MD14G1115200.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002430
Physical Location & Seq
Forward (+)
18719809 .. 18721962
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1115200.v1.1.491

Sequence Viewer

Length: 777 bp
ATGGCGGAGCATGCAGACGAAGTGAAGGCGGCGGAGTCTTTGTTGGACAAGGTACCGGACAAGATCCACGGTCACGATTCGTCGTCGGCGTTACCGCCGTCGGAGGCAGTGCGCGACAAGGGCATTGGCGGTGGCTATTCGGCATCGTTGAAGTCCAAGGTTAACAGGCTCTTTGGGAGGGAGAAGCCCGTGCACCACGTCTTCGGTGGCGGAAAAGCCGCTGATATTTTCCTTTGGAGGGGCAAGAAGGTGTCGGCAGGTGTTCTTATCGCCTTAACGATCATATGGGTTTTCTTTGAATTGCTTGAATACCATCTGCTGACTCTGGTCTCCCACTTGTTGATTATTGGGATTGTTATGTTGTTCTTGTGGTCCAACGCTTCAACCTTCATTAACAAGTCTCCACCCCACATTCCACAAGTTCAAATTCCTGAGAAGCCGGTTCTACAGATTGCGTCTGCAATTACATCAGAGATCAACCGTGCTTTTGTTATCGTGCGGGAGATTGCATCTGGGAAGGATCTGAAGCAGTTCCTCTCTGCGATTGCTGGCTTGTGGGTTGTGTCCATCTTGGGGAAGTACTACAACTTCTTGACCTTGGTCTACATAGCGGTTGTTTTGCTCTTCACACTTCCTGTGTTCTATGAGAAATACGATGACCAGGTGGATGCGGTTGCGGAGAAGGCACTGACTGAGATGAAGAAGCAGTATGCAGTATTTGACGCAAAGGTGCTAAGTAAAATTCCTAGGGGGCAGACGAAGGCCAAGAAGTTTTAG

Protein Analysis

259

Amino Acids

28.56

Weight (kDa)

9.36

Isoelectric Point (pI)

