2 cut(s) 94, 182
Eam1105I GACNNNNNGTC 1 cut(s) 91
EarI CTCTTC 2 cut(s) 94, 182
Eco47I GGWCC 1 cut(s) 378
Eco81I CCTNAGG 1 cut(s) 438
EgeI GGCGCC 1 cut(s) 132
EheI GGCGCC 1 cut(s) 132
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 1 cut(s) 206
FaiI YATR 5 cut(s) 204, 294, 390, 507, 556
FatI CATG 1 cut(s) 202
FspBI CTAG 1 cut(s) 359
GlaI GCGC 2 cut(s) 22, 132
GsaI CCCAGC 1 cut(s) 267
HaeII RGCGCY 2 cut(s) 24, 134
HaeIII GGCC 2 cut(s) 5, 137
HapII CCGG 2 cut(s) 128, 134
HgaI GACGC 1 cut(s) 179
HhaI GCGC 2 cut(s) 23, 133
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 1 cut(s) 206
Hin6I GCGC 2 cut(s) 21, 131
HinP1I GCGC 2 cut(s) 21, 131
HinfI GANTC 4 cut(s) 86, 110, 146, 406
HpaII CCGG 2 cut(s) 128, 134
Hpy188I TCNGA 4 cut(s) 109, 115, 182, 466
Hpy188III TCNNGA 4 cut(s) 83, 437, 519, 541
Hpy99I CGWCG 3 cut(s) 97, 100, 103
HpyAV CCTTC 3 cut(s) 157, 177, 516
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4IV ACGT 2 cut(s) 415, 550
HpyCH4V TGCA 3 cut(s) 454, 497, 515
HpyF10VI GCNNNNNNNGC 1 cut(s) 451
HpyF3I CTNAG 4 cut(s) 30, 438, 465, 470
HpySE526I ACGT 2 cut(s) 415, 550
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 1 cut(s) 206
HspAI GCGC 2 cut(s) 21, 131
KasI GGCGCC 1 cut(s) 130
KpnI GGTACC 1 cut(s) 357
KroI GCCGGC 2 cut(s) 127, 133
KroNI GCCGGC 2 cut(s) 129, 135
Kzo9I GATC 3 cut(s) 57, 69, 76
LmnI GCTCC 4 cut(s) 37, 187, 278, 442
LweI GCATC 2 cut(s) 16, 524
MaeI CTAG 1 cut(s) 359
MaeII ACGT 2 cut(s) 415, 550
MalI GATC 3 cut(s) 59, 71, 78
MboI GATC 3 cut(s) 57, 69, 76
MboII GAAGA 7 cut(s) 67, 79, 81, 96, 169, 425, 544
MflI RGATCY 1 cut(s) 69
MhlI GDGCHC 1 cut(s) 12
MluCI AATT 1 cut(s) 536
Mly113I GGCGCC 1 cut(s) 131
MlyI GAGTC 4 cut(s) 80, 104, 155, 400
MmeI TCCRAC 2 cut(s) 132, 138
MnlI CCTC 5 cut(s) 136, 237, 269, 418, 433
MroNI GCCGGC 2 cut(s) 127, 133
MroXI GAANNNNTTC 2 cut(s) 167, 536
MspI CCGG 2 cut(s) 128, 134
MwoI GCNNNNNNNGC 1 cut(s) 451
NaeI GCCGGC 2 cut(s) 129, 135
NarI GGCGCC 1 cut(s) 131
NdeII GATC 3 cut(s) 57, 69, 76
NgoMIV GCCGGC 2 cut(s) 127, 133
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 3 cut(s) 132, 355, 444
NmeAIII GCCGAG 1 cut(s) 31
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 98
PdiI GCCGGC 2 cut(s) 129, 135
PdmI GAANNNNTTC 2 cut(s) 167, 536
PflMI CCANNNNNTGG 1 cut(s) 346
PleI GAGTC 4 cut(s) 80, 104, 154, 400
PluTI GGCGCC 1 cut(s) 134
PpsI GAGTC 4 cut(s) 80, 104, 154, 400
Ppu21I YACGTR 2 cut(s) 416, 551
PspFI CCCAGC 1 cut(s) 263
PspN4I GGNNCC 3 cut(s) 132, 355, 444
PspPI GGNCC 1 cut(s) 378
PstI CTGCAG 1 cut(s) 456
PsuI RGATCY 1 cut(s) 69
RsaI GTAC 2 cut(s) 355, 549
RsaNI GTAC 2 cut(s) 354, 548
Sau3AI GATC 3 cut(s) 57, 69, 76
Sau96I GGNCC 1 cut(s) 378
SbfI CCTGCAGG 1 cut(s) 456
SchI GAGTC 4 cut(s) 80, 104, 155, 400
SdaI CCTGCAGG 1 cut(s) 456
SduI GDGCHC 1 cut(s) 12
SetI ASST 8 cut(s) 36, 168, 283, 418, 429, 459, 471, 553
SfaNI GCATC 2 cut(s) 16, 524
SfcI CTRYAG 1 cut(s) 452
SfoI GGCGCC 1 cut(s) 132
SinI GGWCC 1 cut(s) 378
Sse8387I CCTGCAGG 1 cut(s) 456
Sse9I AATT 1 cut(s) 536
SsiI CCGC 2 cut(s) 152, 213
SspDI GGCGCC 1 cut(s) 130
SspMI CTAG 1 cut(s) 359
TaaI ACNGT 1 cut(s) 14
TaiI ACGT 2 cut(s) 418, 553
TasI AATT 1 cut(s) 536
TscAI CASTG 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 188, 494
TspGWI ACGGA 2 cut(s) 155, 161
TspRI CASTG 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 346
VpaK11BI GGWCC 1 cut(s) 378
XapI RAATTY 1 cut(s) 536
XcmI CCANNNNNNNNNTGG 1 cut(s) 347
XmnI GAANNNNTTC 2 cut(s) 167, 536
XspI CTAG 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.