29.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 74 - 229 1.4e-55 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 248
Acc65I GGTACC 1 cut(s) 52
AccB1I GGYRCC 1 cut(s) 52
AccI GTMKAC 1 cut(s) 603
AccII CGCG 1 cut(s) 114
AclWI GGATC 2 cut(s) 58, 528
AcsI RAATTY 2 cut(s) 426, 741
AcuI CTGAAG 1 cut(s) 545
AfaI GTAC 2 cut(s) 54, 581
AfiI CCNNNNNNNGG 2 cut(s) 238, 573
AgsI TTSAA 5 cut(s) 151, 299, 308, 384, 425
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 1 cut(s) 660
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 2 cut(s) 334, 405
Alw44I GTGCAC 1 cut(s) 191
AlwI GGATC 2 cut(s) 58, 528
AoxI GGCC 1 cut(s) 762
ApaLI GTGCAC 1 cut(s) 191
ApoI RAATTY 2 cut(s) 426, 741
Asp700I GAANNNNTTC 1 cut(s) 530
Asp718I GGTACC 1 cut(s) 52
AspA2I CCTAGG 1 cut(s) 746
AspLEI GCGC 1 cut(s) 114
AspS9I GGNCC 1 cut(s) 372
AvaII GGWCC 1 cut(s) 372
AvrII CCTAGG 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 195
BanI GGYRCC 1 cut(s) 52
BarI GAAGNNNNNNTAC 2 cut(s) 572, 604
BbsI GAAGAC 1 cut(s) 193
Bbv12I GWGCWC 1 cut(s) 195
BccI CCATC 2 cut(s) 321, 575
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 662
BcoDI GTCTC 2 cut(s) 334, 405
BfaI CTAG 1 cut(s) 747
BfmI CTRYAG 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 248
BisI GCNGC 2 cut(s) 30, 219
BlnI CCTAGG 1 cut(s) 746
BlsI GCNGC 2 cut(s) 31, 220
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 1 cut(s) 662
Bme18I GGWCC 1 cut(s) 372
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 372
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 662
BmsI GCATC 3 cut(s) 152, 518, 658
BoxI GACNNNNGTC 2 cut(s) 326, 599
BpiI GAAGAC 1 cut(s) 193
BsaI GGTCTC 1 cut(s) 334
BsaJI CCNNGG 4 cut(s) 67, 156, 597, 746
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 573
Bse118I RCCGGY 1 cut(s) 439
BseBI CCWGG 1 cut(s) 662
BseDI CCNNGG 4 cut(s) 67, 156, 597, 746
BseGI GGATG 1 cut(s) 673
BseLI CCNNNNNNNGG 2 cut(s) 238, 573
BseMII CTCAG 2 cut(s) 423, 684
BseSI GKGCMC 1 cut(s) 195
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 764
BshNI GGYRCC 1 cut(s) 52
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 2 cut(s) 56, 440
BslI CCNNNNNNNGG 2 cut(s) 238, 573
BsmAI GTCTC 2 cut(s) 334, 405
BsnI GGCC 1 cut(s) 764
Bso31I GGTCTC 1 cut(s) 334
Bsp1286I GDGCHC 1 cut(s) 195
Bsp143I GATC 4 cut(s) 63, 279, 474, 520
BspANI GGCC 1 cut(s) 764
BspCNI CTCAG 2 cut(s) 424, 685
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 54
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 2 cut(s) 58, 528
BspQI GCTCTTC 1 cut(s) 629
BspT107I GGYRCC 1 cut(s) 52
BspTNI GGTCTC 1 cut(s) 334
BsrFI RCCGGY 1 cut(s) 439
BssAI RCCGGY 1 cut(s) 439
BssECI CCNNGG 4 cut(s) 67, 156, 597, 746
BssMI GATC 4 cut(s) 63, 279, 474, 520
BssT1I CCWWGG 3 cut(s) 156, 597, 746
Bst2UI CCWGG 1 cut(s) 662
Bst4CI ACNGT 2 cut(s) 71, 482
Bst6I CTCTTC 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 12, 550
BstDEI CTNAG 3 cut(s) 432, 693, 734
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 1 cut(s) 673
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 4 cut(s) 66, 282, 477, 523
BstMAI GTCTC 2 cut(s) 334, 405
BstMBI GATC 4 cut(s) 63, 279, 474, 520
BstMWI GCNNNNNNNGC 3 cut(s) 11, 120, 683
BstNI CCWGG 1 cut(s) 662
BstNSI RCATGY 1 cut(s) 14
BstPAI GACNNNNGTC 2 cut(s) 326, 599
BstSCI CCNGG 1 cut(s) 660
BstSFI CTRYAG 1 cut(s) 446
BstSLI GKGCMC 1 cut(s) 195
BstUI CGCG 1 cut(s) 114
BstV2I GAAGAC 1 cut(s) 193
BstX2I RGATCY 2 cut(s) 63, 520
BstYI RGATCY 2 cut(s) 63, 520
BsuRI GGCC 1 cut(s) 764
BtgI CCRYGG 1 cut(s) 67
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 1 cut(s) 673
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 2 cut(s) 114, 686
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 2 cut(s) 12, 550
CfoI GCGC 1 cut(s) 114
Cfr10I RCCGGY 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 372
CseI GACGC 2 cut(s) 444, 731
CsiI ACCWGGT 1 cut(s) 660
Csp6I GTAC 2 cut(s) 53, 580
CviAII CATG 1 cut(s) 11
CviJI RGCY 7 cut(s) 135, 169, 187, 218, 439, 552, 764
CviKI_1 RGCY 7 cut(s) 135, 169, 187, 218, 439, 552, 764
CviQI GTAC 2 cut(s) 53, 580
DdeI CTNAG 3 cut(s) 432, 693, 734
DpnI GATC 4 cut(s) 65, 281, 476, 522
DpnII GATC 4 cut(s) 63, 279, 474, 520
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
EciI GGCGGA 3 cut(s) 20, 47, 225
Eco130I CCWWGG 3 cut(s) 156, 597, 746
Eco31I GGTCTC 1 cut(s) 334
Eco47I GGWCC 1 cut(s) 372
Eco57I CTGAAG 1 cut(s) 545
EcoRII CCWGG 1 cut(s) 660
EcoT14I CCWWGG 3 cut(s) 156, 597, 746
ErhI CCWWGG 3 cut(s) 156, 597, 746
FaeI CATG 1 cut(s) 14
FaiI YATR 7 cut(s) 12, 284, 286, 359, 608, 645, 711
FatI CATG 1 cut(s) 10
FauI CCCGC 1 cut(s) 492
FauNDI CATATG 1 cut(s) 284
FblI GTMKAC 1 cut(s) 603
Fnu4HI GCNGC 2 cut(s) 30, 219
FokI GGATG 1 cut(s) 680
Fsp4HI GCNGC 2 cut(s) 30, 219
FspBI CTAG 1 cut(s) 747
GlaI GCGC 1 cut(s) 113
GluI GCNGC 2 cut(s) 30, 219
HaeIII GGCC 1 cut(s) 764
HapII CCGG 2 cut(s) 56, 440
HgaI GACGC 2 cut(s) 444, 731
HhaI GCGC 1 cut(s) 114
Hin1II CATG 1 cut(s) 14
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 3 cut(s) 35, 77, 322
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 2 cut(s) 56, 440
Hpy166II GTNNAC 3 cut(s) 163, 193, 604
Hpy188I TCNGA 3 cut(s) 103, 472, 525
Hpy188III TCNNGA 3 cut(s) 74, 431, 592
Hpy8I GTNNAC 3 cut(s) 163, 193, 604
Hpy99I CGWCG 3 cut(s) 85, 88, 103
HpyAV CCTTC 6 cut(s) 19, 241, 397, 511, 676, 754
HpyCH4III ACNGT 2 cut(s) 71, 482
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 5 cut(s) 14, 193, 461, 509, 713
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 120, 683
HpyF3I CTNAG 3 cut(s) 432, 693, 734
HpySE526I ACGT 1 cut(s) 198
Hsp92II CATG 1 cut(s) 14
HspAI GCGC 1 cut(s) 112
KpnI GGTACC 1 cut(s) 56
KspAI GTTAAC 1 cut(s) 163
Kzo9I GATC 4 cut(s) 63, 279, 474, 520
LguI GCTCTTC 1 cut(s) 629
LmnI GCTCC 1 cut(s) 7
LweI GCATC 3 cut(s) 152, 518, 658
MabI ACCWGGT 1 cut(s) 660
MaeI CTAG 1 cut(s) 747
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 2 cut(s) 71, 90
MalI GATC 4 cut(s) 65, 281, 476, 522
MboI GATC 4 cut(s) 63, 279, 474, 520
MboII GAAGA 3 cut(s) 193, 616, 712
MflI RGATCY 2 cut(s) 63, 520
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 4 cut(s) 299, 426, 462, 741
MlyI GAGTC 2 cut(s) 44, 316
MmeI TCCRAC 3 cut(s) 24, 81, 399
MnlI CCTC 4 cut(s) 97, 171, 231, 545
MroXI GAANNNNTTC 1 cut(s) 530
MseI TTAA 3 cut(s) 162, 275, 393
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 2 cut(s) 56, 440
MspR9I CCNGG 1 cut(s) 662
MvaI CCWGG 1 cut(s) 662
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 3 cut(s) 11, 120, 683
NdeI CATATG 1 cut(s) 284
NdeII GATC 4 cut(s) 63, 279, 474, 520
NlaIII CATG 1 cut(s) 14
NlaIV GGNNCC 1 cut(s) 54
NmuCI GTSAC 1 cut(s) 71
NspI RCATGY 1 cut(s) 14
PaeI GCATGC 1 cut(s) 14
PaqCI CACCTGC 1 cut(s) 248
PciSI GCTCTTC 1 cut(s) 629
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 2 cut(s) 31, 220
PleI GAGTC 2 cut(s) 43, 316
PpsI GAGTC 2 cut(s) 43, 316
PshAI GACNNNNGTC 2 cut(s) 326, 599
Psp6I CCWGG 1 cut(s) 660
PspGI CCWGG 1 cut(s) 660
PspN4I GGNNCC 1 cut(s) 54
PspPI GGNCC 1 cut(s) 372
PsuI RGATCY 2 cut(s) 63, 520
RsaI GTAC 2 cut(s) 54, 581
RsaNI GTAC 2 cut(s) 53, 580
SapI GCTCTTC 1 cut(s) 629
SaqAI TTAA 3 cut(s) 162, 275, 393
SatI GCNGC 2 cut(s) 30, 219
Sau3AI GATC 4 cut(s) 63, 279, 474, 520
Sau96I GGNCC 1 cut(s) 372
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 2 cut(s) 44, 316
ScrFI CCNGG 1 cut(s) 662
SduI GDGCHC 1 cut(s) 195
SetI ASST 9 cut(s) 54, 162, 201, 252, 262, 389, 599, 666, 732
SexAI ACCWGGT 1 cut(s) 660
SfaNI GCATC 3 cut(s) 152, 518, 658
SfcI CTRYAG 1 cut(s) 446
SinI GGWCC 1 cut(s) 372
SphI GCATGC 1 cut(s) 14
Sse9I AATT 4 cut(s) 299, 426, 462, 741
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 1 cut(s) 660
StyI CCWWGG 3 cut(s) 156, 597, 746
TaaI ACNGT 2 cut(s) 71, 482
TaiI ACGT 1 cut(s) 201
TasI AATT 4 cut(s) 299, 426, 462, 741
TatI WGTACW 1 cut(s) 579
TauI GCSGC 2 cut(s) 32, 221
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 3 cut(s) 162, 275, 393
Tru9I TTAA 3 cut(s) 162, 275, 393
TscAI CASTG 2 cut(s) 114, 693
TseFI GTSAC 1 cut(s) 71
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 2 cut(s) 379, 713
TspRI CASTG 2 cut(s) 114, 693
VneI GTGCAC 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 372
XapI RAATTY 2 cut(s) 426, 741
XceI RCATGY 1 cut(s) 14
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmaJI CCTAGG 1 cut(s) 746
XmiI GTMKAC 1 cut(s) 603
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 1 cut(s) 747
ZrmI AGTACT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.